ObjectiveTo analyze and compare the feasibility of using ELISA and IHA in our laboratory for testing specific TOX-Ab in laboratory rabbit.Methods Used ELISA and IHA methods for a parallelized testing of the specific TOX-Ab contained in both the positive serum of 12 rabbits infected with toxoplasma gondii and negative serum of 30 uninfected rabbits, thus to compare the sensibility, specificity, testing efficiency and Youden index between the two methods before applying Kappa values for a consistency evaluation.Results The sensibility and specificity of IHA was 41.67% and 93.33% respectively, while that for ELISA are both 100%. The sensibility, specificity, testing efficiency and Youden index for ELISA are higher than that of IHA. The total consistency rate for such two methods was 78.57% with Kappa value equals to 0.3998.Conclusion The consistency between the use of ELISA and IHA for this parallelized testing is relatively poor, and ELISA is notably better than IHA in terms of sensitivity and specificity, therefore it is more suitable to be applied in the testing of the specific TOX-Ab in laboratory animal-rabbit.

R2R3-MYB, bHLH, and WD40 proteins have been shown to control multiple enzymatic steps in the biosynthetic pathway responsible for the production of flavonoids, important secondary metabolites in Camellia sinensis. Few related transcription factor genes have been documented. The presence of R2R3-MYB, bHLH, and WD40 were statistically and bioinformatically analyzed on 127,094 C. sinensis transcriptome unigenes, resulting in identification of 73, 49, and 134 genes, respectively. C. sinensis phylogenetic trees were constructed for R2R3-MYB and bHLH proteins using previous Arabidopsis data and further divided into 27 subgroups (Sg) and 32 subfamilies. Motifs in some R2R3-MYB subgroups were redefined. Furthermore, Sg26 and Sg27 were expanded compared to Arabidopsis data, and bHLH proteins in C. sinensis were grouped into nine subfamilies. According to the functional annotation of Arabidopsis, flavonoid biosynthesis in C. sinensis was predicted to include R2R3-MYB genes in Sg4 (6), Sg5 (2), and Sg7 (1), as well as bHLH genes in subfamily 2 (2) and subfamily 24 (5). The wide evolutionary gap prevented phylogenetic analysis of WD40s; however, a single gene, CsWD40-1, was observed to share 80.4 % sequence homogeny with AtTTG1. Analysis of CsMYB4-1, CsMYB4-2, CsMYB4-3, CsMYB4-4, CsMYB5-1, and CsMYB5-2 revealed the interaction motif [DE]Lx2[RK]x3Lx6Lx3R, potentially contributing to the specificity of the bHLH partner in the stable MYB–bHLH complex. Full-length end-to-end polymerase chain reaction (PCR) and quantitative reverse transcriptase (qRT)-PCR were used to validate selected genes and generate relative expression ratio profiles in C. sinensis leaves by developmental stage and treatment conditions, including hormone and wound treatments. Potential target binding sites were predicted.

Gene therapy drugs have become a key of exploitation for biotechnology-derived pharmaceuticals,and adenovirus vectors are common used to mediate gene therapy in clinic.They could induce toxicity reaction accompanied with their therapeutic effect simultaneously.This review will focus on the toxicity reaction and activation of innate and acquired immune system induced by adenovirus vectors and the related drugs to elucidate their toxicity and the related mechanism.

Tea (Camellia sinensis) is a commercially important crop that contains valuable secondary metabolites. To understand the molecular regulation of secondary metabolism in tea, we selected and analyzed two cell lines of tea callus (Yunjing63Y and Yunjing63X) that showed different morphological characteristics and catechin contents. Yunjing63Y callus was yellow and tight, while yunjing63X callus was white and loose. HPLC analyses showed that Yunjing63Y contained 3.71 times higher levels of catechins than Yunjing63X. Using cDNA amplified fragment-length polymorphism (cDNA-AFLP) we identified 68 genes that were differentially expressed between the two lines. Of the 68 differentially expressed ESTs, 40 showed higher expressions in Yunjing63Y and 28 showed higher expressions in Yunjing63X. BLASTX comparisons classified these ESTs into seven functional groups; phenylpropanoid metabolism (2.9%), UDPG-dependent glucosyl transferase (8.8%), transcription factors (11.8%), transporters (13.2%), signal transduction (19.1%), other metabolism (26.5%), and unknown (17.7%). We used qRT-PCR to validate the expression of genes and ESTs, and found that genes associated with flavan-3-ols biosynthesis and metabolism were expressed at higher levels in Yunjing63Y than in Yunjing63X. In addition, the expression of ESTs associated with flavonoid biosynthesis, regulation and transport were higher in Yunjing63Y than in Yunjing63X. The full-length cDNA of a EST coding for a putative MYB transcription factor was amplified using rapid amplification of cDNA ends (RACE). The resulting 1270bp long cDNA, named CsMYB1, contained a 933-bp ORF encoding a 310-amino acid protein with a predicted molecular weight of 105.27kDa and a predicted isoelectric point of 4.85 and showed highest homology to plant MYBs likely involved in stress signaling.

Anthocyanin reductase is a key enzyme in the biosynthesis of EC and EGC in tea plant.The open reading frame of anthocyanin reductase gene(ANR),which encoding a 337 amino acids protein,was cloned from tea plant(Camellia sinensis(L.) O.Kuntze) by RT-PCR.The deduced protein molecular weight was 37 kD and its theoretical isoelectric point was 6.54.The gene was cloned into the expression vector pET32a(+) for expression in prokaryotic cells.The SDS-PAGE results showed that the anthocyanin reductase peoteins was expressed in Escherichia coli rosetta.The optimal inducing conditions including time,temperature,IPTG concentration,ampicillin concentration were studied.The deduced protein was purified and its activity was detected by HPLC.