BACKGROUND:Oral squamous cell carcinoma (OSCC) remains a rampant oral cavity neoplasm with high degree of aggressiveness. Aldo-keto reductase 1B10 (AKR1B10) that is an oxidoreductase dependent on nicotinamide adenine dinucleotide phosphate (NADPH) has been introduced to possess prognostic potential in OSCC. The present work was focused on specifying the involvement of AKR1B10 in the process of OSCC and its latent functional mechanism.METHODS:AKR1B10 expression in OSCC tissues and cells were detected by RT-qPCR and Western blot analysis. CCK-8 method, EdU staining, wound healing and transwell assays respectively assayed cell viability, proliferation, migration and invasion. Immunofluorescence staining and Western blot evaluated epithelial mesenchymal transition (EMT). Adenosine triphosphate (ATP) contents, glucose consumption and extracellular acidification rate (ECAR) were measured by relevant commercially available kits and Seahorse XF96 Glycolysis Analyzer, severally. The expressions of proteins associated with metastasis and glycolysis were examined with Western blot. Co-IP assay confirmed the binding between AKR1B10 and hexokinase 2 (HK2).RESULTS:It was observed that AKR1B10 expression was increased in OSCC tissues and cells. After AKR1B10 was knocked down, the proliferation, migration, invasion and EMT of OSCC cells were all hampered. Additionally, AKR1B10 silencing suppressed glycolysis and bound to HK2 in OSCC cells. Up-regulation of HK2 partially abolished the hampered glycolysis, proliferation, migration, invasion and EMT of AKR1B10-silenced OSCC cells.CONCLUSION:To sum up, AKR1B10 could bind to HK2 to accelerate glycolysis, thereby facilitating the proliferation, migration, invasion and EMT of OSCC cells.

Oral squamous cell carcinoma (OSCC) has gradually become a global public health issue in recent years. Therefore, the current study aimed to explore the mechanism of OSCC development and to identify a potential target that may be used in its treatment. The expression of protein kinase, membrane-associated tyrosine/threonine 1 (PKMYT1) and cyclin A2 (CCNA2) in SCC-9 cells was determined prior to and following transfection with short hairpin RNA targeting PKMYT1. Cell proliferation, colony-forming ability, migration and invasion were determined using Cell Counting Kit-8, colony formation, wound healing and Transwell assays, respectively. Furthermore, the expression of epithelial-mesenchymal transition (EMT)- and migration-related proteins were evaluated using western blot analysis. Additionally, co-immunoprecipitation was used to verify the binding of PKMYT1 and CCNA2. The results revealed that PKMYT1 was highly expressed in OSCC cells and that PKMYT1 knockdown could inhibit proliferation, colony formation, migration, invasion, EMT and CCNA2 expression in SCC-9 cells. In addition, PKMYT1 was demonstrated to bind to CCNA2, and knocking down PKMYT1 resulted in inhibitory effects on cell proliferation, colony formation ability, migration, invasion and EMT by downregulating CCNA2 expression. PKMYT1 was observed to regulate the proliferation, migration and EMT of OSCC cells by targeting CCNA2, which may be used in the future to improve OSCC treatment.

目的:利用傅里叶红外变换光谱对干燥综合症伴龋齿患者口腔内特征微生物进行快速鉴别.方法:选取白色念珠菌、发酵乳杆菌、变异链球菌、干燥奈瑟菌和短双歧杆菌作为标准菌株与20例干燥综合症同时伴有龋病的患者口中分离的疑似菌株进行比对,采用傅里叶红外变换光谱法与标准菌株进行红外光谱的采集,光谱经过基线校正、光谱平滑和归一化处理,并采用一阶导数聚类分析.结果:分离的5种疑似菌与5种标准菌在特征谱区能准确的各自聚为一类.结论:利用傅里叶红外变换光谱能够快速、简便的分析干燥综合症患者口腔中的目标菌.

Primary sicca syndrome (pSS) is a systemic autoimmune disease. However, its exact etiology and pathogenesis remain elusive. Various infectious factors have been identified to be closely associated with the occurrence and development of PSS. The present study aimed to assess the composition of the oral microbial flora of patients with pSS in China in order to provide guidance for treatment. The microbial flora of nine patients with pSS and five healthy controls from East China was evaluated in saliva samples using high-throughput sequencing. A high microbial diversity was detected in the pSS and control groups, with bacteroidetes, firmicutes and proteobacteria constituting the largest phyla in the two groups. Compared with the control group, bacteroidetes and actinobacteria were significantly more abundant in the pSS group, whereas proteobacteria were significantly less abundant. However, no significant differences in bacterial richness and diversity were observed between the two groups. According to a Kyoto Encyclopedia of Genes and Genomes linear discriminant analysis, genes regulating cell apoptosis and the immune and digestive systems were significantly upregulated in the pSS group compared with those in the control group. In conclusion, the present study provided basic data on the flora of the oral cavity in patients with pSS from East China and may serve as a reference for the treatment of this condition.

Objective..To identify oral microorganisms extracted from healthy young people by Fourier transform infrared spectroscopy (FT-IR).Methods:FT-IR fingerprint data of 50 typical strains,which were extracted from oral cavity of 30 healthy people,were analyzed by a series of methods like averaging,the first derivative,and vector normalization.They were analyzed by the cluster methods and compared with the standard strains.Results:Isolated strains were easily to be compared with the standard strains in the characteristic spectrum area at 3000-2800,1500-1400,and 1200-900cm 1.Conclusion:As a technique which was accurate,fast,convenient,and time-effect,FT-IR was a reliable method for preliminary evaluation of oral microorganisms.It has guiding significance in establishing an early warning system for diseases caused by oral microorganisms.

The packaging technology of thick sauce-like food′s metal cans was deeply discussed,and a self-developed set of high-speed combined device comprising of filling and sealing was introduced.The design scheme,key structural and technical principle of this device was also detailed.The equipment consisted of filling and sealing system in the overall design,equipped with 36 filling valves and 8 sealed canned cover.The actual test showed that production capacity of this equipment was 600 cans / min,filling accuracy up to±1%,the can loss rate 0.11%,cover loss rate 0.15%,packaging product qualified rate 99.8%.

Objective To examine the expression of VEGF in oral lichen planus(OLP)and oral squamous cell carcinoma.Methods The expression of VEGF was observed in 10 cases of normal oral mucosa,25 cases of OLP,11 cases of OLP with dysplasia and 14 cases of oral squamous cell carcinoma(OSCC) by immunohistochemistry.Results The expression of VEGF in OLP was significantly lower than those in normal oral mucosa,OLP with dysplasia and OSCC(P<0.05).The expression of VEGF in OLP with dysplasia and OSCC was significantly higher than that in normal oral mucosa.Conclusion The abnormal expression of VEGF may play a role in the development and carcinogenesis of OLP.

According to the actual needs and cam design requirements of the production,it used check-point method to take a series of points,through the Matlab fitting of these points obtained trajectory curve equation,and then draw the trajectory curve of UG to arrive at an approximate trajectory trough,through a trial machine operation,good performance.

Obiective To observe the changes of gene expression profile of oral lichen planus after Yupingfeng oral liquid treatment by cDNA microarray.Methods The total RNAs were isolated from normal oral mucosa tissue,oral lichen planus tissue and oral lichen planus tissue after Yupingfeng oral liquid treatment.Then they were reversely transcribed to prepare the hybridization probe.The mixed probes were hybridized with the BiostarH-40s cDNA microarray.After washing,the cDNA microarray was scanned for the fluorescent signals by ScanArray 4000 scanning apparatus.Genepxix Pro 3.0 software was used to detect and select the genes differentially expressed in oral lichen planus after Yupingfeng oral liquid treatment.Results The result indicated that the expressions of 5 genes were up-regulated and expressions of 3 genes were down-regulated after Yupingfeng oral liquid treatment.Conclusions Yupingfeng oral liquid plays an impotant role in intervening the differentially expressed genes in OLP and the genes intervened are mainly transcription factors.

Because of the advantages of excellent barrier properties,mechanical properties,good surface decoration and convenient waste disposal,metal packaging containers have been widely used in food packaging,especially in the production of canned food,a large number of metal containers are used as packaging.However,the sealing of metal containers in metal containers is an important element,the use of three-dimensional simulation technology for the design,with relatively simple and clear.In this paper,canned fish tank sealing of oval bodies has been studied as an example,and achieved satisfactory results.

From the angle of media ecology, the deterioration of ecological environment leads to the uneven develop- ment of communication of current minority nationality culture. So we must make a systematic study and put forward some suggestions for this question.