目的 考察发酵时间pH值变化与菌丝生长的关系,为放大生产积累数据.方法 种子罐规格:500L、2000L、15T;材质:304不锈钢;菌株:虫革菌种(PL-12);制备工艺:生物发酵.结果 菌丝体经缓慢生长期,快速生长期,稳定期pH值呈下降趋势,随着发酵时间延长,pH值回升,判定菌丝生长进入衰退期,终止发酵.结论 制备工艺稳定、可控,可得理想菌丝体.
目的 建立壮肾安神胶囊中淫羊藿苷的含量测定方法.方法 采用HPLC,色谱柱:DikmaTechnologies C18(Φ4.6×200mm,5μ);流动相:乙腈-水(30:70);流速:0.6ml/min;柱温:30℃;检测波长为270nm.结果:淫羊藿苷在0.0642～1.284μg(r=0.9998)范围内呈良好的线性关系;平均回收率为98.80％;RSD％=0.33％.结论 方法简便可行、易于操作、准确、可靠,适用于壮肾安神胶囊的质量控制.
Objective to establish a determination method for borneol in soft capsule of Chuansu heart rescue.Method: GC instrument making with 1102GC of Shanghai analytical company,GCD-300A automatic hydrogen generator and stationary phase of 10% polyethylene glycol adipic acid ester;taking temperature programming: 2min holding at 60℃ before raising temperature to 100℃ at rate of 5℃/min;theoretical plates number should not below 1900.Result: the good linear relationship has been shown in the range of 0.00832ug-0.208ug of borneol,r=0.9999;the average recovery is 100.7%,RSD=2.38%.Conclusion: the method is accuracy and reliable,with characteristics of simple and convenient,easy to operate,suitable to quality control for Soft Capsule of Chuansu Heart Rescue.
目的建立更年宁中黄芩苷的含量测定方法。方法采用RP-HPLC,色谱柱:DiamonsilC18(4.6mm×250mm,5μm);流动相:甲醇-0.1%磷酸溶液(48:52);检测波长:280nm;流速:0.8ml/min;柱温:40℃。结果黄芩苷在0.1024～0.9216μg(r=0.9999)范围内呈良好的线性关系,平均回收率为99.72%,RSD%=0.17%。结论方法简便可行、易于操作、准确、可靠,适用于更年宁的质量控制。
目的:制定天舒软胶囊质量标准.方法:将原天舒胶囊改变剂型制成软胶囊,用薄层色谱法对样品中的川芎、天麻进行定性鉴别.用高效液相色谱法测定天舒软胶囊中川芎主要成分阿魏酸的含量.结果:阿魏酸在0.0202μg～0.5454μg范围内具有良好的线性关系,r=1.0.结论:本质量标准能有效地控制天舒软胶囊的产品质量.
