The use of azide ions (mainly in the form of NaN3 solutions) as test reagents in chemical, photochemical, and chemiluminescent studies, as well as the development of a specific chemiluminescent reagent for singlet oxygen are considered.

Quantum yields are determined for luminol and Fe II in the chemiluminescence of luminol initiated by Fe II salt solutions. It is concluded that chemiluminescence is initiated not by superoxide anion but by a more efficient primary oxidant. The quenching of chemiluminescence under the action of dimethyl sulfoxide shows that the hydroxyl radical also cannot be a primary oxidant. It is established that the effect the chelation of Fe II by EDTA has on chemiluminescence is not related to loss of the initiating action of Fe II EDTA in the luminol chemiluminescence. Instead, it is associated with its quick transformation into Fe III EDTA when preparing a mixture of Fe II and EDTA-Na 2 .

n this experimental study heart rate variability (HRV) using electrocardiography was studied in a small group of volunteers (10 people) who have never smoked (4), ex-smokers (4) and active smokers (2). Different nicotine delivery systems and smoking simulators, including regular, nicotine-free and electronic cigarettes, tobacco heating systems, chewing gums and a completely new product on the domestic market – nicotine packs of oral fixation (NPOF) were used. Supplemented with hormonal studies (stress hormones in saliva and catecholamines in the blood), HRV analysis showed relatively small changes in HRV with systemic use of packs with moderate nicotine content (up to 6 mg), which makes this type of product a leader in biological safety among the studied alternative delivery systems nicotine.

1 Институт биохимической физики им.Н.М.Эмануэля РАН (Москва, Россия) 2 Первый Московский государственный медицинский университет им.И.М

Meso-tetra (4-carboxyphenyl) porphyrin complexes with nano-sized diamonds were studied spectroscopically. The polarization characteristics of their steady-state fluorescence indicate the formation of a covalently bound complex between porphyrin molecules and diamond nanoparticles. The hydrodynamic volumes of the studied complexes were estimated on the basis of data on steady-state fluorescence anisotropy. Measurements have shown that the photophysical properties of porphyrin do not change significantly when an organo-inorganic hybrid complex with nanodiamonds is formed. The studied complexes can be promising in the field of biomedical research, in particular, for diagnostic purposes and for the development of new generation photosensitizers for practical medicine.

AIM:To justify the concept of systemic membrane-destabilizing distress syndrome in surgery via analysis of phospholipid bilayer of cell membranes of various organs in urgent surgical abdominal diseases.MATERIAL AND METHODS:Experimental research on dogs (n=90) included modeling of peritonitis, pancreatitis, intestinal obstruction, obstructive jaundice, and post-hemorrhagic anemia. Clinical and laboratory studies were performed in patients (n=119) with acute peritonitis, severe pancreatitis, intestinal obstruction, post-hemorrhagic anemia, acute cholecystitis, gastrointestinal bleeding, benign mechanical jaundice. Lipid profile in tissues and blood cells was determined by extraction, fractionation and densitometry. Moreover, we assessed intensity of lipid peroxidation and phospholipase activity, endogenous intoxication, functional state of organs and blood cells.RESULTS:It was revealed that all above-mentioned acute abdominal diseases are followed by significant changes of lipid bilayer and dysfunction of tissues in target organs, blood cells and other organs (liver, kidney, colon and small intestine, heart, lungs, spleen, brain). Changes of phospholipid bilayer are correlated with severity and course of the disease. These data were used to determine a new complex in surgery - systemic membrane-destabilizing distress syndrome. Its concept, pathogenesis, and diagnosis are presented. It was analyzed its role in development and progression of dysregulation pathology and thanatogenesis. Evidence of its importance in the pathogenesis of surgical aggression was obtained.

Model studies on Coumarin 343–detonation nanodiamond complexes are described. The complexation of coumarin molecules with diamond nanoparticles due to covalent bonding leads to a considerable change in their spectral-luminescence properties, which makes it possible to distinguish between the behaviors of a free dye and a bound luminophore in the studied objects, in particular, in cell cultures. Experiments with an acute monocytic human leukemia culture show that the studied diamond nanocomplexes are easily captured by the cells and visualized in them; therefore, these complexes can be used to transport photocontrolled reagents and, ultimately, new drugs.

The article presents a review of fluorescent material use: inorganic phosphors, organic polymeric and molecular phosphors, complex (coordination) compounds, quantum dots and frame metal-organic compounds as component materials of fluorescent converters to transform radiation of blue and ultra-violet diode crystals to white light.

An experimental setup is proposed in this work that allows measurement of beat signals during the superposition of the related components of the orthogonal expansion describing the electric field of the total light wave. The results of experimental studies obtained in real time via optical heterodyning accompanied by the acousto-optic interaction of two light waves in the Bragg diffraction regime are presented. (C) 2017 Optical Society of America

We have studied the feasibility of using luminophores in the coumarin series in luminescent filters for correcting the spectra of light-emitting diode (LED) light sources. Fine tuning of the emission spectra of luminophores in polymer matrices is achieved by introducing nonionic surfactants into the matrix. We propose a method for estimating the photostability of luminescent filters and we determine the photostability parameters.

Significant metabolic alterations in the liver were observed in dogs with modeled acute peritonitis. These changes significantly impaired detoxification function of the liver, which was seen from the increase in the titer of toxic products in the early post-surgery period not only in the lymph, but also in the blood plasma. The key pathogenic mechanism leading to acute liver failure is destabilization of cell membrane resulting from LPO, phospholipase activity, and tissue hypoxia. Activation of LPO and increase in phospholipase activity in the liver tissues were observed within 12 h after peritonitis modeling.