目的 研究蓝萼香茶菜叶的化学成分,为进一步研究其生物活性提供依据.方法 利用硅胶柱层析对蓝萼香茶菜叶进行分离和纯化其化合物,用波谱分析方法鉴定其结构,并运用四甲基偶氮唑盐比色法对从该植物中分离得到的部分化合物进行了细胞毒活性筛选.结果 从蓝萼香茶菜叶中分离鉴定了10个化合物,分别是蓝萼香茶菜庚素(1)、蓝萼香茶菜辛素(2)、蓝萼香茶菜壬素(3)、蓝萼香茶菜甲素(4)、蓝萼香茶菜乙素(5)、毛叶醇(6)、entkaurane-3β,16β-diol(7)、木犀草素-7-甲醚(8)、胡麻素(9)、水杨酸(10).其中化合物1、4、5具有较强的细胞毒活性.结论 化合物1、2、3、6、7、9、10首次从蓝萼香茶菜中分离得到,且化合物1、4、5对肿瘤细胞的增殖具有明显的抑制作用.

A novel eremophilane-type sesquiterpenoid, named isoligularonic acid (1), was isolated from the roots and rhizomes of Ligularia intermedia Nakai. Its structure was elucidated by extensive spectroscopic analysis including 1D, 2D NMR, and HR-MS spectra.

Three new ent-kaurane diterpenoids, glaucocalyxin H (1), glaucocalyxin I (2), and glaucocalyxin J (3), together with four known diterpenoids (4-7), were isolated from the leaves of Isodon japonica Hara var. glaucocalyx. Their structures were elucidated by spectroscopic analysis, and the structures of compounds 2 and 3 were further confirmed by X-ray crystallographic analysis. Compounds 1, 4, and 5 were evaluated for their cytotoxicity in vitro against CE-1, U87, A-549, MCF-7, Hela, K-562, and HepG-2 human tumor cell lines. Compound 1 showed potent inhibitory activities against six tumor cell lines with IC50 values ranging from 1.86-10.95 mu M, and compounds 4 and 5 exhibited significant selective cytotoxicity on seven tumor cell lines.

Objective To study the chemical composition of Schisandra chinensis and provide guidance for further investigation of its bioactivity.Methods Compounds were separated and purified by silica gel column chromatography and their structures were determined by spectroscopic methods.Results Five compounds were isolated and identified from Schisandra chinensis.Three of them were sesquiterpenoids 6β,8β-dihydroxyeremophil-7(11)-en-12,8α-olide(1),6β,8α-dihydroxyeremophil-7(11)-en-12,8β-olide(2),Bullatantriol(3);one was isoflavonoid Daidzein(4),and the other one was trans-Cinnamic acid(5).Conclusion Compounds 1 and 2 were separated from Schisandra chinensis for the first time.

Objective To study the chemical constituents and bioactivity of Pterocypsela elata.Methods Pterocypsela elata extracts were separated and purified by silica gel column chromatography and their structures were determined by spectroscopic methods.Results Four sesquiterpenoids compounds were obtained and identified from Pterocypsela elata.They were 11β,13-dihydrolactucin acetate(1),9α-hydroxyleucodin-9-O-β-xylopyranoside(2),11β,13-dihydrolactucin(3),and Lactuside B(4).Conclusion Compounds 2 and 3 were isolated from Pterocypsela elata for the first time.

A new guaianolide named 8-α-acetoxy-15β- O-β-D-glucopyranosyl-guaia-1(10),3(4)- diene-2-one-6,12-olide and 12 known compounds were isolated from the roots of Pterocypsela elata (Hemsl.) C. Shih, and eight of which were isolated from this genus for the first time. The structure of the new compound was elucidated mainly by spectroscopic methods, including 2D NMR techniques.

Objective To study the chemical constituents of Ligularia intermedia from Henan Province.Methods Compounds were isolated and purified by silica gel chromatography and recrystallization,and their structures were identified by spectral analysis.Results Twelve compounds from L.intermedia were identified as 6β-hydroxy-eremophil-7(11)-en-12,8α-olide(1),6β,8βdihydroxy-eremophil-7(11)-en-12,8α-olide(2),6β,8α-dihydroxy-eremophil-7(11)-en-12,8β-olide(3),eremoligularin(4),8βhydroxyeremophil-7(11)-en-12,8α-olide(5),10β-hydroxy-eremophil-7(11)-en-12,8α-olide(6),eremophila-1(10),7(11),8(9)-triene12,8-lactone(7),caffeic acid(8),palmitic acid(9),oleanolic acid(10),β-sitosterol(11),and β-daucosterol(12).Conclusion Compounds 1—5 and 7—9 are obtained from this plant for the first time.

Corrected by: Isolation, Structural Elucidation, and Cytotoxicity of Three New ent-Kaurane Diterpenoids from Isodon japonica var. glaucocalyxPlanta Med 2013; 79(16): 1588-1588DOI: 10.1055/s-0033-1350998

A new ent-kaurane diterpenoid, 3α, 14β, 16α-trihydroxy-ent-kaurane (1), together with seven known diterpenoids (2–8), was isolated from the leaves of Isodon japonica. Their structures were elucidated by spectroscopic methods, including 1D and 2D NMR experiments, IR, HR-ESI-MS and X-ray crystallographic analysis.

In the title compound, C46H80O3, a natural ursane-type triperpenoid, four of the five six-membered rings adopt chair conformations; the fifth, which has a C=C double bond, adopts an approximate half-boat conformation. In the crystal, molecules are linked by O—H⋯O hydrogen bonds, forming chains along [010].

In the title compound, C46H80O3, a natural ursane-type triperpenoid, four of the five six-membered rings adopt chair conformations; the fifth, which has a C=C double bond, adopts an approximate half-boat conformation. In the crystal, molecules are linked by O—H...O hydrogen bonds, forming chains along [010].

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目的研究小叶忍冬Lonicera microphylla藤的化学成分。方法应用硅胶柱色谱及反复重结晶分离小叶忍冬藤的化学成分,通过现代波谱技术鉴定化合物的化学结构。结果从小叶忍冬藤中分离鉴定了11个化合物,其中8个黄酮类化合物,3个甾体类化合物,分别鉴定为木犀草素(1)、香叶木素(2)、圣草酚(3)、金圣草黄素(4)、芹菜素(5)、罗汉松双黄酮A(6)、柏木双黄酮(7)、香叶木素-7-O-β-D-葡萄糖苷(8)、β-谷甾醇(9)、(24S)-3β,5α,6β-豆甾烷-三醇(10)、(22E,24R)麦角甾-7,22-二烯-3β,5α,6β-三醇(11)。结论所有化合物均为首次从小叶忍冬中分离得到,其中化合物6、10、11为首次从忍冬属中分离得到。