Objective Study the effect of different preparation methods for SanHuangXieXinTang on the con-tent of free anthraquinone. Method HPLC method was developed to simultaneously quantify free anthraquinones in three different decotions(traditional decoction,granule decoction,jinpao decotion). Result The difference among the contents of free anthraquinone by different preparation methods was significant,the contents of free anthraquinone were orderly re-duced as follow:traditional decoction,granule decoction,jinpao decoction,except Rhein;the contents of Rhein were as follow:granule decoction,traditional decoction,jinpao decoction. Conclusion Different preparation methods for San-HuangXieXinTang had significant effect on the content of free anthraquinone.

This study was aimed to establish a HPCE method for the content determination of hesperidin and phillyrin in Bao-He-W an (BHW). Fused silica capillary (75 cm í 50 μm) was employed and 30 mmol·L-1 borax so-lution (8% acetonitrile, pH9.64) was served as the running buffer. Other conditions were as follows: electrokinetic injection was 50 kPa í 20 s; analytical voltage was 20 kV; temperature was 20℃; and detection wavelength was 254 nm. The silica capillary was flushed with 0.1 mol·L-1 sodium hydrate and the running buffer for 10 min before each injection, respectively. The results showed that the linearity of hesperidin was in the range of 0.10~2.40 mg·mL-1 (r=0.999 4), the average recovery was 99.85% and RSD=2.34%. The phillyrin was in the range of 0.07~0.84 mg·mL-1 (r=0.999 2), the average recovery was 99.16% and RSD=2.78%. It was concluded that the method was rapid and sensitive. It can be used for the quality control of content determination of hesperidin and phillyrin in BHW.

Objective:To screen the effective parts from Erchen Tang for expectorant and antitussive.Method:The mice were allotted into 12 groups as following: physiogical saline,DHS group,the five part from Erchen decocotion groups with high dose(HD) and low dose(LD,15,3.75 g·kg-1),the mice were orally administered for 3 days,the antitussive effect was observed by concentrated ammonia method after 1 h.The mice were allotted into 12 groups as following: physiogical saline,AHOS group,the five part fron Erchen decocotion groups with high dose(HD) and low dose(LD,15,3.75 g·kg-1),the mice were orally administered for 3 days,the expectorant effect was observed by increased the secretion of expect oration method after 30 min.The cavies were allotted into 12 groups as following: physiogical saline aminophylline group,the five part from Erchen decoction groups with high dose(HD) and low dose(LD,8.32,2.08 g·kg-1),the cavies were orally administered for 3 days,the antiasthmatic effect was observed by spray method after 1 h.Result:Essential oil,butanol and water fractions could reduce the frequency of cough,and increase secretion of expectoration,and inhibitory asthma(P<0.01,P<0.05);petroleum ether and ethyl acetate fractions could not reduce the frequency of cough,could not increase secretion of expectoration,and inhibitory asthma.Conclusion:The results show that petroleum ether and ethyl acetate fractions were invalid,essential oil,butanol and water fractions were the effective parts of Erchen Tang for expectorant and antitussive.

Aim To compare the content of berberine hydrochloride in hejian decoction(the traditional Chinese herbs mixed together then boiled with water)of XieXin Tang and the fenjian decoction(the single traditional Chinese herb boiled with water separately,before the Abstracts mixed)of XieXin Tang(composed of Scutellaria baicalensis,Rheum palmatum and Coptis chinensis)and to compare their anti-bacterial activities in vitro.Methods A HPLC method was established with a Diamonsil-C18 column(4.6 mm× 250 mm,5 μm),a mobile phase of acetonitrile-0.05 mol·L-1sodium dihydrogen phosphate solution(adjust pH to 3 with phosphoric acid)(30∶ 70),a flow rate of 1 mL·min-1 and a detection wavelength of 350 nm in order to determine the contents of berberine hydrochloride.Minimal bacterial inhibitory concentration(MIC)and minimal bactericidal concentration(MBC)antagonizing the common bacteria in different decoctions were measured in vitro by dilution method.Results The average content of berberine hydrochloride of the fenjian decoction was higher than that of the hejian decoction.The hejian decoctions could display the inhibitory bactericidal activity to A.niger was stronger than that of the fenjian decoction.And to proteus,the inhibitory bactericidal activity of the fenjian decoction was stronger than that of the hejian decoction.The hejian decoctions and the hejian decoctions could display the inhibitory bactericidal activity to Staphylococcus aureus,S.albus,β-hemolyticstreptococcus.Conclusion Compared with that of the hejian decoction,the content of berberine hydrochloride of the fenjian decoction was higher,to A.niger,the inhibitory bactericidal activity of the hejian decoctions was stronger than the fenjian decoction,but to colon bacillus,the inhibitory bactericidal activity of the fenjian decoctions was stronger than the hejian decoction.

Objective:To study the compatibility of the ErChen decoction;Methods:Ethanol extract test solutions of the different combinations were prepared according to the uniform design of experiments with mixtures(U114),pharmacologic experiments,such as arresting cough,resolving phlegm.the diuretic effect were carried out with relationed grade analisis and quadratic stepwise regression analisis were applied to interrelationship between the amount of each drug and the pharmacologic data;Results:The results confirmed Rhizoma pinelliae and Pericarpium citri reticulatae are the basis medicines of the decoction,while Poria and Licorice are the adjuvant of it;Conclusion:This study provided a significant try of studying the compatibility of composite herbal medicines.

Objective To investigate the contents of Rhein,Emodin,Chrysophanol,Physcion in hejian decoction and the fenjian decoction of XieXin Tang and to compare with their anti-bacterial activities in vitro. Methods HPLC method was used to determine the componets of preparations.Minimal bacterial inhibitory concentration(MIC) and minimal bactericidal concentration(MBC) antagonized the common bacteria in different decoctions were measured in vitro by employing dilution method.Results The average content of componets in the hejian decoction was higher than that of the fenjian decoction.The hejian decoctions could display the inhibitory bactericidal activity to A.niger was stronger than that of the fenjian decoction. Conclusion Comparing with that of the fenjian decoction,the content of componets of the hejian decoction was higher to A.niger,the inhibitory bactericidal activity the hejian decoctions was stronger than the fenjian decoction.Against to Colon bacillus in the fenjian decoctions was stronger than that of in the hejian decoction.

Objective To determine the content of gallic acid and evaluate the quality of Galla chinensis of different habitats in Guizhou Province. Methods HPLC method was used to determine the content of Gallic acid on Alltima C18 column (4.6 mm×250 mm, 5 μm) with the mobile phase of methanol-water-phosphoric acid (15∶85∶0.085). The flow rate was 1.0 mL/min and the detective wave length was 270 nm. Results The contents of Gallic acid in Galla chinensis of 10 different habitats in Guizhou Province were 51.43%～ 60.25%. Conclusion The quality of Galla Chinensis in different habitats in Guizhou Province are up to the Chinese Pharmacopoeia, and the individual varibility is significant.

目的 建立同时测定戊己丸中盐酸小檗碱、芍药苷的高效毛细管电泳法,考察影响分离的各因素,确定最佳测定条件.方法 测定缓冲液为50 mmol/L的硼砂溶液:甲醇=1:1.5;未涂层石英毛细管(50 μm×64.5 cm,有效长度57.1 cm)为分离通道;操作电压30 kV;柱温25℃;检测波长230 mn;压力进样1.5 kPa×8 s.结果 盐酸小檗碱和芍药苷分别在0.037～0.460 mg/mL、0.037～0.463 mg/mL范围呈良好的线性关系,其平均回收率分别为99.22%和99.60%.结论 建立了快速、准确测定戊己丸中盐酸小檗碱、芍药苷含量的新方法 .

目的:建立酒龙胆饮片的HPLC指纹图谱鉴定方法。方法:采用RP-HPLC法,梯度洗脱,研究酒龙胆饮片的指纹图谱,并作相似度比较分析。结果:初步建立了酒龙胆饮片的指纹图谱。结论:采用HPLC指纹图谱可作为酒龙胆饮片的一项质控指标。

Objective To determine the content of gentiopicroside and evaluate the qulity of Gentiana rigescens from different habitats in guizhou province. Methods HPLC was used with Alltima C18(4.6 mm×250 mm,5 μm),methanol-water(30:70) as mobile phase,detector wavelength at 270 nm and column temperature at 30 ℃.The optimal extraction method for gentiopicroside was sdudied by orthogonal experiments. Results The calibration curve was linear with gentiopicroside in the range of 0.08～0.64 mg·mL-1(r=0.999 9),the average recovery was 102.4% with RSD of 1.69%(n= 5).The contents of gentiopicroside in Gentiana rigescens from 11 habits were between 2.113%～4.808%. Conclusion The quality of Gentiana rigescens from different habitats of guizhou province are consistent with the chinese Pharmacopoeia 2005,and there is no notable difference among them.

OBJECTIVE To determine the content of gentiopicroside.To evaluate the quality of Gentiana rigescens and its herbs pieces of different habitats in Guizhou Province.METHODS The extract methods of gentiopicroside was sdudied by orthogonal experiments.RESULTS The contents of gentiopicroside in Gentiana rigescens and its herbs pieces of 11 different habitats in guizhou Province were determined.CONCLUSION The quality of Gentiana rigescens and its herbs pieces in different habitats in Guizhou Province are up to the Chinese Pharmacopoeia,and the individual varibility is significant.

<正>戊己丸收载于《中国药典》2005年版一部,是由黄连、吴茱萸和白芍3味药细粉制成的水泛丸,功效主要为泄肝和胃,降逆止呕。其含量测定仅用紫外-可见分光光度法测定盐酸小檗碱的含量。除盐酸小檗碱外,吴茱萸碱和吴茱萸次碱也是戊己丸中重要有效成分,具有较强的药理作用,药典未收载其含量测定方法。经查阅文献资料,吴茱萸及其

OBJECTIVE To study the fingerprint of raw material of Eucommia ulmoides Oliv.from various habitats by HPLC.METHODS XDB-C18 column(250mm×4.6mm,5μm)was used,with mixtures of acetonitrile and 1% acetic acid as mobile phase in a gradient mode.The flow rate was 1mL·min-1 and detection wavelength was 277nm.RESULTS According to the optimum chromatographic conditions,good fingerprint of Eucommia ulmoides Oliv.from different habitats had been described.CONCLUSION The method was simple and accurate with good reproducibility.It provides a scientific basis for controlling the quality of Eucommia ulmoides Oliv..

目的:建立二陈汤中甘草次酸的含量测定方法.方法:采用高效毛细管电泳法.缓冲溶液由30mmol/L硼砂溶液和0.1mol/L碳酸钠(Na2CO3)溶液组成(加乙腈使其浓度至10%),pH=9.6.涂层石英毛细管50μm×95cm(有效长度86.8cm),在18kV电压下运行,50mpa压力进样20s,检测波长为254nm,温度20℃.结果:甘草次酸浓度在11-880μg/ml范围内呈良好线性关系(r=0.9998),平均回收率为101.27%,RSD为2.53%(n=6).结论;该方法简便、灵敏、准确.

目的:探讨药材配伍对陈皮中橙皮甙和总黄酮溶出率的影响,为系统研究二陈汤提供部分实验数据.方法:以橙皮甙、总黄酮为检测指标,测定它们在陈皮、陈皮与半夏、茯苓、炙甘草、生姜、乌梅等不同药材配伍组水煎液中的含量.结果:两两水煎液中橙皮甙含量差异均极显著(P＜0.01);总黄酮含量,陈皮加生姜与陈皮加乌梅组比较无显著差异,陈皮加乌梅组与全方水煎液比较无显著差异,其余两两水煎液之间差异极显著(P＜0.01或P＜0.05).结论:不同药材配伍对二陈汤中橙皮甙和总黄酮溶出率产生的影响不同.

目的:研究方药配伍对大承气汤,小承气汤和调胃承气汤君药大黄中有效成分蒽醌类衍生物溶出率的影响.方法:用薄层色谱-紫外分光光度法分别测定大黄水煎液及3方的分煎液与合煎液中游离大黄酸,游离大黄素,游离总蒽醌,结合型大黄酸,结合型大黄素,结合型总蒽醌的含量.结果:与大黄水煎液相比,大多数水煎液中游离蒽醌及结合型蒽醌含量存在显著性差异.合煎方中无论是结合型大黄酸还是结合型总蒽醌的含量均按如下顺序减少:大承气汤>小承气汤>调胃承气汤.结论:大黄与不同剂量的不同药材配伍共煎是使大,小,调胃承气汤中游离蒽醌,结合型蒽醌含量产生变化的重要原因.三承气汤中结合型大黄酸,结合型总蒽醌含量变化顺序与其泻下作用一致.

目的：考察甘草蜜制的几个影响因素：加蜜量（A），烘制温度（B），烘制时间（C）。方法：用正交试验设计L9(34)安排试验，分别测定了各炮制品中的甘草次酸和黄酮类化合物的含量，并采用综合评分法对测定结果进行分析。结果：影响甘草炮制的主次因素是B>A>C，蜜制甘草的最佳工艺是A2B3C2 ，即加入25%的蜂蜜，闷润透心后，在60 ℃烘60 min。结论：优选得到的工艺简单可行。

目的:研究药味配伍对小承气汤中有效成分蒽醌类衍生物溶出率的影响.方法:用薄层层析-紫外分光光度法测定了大黄及大黄与不同药物配伍组水煎液中游离蒽醌及结合型蒽醌的含量.结果:测定数据经统计检验显示,与大黄水煎液相比合煎方中结合型大黄酸及游离大黄素含量变化不显著,其余各配伍组水煎液中游离蒽醌、结合型蒽醌含量变化均极显著(P<0.02,P<0.02,P<0.001).结论:各种配伍药材对不同的蒽醌类成分产生的影响不同.

目的：探讨蒲黄炮制与其有效成分总黄酮、多糖含量的关系。方法：分光光度法。结果：蒲黄及各炮制品中总黄酮含量按如下顺序减少：生蒲黄＞ 酒炒蒲黄＞ 醋炒蒲黄＞ １４０ ℃烘蒲黄＞ 炒蒲黄＞ １８０ ℃烘蒲黄＞ 焦蒲黄＞２２０ ℃烘蒲黄＞ 炭蒲黄，除酒炒蒲黄外，各炮制品中总黄酮含量与生蒲黄相比差异极显著；醋炒蒲黄、１４０ ℃烘蒲黄、炒蒲黄、１８０ ℃烘蒲黄和焦蒲黄中多糖含量与生蒲黄相比明显增加，炭蒲黄则显著降低，酒炒蒲黄和２２０ ℃烘蒲黄却无显著性差异。结论：炮制温度、辅料黄酒和米醋能使蒲黄中总黄酮和多糖含量产生变化。

目的：研究不同药物配伍对四妙勇安汤中绿原酸、异绿原酸溶出率的影响。方法：用ＴＬＣＵＶ 分光光度法测定了四妙勇安汤分煎液、四妙勇安汤合煎液、金银花水煎液，金银花与方中其余各单味药材配伍水煎液中绿原酸和异绿原酸的含量，并对测定结果作了统计检验。结果：各配伍组水煎液中绿原酸含量均较金银花水煎液显著降低（ Ｐ＜ ０ ．０１） ；与金银花水煎液中异绿原酸含量相比，全方合煎液中异绿原酸含量无显著性差异，而其余各配伍组水煎液中则显著降低（ Ｐ＜ ０ ．０１） 。结论：方药配伍是四妙勇安汤中绿原酸、异绿原酸溶出率发生变化的主要原因之一。