利用潜水镜成像机理,研究了水中物点成像规律,给出了物点与像点的坐标关系以及平面和球面潜水镜的成像畸变规律.研究表明:平面潜水镜成像同时存在较大的横向畸变和纵向畸变,而球面潜水镜可以有效抑制横向畸变,但是对于纵向畸变无校正效果.

Objective To construct and identify retroviral-mediated short hairpin RNA ( shRNA ) expression vectors of ERβ419, and explore ERβ419 unknown biological function in beagles in future.Methods To screen out the most effective gene silencing sequence of beagle ERβ419 mRNA using qRT-PCR and Western Blot assays, imitate beagle estrogen target cells.Results qRT-PCR results showed, ERβ419-shRNA1 ( P <0.01 ) and ERβ419-shRNA3 ( P <0.01)differed significantly, Western Blot result as same as qRT-PCR,ERβ419-shRNA3 is the best choice.Conclusion Beagles ERβ419-shRNA3 retrain most effectively target gene repression. It is applied to explore ERβ419 unknown biological function in beagles reproductive system, and to prevent and treat beagles reproductive function diseases.

Objective Atfu-B* sv1 with α2 domain deletion is a splice variant of major histocompatibility complex class I B in Ateles fusciceps.The aim of this study was to obtain the basic information of expression and intracellular localization of Atfu-B* sv1,and to compared them with that of the full-length Atfu-B gene.Methods Eukaryotic expression vectors of Atfu-B* sv1,Atfu B* 02:03 and Atfu-B* 03:01 were constructed and transfected into 293T cells,respectively.Expression of the three genes in the cells was detected by Western blotting,and the intracellular localization of the three genes was analyzed by immunofluorescence and laser confocal microscopy.Results Atfu-B* sv1,Atfu-B* 02:03 and AtfuB* 03:01 were all expressed on the plasma membrane of 293T cells and were glycosylated.Conclusions The expression and intracellular localization of Atfu-B* sv1 is not significantly different from those of full length Atfu-B genes.Further studies on the functions of Atfu-B* sv1 are needed to be done.

Objective To study the effects of mouse UNCV protein on apoptosis in HeLa cells.Methods The recombinant plasmids containing Uncv gene of BALB/C mice were transfected into HeLa cells.The HeLa cells stably overexpressing UNCV protein were selected.The effects of overexpressed UNCV protein on apoptosis in HeLa cells induced by doxorubicin and serum starvation were measured by cell counting and flow cytometry.Results HeLa cells stably overexpressing UNCV protein were obtained.The results of cell counting and flow cytometry showed that the overexpression of UNCV protein inhibited apoptosis in HeLa cells induced by serum starvation.The effects of UNCV protein on apoptosis in HeLa cells induced by doxorubicin were not significantly different.Conclusions Overexpression of UNCV protein inhibits apoptosis in HeLa cells induced by serum starvation.

目的 建立BABL/c小鼠感染大肠杆菌O127的模型及TGF-β1的荧光定量PCR方法检测方法.方法 通过灌胃的方式建立肠炎性小鼠模型,并利用嵌合荧光定量的方法对其结肠TGF-β1定量分析实验.结果 成功建立了肠炎性小鼠模型,荧光定量PCR试验结果表明实验组TGF-p1表达量明显高于对照组.结论 利用经口灌胃的方法可以成功的建立肠炎性小鼠动物模型,TGF-β1定量方法可以作为模型建立的评价标准.

目的探讨培美曲塞单药或联合顺铂治疗经化疗及EGFR-TKI失败的晚期复发性NSCLC患者的疗效及不良反应。方法经病理学或细胞学确诊的经化疗及EGFR-TKI治疗失败的晚期复发性NSCLC患者10例。单药治疗:培美曲塞500mg/m2第1天,按1周期21天方案进行,联合治疗:培美曲塞500mg/m2第1天+顺铂75mg/m2分第1、第2、第3天给药,按1周期21天方案进行。结果全组10例,无CR病例,PR 2例,SD 4例,PD 4例,疾病控制率60%。中位疾病进展时间11.4周。结论培美曲塞对于常规化疗、靶向治疗(EGFR-TKI类)失败的二线以上的晚期非小细胞肺癌仍有一定疗效。不良反应较轻,整体耐受性较好。

Objective To clone the full length cDNA of Uncv gene in mice and to express the gene in eukaryotic cells.Methods RT-PCR assay was applied to clone the full length coding region of the Uncv gene and constructed its expression plasmid pcDNA 3.1-Flag/Uncv.The recombinant plasmid was transfected into HeLa cells and the fusion protein was identified by Western blot analysis.Results The complete coding sequence was obtained and cloned into the pcDNA 3.1-Flag vector.The recombinant pcDNA 3.1-Flag/Uncv plasmid was transiently expressed in HeLa cells.HeLa cell clones expressing fusion protein with molecular weight of about 95×103 were obtained.Conclusions A recombinant eukaryotic expression plasmid of Uncv has been successfully constructed and expressed in HeLa cells.It may provide a foundation for further biological studies of Uncv gene.

Objective To study the images of the hyperopia using the twinkling image screening analyzer,and improve the referred ratio of the amblyopia.Methods The amblyopic factors were screened in 29 children(51 eyes) from January 2009 to November 2010 by twinkling image screening analyzer,the binary linear correlation analysis was used to analyze the relationship between the diameter ratio of the crescent /pupil and the degree of the ametropia.Moreover,the images of the esotropia were analyzed.Results The binary linear correlation analysis showed the relationship between the ratio of the crescent /pupil and the degree of the ametropia could be expressed by the equation:the degree of the ametropia =-0.197 + 9.095 × crescent /pupil.The images showed esotropia in 11 cases,and the images had many types.Conclusion At the same size of the pupil,the degree of the ametropia can be concluded by the size of the crescent,and considered the age of the child can improve the referred ratio of the amblyopia.

Objective To synthesize the gene including the CDV-N protein antigen epitopes,and construct the baculovirus expression vector.Methods The N protein antigen epitopes was predicted by bioinformatics software and synthesized tandem.The fragment was cloned into the baculovirus expression vector pFastBac-Hta.Results The gene including the CDV-N protein antigen epitopes was obtained and successfully constructed the pFastBac-Nsynthesis expression vector.Conclusion The experiment laid the foundation for the CDV-N protein antigen epitopes expression in baculovirus.

Objective To obtain the IFN-γgene of Indonesian Cynomolgus monkeys and provide a theoretical and material basis for the production of the recombinant IFN-γ. Method Based on the published nucleotide sequence of IFNγ gene of rhesus monkey in GenBank,a pair of RT-PCR primers were designed and synthesized. Total RNA,isolated from the peripheral blood mononuclear cells by Trizol,was used as template to amplify the IFN-γgene by RT-PCR. The gene was identified by endonuclease,PCR and DNA sequencing. Result A 498 bp DNA fragments were amplified. DNA sequencing confirmed that the fragment was IFN-γof Cynomolgus monkeys. Compared with rhesus monkeys,humans and baboons,the homology of nucleotide sequence of IFN-γgene were 100% ,96 % and 99 % respectively. Conclusion The IFN-γ gene of cynomolgus monkey was identical to that of rhesus monkey.

Objective: To observe the effects of the AMBLYOPIA NO.1 on form-deprived amblyopic cats. Methods: Juvenile cats were divided into normal group, sutured group, control group, low-dose and high-dose group. The N75, P100,N75-135 of the pattern visual evoked potential (PVEP)on the left eyes were observed in groups. The visual acuities got from the sweep visual evoked potential (SVEP)were compared among the groups. Results: Regular waves were observed in the normal group. There were no significant waves seen in sutured group. The P100 and N75-135 were delayed in control and low-dose group, though recovered to some extend. The P100 and N75-135 recorded from the high-dose group attained the normal level and the waves were similar to those in the normal group. The SVEP visual acuities in sutured and control group were worse than that of the normal group. However, the SVEP visual acuities in low-dose and high-dose group recovered to normal level. Conclusion: The AMBLYOPIA NO.1 had the therapeutic effects on the form-deprived amblyopic cats.

Objective To compare the pattern visual evoked potential and the visual acuity got from the sweep visual evoked potential among the cats from normal, model and control groups. To study the visual electro-physiology of each group and to determine if suturing the eyelid of one eye for one week can generate a form-deprived amblyopic cat model. MethodsThe wave form, latency, total time to finish one trial and the vision acuity were analysed among the three groups. ResultsThe normal PVEP had two peaks forming “M type (the positive fluctuation upwards). The model group and control group had different waves, broken into several waves. In addition, the time to finish a trial was delayed. The N75 evoked by the sutured eye was prolongated. The SVEP acuity was worse than that of normal group and the difference was significant. The acuity got from the non-sutured eye in the control group revealed a normal level while the acuity of sutured eye remained poor and the difference was also significant. ConclusionSuturing one eyelid for one week can successfully establish a form-deprived amblyopic cat model.

目的观察左旋多巴对难治性屈光参差性弱视色觉、视力的治疗效果,并探讨其作用机理.方法30例6～17岁的难治性屈光参差性弱视患儿服用思利巴,观察服药前、服药后第1、3月及停药后1个月时的FM-100色调排序的总错误分值与轴向和视力.结果①FM-100色调排序:总错误分值在服药前明显高于正常同龄人,在服药后第1月时有显著性减少,可达到正常同龄人水平,并保持到停药后1个月时;排序的错误轴向主要在黄-蓝区域;总错误分值与视力无关.②视力:服药第1月时视力有显著性提高;服药第3月及停药后1个月时视力没有再度提高.结论弱视患儿的色觉异常,主要表现在黄-蓝区域且与视力无关;左旋多巴能显著改善弱视患儿的色觉,并对提高难治性屈光参差性弱视患儿的视力有一定作用.