Objective To investigate anti - inflammatory effect of indirubin and tryptanthrin on LPS Induced RAW264. 7 inflammatory cell model. Methods Murine macrophageRAW264. 7 cells were stimula-ted by 10 μg/ ml LPS for 24 h to establish an UC inflammatory cell model. Cell viability was detected by MTT assay to explore the safe range of concentrations. UC inflammatory cell model was treated by indirubin and tryptanthrin in two different ways:treatment mode and prevention mode. IL - 6 and TNF - αin supernatant was tested by Elisa assay. Results IL - 6 and TNF - α in supernatant in model group were significantly higher than control group(P ﹤ 0. 01);Under treatment mode,IL - 6(5. 0、10. 0 μmol/ L)and TNF - α(2. 5、5. 0、10. 0 μmol/ L)in supernatant were reduced by indirubin significantly( P ﹤ 0. 05);IL - 6(0. 8、8. 0μmol/ L)and TNF - α(0. 8 μmol/ L group)in supernatant were reduced by tryptanthrin significantly(P ﹤0. 01,P ﹤ 0. 05,P ﹤ 0. 01);While under prevention mode,only IL - 6(0. 08、0. 8 μmol/ L groups)in super-natant were reduced by tryptanthrin(P ﹤ 0. 05). Conclusion Indirubin and tryptanthrin could inhibit in-flammation in UC inflammatory cell model in vitro,mainly under treatment mode by down - regulating IL - 6 and TNF - αexpression.

目的 研究青黛对溃疡性结肠炎(UC)体内、体外炎症模型的抗炎作用.方法 大鼠随机分为正常组、模型组、青黛高剂量组、青黛低剂量组,以低浓度TNBS/乙醇溶液灌肠诱导大鼠实验性结肠炎体内炎症模型,连续以青黛高、低剂量混悬液灌胃治疗10 d,酶联免疫吸附法(ELISA)检测血清白细胞介素-l(IL-1)、IL-6、IL-8浓度.以脂多糖(LPS)刺激小鼠巨噬细胞株RAW264.7细胞24h诱导体外炎症模型,以MTT法检测青黛对细胞生存率的影响,以ELISA法研究青黛在直接干预、预防干预2种模式下对细胞培养液IL-6、肿瘤坏死因子-α(TNF-α)表达的影响.结果 动物实验:模型组大鼠血清中IL-1、IL-6、IL-8浓度均明显高于空白组(P＜0.05),青黛高剂量组血清中IL-1、IL-6、IL-8含量均明显低于模型组(P＜0.05),青黛低剂量组血清IL-6、IL-8浓度明显低于模型组(P＜0.05).细胞实验:模型组中IL-6、TNF-α水平较正常组均有明显升高(P＜0.05);青黛直接干预时,10 μmol/L青黛组细胞培养液IL-6、TNF-α均较模型组有明显下降(P＜0.05);青黛预防性干预时,10 μmol/L青黛组IL-6明显下降(P＜0.05).结论 青黛对溃疡性结肠炎有一定的体内、体外抗炎作用,抗炎机制可能为下调炎症因子的表达.

目的:探讨老年人高血压的临床特点,为临床治疗提供帮助.方法:回顾性分析126例60岁以上老年高血压患者(老年组)的临床资料,并与92例非老年高血压患者(非老年组)进行比较.结果:临床表现方面无症状和头晕头痛患者两组无显著差异,心悸气促者老年组与非老年组差异有统计学意义；老年组晨峰高血压现象明显,P＜0.01；老年组脉压差明显增大、单纯收缩期高血压多见,并发基础疾病多.联合用药老年组高于非老年组,差异有统计学意义(x2＝27.117,P＜0.01).结论:根据老年高血压的临床特点,遵循个体化原则,合理用药、平稳降压是治疗老年高血压的关键.

Objective To observe the curative effect of Puji hemorrhoids suppository on treating the hemorrhoids patients with constipation.Methods All 124 patients with hemorrhoids were divided into two groups,the treatment group and the control group,each group 62 cases.The treatment group was Puji hemorrhoids suppository,and the control group was treated Mayinglong musk hemorrhoids ointment.Results After 14days,the total effective rate of the treatment group was 92.0%,the control group was 87.1%.The improvement in constipation of treatment group were significantly more than the control group(P0.01).Conclusion Puji hemorrhoids suppository has an available,definite and reliable therapeutical effect for treating the hemorrhoids.Meanwhile Puji hemorrhoids suppository can cure the constipation of patients with hemorrhoids

目的:观察补肾通络治疗糖尿病骨质疏松症(DOP)的效果.方法:73例DOP患者随机分为治疗组37例和对照组36例.两组均予降糖治疗,治疗组以补肾通络中药治疗,对照组以复方碳酸钙片.结果:补肾通络中药能够明显改善患者的临床症状,而跟骨超声检测(quantitative ultrasound QUS ):声音速度(speed of sound,SOS)、宽波段超声衰减(broadband ultrasound attenuation,BUA)较治疗前有显著改善.结论:补肾通络治疗DOP确切疗效,值得进一步深入研究.

Objective To observe quantitative ultrasound(QUS) changes in elderly male patients with type 2 diabetes,and investigate the relationship with blood glucose level,HbA_(1c) and urinary albumin excretion(UAE). Methods There were 79 elderly male patients with type 2 diabetes in the study group,43 patients without diabetes in the control group.Patients with diabetes were divided into four subgroup:Group I:HbA_(1c)<7%,Group IA;UAE<30 mg/24 h,Group IB:UAE>30 mg/24 h,Group II:HbA_(1c).>7%,Group IIA:UAE<30 mg/24 h and Group IIB:UAE>30 mg/24 h.Glucose,HbA_(1c),ALP,Cr,UAE,BUA,SOS and STI were measured.Results Compared with control group,BUA,SOS and STI in diabetic group decreased significantly(P<0.01).BUA,SOS and STI in diabetes subgroup of IIB was significandy lower than in subgroup IIA,subgroup IA and group IB(P<0.05).Conclusion The BUA,SOS,STI are lower in diabetic group than in controls group.The BUA,SOS and STI of patients with abnormal blood glucose and microalbuminuria>30 mg/24 h are decreased more significantly than those with normal blood glucose and microalbuminuria<30 mg/24h.