Objective:To investigate the expression level of glucose 6-phosphate dehydrogenase (G6PD) in esophageal cancer cells and its effect on chemoresistance and molecular mechanism.Methods:Totally, 65 esophageal cancer specimens and corresponding adjacent tissues collected in our hospital from January 2021 to March 2022 were selected as the research objects. The expression levels of G6PD in esophageal cancer and adjacent tissues were analyzed by Western blotting. Paclitaxel resistant cell line Ec109/DOX was established by concentration gradient method in human esophageal cancer cell line EC109. EC109/DOX cell lines were infected with control short hairpin RNA (shRNA) and G6PD shRNA lentivirus respectively, and the control group and G6PD KD group were established. After treatment with G6PD inhibitor for 24 h, cell counting kit-8 (CCK-8) assay was used to analyze the cell proliferation ability of different treatments. The drug resistance of different treated cells was analyzed by flow cytometry. The expression of TPSP1 was analyzed by Western blotting. The measurement data between groups were compared by t-test. Results:The expression level of G6PD in adjacent tissues (0.97±0.16) was significantly lower than that in esophageal cancer tissues (2.34±0.28, t=32.670, P<0.05). The expression level of G6PD protein in paclitaxel resistant group (2.14±0.16) was significantly higher than that in paclitaxel sensitive group (2.56±0.22, t=8.280, P<0.05). The expression level of G6PD protein in EC109 cells (0.95±0.07) was significantly lower than that in EC109/DOX cells (2.21±0.23, t=11.870, P<0.05). The light absorption value of cells in the control group (1.25±0.03, 1.98±0.08) after 24 and 48 h of paclitaxel treatment was significantly higher than that in G6PD KD group (0.87±0.02, 1.42±0.07, t=21.631, 12.571, P<0.05]. After 48 h of paclitaxel treatment, the apoptosis ratio [(12.94±1.78)%] in the control group was significantly lower than that in the G6PD KD group [(34.56±4.03)%, t=10.961, P<0.05]. The apoptosis level of EC109/DOX cells treated with paclitaxel [(12.83±2.25)%] was significantly lower than that of EC109/DOX cells treated with 6-AN and paclitaxel [(43.36±4.53)%, t=13.500, P<0.05]. After 48 h of paclitaxel treatment, the expression level of glutathione S-transferase P1 (GSTP1) protein in the control group (12.94±1.78) was significantly lower than that in the G6PD KD group (34.56±4.03, t=6.490, P<0.05). The expression level of GSTP1 protein of EC109/DOX cells treated with paclitaxel (1.97±0.05) was significantly higher than that of EC109/DOX cells treated with 6-AN and paclitaxel (1.17±0.14, t=12.260, P<0.05). Conclusion:G6PD regulates the resistance of esophageal cancer cells to paclitaxel by regulating the expression of GSTP1 protein.

目的 探讨仑伐替尼联合经导管动脉栓塞化疗(TACE)治疗老年原发性肝癌患者的疗效.方法 将94例老年原发性肝癌患者按治疗方案不同分为对照组60例和观察组34例,对照组接受TACE治疗,观察组接受仑伐替尼联合TACE治疗.比较两组患者临床疗效、肝功能指标[天冬氨酸转氨酶(AST)、总胆红素(TBIL)、丙氨酸转氨酶(ALT)]、免疫功能指标(CD3+、CD4+及CD8+T细胞)、肿瘤标志物[血清甲胎蛋白(AFP)及癌胚抗原(CEA)]水平、不良反应及预后情况.结果 观察组患者客观缓解率、疾病控制率均高于对照组,差异均有统计学意义(P﹤0.05).治疗后,两组患者血清AST、TBIL、ALT水平均较本组治疗前升高,且观察组患者血清AST、TBIL、ALT水平均低于对照组,差异均有统计学意义(P﹤0.05).治疗后,两组患者CD3+、CD4+水平均较本组治疗前升高,CD8+水平均较本组治疗前降低,且观察组患者CD3+、CD4+水平均高于对照组,CD8+水平低于对照组,差异均有统计学意义(P﹤0.05).治疗后,两组患者血清AFP、CEA水平均较本组治疗前降低,且观察组患者血清AFP、CEA水平均低于对照组,差异均有统计学意义(P﹤0.05).两组患者不良反应总发生率比较,差异无统计学意义(P﹥0.05).观察组患者疾病进展时间(TTP)、总生存时间(OS)均优于对照组患者,差异均有统计学意义(P﹤0.05).结论 仑伐替尼联合TACE治疗老年原发性肝癌可获得显著效果,可显著降低AFP、CEA水平,抑制肿瘤进展,且联合治疗不增加严重不良反应,值得推荐.

目的:探讨不同组织学分级的原发性肝癌患者磁共振动态增强参数的差异.方法:回顾性分析郑州大学第一附属医院72例行手术切除的原发性肝癌患者的临床资料,72例中Ⅰ级7例,Ⅱ级19例,Ⅲ级30例,Ⅳ级16例.术前4周内均使用飞利浦Achieva 3.0 T磁共振成像仪行肝脏磁共振平扫和动态增强扫描,比较不同组织学分级的原发性肝癌患者的影像学特征.结果:不同组织学分级的肝癌患者临床基本资料的比较差异均无统计学意义(P>0.05).不同组织学分级的肝癌患者磁共振动态增强参数(达峰时间、信号增强幅度、峰值信号强度)比较,差异均有统计学意义(P<0.05);其中Ⅰ级达峰时间最长,信号增强幅度和峰值信号强度均最低,而Ⅳ级达峰时间最短,信号增强幅度和峰值信号强度均最高.结论:不同组织学分级的原发性肝癌患者磁共振动态增强参数存在一定差异,为研究肝癌患者的组织学特点提供了参考.

目的 参麦注射液已被证实对心肌细胞缺血再灌注(I/R)损伤具有保护作用,但其对肾I/R损伤的保护作用并不明确.文章旨在探讨参麦注射液对肾小管上皮细胞NRK-52E缺氧-复氧损伤的的影响和可能分子机制.方法 构建NRK-52E细胞缺氧复氧模型模拟肾I/R损伤过程.根据干预方法不同,将NRK-52E细胞分为正常对照组、I/R组(缺氧6 h-复氧24 h)、参麦+I/R组(参麦注射液处理30 min,缺氧6 h-复氧24 h)、miR-NC+I/R组(转染miR-NC,缺氧6 h-复氧24 h)、miR-106a+I/R组(转染miR-106a模拟物,缺氧6 h-复氧24 h)、anti-miR-NC+I/R组(转染anti-miR-NC,缺氧6 h-复氧24 h)、an-ti-miR-106a+I/R组(转染anti-miR-106a,缺氧6 h-复氧24 h)、参麦+miR-NC+I/R组(转染miR-NC,参麦注射液处理30 min,缺氧6 h-复氧24 h)、参麦+miR-106a+I/R组(转染miR-106a模拟物,参麦注射液处理30 min,缺氧6 h-复氧24 h).细胞计数试剂盒(CCK-8)检测细胞活力,流式细胞术检测细胞凋亡,实时荧光定量PCR(RT-qPCR)检测miR-106a的表达水平,Western blot法检测磷脂酰肌醇-3-羟激酶/蛋白激酶B(PI3K/AKT)蛋白表达.结果 与正常对照组Ki67蛋白(0.76±0.07)、细胞活性(0.83±0.05)比较,I/R组(0.33±0.02、0.35±0.02)显著降低(P<0.05),与正常对照组Cleaved-caspase-3蛋白表达(0.42±0.04)、凋亡率[(5.06±0.32)％]比较,I/R组[(0.86±0.07、(16.11±1.01)％]显著升高(P<0.05);与I/R组Ki67蛋白、细胞活性比较,参麦+I/R组(0.70±0.07、0.77±0.06)明显升高,与I/R组Cleaved-caspase-3蛋白表达、凋亡率比较,参麦+I/R组(0.54±0.04、7.24±0.53)明显降低(P<0.05).I/R组NRK-52E细胞miR-106a的表达水平(3.27±0.25)较正常对照组(1.00±0.08)和参麦+I/R组(1.53±0.08)显著升高(P<0.05).与miR-NC+I/R组比较,miR-106a+I/R组NRK-52E细胞miR-106a的表达水平显著升高,细胞活性、Ki67蛋白表达显著降低,细胞凋亡率、Cleaved-caspase-3蛋白表达显著升高(P<0.05);与anti-miR-NC+I/R组比较,anti-miR-106a+I/R组NRK-52E细胞miR-106a的表达水平显著降低,细胞活性、Ki67蛋白表达显著升高,细胞凋亡率、Cleaved-caspase-3蛋白表达显著降低(P<0.05).与参麦+miR-NC+I/R组比较,参麦+miR-106a+I/R组NRK-52E细胞miR-106a表达显著升高,细胞活性、Ki67蛋白表达显著降低,细胞凋亡率、Cleaved-caspase-3蛋白表达显著升高(P<0.05).与正常对照组比较,I/R组NRK-52E细胞p-PI3K、p-AKT蛋白表达水平显著降低(P<0.05);与I/R组比较,参麦+I/R组NRK-52E细胞p-PI3K、p-AKT蛋白表达水平显著升高(P<0.05);与参麦+miR-NC+I/R组比较,参麦+miR-106a+I/R组NRK-52E细胞p-PI3K、p-AKT蛋白表达水平显著降低(P<0.05).结论 参麦注射液可促进细胞增殖,抑制细胞凋亡,保护肾小管上皮细胞NRK-52E缺氧-复氧损伤,其机制可能与抑制miR-106a表达进而激活PI3K/AKT信号通路有关.

目的 分析卵巢颗粒细胞瘤的临床资料及CT资料,提高对该疾病的诊断能力.方法 回顾性分析13例经手术病理证实的卵巢颗粒细胞瘤的临床及CT资料.结果 13例患者中11例初发,且均为单个病灶,左侧卵巢5例,右侧卵巢6例,呈圆形或椭圆形.2例患者为复发,1例单发病灶,呈不规则形,1例3个病灶,呈圆形或椭圆形.CT表现初发病例与复发病例相似,多数肿块边缘光滑,与周围组织分界清楚,11个病灶表现为囊实性肿块,2个病灶以实性为主肿块,2个病灶以囊性为主肿块.增强扫描实性部分、囊壁及分隔不同程度强化,3例呈轻度强化,8例呈中度强化,2例呈明显强化.8例患者伴有不同程度子宫体积增大、子宫内膜增厚,3例患者伴有子宫肌瘤,6例患者伴有腹水.结论 卵巢颗粒细胞瘤的临床特点及CT表现具有一定的特征性,但缺乏特异性,综合分析有助于提高该疾病的正确诊断率.

Objective To investigate the mechanism of cellular senescence in ischemia reperfusion injury (IRI)-induced acute kidney injury (AKI) that leads to chronic kidney disease (CKD) in elderly mice.Methods An acute kidney injury model was established in C57B1/6 male mice at ages 8-10 weeks (young group) or 20-24 months (old group) by bilateral IRI.The animals were randomly divided into 4 groups as follows:Young-Sham (n=8),Old-Sham (n=8),Young-IRI (n=8),and Old-IRI groups (n=8).All mice were weighted,and their blood was collected from the tail vein at days 1,3,and 7 after surgery.The mice were killed on day 14 after surgery,and their kidneys were harvested for further analysis.Serum was used for the creatinine test.The changes of the renal tissue morphology and pathology were assessed using hematoxylin-eosin staining and sirius red staining.Immunofluorescence staining of collagen Ⅰ,F4/80,phosphor-histone H3 (p-HH3),and Ki67 were performed to determine the stage of the collagen deposit,macrophage filtration,and cell cycle G2/M arrest.The collagen Ⅰ expression was analyzed using western blot.The expression levels of TNF-α,IL-6,TGF-β,and collagen Ⅰ were determined using real-time PCR.Results Compared with that in the sham group,the serum creatinine levels in both Young-IRI and Old-IR1 groups were obviously increased.The Young-IRI group recovered completely on day 7.The Old-IRI group had higher creatinine levels than the Young-IRI group at each time point.Morphology and pathology analyses revealed that acute injury was repaired in the Young-IRI group,but slight inflammatory cell filtration and collagen deposition were observed in the Old-Sham and Old-IRI groups,respectively.Immunofluorescence staining revealed some F4/80-positive macrophage filtration,collagen Ⅰ deposition,and p-HH3 and Ki67 double-positive nuclear tubular epithelial cells in the Old-Sham group,but considerably more positive results were found in the Old-IRI group.Western blot analysis revealed that collagen Ⅰ expression level was higher in the Old-IRI group than in the Young-IRI group (P ＜ 0.01) and in the Old-Sham group than in the Young-Sham group (P ＜ 0.05).Real-time PCR demonstrated that the mRNA expression levels of cytokines and fibrosis markers,including of TNF-α,IL-6,TGF-β,and collagen Ⅰ,in the Old-Sham and Old-IRI groups were increased as compared with those in the Young-Sham and Young-IRI groups (P ＜ 0.05).Conclusions The levels of kidney inflammation,fibrosis,and cell-cycle arrest are lower in the old mice.After IRI injury,a sustained and ongoing inflammatory reaction is involved and more cells are arrested in the cell cycle G2/M,which inhibit renal repair and promote fibrosis progression.

住院医师规范化培训的全面实施是医学模式转变的重要组成部分.肿瘤外科作为外科学的重要组成部分,在培训和考核规培医师临床技能方面具有重要作用,这对肿瘤外科的带教老师也是个极其重大的考验.本文总结了我科在培养规培医师方面的问题和经验,探讨肿瘤外科规培工作的要点与创新,以提高规培医师在肿瘤外科培训期间的教学质量.

目的 探讨肾小管上皮细胞中可溶性表氧化物水解酶(sEH)在小鼠巨噬细胞极化中的作用.方法 以人近端肾小管上皮细胞系HK-2细胞及小鼠巨噬细胞系RAW264.7细胞为研究对象.将HK-2细胞分为正常对照组、sEH抑制剂组、尿蛋白组及sEH抑制剂联合尿蛋白组;正常对照组细胞不给予任何干预处理;sEH抑制剂组细胞给予1 μmol·L-1 sEH抑制剂;尿蛋白组细胞给予10 g·L-1尿蛋白;sEH抑制剂联合尿蛋白组细胞给予10g· L-1尿蛋白和1 μmol·L-1 sEH抑制剂;各组细胞均培养24 h.将RAW264.7细胞分为A、B、C、D组及干扰素-γ(IFN-γy)阳性对照组、白细胞介素(IL)-4阳性对照组.A、B、C、D组细胞分别加入正常对照组、sEH抑制剂组、尿蛋白组、sEH抑制剂联合尿蛋白组培养24h的HK-2细胞培养基孵育24h;IFN-γ阳性对照组和IL-4阳性对照组细胞分别加入M1型巨噬细胞诱导剂IFN-γ及M2型巨噬细胞诱导剂IL-4.采用Western blot法检测HK-2细胞中sEH蛋白表达,实时荧光定量聚合酶链反应检测HK-2细胞中单核细胞趋化蛋白-1(MCP-1)、IL-6、集落刺激因子-1(CSF-1)、肿瘤坏死因子-α(TNF-α) mRNA表达及RAW264.7细胞中诱导型氮氧化物合酶(iNOS)、IL-6、精氨酸酶-1(Arg-1)及IL-10 mRNA表达.酶联免疫吸附试验检测各组HK-2细胞培养上清液中14,15-环氧二十碳三烯酸(14,15-EET)和14,15-脱氧二十碳三烯酸(14,15-DHET)水平,计算14,15-EET/14,15-DHET比值.结果 正常对照组和sEH抑制剂组HK-2细胞中sEH蛋白表达及MCP-1、IL-6、CSF-1、TNF-α mRNA表达、细胞培养上清液中14,15-EET/14,15-DHET比较差异均无统计学意义(P＞0.05).与正常对照组比较,蛋白尿组HK-2细胞中sEH蛋白及MCP-1、IL-6、CSF-1、TNF-α mRNA表达均显著增加(P＜0.05),细胞培养上清液中14,15-EET/14,15-DHET显著降低(P＜0.05).与尿蛋白组比较,sEH抑制剂联合尿蛋白组HK-2细胞中MCP-1、IL-6、CSF-1及TNF-α mRNA表达显著下降(P＜0.05),培养上清液中14,15-EET/14,15-DHET显著升高(P＜0.05).sEH抑制剂联合尿蛋白组与尿蛋白组HK-2细胞中sEH蛋白表达比较差异无统计学意义(P＞0.05).A组与B组RAW264.7细胞中IL-6、iNOS、Arg-1及IL-10 mRNA表达比较差异均无统计学意义(P＞0.05).与A组比较,C组、IFN-γ阳性对照组RAW264.7细胞中iNOS、IL6 mRNA表达显著增加(P ＜0.05,P ＜0.01);IL-4阳性对照组RAW264.7细胞中Arg-1、IL-10 mRNA表达显著增加(P＜0.05).与C组比较,D组RAW264.7细胞中iNOS、IL-6 mRNA表达显著降低(P＜0.05),Arg-1、IL-10 mRNA表达显著增加(P＜0.05).结论 肾小管上皮细胞中的sEH可上调诱导M1型巨噬细胞极化的细胞因子表达.

Objective To discuss the inhibitory effects of microRNA (miRNA, miR)-143 on the invasion and metastasis of esophageal cancer (EC) cells by downregulating Wnt/β-catenin level.Methods The EC109-H, TE-1 and Het-1A cells were cultured in vitro.Then the cells were randomly divided into experimental group (EC109-H, TE-1)and negative control group (Het-1A);Transwell assay, real-time quantitative polymerase chain reaction(Real-time PCR) and Western blotting were used to detect invasive ability of each group, and the expression of miR-143, Wnt, β-catenin and CD44 related to signaling pathway Wnt/β-catenin in different cell lines.The correlation between miR-143 and regulatory proteins of Wnt/β-catenin was analyzed.The targets related to miR-143 were predicted through online software.The miR-143 inhibitors and mimics were used to change the levels of miR-143 in each group, and CD44-3'untranslated region (3'UTR) carrier and CD44-3'untranslated regions mutation (3'UTRM) mutant carrier were verified.The forecast target was vertified by the double luciferase experiment.Results Of the cell lines, EC109-H had the strongest invasive ability (156.86±0.83, P=0.003).The expression levels of miR-143 in EC109-H cells were the lowest (1.01±0.12,P=0.000).The expression levels of Wnt, β-catenin and CD44 in EC109-H cells which had strongest invasive ability were the highest (0.51±0.11, P=0.005;0.65±0.12, P=0.007;0.80±0.09, P=0.001).Pertinence analysis showed there was a negative correlation between miR-143 and those proteins (rCD44=-0.827,P=0.000;rwnt=-0.907,P=0.017;rβ-catenin=-0.810,P=0.028).We predicted CD44 might be a control target of miR-143.Dual luciferase assays showed that miR-143 could target CD44-3'UTR region (0.32±0.28,P=0.000).Conclusion MiR-143 may regulate the migration and invasion ability of esophageal cancer cells by targeting Wnt/β-catenin signaling pathway.

Objective To investigate the effect and mechanism of soluble epoxide hydrolase inhibitor (sEHI) for NF-κB pathway and cell circle arrest of tubular epithelial cell in unilateral ureteral obstruction (UUO) mice model.Methods Thirty-two healthy C57BL/6 male mice performed UUO surgery to induce renal interstitial fibrosis.Animals were randomly divided into 4 groups:sham group (n=8),sEHI (1 mg· kg-1·d-1) group (n=8),UUO group (n=8) and UUO+sEHI (1 mg· kg-1· d-1) group (n=8).Daily sEHI [1-(1-methylsulfonyl-piperidin-4-yl)-3-(4-trifluoromethoxy-phenyl)-urea,TUPS] or 2％ DMSO was applied to mice by oral gavage from day 1 to day 14 after surgery.All mice were sacrificed at day 14 and kidneys were harvested for further analysis.The changes of renal tissue morphology and pathology were observed by Hematoxylin and eosin (HE) and sirius red staining.The expressions of sEH,nuclear factor κB p65 (NF-κB p65) and IκB were measured by Western blotting.The expressions of TNF-α,IL-1β,MCP-1,IL-6,TGF-β,CTGF,collagen-Ⅳ and α-SMA were analyzed by real-time PCR.Immunofluorescence staining of phospho-histone H3 (p-HH3) and Ki67 was performed to determine the stage of cell cycle G2/M arrest.Results The expression and activity of sEH increased in UUO group (P ＜ 0.05).Administration of sEHI inhibited activity of sEH and infiltration of inflammatory cell in tubular interstitial,as well as attenuated tubular damage and tubular interstitial fibrosis.Western blotting analysis revealed administration of sEHI inhibited up-regulated NF-κB p65 and down-regulated IκB in UUO group (P ＜ 0.05).Real-time PCR demonstrated that administration of sEHI obviously decreased the mRNA expression of cytokines and fibrosis markers,including of TNF-α,IL-1 β,MCP-1,IL-6,TGF-β,CTGF,Collagen-Ⅳ,α-SMA (P ＜ 0.05).Immunofluorescence staining showed that there were much more p-HH3 and Ki67 double positive nuclear tubular epithelial cells and interstitial cells in UUO group,compared with Sham group (P ＜ 0.05).Administration of sEHI reduced the number of double positive nuclear cell only in tubular epithelial cells (P ＜ 0.05),but not in interstitial cells.Conclusions In UUO tubular interstitial fibrosis model,sEHI inhibits the activation of NF-κB pathway by down-regulating p65 and up-regulating IκB and ameliorates the infiltration of inflammatory cells.In addition,sEHI plays anti-fibrosis effect by moderating cell cycle G2/M arrest and reducing the excrete of pro-fibrosis factors of tubular epithelial cells.

Objective To discuss the etiology,clinical features,diagnosis and treatment of congenital pleural effusion in fetuses and neonates in order to improve the understanding of this disease. Methods Six neonate cases with congenital pleural effusion were retrospectively analyzed in neonates who were admitted to the Department of Neonatolo-gy of the First Affiliated Hospital of Zhengzhou University after birth and the etiology,clinical features,diagnosis and re-lated treatment were analyzed based on literature review. Results Among 6 patients with congenital pleural effusion, chylothorax was found in 3 cases,one of whom had chylothorax,chylous abdominal,21 trisomy syndrome and congenital hypothyroidism,and the etiology of the other 3 cases were unknown. Diagnosis mainly was depended on imaging and la-boratory tests. The number of nucleated cells of hydrothorax or ascites were(1 588 - 13 057)× 106 / L,mainly lympho-cytes. Protein qualitative was( + - + + + ),the chylothorax qualitative test was positive in 3 cases. The liver function showed that the total protein and albumin were decreased in varyig degrees. Conservative or surgical treatment was taken according to the situation of the patients. Among 6 patients,3 cases were cured after treatment,the others gave up treat-ment finally. Conclusions The etiology of congenital pleural effusion was different,and often associated with chromo-somal abnormalities or other malformations,ultrasonography was the main method to diagnose congenital pleural effusion and the patients should be dynamically observed or treated during the antepartum or intrapartum period. If the neonates are found to have pleural effusion in prenatal period early diagnosis and treatment is a best choice.

目的：探讨食管癌患者手术前后 Th1/Th2平衡的变化,为恶性肿瘤的免疫治疗提供依据。方法通过酶联免疫法检测食管癌患者手术前后、复发及正常人IFN-γ、IL-2、IL-4、IL-6、IL-10、IL-5的含量水平,分析其含量水平变化的原因。结果食管癌患者术前及复发组的Th1和Th2型细胞因子的含量水平均与正常人差异有显著性(P<0.01),Th1型细胞因子显著下降(P<0.01),而Th2型则显著升高(P<0.01),表明Th1/Th2处于明显的失衡状态。当食管癌患者手术之后,Th1型细胞因子含量则上升(P<0.01),而Th2型细胞因子含量则下降(P<0.01)。结论食管癌患者手术之后有利于T细胞亚群的恢复和促使手术前Th1/Th2型细胞因子的偏移得到一定的纠正,改善机体的免疫功能。

Aim: To detect the levels of serum lipid metabolic and inflammation mediator in rats intratracheally instilled with zinc oxide nanoparticles.Methods:A total of 40 male Wistar rats were randomly divided into four groups:the control group,and zinc oxide nanoparticles low,medium,high dose groups. The rats of zinc oxide nanoparticles low,medium,high dose groups were instilled with 1.25,2.50,5.00 mg/kg zinc oxide nanoparticles,respectively.And the control group were given PBS. Rats were intratracheally instilled with zinc oxide nanoparticles once a week. After 12 weeks, body weight and the levels of serum TC, LDL, HDL, TNF-α and HO-1 were determined.Results:With exposure continued, rats in the experimental groups presented rough fur, inactive, poorer mental state, and slow growth.The levels of serum HDL,LDL,TNF-α,HO-1 and TC among the 4 groups were different(F=32.410,34.600,23.957,38.519,32.652,P<0.001). Levels of serum TC,TNF-α,LDL and HO-1 in zinc oxide nanoparticles medium,high dose groups were higher than those in the control group (P<0.05 ).Levels of serum HDL in zinc oxide nanoparticles low,medium,high dose groups were lower than those in the control group(P<0.05).Conclusion:Intratracheal instillation of zinc oxide nanoparticles could induce lipid metabolic disorders and inflammation mediator release,and these changes in serum have certain effects on atherosclerosis.

Objective To discuss the ultrasonographic feature and prognosis of the fetal dacryocystocele and its correlation.Methods The ultrasonographic feature of 51 patients diagnosed as dacryocystocele prenatally by sonography were retrospectively analyzed.All fetus were followed up to induced labor or 6 months after birth.The correlation between the size and the gestational age were analyzed by correlation analysis method and compared the fetal prognosis adopt U test.Results The incidence of dacryocystocele diagnosed prenatally by ultrasound was about 0.47‰.The diagnosis was established by the detection of a cystic lesion in relation to the medial and inferior aspects of the fetal orbit.Unilateral dacryocystoceles were more often found than bilateral,and left more than right.The maximum diameter range from 2.0 mm to 12.0 mm,average (5.9 ± 2.2)mm.There is no correlation between cyst size and gestational age.Prognosis of isolated nasolacrimal duct cyst is good.Conclusions The fetal dacryocystocele have typical ultrasonographic feature,Ultrasound is a rapid and reliable method which can be used for diagnosis of dacryocystocele prenatally and follow-up after birth.

目的探讨腺苷脱氨酶(ADA)检测在脑积液(CSF)中的诊断意义。方法采用比色法检测3组病例(结核性脑膜炎组40例,病毒性脑膜炎组43例,化脓性脑膜炎组40例)CSF中ADA活性水平。结果研究发现结核性脑膜炎组CSF中ADA活性水平明显升高,与病毒性脑膜炎组和化脓性脑膜炎组比较有显著性差异(P<0.05),并且发现病毒性脑膜炎组与化脓性脑膜炎组CSF中ADA活性水平也有显著性差异(P<0.05)。结论说明检测CSF中ADA活性水平对于鉴别结核性脑膜炎、病毒性脑膜炎和化脓性脑膜炎有一定的诊断价值。

Aim: To investigate the effects of IGF-ⅠR antisense oligonucleotide(ASODN) on protein expression of IGF-ⅠR in human lung cancer NCI-H460 cell.Methods:Three IGF-ⅠR ASODN sequences were designed and synthesized.The NCI-H460 cells were transfected by IGF-ⅠR ASODN,N-ODN.The expression of IGF-ⅠR protein was detected by immunocytochemistry SP method.Results: The positive expression of IGF-ⅠR protein was examined and could be inhibited by ASODNs.There was no significant difference among 3 ASODN groups(P0.05),but there was significant difference between ASODNs and N-ODN or non-transfected group(P0.05).Conclusion: The expression of IGF-ⅠR protein in NCI-H460 cells could be inhibited by ASODNs.

Aim: To investigate the expression of Caveolin-1 and EGFR proteins in colorectal carcinoma tissue. Methods:The expression of Caveolin-1 and EGFR proteins in 126 cases of colorectal carcinoma tissues,35 para-carcinoma normal mucosa tissues were detected using immunohistochemical method. Results: The positive rates of Caveolin-1 and EGFR in colorectal carcinoma tissue were 30.16%(38/126) and 61.11%(77/126), respectively, which significantly differed from those in normal mucosa tissues 85.17%(30/35) and 8.57%(3/35)(P0.001 ). The expression levels of the two proteins in colorectal carcinoma tissue were not related to the histopathologic type(P0.05 ), but correlated with malignant degrees,lymph node metastasis and clinical stages (P0.05).The expression of Caveolin-1 in colorectal carcinoma tissue was negatively correlated with that of EGFR(P0.05).Conclusion: The expression levels of Caveolin-1 and EGFR proteins are remarkably related to the progression and tumor metastasis.

Aim: To investigate the influence of small interfering RNA(siRNA) on the expression of cathepsin B(CB) gene in human esophageal carcinoma EC9706 cells. Methods: Specific siRNA of CB gene was obtained by in vitro transcription. EC9706 cells were transfected by specific siRNA at different concentrations (10 μmol/L, 15 μmol/L, 20 μmol/L) for 24 h, 48 h, or 72 h, respectively. The non-specific siRNA group, empty liposome group, and normal control group were used as control. The expression of CB mRNA and protein was detected using in situ hybridization and immunohistochemistry. Results: Compared with control groups, there was a significant decrease in the CB protein and mRNA level in specific siRNA transfection groups, and the inhibition effect was in a concentration dependent manner. It was observed that some EC9706 cells had morphological changes after the transfection.Conclusion: Specific siRNA can effectively inhibit the expression of CB gene in EC9706 cells.

肿瘤和结核是导致胸水的常见的原因,为探讨结核性和癌性胸腔积液中腺苷脱氨酶(ADA)、癌胚抗原(CEA)和一氧化氮(NO)的变化及鉴别诊断中的价值,我们测定30例结核性胸腔积液(结核组)和30例癌性胸腔积液(癌性组)中的ADA、CEA及NO.现报告如下:

Objective To compare emulsion agglutination turbidimetric method and speed scattering turbidimetric method for determining serum level of high-sensitivity C-reactive protein(hsCRP)in cerebrovascular disease patients and the clinical relevance.Methods Both methods mentioned above were used simultaneously fordetermining serum level of hsCRP in 59example specimens collected fromcontrol group and acute cerebrovascular disease group.Results The positive rate of emulsion agglutination turbidimetric method and speed scattering turbidimetric method was 54.24% and 47.46% respectively;there was no significant difference(P>0.05) between the two methods;serum level of hsCRP was higher in acute cerebrovascular disease group than that in normal control group(P<0.01).Conclusion The sensitivity is consistent between the two methods in examining hsCRP;there is explicit significance in hs CRP determination to acute cerebrovascular disease.