Objective To evaluate the value of combined detection of negative costimulatory molecule B7-H4 and carcinoembryonic antigen (CEA) in diagnosing malignant and benign pleural effusion.Methods Ninety-seven pleural effusion specimen were collected, 55 of which were diagnosed as malignant pleural effusion and 42 were benign pleural effusion.Enzyme-linked immunosorbent assay(ELISA) was used to examine the concentration of B7-H4 and CEA in pleural effusion.Electro-chemiluminescence immunoassay was used to detect the CEA level in pleural effusion.Receiver operating characteristic (ROC) curve was established to analyze and evaluate the single or combined detection of B7-H4 and CEA in diagnosing malignant and benign pleural effusion.Results The concentrations of B7-H4 and CEA in malignant pleural effusion (MPE) group were (60.08±35.04) ng/ml and (41.49±37.16) ng/ml, respectively, obviously higher than (27.26±9.55) ng/ml and (2.41±0.94) ng/ml of benign pleural effusion (BPE) group (both P<0.01).Area under curve (AUC) of B7-H4 was 0.884 in MPE groupand the diagnostic sensitivity and specificity were 81.8% and 90.5%, respectively, at the optimized cut off value of 37.25 ng/ml.Likewise, area under curve (AUC) of CEA was 0.954 and the sensitivity and specificity were 87.3% and 95.2%, respectively, at the cut off value of 4.18 ng/ml.When B7-H4 >37.25 ng/ml or CEA>4.18 ng/ml, the sensitivity of diagnosis as MPE was down-regulated to 90.9% and the specificity was elevated to 88.1%.When B7-H4 >37.25 ng/ml and CEA>4.18 ng/ml, the sensitivity of diagnosis as MPE was down-regulated to 78.2% and the specificity was elevated to 97.6%.The sensitivity and specificity of combined detection of B7-H4 and CEA to diagnose MPE were elevated to 90.9% and 97.6%, respectively.The level of B7-H4 in MPE and BPE were both positively correlated with CEA (r=0.670, P=0.001 in MPE and r=0.002, P=0.001 in BEP).Conclusions B7-H4 is a potential tumor marker in diagnosing the benign and malignant pleural effusion.Although the diagnostic value of B7-H4 may not precede to CEA, the combined detection of B7-H4 and CEA can improve the diagnostic sensitivity and specificity of MPE.

目的 探讨血清神经元特异性烯醇化酶(neuron specific enolase,NSE)、糖类抗原(carbohydrate antigen,CA) 125、癌胚抗原(carcinoembryonic antigen,CEA)、非小细胞肺癌抗原(cytokeratin 19 fragment,CYFRA21-1)联合检测对小细胞肺癌(small cell lung cancer,SCLC)的诊断价值.方法 SCLC患者51例(SCLC组)和同期体检健康者25例(对照组),采用电化学发光法检测2组血清NSE、CA125、CEA、CYFRA21-1、CA19-9、CA72-4水平,应用ROC曲线进行分析和评价.结果 SCLC组NSE[(60.22±19.62) μg/L]、CA125[(49.79±9.78) u/mL]、CEA[(6.46±1.02)μg/L]、CYFRA21-1[(3.95±0.85)μg/L]高于对照组[(13.47±4.41)μg/L、(11.49±3.32)u/mL、(1.93±0.75)μg/L、(2.14±0.64)μg/L],差异有统计学意义(P＜0.01),SCLC组CA19-9[(15.28±4.39) u/mL] 、CA72-4[(2.89±0.36) u/mL]与对照组[(8.42±1.03)u/mL、(1.82±0.41) u/mL]比较差异无统计学意义(P＞0.05);血清NSE、CA125、CEA、CYFRA21-1在SCLC组的AUC分别为0.905、0.853、0.778、0.705;ROC曲线分析显示NSE、CA125、CEA、CYFRA21-1的临床诊断临界点分别为23.23 μg/L、19.71 u/mL、2.64 μg/L、3.24 μg/L,单项检测时,NSE、CA125、CEA、CYFRA21-1的灵敏性分别为74.51％ 、64.71％ 、64.71％ 、50.98％,特异性分别为100.00％、96.00％、88.00％、92.00％,4项指标联合检测时灵敏性为94.12％,特异性为84.00％.结论 血清NSE是诊断SCLC的一个较理想指标;检测血清NSE、CA 125、CEA、CYFRA21-1水平对诊断SCLC有重要意义,4项联合检测灵敏度较单项检测升高,但特异性下降.

Objective To explore the relationship between neutrophil -to -lymphocyte ratio and clinicopathological charac-teristics of patients with prostatic cancer and the prognostic value.Methods The clinicopathological data of 60 patients with prostate cancer treated in the First Affiliated Hospital of Zhengzhou University from January of 201 2 to December of 201 3 were retrospectively analyzed.The patients were divided into high NLR (NLR≥3,n =30)group and low NLR(NLR <3,n =30) group.The median survival time between the two groups were compared.Results The differences of TNM staging,degree of differentiation and Gleason scores between the two groups were statistically significant(P <0.05).The median survival time of high NLR group and low NLR group were 1 8 and 25 months respectively,the difference was statistical significant (P <0.05). Conclusion NLR was related to Gleason scores,TNMstaging and degree of differentiation of patients with prostatic cancer.It may be an effective index to evaluate the prognosis of patients with prostate cancer.

BACKGROUND:Studies have shown that cancer stem cels play an important role in tumor invasion and metastasis, but studies on the role of gastric cancer stem cels in invasion and metastasis of gastric cancer cels were rarely reported. <br> OBJECTIVE:To study the effect of gastric cancer stem cels CSC-G on invasion and metastasis of gastric cancer cels. <br> METHODS: Gastric cancer stem cels CSC-G and gastric cancer cels SGC7901 were culturedin vitro for 10 days folowed by spherical colony formation assay, western blot assay for detecting OCT4, SOX2, E-cadherin and CD44 protein expression levels in gastric cancer stem cels CSC-G and gastric cancer cels SGC7901, and Transwel assay for detecting the invasion and migration of gastric cancer stem cels and gastric cancer cels SGC7901. <br> RESULTS AND CONCLUSION:Gastric cancer cels SGC7901 in RPMI1640 medium presented with adherent growth and were quadrilateral or polygonal after passage; gastric cancer stem cels CSC-G in serum-free medium presented with suspension growth, and adherent gastric cancer stem cels were spindle-shaped or round. Compared with gastric cancer cels SGC7901, the protein expressions of OCT4, SOX2 and CD44, the number of cancer cel spheres and the number of trans-membrane cels were significantly increased in the gastric cancer stem cels CSC-G (P < 0.05), and the expression of E-cadherin protein was significantly decreased (P < 0.05). These findings indicate that the gastric cancer stem cels CSC-G can be successfuly cultured in vitro, and have enhanced invasion and migration compared with the gastric cancer cels SGC7901, which play an important role in gastric cancer invasion and metastasis.

目的：探讨星形胶质细胞上调基因-1（AEG-1）和淋巴增强因子-1（LEF-1）在人结肠癌组织中的表达，并分析其与临床病理特征的关系。方法：采用免疫组化法检测78例人结肠癌组织及78例相应癌旁正常组织中AEG-1和LEF-1的表达，并分析其临床病理意义。结果： AEG-1和LEF-1在结肠癌组织中的阳性表达率分别为73．1％（57／78）和67．9％（53／78），均明显高于癌旁正常组织（P ＜0．001）。 AEG-1的表达与患者的组织分化程度、淋巴结转移、远处转移及 TNM 分期有关（P ＜0．05），LEF-1的表达与患者的淋巴结转移、远处转移及 TNM 分期有关（P ＜0．05）。结肠癌组织中 AEG-1与 LEF-1的表达呈正相关（r ＝0．326，P ＝0．004）。结论： AEG-1和LEF-1的异常表达与结肠癌的发生、发展、浸润及转移密切相关，联合检测更有利于结肠癌的早期诊断及预后的判断。

Objective To evaluate the effect and toxicity of S-1 in combination with Shenyi capsule for patients with advanced non-small cell lung cancer(NSCLC),received two or more prior chemotherapy regimens.Methods Fifty-five advanced NSCLC patients received second-line or further-line chemotherapy previously were enrolled in this study.Thirty-six cases were given S-1 orally(group E),the dosage was taken according to body surface,twice a day,after breakfast and dinner respectively,for 14 consecutive days,followed by a 7-day rest period,twenty-one days was a cycle,Shenyi capsule was administered orally at the same time.The effect and toxicity were evaluated after 2-cycle treatment.Another nineteen cases received Shenyi capsule only(group C).Results In group E,there was no complete response(CR),4 patients exhibited a partial response (PR),19 patients with stable disease (SD) and 13 with progressive disease(PD).The disease control rate(DCR =CR + PR + SD) was 63.9％.The main adverse reactions were bone marrow suppression and gastrointestinal reaction.The median progression free survival of group E and group C were 2.8 months and 2.0 months,the median overall survival were 6.0 months and 4.3 months respectively.Between the two groups,the PFS and OS was not statistically different.Conclusions For advanced NSCLC patients received second-line or further-line chemotherapy previously,S-1 in combination with Shenyi capsule has certain effect,its adverse reaction can be tolerated and it can be a good choice of the subsequent treatment after radiation and chemotherapy.

OBJECTIVE: To study expression of Serine/Threonine Kinase (AKT-2) and Pepsinogen C(PGC) in gastric carcinoma, and analyze its clinical significance. METHODS: Immunohistochemistry was used to examine expression of AKT-2 and PGC in 75 gastric carcinoma, 20 atrophic gastritis tissues and 15 para-carcinoma normal mucosa tissues. The correlation between AKT-2, PGC and the clinical pathologic factors was analyzed by chi-square test. RESULTS: The positive rates of AKT-2 in gastric carcinoma, atrophic gastritis tissues and para-carcinoma normal mucosa tissues gradually declined, were 70.7%(53/75) to 38.9%(7/20) and 26.7%(4/15). The expression of AKT-2 in gastric carcinoma tissues was significantly higher than that in atrophic gastritis tissues(P=0.003) and normal tissue (P=0.001), but there was no significant different between the latter two groups (P=0.875). Expression of AKT-2 was significantly correlated with differentiation status (P=0.019), infiltration depth (P=0.017), lymph node metastasis (P=0.027) and clinical stage of the cancer (P=0.030). The positive rates of PGC in gastric carcinoma, atrophic gastritis tissues and para-carcinoma normal mucosa tissues were 9.3%(7/75), 65.0%(13/20), 100.0%(15/15). The expression of PGC in gastric carcinoma tissues was significantly higher than that in atrophic gastritis tissues (P=0.000) and normal tissue (P=0.000), and there was significant different between the latter two groups (P=0.033). Expression of PGC was significantly correlated with differentiation status (P=0.037) and clinical stage (P=0.044) of the cancer (P<0.05). There was a negative correlation between AKT-2 and PGC (r=-0.297, P=0.01). CONCLUSIONS: The expression of AKT-2 in gastric carcinoma tissues is significantly higher than that in atrophic gastritis tissues and normal tissue, but the expression of PGC in gastric carcinoma tissues is significantly lower than that in atrophic gastritis tissues and normal tissue. The overexpression of AKT-2 and the lowexpression of PGC play important roles in the early diagnosis of gastric carcinoma.

Objective To study the expression and clinical significance of steroid receptor rcoaetivator-3 (SRC-3) and F-box/WD repeat-containing protein 7 (FBXW7) in cervical carcinoma.Methods Immunohistochemistry was used to examine the expressions of SRC-3 and FBXW7 in cervical carcinoma and normal cervical tissues.Results The positive rates of SRC-3 in cervical carcinoma and normal tissue were 33.3％ and 10.0％,there was no significant difference(P =0.083 ＞0.05),but with differentiation degree of cervical carcinoma gradually reduced,SRC-3 expression level had a increasing trend,and the expression of SRC-3 was significantly correlated with differentiation degree,clinical stage and lymph node metastasis(P ＜0.05).The positive rate of FBXW7 in cervical carcinoma and normal tissue was 48.3％ and 90.0％ respectively,there was significant difference(P ＜ O.05) ; and the expression of FBXW7 was significantly correlated with differentiation degree,clinical stage and lymph node metastasis (P ＜ O.05).And there was a negative correlation of SRC-3 with FBXW7 (r =-O.259,P =0.045).Conclusions SRC-3,FBXW7 may be a new indicators and therapeutic targets for early diagnosis and prognosis evaluation of cervical cancer.

Objective To compare the difference of patients' survival time,disease progressionfree survival,drug susceptibility,drug safety between pemetrexed and single symptomatic supportive treatment in patients with advanced malignant pleural mesothelioma.Methods Twenty patients with relapsing malignant pleural mesothelioma were randomly divided into two groups,11 cases were treated with pemetrexed 500 mg/m2 was administered to patients on day 1 cycle of a 3-weeks,15 cases were treated only with single symptomatic supportive treatment.Results There was no significantly difference in median survival time between treatment group and control group(8.land 7.6 months ; P =0.775).Median progression-free survival time was respectively 3.2 months and 1.7 months (P ＜ 0.005).Partial response rate was 18.2％ and 0％ and the disease control rate was 63.6％ and 13.3％.7％ patients had modest grade 3 and 4 hematologic toxicities,the chemotherapy was well tolerated.Conclusions The effect of pemetrexed on patients with advanced malignant pleural mosethelioma is good.

Objective: To understand the factors of risk and protection of liver cancer,and preliminarily evaluate the relationship between lifestyle,dietary factors and the occurrence of liver cancer.Methods: Using the method of 1∶ 1 paired case-control study design,73 cases liver cancer patients and 73 cases of not liver cancer patients were investigated by questionnaire,and the effective factors of liver cancer were analyzed by Chi-square test and Logistic regression.Results: Drinking and aflatoxin poison pollution were the main risk factors for the occurrence of liver cancer,and OR values were 5.8 and 3.25 respectively(P0.05);while eating more vegetables and drinking much of tea could are protective factors,and OR values were 0.34 and 0.39 respectively(P0.05).Conclusion: Drinking and aflatoxin poison pollution were risk factors for liver cancer,but eating more vegetables and drinking much of tea could are protective factors.

Aim:To investigate the expressions of F-box/WD repeat-containing protein 7(FBXW7) and Cyclin E in gastric carcinoma.Methods:Immunohistochemistry SP method was used to examine the expressions of FBXW7 and Cyclin E proteins in 75 cases of gastric carcinoma and 25 cases of para-carcinoma normal tissues.Results:The positive expression rates of FBXW7 and Cyclin E proteins in gastric carcinoma and para-carcinoma normal tissues had significant differences(χ2 =10.606 and 7.692,P=0.001 and0.006),and the expression of FBXW7 in carcinoma tissue was significantly related with the differentiation degree(χ2 = 7.440,P = 0.006),lymphatic metastasis(χ2 = 4.688,P = 0.030) and the clinical stage(χ2 = 4.601,P = 0.032).The expression of Cyclin E was significantly related with invasion depth(χ2 = 4.902,P = 0.027),lymphatic metastasis(χ2 = 4.601,P = 0.032) and clinical stage(χ2 = 4.408,P = 0.036).The expression of FBXW7 was negatively associated with that of Cyclin E(rP =-0.459,P < 0.001) in gastric carcinoma tissue.Conclusion:FBXW7 and Cyclin E may play important roles in the development of gastric carcinoma.

Aim:To evaluate the efficacy of FOLFOX6 regimen combined with Fufangbanmao capsules for advanced primary hepatocarcinoma treatment.Methods:Fifty five untreated patients with advanced primary hepatocarcinoma were collected,among whom,31 were treated with FOLFOX6 regimen,24 were treated with FOLFOX6 combined with Fufangbanmao capsules.Results:The total response rates(RR) of the FOLFOX6 group and combination group were 12.9% and 16.7%,and disease control rate(DCR) were 48.4% and 54.2%,respectively.No significant differences were found in RR and DCR between the two groups(χ20.001,χ2=0.181,P0.05).Media TTP was 4.9 months in FOLFOX6 group,shorter than that(7.4 months) in combination group(Z=2.220,P=0.026);improvement rate of KPS scores was 51.6% in FOLFOX6 group,lower than that(74.2%) in combination group(χ2=4.428,P=0.035).Granulocytopenia and anemia incidences were significantly lower in combination group compared with those of FOLFOX6 group(χ2=7.913,4.045,P0.05).Conclusion:The synergism of FOLFOX6 and Fufangbanmao capsules could improve quality of life,and reduce the incidence of granulocytopenia and anemia during the treatment of advanced primary hepatocarcinoma.

目的:研究F框/WD-40域蛋白7(FBXW7)和Cyclin E蛋白在胃癌组织中的表达.方法:应用免疫组化SP法检测75例胃癌和25例癌旁正常胃组织中FBXW7和Cyclin E蛋白的表达.结果:胃癌、癌旁正常胃组织中FBXW7 和Cyclin E蛋白阳性表达率差异有统计学意义(χ2=10.606和7.692,P=0.001和0.006).FBXW7蛋白的表达与胃癌的分化程度(χ2=7.440,P=0.006)、淋巴结转移(χ2=4.688,P=0.030)、TNM分期(χ2=4.601,P=0.032)有关;Cyclin E蛋白的表达与胃癌的浸润深度(χ2=4.902,P=0.027)、淋巴结转移(χ2=4.601,P=0.032)、TNM分期(χ2=4.408,P=0.036)有关.胃癌组织中FBXW7与Cyclin E蛋白的表达呈负关联(rP=-0.459,P<0.001).结论:FBXW7和Cyclin E可能在胃癌的发生发展中起重要作用.

Objective: To observe the clinical therapeutic effect and adverse reaction of 125I radioactive seed treatment to gastric cancer patients with liver metastasis.Methods: 48 cases of gastric cancer patients who had liver metastasis after operation were randomly divided into two groups.125I radioacte cases of the treatment group included 24 cases Another 24 cases of the control group were treated were embedded in liver metastasis cancer when the treatment stated for one time with oxaliplatin and Shiroda for at least 4 cycles.The curative effect in the near future and adverse reaction were analyzed.Results: Effective rate of the treatment group was 70.8%(17/24),and the control group was 37.5%(9/24).The statistical difference was significant(χ2=5.37,P=0.02),Endurable pain and low grade fever were found in the treatment group.No adverse reaction were found in the treatment group while adverse reaction such as gastrointestinal reaction,hand foot syndrome were found in the control group.Conclusion: Treatment of liver metastasis in gastric cancer patients with 125I radioactive particles had a significant curative effect in the near future and has no obvious adverse reaction.It is easily accepted by the patients and suitable for popularization.

Objective To study the expression of F-box/WD repeat-containing protein 7(FBXW7),fatty acid synthase(FAS) and minichromosome maintenance protein7(MCM7) in colorectal carcinoma and the related clinical significance.Methods Immunohistochemistry method was used to examine the expression of FBXW7,FAS and MCM7 in the colorectal carcinoma,colorectal adenoma and para-carcinoma normal mucosa tissues.Results The positive rates of FBXW7 in the colorectal carcinoma,adenoma and normal tissues were 58.2%,75.0%,and 88.9%,respectively,with significant difference found between the colorectal carcinoma and normal mucosa tissues(P 0.05);and FBXW7 expression was significantly correlated with the differentiation degree(χ2 =10.516,P = 0.001),lymphatic metastasis(χ2 = 4.489,P = 0.034) and the tumor size(χ2 = 9.974,P = 0.002).The positive rates of FAS in the colorectal carcinoma,adenoma and normal tissue were 94.3%,75.0%,and 55.6%,respectively,with significant difference found between the colorectal carcinoma and normal mucosa tissues(P 0.01);and FAS expression was significantly correlated with the patient age(χ2 = 7.643,P = 0.006).The positive rates of MCM7 in the colorectal carcinoma,adenoma and normal tissue were 95.8%,66.7%,and 22.2%,respectively,with the difference being significant between the three groups(P 0.01).FBXW7 expression was negatively correlated with that of FAS and MCM7(r =-0.276,P = 0.008;r =-0.443,P = 0.000),and FAS expression was positively correlated with MCM7 expression(r = 0.228,P = 0.024).Conclusion FBXW7,FAS and MCM7 might be new markers for early diagnosis and prognosis prediction of colorectal carcinoma;they may also serve as new therapeutic targets for colorectal carcinoma.

目的 探讨血清CA125、HE4检测联合影像学检查(彩超、CT、MRI)在卵巢上皮癌诊断中的临床价值.方法 收集2009年6月至2010年8月郑州大学第一附属医院肿瘤科、妇产科及门诊70例女性病例和30例妇性健康体检者及志愿者:①卵巢癌组42例均为卵巢上皮性癌,早期(Ⅰ期+Ⅱ期)19例、晚期(Ⅲ期+Ⅳ期)23例;②卵巢良性疾病组28例;③正常对照组30例.检测3组病例血清CA125、HE4含量,并行盆腔影像学检查.结果 ①卵巢癌组血清CA125和HE4水平[(290.91±212.42)U/ml、(300.94±127.95)pmol/L]明显高于正常对照组[(28.23±14.84)U/ml、(70.86±35.12)pmol/L]和卵巢良性疾病组[(30.70±17.43)U/ml、(78.52±33.52)pmol/L](P<0.01);晚期卵巢癌血清CA125和HE4水平[(379.61±244.29)U/ml、(355.85±139.57)pmol/L]高于早期患者[(183.55±87.28)U/ml、(234.47±70.46)pmol/L](P<0.01);早期卵巢癌血清CA125、HE4高于卵巢良性疾病组(P<0.01);卵巢癌术后1个月血清CA125和HE4[(62.04±23.03)U/ml、(99.71±36.86)pmol/L]较术前明显下降(P<0.01).②联检"CA125+HE4"、"CA125+HE4+影像检查"在卵巢癌中的阳性检出率分别为95.2%、97.6%,较任一单检的阳性检出率(CA125 76.2%、HE4 78.6%、影像检查88.1%)增高(P<0.05).③卵巢癌组中,联检"CA125+HE4"及"CA125+HE4+影像检查"的灵敏度分别为95.2%、97.6%;联检"CA125+HE4"准确率(93%)最高,血清HE4特异性(94.8%)最高.结论 卵巢癌患者血清CA125、HE4升高,CA125、HE4及影像学检查组合联检能进一步提高卵巢癌诊断的敏感性和准确性.

Aim:To detect the serum levels of blood-lipid,lipoprotein,carcinoembryonic antigen (CEA) and carbohydrate antigen(CA199) of gastric carcinoma patients.Methods:A total of 158 cases of gastric cancer,including 62 cases treated only by chemotherapy directly(38 metastasis cases and 24 no metastasis cases) and 96 cases treated operation and adjuvant chemotherapy(15 cases in pathologic stageⅠ,25 cases in stage Ⅱ,33 cases in stage Ⅲ ,23 cases in stage Ⅳ),were to detect the serum levels of TG,TC,HDL,LDL,CEA,and CA199.100 normal casesand 80 cases of benign disease(gastritis and gastric ulcer) were the controls.Results:The levels of TG,CEA,CA199 in gastric cancer group were higher than those in the other two groups (P0.05),while the levels of TC,HDL,LDLwere lower (P0.05).The levels of TC,LDL of gastric cancer group after operation were higher than those before operation (t=20.315,24.557,P0.001).Differences in the levels of TC,HDL,LDL,CEA,CA199 among different pathologic stages were significant in gastric cancer group(P0.05).The levels of the indexes mentioned above were not related with the therapy(P0.05).Conclusion:Blood-lipid and lipoprotein could be helpful for the diagnosis and adjudging the stages of clinical pathology of gastric cancer.

Objective To study expression of F-box/WD repeat-containing protein 7(FBXW7) and steroid receptor rcoaetivator-3(SRC-3) in gastric carcinoma and to examine its clinical significance.Methods Immunohistochemistry was used to examine expression of FBXW7 and SRC-3 in gastric carcinoma and para-carcinoma normal mucosa tissues.Results Positive rates of FBXW7 in gastric carcinoma and normal tissue were 46.7% and 90.0%,respectively,with the difference being significant(P0.01).Expression of FBXW7 was significantly correlated with differentiation status,lymph node metastasis and clinical stage(P0.05) of the cancer.Positive rates of SRC-3 in gastric carcinoma and normal tissue were 48.3% and 10.0%,respectively,showing a significant difference(P0.01).Expression of SRC-3 was significantly correlated with the depth of invasion,lymph node metastasis and clinical stage of the cancer(P0.05).There was a negative correlation between FBXW7 and SRC-3(r=-0.370,P=0.004).Conclusions FBXW7 and SRC-3 can be used as new markers for early diagnosis and prognosis prediction of gastric carcinoma.They may also serve as new therapeutic targets for gastric carcinoma.

Objective: To study the expression of F-box/WD repeat-containing protein 7(FBXW7), fatty acid synthase (FAS) and minichromosome maintenance protein7 (MCM7) in colorectal carcinoma and the related clinical significance. Methods: Immunohistochemistry method was used to examine the expression of FBXW7, FAS and MCM7 in the colorectal carcinoma, colorectal adenoma and para-carcinoma normal mucosa tissues. Results: The positive rates of FBXW7 in the colorectal carcinoma, adenoma and normal tissues were 58.2%, 75.0%, and 88.9%, respectively, with significant difference found between the colorectal carcinoma and normal mucosa tissues (P<0.05); and FBXW7 expression was significantly correlated with the differentiation degree(χ2=10.516, P=0.001), lymphatic metastasis (χ2=4.489, P=0.034) and the tumor size(χ2=9.974, P=0.002). The positive rates of FAS in the colorectal carcinoma, adenoma and normal tissue were 94.3%, 75.0%, and 55.6%, respectively, with significant difference found between the colorectal carcinoma and normal mucosa tissues (P<0.01); and FAS expression was significantly correlated with the patient age(χ2=7.643, P=0.006). The positive rates of MCM7 in the colorectal carcinoma, adenoma and normal tissue were 95.8%, 66.7%, and 22.2%, respectively, with the difference being significant between the three groups (P<0.01). FBXW7 expression was negatively correlated with that of FAS and MCM7(r= -0.276, P=0.008; r= -0.443, P=0.000), and FAS expression was positively correlated with MCM7 expression(r=0.228, P=0.024). Conclusion: FBXW7, FAS and MCM7 might be new markers for early diagnosis and prognosis prediction of colorectal carcinoma; they may also serve as new therapeutic targets for colorectal carcinoma.

目的研究mcm7、mcm5和cyclinE在直肠癌组织中的表达及临床意义。方法选取术前未经放化疗的直肠腺癌手术标本104例、直肠腺瘤28例及癌旁正常黏膜组织36例,采用免疫组化S-P法分别检测mcm7、mcm5、cyclin E的表达水平,并对104例直肠癌标本按照有无淋巴结转移等进行分组比较,分析其与直肠癌临床病理参数的关系。同时进行了三者的相关分析。结果直肠癌组织中mcm7、mcm5及cyclin E阳性表达率均明显高于对照组（P＜0.05）。28例直肠腺瘤中mcm7、mcm5及cyclin E阳性率分别为53.57%（15/28）、42.86%（12/28）、28.57%（8/28）,36例直肠癌旁正常组织中,mcm7、mcm5及cyclin E阳性率分别为27.78%（10/36）、22.22%（8/36）、11.11%（4/36）;而在104例直肠癌组织中,mcm7、mcm5及cyclin E阳性率分别为82.69%（86/104）、76.92%（80/104）、67.31%（70/104）。三者的表达与淋巴结转移、远处转移及癌组织分化程度、浸润深度有关（P＜0.05）,而与患者的性别、年龄及肿瘤的大小无明显关系。直肠癌组织中三者的表达成正相关（P＜0.05）。结论 mcm7、mcm5、cyclin E过表达可能在直肠腺癌的发生、发展中发挥重要作用。