采用DPPH.清除法对枸杞花提取物的体外自由基清除作用进行研究。结果表明,枸杞花具有一定的抗氧化活性,枸杞花乙醇提取物对DPPH.清除率为16.29%,蒸馏水提取物对DPPH.清除率为37.70%。

以苦豆子的茎尖、茎、根、上胚轴、下胚轴、胚根为外植体,以MS附加不同激素配比的培养基为基质,进行了诱导苦豆子愈伤组织的适宜外植体及培养条件的筛选。确定出苦豆子诱导愈伤组织的最佳外植体为胚根,最佳培养基为MS,适宜的细胞生长素2,4-D浓度范围为1.2～2.0 g/L,适宜的6-BA浓度为0.16 g/L。

The experiment optimized CTAB method to extractLycium barbarum L.NO.1and ruthenicum Murr.laminas genomic DNA.The concentration and purity of DNA were detected by UV spectrophotometer,agarose gel electrophoresis,and conducted a PCR amplification.12(10 bp)random primers were From 101 oligonucleotide primers on these two Lycium L.DNA samples amplified analysis,and 111 RAPD bands was detected,61 among which were polymorphic,the percent of polymorphic band was 54.95%.The results showed that the similarity factor ofLycium barbarum L.NO.1andLycium ruthenicum Murr.was S=0.618,the heredity was away from D=0.382.Therefore,the genetic relationship of barbarum L.NO.1andLycium ruthenicum Murr. was far.

Leaves,stems and roots of Cynanchum komarovii Al.were used as test material,using a modified CTAB Ⅰ method,CTAB Ⅱ method and SDS method to compared and analyzed the extraction effect of genomic DNA of Cynanchum komarovii Al.The results showed that,by the method of CTABⅠall could extraction higher concentration of DNA,electrophoretic bands with complete and clear;CTABⅡ although the three parts could was made from DNA,bands was bright,but there were obvious smearing;SDS in the leaves to make DNA,but stems and roots of the DNA content of the proposed small electrophoretic bands was dark;three methods mentioned in the amplification of DNA the RAPD results with CTABⅠ was the best,CTABⅡ was the second,and SDS method was the worst;to different parts of Cynanchum komarovii Al.average purity,concentration and yield of DNA extracted from leaves was the highest,RAPD amplification was the best,followed by stem and root minimum.CTABⅠ was efficient DNA extraction methods of Cynanchum komarovii Al.,to different parts of Cynanchum komarovii Al.,the yield of DNA extracted from leaves was highest,amplified the best,that was easier to obtain high-quality leaf DNA.

1 植物名称 牛心朴子(Cynanchum komarovii ALIljinshi). 2 材料类别 无菌胚轴. 3 培养条件 种子萌发与生长培养基:(1)种子萌发培养基:MS培养基;(2)不定芽诱导培养基:MS+6-BA 2.0 mg/L+NAA 0.1 mg/L;(3)生根培养基:1/2 MS+IBA 0.5 mg/L.上述培养基均添加3%蔗糖和0.7%琼脂,pH 5.8～6.0.培养温度为23～26 ℃,光照时间为16 h/d,光照强度约34μmol/(m·s).