脊髓损伤(SCI)是一种严重的中枢神经系统疾病,具有高发病率、高致残率、高耗费和青壮年多发等特点.据世界卫生组织调查显示,外伤性脊髓损伤的年发病率约为12.1～58.7/1000000/年[1],而且呈逐年上升趋势,每年大约有50万新增患者[2].SCI给患者个人及其家庭乃至整个社会带来了沉重的负担,其治疗至今仍是医学界的难题之一.在治疗SCI的药物中,甲基强的松龙(MP)在临床上使用最为广泛,但由于MP可增加患者胃肠道出血、肺部感染、高血糖血症等并发症的发生率,故MP的临床使用率呈现出动态下降的趋势[3].此外,神经营养因子、神经节苷脂、降解胶质瘢痕药物、氧自由基清除剂和抗氧化剂等药物也常常被用于SCI的治疗,但这些药物作用较单一,疗效不能令人满意.因此,寻找疗效显著且毒副作用小的药物来满足临床治疗SCI的需要是当今基础和临床研究的焦点和热点.

Diffuse alveolar hemorrhage (DAH) is a life threatening clinical syndrome caused by a variety of causes.Early identification and etiological diagnosis of DAH in children are challenging.Despite some advances have been made in the identification and management of DAH,the mortality rate is still high.This article aims to raise the cognition of clinicians on DAH by providing a general review of some recent researches.

Objective To investigate the subclinical infection status of enterovirus 71(EV71) and coxsackievirus A 16(CVA16)in health children from kindergarten in areas of Guangxi province(primarily in Baise, Duan and Pingguo areas)for evidence to prevent hand-foot-mouth disease(HFMD).Methods 135 aliquots of serum samples were collected from health children in kindergarten.ELISA was performed to detect IgM and IgG antibodies specific to EV71 and CVA16.Results Of the 135 samples, positive EV71 IgM, CVA16 IgM, EV71igG, CVA16 IgG were detected in in 3(2.22%), 12(8.89%), 110(81.48%), 112(82.96%), respectively.Positive rate of EV71 IgM and IgG as well as CVA16 IgM and IgG was 0.00% and 86.76%;0.00% and 86.76% for girls, 4.48% and 76.12%;4.48% and 76.12% for boys, respectively.The difference was not significant in genders(P>0.05).Conclusion Subclinical infection of EV71 and CVA16 are very common in younger children, suggesting that they are highly risk group.

Objective The aim of this study was to investigate the value of detection of serum antibody of EV71-IgM in early diagnosis for hand foot and mouth disease (HFMD) by EV71 infection.Methods A total of 436 cases of children clinically diagnosed with HFMD from April to October 2014 and April to October 2016 in Guangxi were studied.All the serums were tested for EV71-IgM.Throat swabs or anal swabs were collected from 136 cases of 436 children to detect viral nucleic acid by fluorescence quantitative RT-PCR.All data were analyzed with SPSS 17.0.Results Among 436 cases of clinical diagnosed HFMD,256 cases were EV71-IgM antibody positive,while 180 cases were negative.The positive rate of EV71-IgM antibody in fatal group and severe group was significantly higher than that in mild group (x2=88.016,P＜0.001).The fluorescent quantitative RT-PCR was used as the standard.In 136 cases,comparing the EV71-RNA detected from throat swabs or anal swabs with EV-IgM detected from serum,the detection consistency of the two methods was moderately reliable (Kappa=0.575,P＜0.001),the sensitivity of EV71-IgM detection was 93.67％(74 /79),the specificity was 61.40％ (35/57).The positive rate of EV71-IgM antibody was gradually increased in the course of 1-3 days after the onset.The detection rate was 92.31％ in the third days,and the detection rate was 100％ in the course of 4-10 days after the onset.Conclusion Rapid detection of serum EV71-IgM antibody had good application value,providing a high sensitivity and convenient operation way for early diagnosis of EV71 infection.

Objective:To investigate the etiological characteristics of 319 cases of hand foot mouth disease (HFMD).Methods:A total of 319 children diagnosed with HFMD from January 2014 to January 2016 in Guangxi were collected and divided into three groups:fatal group (85 9ases),severe group (132 cases) and mild group (102 cases).Serum EV71-IgM antibody and CVA16-IgM antibody were detected with enzymelinked immunosorbent assay (ELISA).Results:The positive rate of EV71-IgM antibody in the fatal group and severe group were significantly higher than that in the mild group (P ＜0.05).The positive rate of EV71-IgM antibody in death cases was 85.71 ％.The positive rate of CVA16-IgM antibody in mild group was significantly higher than those in the fatal group and severe group (P ＜0.05).There was no significant difference on the gender ratio and age composition among EV71-IgM antibody positive cases,CVA16-IgM antibody positive cases,EV71-IgM antibody and CVA16-IgM antibody double positive cases,EV71-IgM antibody and CVA16-IgM antibody double negative cases (P ＞0.05).Conclusion:The etiology analysis revealed that severe and fatal HFMD were mainly caused by EV71.Meanwhile,we need to pay attention to the possibility that CVA16 and other enteroviruses infected cases developing into severe ones.

感冒是较常见的疾病,目前,治疗感冒的药物种类较多且剂型各不相同.对不同剂型的感冒药物进行初步的探讨、分析、阐述、说明,引导感冒患者科学合理地使用感冒药物,从而增强感冒药物的实际疗效,让患者早日走向康复.

为了探讨黑茶抗衰老的活性物质及其作用机制,本文收集了近10年黑茶及抗衰老的相关文献进行整理和分析,总结出了黑茶抗衰老的活性物质为茶多酚,其作用机制主要与茶多酚抗氧化、调整血脂水平、抗辐射以及影响基因表达作用密切相关.此研究旨在为进一步开发黑茶功能保健品提供参考.

目的:探讨鸡骨草胶囊中主要原料药栀子的含量测定鸡骨草胶囊方法及提取工艺.方法:采用高效液相色谱法测定鸡骨草胶囊有效成分含量,应用正交设计法优选栀子提取工艺.结果:栀子苷在0.06～1.2μg(r=0.999 8)范围内线性关系良好,平均加样回收率为101.28％(RSD=1.18).结论:该方法简便、有效、准确、重复性好,可用于鸡骨草胶囊有效成分的提取和测定.

Objective To study the protective effect of Equisetum ramosissimum extracted with percolation method on Carbon terachoride (CCl 4 ) induced acute liver injuryed mice. Methods Mice were randomly divided into six groups:normal control group,acute liver injury model group,bifendate control group,high,middle,low dose group. Intragastric administration every day for contious 10 days. After the last administration,groups were peritoneal injection with 0.08% CCl 4 -0.1 mL/10 g peanut oil in addition to normal control group.All the mice were sacrificed to assay antioxidant activities in the blood at 16 hours. Results The contects of serum GOT,GPT activities revealed an obvious decrease in the Equisetum ramosissimum group when compared to the model group,bifendate control group and equisetum ramosissimun of high,middle,low groups showed noticeable effects. Conclusion Equisetum ramosissimum can protect against CCl 4-induced acute liver injury in mice.