Objective To study the effects of mouse UNCV protein on apoptosis in HeLa cells.Methods The recombinant plasmids containing Uncv gene of BALB/C mice were transfected into HeLa cells.The HeLa cells stably overexpressing UNCV protein were selected.The effects of overexpressed UNCV protein on apoptosis in HeLa cells induced by doxorubicin and serum starvation were measured by cell counting and flow cytometry.Results HeLa cells stably overexpressing UNCV protein were obtained.The results of cell counting and flow cytometry showed that the overexpression of UNCV protein inhibited apoptosis in HeLa cells induced by serum starvation.The effects of UNCV protein on apoptosis in HeLa cells induced by doxorubicin were not significantly different.Conclusions Overexpression of UNCV protein inhibits apoptosis in HeLa cells induced by serum starvation.

Objective To clone the full length cDNA of Uncv gene in mice and to express the gene in eukaryotic cells.Methods RT-PCR assay was applied to clone the full length coding region of the Uncv gene and constructed its expression plasmid pcDNA 3.1-Flag/Uncv.The recombinant plasmid was transfected into HeLa cells and the fusion protein was identified by Western blot analysis.Results The complete coding sequence was obtained and cloned into the pcDNA 3.1-Flag vector.The recombinant pcDNA 3.1-Flag/Uncv plasmid was transiently expressed in HeLa cells.HeLa cell clones expressing fusion protein with molecular weight of about 95×103 were obtained.Conclusions A recombinant eukaryotic expression plasmid of Uncv has been successfully constructed and expressed in HeLa cells.It may provide a foundation for further biological studies of Uncv gene.