ObjectiveTo systematically investigate the impact of adjusting the relative weight of the built-in Stereotactic Radiosurgery Normal Tissue Objective (SRS-NTO) on dosimetric quality, plan complexity, and delivery efficiency in HyperArc™ stereotactic radiosurgery (SRS) for brain oligometastases.MethodsIn this retrospective planning study, a cohort of 20 patients with 1-3 brain oligometastases was analyzed. For each case, six distinct HyperArc plans were designed and optimized using the Varian Eclipse™ Treatment Planning System. To precisely isolate its impact, the relative weight of the SRS-NTO to the PTV objective was systematically varied across six levels-50%, 75%, 100% (default), 125%, 150%, and 200%-while all other planning parameters were held constant. A comprehensive comparative evaluation was then performed to assess the plans across four key domains: (i) dosimetric quality, evaluated by metrics including the Paddick Conformity Index (CI), Gradient Index (GI), and dose to Organs at Risk (OARs); (ii) plan complexity, characterized by various modulation and aperture-based indices; (iii) delivery efficiency, primarily quantified by the total Monitor Units (MUs); and (iv) physical deliverability, verified via Gamma analysis.ResultsIncreasing NTO weight did not significantly alter dosimetric quality; key metrics for CI, GI, and OAR sparing remained statistically equivalent (p > .05). Conversely, higher NTO weights prompted a significant reduction in total MUs (p < .001) that reached an optimum at the 150% setting, and enhanced plan deliverability, evidenced by significantly higher Gamma passing rates under stricter verification criteria. An inflection point was observed beyond the 150% setting, with higher weights leading to degraded plan complexity and efficiency. Strategies within the 125% to 150% range demonstrated a superior balance, achieving optimal dosimetric trends while maximizing gains in efficiency and precision.ConclusionIn HyperArc SRS for brain oligometastases, moderately increasing the SRS-NTO weight from the default 100% into the 125% to 150% range is a superior clinical strategy. This adjustment significantly enhances treatment efficiency and delivery precision by reducing plan complexity, without compromising dosimetric quality, thereby achieving a superior overall performance.

Antibiotic-resistant bacteria produce some virulence factors as immune evasion molecules that interfere with a variety of innate immune defenses, which is associated with reduced levels of neutrophil recruitment. Antibiotics and neutrophils recruited by endogenous chemokines are not able to efficiently eliminate the bacteria at the site of infection. Here, a nano-chemoattractant (fZnCB) is developed with photodynamic therapy and neutrophil recruitment by a multi-component self-assembly strategy to coordinate benzoxaborole-modified Chlorin e6 (Ce6-BN) and formylated peptides (fMLFH) through Zn2+. This innovative system synergistically collaborates with neutrophils through photodynamic effects to combat drug-resistant bacteria. Furthermore, the nano-chemoattractant not only demonstrates exceptional bacterial adhesion properties, biofilm dispersion by photodynamic activity but also boosts the recruitment, engulfment, and killing of bacteria by neutrophils. Its remarkable therapeutic efficacy against bacterial keratitis has been validated in a mouse model. This offers a new insight into an alternate approach for treating infectious diseases caused by drug-resistant bacteria.

目的:研究颅内肿瘤放疗中kV级锥形束CT(cone-beam CT,CBCT)引导引入的眼晶体吸收剂量,为颅内肿瘤放疗中计算眼晶体的额外吸收剂量提供数据支持.方法:选取某院2014年6月至2016年5月的30例颅内肿瘤放疗病例,将30例病例的计划CT数据导入EGSnrc程序进行Monte Carlo模拟,分析高质量头、标准剂量头、低剂量头和头部4种扫描协议5个等中心位置下的CBCT引导引入的眼晶体吸收剂量.采用SPSS 26.0进行统计学分析.结果:同一扫描协议不同等中心的眼晶体吸收剂量结果显示,除位于眼晶体后方的等中心的吸收剂量与其他4个等中心差异有统计学意义外(P<0.001),其余4个等中心间差异均无统计学意义(P>0.05);每个等中心不同扫描协议下的眼晶体吸收剂量差异均有统计学意义(P<0.001).结论:不同的扫描协议、不同的等中心位置对CBCT引导引入的眼晶体吸收剂量均有影响,前者大于后者;CBCT引导引入的眼晶体吸收剂量不容忽视,引导次数较多时应在治疗计划中充分考虑其影响.

Infectious keratitis is a serious disease originating when corneal trauma is infected with bacteria and has difficulties in healing due to stubborn infection and persistent inflammation featuring high reactive oxygen species (ROS) concentrations. Herein, a synergistic antibacterial and wound-healing strategy is proposed to treat bacterial keratitis by using poly(phenylboronic acid-(3,4-dihydropyrimidin-2(1H)-one))-co-(2-lactobionamidoethyl methacrylate) (p(PBA-DHPM-r-LAMA)) glycopolymeric micelles. The phenylboronic acid-(3,4-dihydropyrimidin-2(1H)-one) (PBA-DHPM) groups not only act as active targeting ligands to allow epithelial penetration and bacterial anchorage, but also perform as effective ROS-scavenging agents to relieve inflammation. By co-encapsulating levofloxacin (LEV) and chondroitin sulfate (CS) into the antioxidant glycopolymeric micelles, the resulting nanoplatform can achieve multiple functions of bacterial killing, ROS scavenging and promoting wound healing. Regarding the positive control, the nanoplatform demonstrated reasonable and comparable free radical scavenging potential in terms of DPPH, ABTS and hydroxyl radical scavenging. In addition, the glycopolymeric nanoplatform exhibited enhanced antibacterial activity compared to free LEV benefiting from the promoted epithelial penetration and the bacteria-activated drug release pattern. In vivo treatment of Staphylococcus aureus (S. aureus)-induced bacterial keratitis on a rat model showed that the drug-loaded nanoplatform could effectively cure bacterial keratitis within 5 days. Overall, this work provides an effective antibacterial and wound-healing strategy based on a ROS-scavenging polymeric nanoplatform for treating bacterial keratitis.

Background: To study the feasibility of kilovoltage cone-beam computed tomography (KV-CBCT) dose calculation following scatter correction. Methods: CIRS 062 and Catphan 504 phantoms were used in this study, and 40 randomly selected subjects representing a variety of cases (ten head cancer cases, ten chest cancer cases, ten abdominal cancer cases and ten pelvic cavity cancer cases) were enrolled. We developed in-house software called the cone-beam CT imaging toolkit (CITK) to improve the quality of CBCT images. We first aligned each planning computed tomography (pCT) image with the corresponding CBCT image using rigid registration after scatter correction. Hounsfield unit-relative electron density (HU-RED) calibration was applied to the CBCT images. The pCT plan was then recalculated on CBCT images. Finally, the dosimetric differences between the two plans were evaluated. The dosimetric parameters included the D98, D2, Dmean, conformity index (CI), homogeneity index (HI) and other organ at risk (OAR) dose parameters of the planning target volume (PTV). The dose distribution index (DDI) and the gamma index were also assessed. Paired Student's t-tests or Wilcoxon rank tests were used to evaluate differences. P<0.05 was considered significant. Results: In the phantom and patient cases, the average dosimetric difference was less than 1% in the PTV and OARs. There was no significant difference in the CI or HI between the two plans. The gamma pass rate of 2%/2 mm was greater than 95% in both plans. There was a significant difference in the DDI between the two plans in the chest group but not in the other groups. Conclusions: The results suggest that CBCT has high accuracy in dose calculation via scatter correction and HU-RED calibration.

Chronic infections caused by Pseudomonas aeruginosa pose severe threats to human health. Traditional antibiotic therapy has lost its total supremacy in this battle. Here, nanoplatforms activated by the clinical microenvironment are developed to treat P. aeruginosa infection on the basis of dynamic borate ester bonds. In this design, the nanoplatforms expose targeted groups for bacterial capture after activation by an acidic infection microenvironment, resulting in directional transport delivery of the payload to bacteria. Subsequently, the production of hyperpyrexia and reactive oxygen species enhances antibacterial efficacy without systemic toxicity. Such a formulation with a diameter less than 200 nm can eliminate biofilm up to 75%, downregulate the level of cytokines, and finally promote lung repair. Collectively, the biomimetic design with phototherapy killing capability has the potential to be an alternative strategy against chronic infections caused by P. aeruginosa.

BACKGROUND AND OBJECTIVE:We aimed to further study the role of Myelin Transcription Factor 1(MyT1) in tumor and other diseases and epigenetic regulation, and better understand the regulatory mechanism of MyT1.METHODS:Using bioinformatics analysis, the structure and function of MyT1sequence were predicted and analyzed using bioinformatics analysis, and providing a theoretical basis for further experimental verification and understanding the regulatory mechanism of MyT1. The first, second and third-level structures of MyT1 were predicted and analyzed by bioinformatics analysis tools.RESULTS:MyT1 is found to be an unstable hydrophilic protein, rather than a secretory protein, with no signal peptide or trans-membrane domain; total amino acids located on the surface of the cell membrane. It contains seven zinc finger domains structurally. At sub-cellular level, MyT1 is localized in the nucleus. The phosphorylation site mainly exists in serine, and its secondary structure is mainly composed of random coils and alpha helices; the three-dimensional structure is analyzed by modeling.CONCLUSIONS:In this study, the structure and function of MyT1 protein were predicted, thereby providing a basis for subsequent expression analysis and functional research; it laid the foundation for further investigation of the molecular mechanism involved in the development of diseases.

[目的]探讨异丙酚联合脐带间充质干细胞上清液冻干粉对部分肝切除术(partial hepatectomy,PHT)大鼠血管再生及肿瘤坏死因子-α(tumor necrosis factor-alpha,TNF-α)、C-反应蛋白(C-reactive protein,CRP)、血管内皮生长因子(vascular endothelial growth factor,VEGF)及中性粒细胞百分比的影响.[方法]将雄性Wistar大鼠按照随机数字法分为3组,每组12只,其中A组麻醉用异丙酚进行诱导和维持,打开胸腔暴露肝脏(假手术组);B组麻醉用异丙酚加生理盐水干预进行诱导和维持,行PHT;C组麻醉用异丙酚联合间充质干细胞上清液干预进行诱导和维持,行PHT.在术后5d将大鼠处死,取出再生的肝脏,称重并评估;收集大鼠血清,用于检测TNF-αα、CRP和VEGF的表达水平.[结果]与A组比较,B、C两组TNF-αα和VEGF水平显著升高;与B组比较,C组TNF-αα水平显著降低,而VEGF水平明显升高;与B组比较,C组CRP血浆水平和中性粒细胞百分比显著降低;与B组比较,C组平均肝再生率显著升高,差异均有统计学意义(P＜0.05).[结论]异丙酚联合脐带间充质干细胞上清液冻干粉可有效降低TNF-αα、CRP水平和显著上调VEGF的表达,促进残肝再生.

放射治疗中,多次锥形束CT(Cone-beam CT,CBCT)扫描的累积辐射剂量不容忽视,对CBCT不同版本扫描协议(V1.4、V1.5、V1.6)的辐射剂量分析,可为临床应用中合理使用CBCT提供数据支持.按照美国医学物理学会(AAPM)111工作组建议的辐射剂量测量方法(TG111)使用加长型模体(长度为45 cm)和0.6 cm3指形电离室对Varian CBCT系统不同版本扫描协议的辐射剂量进行测量,根据TG111推荐的规范,计算得到CBCT的加权辐射剂量TG111W和归一化辐射剂量nTG111W.结果显示:V1.5版本(两种设备)扫描协议的加权辐射剂量整体高于另两个版本(盆腔协议除外),最大值为V1.5版本的高质量头协议(30.06 mGy),最小值为V1.4版本的低剂量头协议(2.83 mGy);归一化辐射剂量差异均较小,最大差异在标准剂量头协议中(V1.4和V1.5(TrueBeam)的差异为1.33 mGy),最小差异在低剂量胸和盆腔协议中(V1.5(TrueBeam)和V1.6的差异均为0.01 mGy).结果提示,各软件版本扫描协议的辐射剂量与管电压和曝光量等参数密切相关(曝光量影响最大),为了保证患者的安全,临床使用中需要根据使用扫描协议的不同,合理规划CBCT扫描的频次.

A bio-inspired injectable hydrogel as a cell platform for real time glycemic regulation by mimicking the function of native islet β cells.

Objective To investigate the effects and molecular mechanisms of targeted knockdown of MyT1 on the migration, invasion and adhesion of human glioblastoma cells. Methods shRNA specifically targeting MyT1 gene was designed, and the packaged lentivirus was used to infect human glioblastoma U-118MG and U-87MG cells. The expression levels of MyT1 mRNA and protein in U-118MG and U-87MG cells were detected by qPCR and Western blot. The migration, invasion and adhesion of U-118MG and U-87MG cells were respectively detected by BrdU assay, cell would healing assay, Transwell assay and adhesion assay. The expression levels of genes related to cell adhesion and tumor metastasis were detected by qPCR. Results The lentivirus specifically targeting MyT1 gene was successfully packaged in HEK293T cells and then infected U-118MG and U-87MG cells. The expression levels of MyT1 mRNA and protein were significantly down-regulated (both P < 0.05), and the migration, invasion and adhesion abilities of cells were decreased (all P < 0.05). The expression level of cell adhesion-related genes decreased significantly, while the expression level of tumor metastasis-related genes increased significantly (both P < 0.05). Conclusion Targeted knockdown of MyT1 gene inhibits the migration, invasion and adhesion of human glioblastoma U-118MG and U-87MG cells, and the mechanism may be related to the regulation of cell adhesion- and tumor metastasis-related genes expression. MyT1 may be a potential target for the diagnosis and treatment of glioblastoma.

目的:探讨放射治疗千伏级图像引导系统各扫描协议产生的辐射剂量,分析图像引导过程中产生的额外辐射剂量可能存在的潜在风险.方法:基于EGSnrc程序准确建模千伏级图像引导系统(瓦里安公司OBI)的基础上,对图像引导系统各扫描协议的辐射剂量进行模拟,模体采用国际放射防护委员会(International Commission on Radiological Protection,ICRP)发布的成年男女标准模体(ICRP 100).2种模体的组织当量剂量根据ICRP 103号报告推荐的方法计算,最终得到千伏级图像引导系统各扫描协议下辐射剂量的有效剂量.结果:标准剂量头、低剂量胸、盆腔3种扫描协议下,单次图像引导男模体的组织当量剂量分别为0.85、3.26、4.51 mSv,女模体的组织当量剂量分别为1.14、3.80、6.57 mSv;3种扫描协议的有效剂量分别为0.48、4.25、6.44 mSv;单个组织的平均辐射剂量最大值出现在膀胱中(盆腔模式),其值达到了37.56 mGy.结论:多次图像引导产生的累积辐射剂量有潜在致癌风险,临床应用中需引起重视.

背景:3D打印技术可以根据需求制备出满足脊髓植入形状、大小和表面形态要求的生物支架.目的:观察3D打印胶原/壳聚糖支架对脊髓损伤大鼠神经功能恢复的影响.方法:将胶原和壳聚糖按2∶1的质量比混合,采用冷冻干燥法制备普通胶原/壳聚糖支架,采用3D打印机制备3D打印胶原/壳聚糖支架,分别测量两种支架的孔隙率和弹性模量,电镜观察支架形态.将神经干细胞分别与3D打印胶原/壳聚糖支架、普通胶原/壳聚糖支架共培养,进行扫描电镜观察与CCK-8检测.将40只雌性SD大鼠(由中国人民解放军医学科学院军事科学院提供)随机分成4组:假手术组、脊髓损伤组、普通胶原/壳聚糖支架组和3D打印胶原/壳聚糖支架组,后3组制作脊髓全横断损伤模型,普通胶原/壳聚糖支架组和3D打印胶原/壳聚糖支架组损伤处填充对应的支架材料,术后相应时间点进行后肢功能BBB评分、斜坡实验、神经电生理检测与磁共振平扫.实验方案经天津市神经创伤重点实验室伦理委员会批准.结果 与结论:①扫描电镜显示,3D打印胶原/壳聚糖支架具有互连的多孔结构,普通胶原/壳聚糖支架内部结构紊乱;②神经干细胞在3D打印胶原/壳聚糖支架表面生长良好,完全伸展,且3D打印胶原/壳聚糖支架表面神经干细胞的活性显著高于普通胶原/壳聚糖支架组(P＜0.05);③3D打印胶原/壳聚糖支架的孔隙率与弹性模量均高于普通胶原/壳聚糖支架组(P＜0.05);④3D打印胶原/壳聚糖支架组术后3-8周的BBB评分高于脊髓损伤组、普通胶原/壳聚糖支架组(P＜0.05),术后4,6,8周的斜坡实验角度大于脊髓损伤组、普通胶原/壳聚糖支架组(P＜0.05);⑤3D打印胶原/壳聚糖支架组术后8周的运动诱发电位振幅、体感诱发电位振幅大于脊髓损伤组与普通胶原/壳聚糖支架组(P＜0.05),运动诱发电位潜伏期、体感诱发电位潜伏期短于脊髓损伤组与普通胶原/壳聚糖支架组(P＜0.05);⑥磁共振平扫显示与脊髓损伤组及普通胶原/壳聚糖支架组比较,3D打印胶原/壳聚糖支架组损伤处具有较好的连续性与较多的神经纤维束通过;⑦结果表明,3D打印胶原/壳聚糖支架可促进脊髓损伤大鼠神经功能的修复.

直肠癌放射治疗中,由于摆位误差的存在,患者体内实际得到的剂量与计划设计相比总有不同程度的差异.选取20例直肠癌RapidArc病例,首先将pCT图像的治疗计划移植到散射校准后的锥形束CT(CBCT)图像上生成初始CBCT计划,随后该计划经每周的摆位误差数据修正等中心位置后进行剂量计算,生成修正后的CBCT计划,最后用配对t检验比较CBCT计划与pCT计划剂量计算结果的差异.结果显示:CBCT计划的剂量学参数结果与pCT计划相比,靶区D2、D98、D50、CI、HI的平均值均在一定程度上减小,差异依次为2.8％、2.5％、2.9％、2.0％、4.4％,均有统计学意义(p<0.001);在小肠中Dmean、D5、V20、V30和V40的平均值均有一定程度的增大,差异依次为3.0％、3.1％、2.5％、3.1％、3.6％,均有统计学意义(p<0.001);在膀胱中Dmean、D5、V20、V30和V40的平均值均在一定程度上增大,差异依次为3.0％、1.8％、0.9％、3.2％、3.4％,均有统计学意义(p<0.001);左右股骨头的差异较小(最大为0.3％,最小为0.1％),但结果均有统计学意义(p<0.001).实验结果提示:基于散射校准后的CBCT图像结合摆位误差数据制定的CBCT计划,可为直肠癌患者放疗中各组织实际吸收剂量提供参考,为临床使用中定量评估治疗效果提供数据支持.

目的:基于EGSnrc程序实现对锥形束CT系统的模拟仿真,并对所建立系统的准确性进行验证.方法:通过EGSnrc程序的BEAMnrc子程序将锥形束CT系统分为X射线源、射线源组件、探测器、Monte Carlo模拟用模体4个模块并对其进行建模.对所建立的模拟仿真系统的X射线能谱、百分深度剂量、离轴剂量与第三方软件或实测结果进行分析比较,以验证其准确性.结果:模拟仿真系统的能谱与Spektr程序生成的能谱有良好的一致性,最大差异为3.0％,平均差异小于2.0％;Monte Carlo模拟的百分深度剂量和横、纵向离轴剂量结果与实测结果的一致性良好,最大差异分别为2.7％、4.0％、2.7％,平均差异分别在±2.0％、±1.1％、±0.8％以内.结论:建立的模拟仿真系统可实现锥形束CT的准确模拟,可为研究锥形束CT的相关问题奠定基础.

Objective To investigate the supernatant of umbilical cord-derived mesenchymal stem cells (UCMSCs) on tumor necrosis factor-α(TNF-α) and apoptosis protein caspase-3 in diabetic rats model with skin ulcer. Methods 45 Sprague-Dawley rats were randomly divided into control group (acute wounds group), phosphate buffered saline (PBS) group and UCMSCs supernatant group. The diabetic rat model was constructed by injecting with alloxan by tail vein and feeding with high-fat diet. Diabetic skin ulcer (DSU) rat model was constructed by scratching a wound and infusing suspension of Staphylococcus aureus. In the control group, the diabetic rats (n=15) were scratched to form a wound and treated by tail vein injection of 100μl PBS. In the PBS group, DSU rats (n=15) were treated by tail vein injection of 100μl PBS, and then 100μl PBS was dropped at the ulcer site. In the UCMSCs supernatant group, freeze-dried powder of UCMSCs supernatant was dissolved in 200μl PBS, 100μl of which was injected into the tail vein of DSU rats (n=15), and other 100μl was dropped at the ulcer site. After 5 days of the treatments, the levels of serum TNF-αwere detected by radioimmunoassay method, and the expression of TNF-αand caspase-3 in the ulcer tissues of rats was detected by polymerase chain reaction and Western Blot. Results The levels of TNF-αin the PBS group [(35.9±3.7)μg/L] were significantly higher than that of the control group [(11.4±4.9)μg/L] and the UCMSCs group [(14.7±6.6)μg/L] (all P<0.05). The levels of mRNA and protein expression of TNF-αand caspase-3 in the UCMSCs group were significantly lower than those of the PBS group (all P<0.05), and have no significant differences with respect to those of the control group (all P>0.05). Conclusions UCMSCs supernatant treatments can effectively down-regulate the expression of TNF-αand caspase-3 in ulcer tissue of DSU rats, and play an anti-inflammatory and anti-apoptotic effect.

Yanlong ZHANG : 0000-0001-8358-7166 Ping WANG : 0000-0001-7517-8688 Huajiang DONG : 0000-0002-4141-3077 Keqiang WANG : 0000-0003-4813-4537 Dingwei PENG : 0000-0001-8112-5902 Xiping YANG : 0000-0003-3583-813X Lixia ZHANG : 0000-0001-5745-7157 Tao JIANG : 0000-0002-6355-302X Yuetong HUI : 0000-0001-7248-5481 Lei WANG : 0000-0002-2931-8317 Chongzhi SHANG : 0000-0001-7207-9115 Huipeng MENG : 0000-0002-7947-1360 Dingwei PENG1*, Xiping YANG1*, Lixia ZHANG2*, Xingyi SONG3*, Tao JIANG3, Yuetong HUI3, Lei WANG4, Chongzhi SHANG1, Huipeng MENG5, Yanlong ZHANG5, Ping WANG5, Huajiang DONG3, Keqiang WANG5,6

Objective To investigate the protective effect of umbilical cord mesenchymal stem cells (UCMSCs) on traumatic brain injury (TBI) in rats.Methods Thirty healthy Sprague-Dawley rats (10 rats for each group) were randomly divided into normal control group (normal),model group (injection of saline after TBI) and UCMSCs transplantation group (injection of UCMSCs after TBI).The rats in experimental groups were sacrificed on the 10th day after UCMSCs transplantation.The percentage of UCMSCs in brain tissue was detected by flow cytometry.The pathological changes of brain tissue were observed by hematoxylin-eosin (HE) staining method.The expressions of vascular endothelial growth factor (VEGF),glial fibrillary acidic protein (GFAP) and brain-derived neurotrophic factor (BDNF) in brain tissue were measured by immunohistochemistry and immunofluorescence double staining.The neurological deficit was evaluated by neurological deficit degree.Results The percentage of CD90,CD73 and CD105 cells in the UCMSCs transplantation group was significandtly higher than that in the model group (0.4％ vs 0.1％,P＜0.05).The results of HE staining showed that the brain injury of the transplanted group was alleviated compared with the model group (P＜0.05).The VEGF of the brain tissue in injury area in the UCMSCs transplantation group was higher than that in the model group (P＜0.05).The number of GFAP and BDNF positive cells in the UCMSCs transplantation group was higher than that in the model group (P＜0.05),and the neurological deficit score was also higher than that in the model group (P＜0.05).Conclusions UCMSCs transplantation for the treatment of TBI rats can effectively reduce the vascular damage in the injury area and promote nerve recovery.

Objective To investigate the expression of S100B in cerebrospinal fluid of patients with acute non-specific myelitis(ANM)and the significance of S100B in assessing the condition and the status of the patient with ANM.Methods Eight patients with ANM admitted to the Affiliated Hospital of Logistics University of Chinese People′s Armed Police Forces from Aug.2015 to Aug.2016 were enrolled in this study.The expression of S100B in cerebrospinal fluid was meas-ured by proteomics technique and verified by enzyme-linked immunosorbent assay(ELISA).S100B in healthy volunteers (N)and acute non-specific myelitis(S1)and after treatment(S2)were analyzed by ELISA to verify the accuracy of the results.Results For patients with ANM in acute phase,multiple high density shadows could be found by MRI examination;after treatment the symptoms alleviated,and the diffuse high density lesions disappeared.Proteomics S100B:S1:S2=0.33;S1:N=9.60;radioimmunoassay S100B:compared with healthy volunteers,the expression of S100B in cerebrospinal fluid of ANM patients in the acute phase was significantly increased[(0.111 ± 0.009)μg vs(1.761 ± 0.121)μg](P<0.05). Compared with onset stage of patients with ANM,the content of S100B in cerebrospinal fluid was significantly decreased after treatment[(1.761 ± 0.121)μg vs(0.292 ± 0.011)μg](P<0.05).Conclusion S100B can be used as an impor-tant marker for assessing the condition of patients with ANM,which is significantly increased at the onset stage and returns after treatment.

目的:探讨电子射野影像装置(electronic portal imaging device,EPID)用于容积旋转调强放疗(volumetricmodulated arc therapy,VMAT)绝对剂量验证的可行性与在临床应用中的意义.方法:随机选取100例患者的VMAT治疗计划,用Eclipse软件导出EPID计划和水模体验证计划等中心平面的通量图.在2幅通量图中对应位置处,各随机选取30个待测量绝对剂量点(靶区内6个,靶区外扩5、10、20、30、50 mm的区域内和有效探测范围内各4个点),按照选取位置的不同将这些剂量点分成7组,每个点均测量EPID和指形电离室实测值,用单因素F检验比较2种方法7组数据的计划值与实测值准确率的差异.结果:7组剂量点的绝对剂量验证结果准确率基本均在98％以上,2种方法测量的7组数据的准确率均无统计学差异(F=0.032-3.189,P＞0.05).结论:EHD具有在线实时剂量验证的潜力和明显优势,能够大大提高多点绝对剂量验证的效率,可以用于VMAT治疗绝对剂量的验证.