mRNA疫苗是具有免疫性、安全性及灵活性的基因疫苗.相比传统疫苗,mRNA疫苗能够刺激免疫系统产生平衡、长效的保护；被以Toll样受体为主的受体识别,进入细胞,在胞液中表达,通过刺激免疫系统产生B、T细胞等多种机制,达到预防流行病及抗肿瘤等作用.部分mRNA疫苗本身具有的疫苗佐剂特性,通过产生多种细胞因子等不同方式刺激免疫系统,增强免疫机体反应能力,缩短免疫应答时间,加大抗体合成释放能力,在新型疫苗制备具有极大的应用价值.本文系统介绍了mRNA疫苗的组成、研究进展及其优势.

Gene therapy drugs have become a key of exploitation for biotechnology-derived pharmaceuticals,and adenovirus vectors are common used to mediate gene therapy in clinic.They could induce toxicity reaction accompanied with their therapeutic effect simultaneously.This review will focus on the toxicity reaction and activation of innate and acquired immune system induced by adenovirus vectors and the related drugs to elucidate their toxicity and the related mechanism.

应激可以引起实验动物出现氧化应激.应激后机体多个环节能量代谢的改变是其引起氧化应激的机制之一.糖皮质激素参与了应激后氧化应激的发生.检测机体活性氧(ROS)水平,抗氧化能力及氧化应激损害标志物含量,可用来评估氧化应激发生及其危害.本文将就近年来实验动物应激如何引起氧化应激、其发生机制及监测方法等领域的研究进展作一综述.

Objective To investigate the diurnal variations of hematological and biochemical parameters in cynomolgus monkeys.Methods The blood samples were collected in 12 cynomolgus monkeys(6 of each sex) at 9:30(am) ～ 10:30(am),12:30(pm) ～ 13:30(pm),15:30(pm) ～ 16:30(pm) and 19:30(pm) ～ 20:30(pm).The hematological and biochemical parameters were determined with automated hematological and biochemical analyzers respectively.The plasma cortisol levels were determined by high-performance liquid chromatography(HPLC).Results White blood count(WBC) and blood urea nitrogen(BUN) increased significantly in the afternoon in male and female monkeys.Hemoglobin(HGB) and hematocrit(HCT) markedly reduced in the afternoon in female monkeys.The activities of asparate aminotransferase(AST) and alanine aminotransferase(ALT) significantly increase in the afternnon in female monkeys.The serum glucose and plasma cortisol reduced significantly in the afternoon in male monkeys.Conclusions Parts of the hematological and biochemical parameters show obvious diurnal variation in male and female cynomolgus monkeys.

Objective To evaluate the influence of long distance transportation on plasma cortisol,serum biochemical parameters,malondiadehyde(MDA)and total antioxidant capacity(TAOC)in cynomolgus monkeys(Macaca fascicularis).Methods Blood samples were collected from male cynomolgus monkeys at different time points of pre-and post-transportation and were used to determine serum biochemical parameters,MDA and TAOC.Results ALT,AST and LDH increased significantly after transportation(P0.05).ALP,GLU and TBIL increased but there was no significant difference.BUN,CREA,TP and ALB decreased significantly after transportation(P0.05).TCHO and CK decreased but there was no significant difference.Except for CK and LDH,all serum biochemical parameters returned to normal levels on the twenty-ninth day.Serum MDA increased gradually after transportation,reached a peak value on the second day(P0.01)and became stable on the twenty-ninth day.Serum TAOC decreased gradually after transportation,reached the trough value on the second day(P0.05)and became stable on the twenty-ninth day.Conclusions Transportation stress could affect the values of most of the serum biochemical parameters and cause oxidative changes in cynomolgus monkeys after transportation.At least,an adaptation period of 29 days should be guarantied for the serum biochemical parameters to return to their normal values and the oxidative versus anti-oxidative status get back to their balance.