Background: Plasmablastic lymphoma (PBL) is a rare but aggressive B-cell lymphoma subtype with poor prognosis. Knowledge about the etiology, clinicopathologic and molecular features, and outcomes of PBL is limited. This study aimed to examine the clinicopathologic characteristics, therapeutic approaches, and clinical outcomes of PBL patients in a Chinese population. Methods: A total of 102 PBL patients were recruited from three cancer centers. The pathologic features and clinical outcomes of 56 patients with available treatment details and follow-up data were reviewed and analyzed. RNA sequencing was performed in 6 PBL and 11 diffuse large B-cell lymphoma (DLBCL) patients. Results: Most patients in our cohort were male (n = 36, 64.3%), and 35 patients presented with Ann Arbor stage I/II disease at diagnosis. All these patients showed negative findings for human immunodeficiency virus, and the vast majority of patients in our cohort were immunocompetent. Lymph nodes (n = 13, 23.2%) and gastrointestinal tract (n = 10, 17.9%) were the most commonly involved site at presentation. Post-treatment complete remission (CR) was the only prognostic factor affecting overall survival (OS) and progression-free survival (PFS) in the multivariate analysis. RNA-seq demonstrated that B-cell receptor (BCR), T-cell receptor (TCR), P53, calcium signaling, and Wnt signaling pathways were significantly downregulated in PBLs compared with GCB (or non-GCB) DLBCLs. Conclusions: In this multicenter study in the Chinese population, PBL mainly occurred in immunocompetent individuals and most patients present with early-stage disease at diagnosis. Post-treatment CR was an important prognostic factor affecting OS and PFS. RNA-seq showed that the B-cell receptor (BCR), P53, calcium signaling, cell adhesion molecules, and Wnt signaling pathways significantly differed between PBL and GCB (or non-GCB) DLBCL, which provided theoretical basis for its pathogenesis and future treatment.

Objective To investigate the clinical and pathologic feature, immunophenotype, treatment and prognosis of plasmablastic lymphoma patients. Methods The clinical data of 5 plasmablastic lymphoma patients who were admitted into the Second Xiangya Hospital of Central South University and Hunan Cancer Hospital between 2010 and 2016 was collected. We analyzed the clinical and pathologic fea-tures, immunophenotype, treatment and prognosis of these five cases, followed by a review of related literature in China and abroad. Results A1l of these 5 patients were diagnosed as HIV-negative PBL, 4 of 5 patients were males, and only one was female. The median age was 53.8(42~66) years old. Four had B symptoms. All the 5 patients showed the morphologicalfeature of immunoblasts but plasma cell immu-nophenotype. Three patients received a thracycline based chemotherapy, two patients received bortezomib. One patient received GEMOX (Gemcitabine, Oxaliplatin) and involved field radiotherapy. Only one patient underwent right hemicolectomy. Three patients achieved dura-ble complete response and are still alive with survival times of 24, 10, 23 months respectively. Two patients had progressive disease and died 3 and 6 months after admission. Conclusion We conclude that plasmablastic lymphoma is a rare lymphoma which resembles immunoblasts morphologically but showed plasma cell immunophenotype, and this tumor progress rapidly clinically and have poor prognosis, the treatment remains a challenge, more effective treatment regimen is urged to be investigated.

Objective To detect the expressions ofβ?catenin, RIPK4, CDK8 and assess the relationship between the expres?sions of above proteins in mantle cell lymphoma( MCL) with their clinicopathological characteristics and prognosis. Methods Strept Avidin?Biotin Complex immunohistochemistry was used to detect the expression ofβ?catenin, RIPK4 and CDK8 in 30 samples of MCL and 16 samples of lymphadenitis. All MCL patients were followed?up. The expressions of β?catenin, RIPK4 and CDK8 in MCL and lymphadentis tissues were compared. The correlation of above proteins and the relationship between the expression of them in MCL with their clinicopathological characteristics and prognosis were analyzed. Results Positive expression rates of β?catenin, RIPK4 and CDK8 in MCL tissues were 55?3%, 63?3% and 60?0%, which were higher than those of lymphadenitis samples( P<0?05) . There was a positive correlation among the expression of β?catenin, RIPK4 and CDK8( r=0?600,r=0?675,r=0?367,P<0?05) . The expression of β?catenin, RIPK4 and CDK8 had no significant correlation with the gender, age, MIPI score, the level of β2?microglobulin, B symptoms and clinical stage(P>0?05). The median overall survival (OS) of MCL patients was 21 months. MIPI score of high risk group, the positive expression of CDK8 were independent predictors of OS. Conclusion There were high expression rate ofβ?catenin, RIPK4 and CDK8 proteins in MCL. There was a positive correlation among the expression ofβ?catenin, RIPK4 and CDK8. The positive expression of CDK8 indicated a poor prognosis of MCL.

Objective To investigate HPV infection rate,subtype distribution and HPV infection status in different ages among gynecological patients,and to provide the basic data for the prevention and control of HPV infection. Methods Cervical tissue specimens from 9,266 gynecological outpatients in Xiangtan Maternal and Child Health Hospital from October 2009 to July 2011 were examined with flow-through hybridization and gene chip technique(cape medical system) to determine the 21 HPV genotypes.Excel 2007 and SPSS17.0 software were used to establish database and analyze the data.The Chi-square test was used in the comparison of the HPV infection rates among different age groups. Results Of the 9,266 specimens,HPV was detected in 1,952 specimens,with a positive rate of 21.07%(1,952/9,334).The high-risk HPV infection rate was 18.55% (1,719/9,266),the low-risk HPV infection was 2.51%(233/9,266),single subtype infection rate was 15.55%(1,441/9,266),and the infection rate of multiple subtypes was 5.51%(511/9,266).Of all the subtypes in this study,HPV52,HPV16,and HPV58 ranked the top three HPV infection and with the single and double infection primarily,with the constituent ratio of the single and multiple infection,the patients infected high risk subtypes were statistically significant different from those infected with low-risk subtypes(χ2=68.37,P0.01).The difference was statistically significant in the HPV infection rate among different ages(χ2=28.27,P0.01).The constituent ratio of the HPV infections from different age groups was significantly different in the distribution of the high-risk and low-risk HPV subtypes(χ2=32.87,P0.01) and the HPV single and complex infection (χ2=48.49,P0.01).The single and high-risk HPV infection was the main infection in different age groups.In the age group of less than 25 years and more than 50 years,the constituent ratios of the high-risk and low-risk co-infection were higher than other groups. Conclusions HPV infection rate of gynecological outpatients in Xiangtan Maternal and Child Health Hospital was at high level.The most common HPV infection subtypes were HPV52,HPV16,and HPV58.The HPV infection varied in different age groups,and increased with the age increasing.The distribution of high-risk and low-risk HPV infection as well as the single and complex infection was different among various ages.In the age groups of less than 25 years and more than 50 years,the constituent ratios of the high-risk and low-risk co-infection were higher than those of infection patients aged 25～50 years.

【Objective】 To learn the HPV infection rate,subtype distribution and HPV infection status in different age among gynecological clinic patients,so as to provide a scientific basis for the prevention and control of HPV infection.【Methods】 Cervical tissue specimens from 11 461 gynecological clinic patients were examined with Flow-through Hybridization and gene chip technique(cape medical system) to determine the 21 HPV genotypes.Making use of the Excel 2007 and SPSS 17.0 software to establish database and analyze data,the Chi-square test was used in the comparison of the HPV infection rate between different age groups.【Results】 ① Of the 11 461 specimens,HPV was detected in 5 096 specimens with a positive rate of 44.45%(5096/11461),the high-risk HPV infection rate was 40.54%(4646/11461),the low-risk HPV infection was 4.26%(488/11461),single subtype infection rate was30.89%(3540/11461),and complex subtypes infection rate was 14.00%(1604/11461).② Of all the subtypes in this study,HPV16 was highest infection rate of subtype(15.55%),and with the single and double infection primarily.③ The difference was significant in the HPV infection rate between different ages(χ2=77.03,P <0.01).Along with the increasing age,HPV infection rates increase gradually(χ2=76.67,P <0.01),the constituent ratio of the HPV infections from different age group was significant different on the distribution of the high-risk and low-risk HPV subtypes(χ2=121.70,P <0.01),and the HPV single and complex infection(χ2=121.70,P <0.01).the single and high-risk HPV infection was the main infection in different age groups.In the age group of less than 30 years and more than 60 years,the constituent ratio of the high-risk and low-risk co-infection is higher than other groups.【Conclusion】 HPV infection rates in our hospital gynecological clinic was at high level,the highest subtype in the HPV infection rate was the HPV16.The HPV infection varied in different age groups,and increased with the age increasing.The distribution of high-risk and low-risk HPV infection as well as the single and complex infection was different between various ages.In the age group of less than 30 years and more than 60 years,the constituent ratio of the high-risk and low-risk co-infection is higher than 30～60 years old infection.

Objective To review the experiences of the revascularization in the surgery of mediastinal tumor.Methods 76 patients with mediastinal tumor were resected with vascular reconstruction including 46 patients of vascular replacement and 30 patients of vascular angioplasty.It invaded the superior vena cava in 22 patients,left or right innominate vein in 16 patients,the superior vena cava accompanied with left(or right) innominate vein in 34 patients and only aortic adventitia in 4 patients.70 patients were subjected to radical excision and 6 patients to palliative excision.Results There was no case of perioperative death.The blood block time of the superior vena cava was(18.0±5.3) minutes.And the block time of the innominate vein was(16.5±4.2) minutes.All the cases were satisfied with the quality of life during the follow-up for 12 to 26 months.Conclusion Operation is the first choice for mediastinal tumor patients whose superior vena cava and its branches are invaded without serious involvement of body system.

Aim This study was designed to identify the T-type calcium channel blocker Mibefradil as a cardioplegic agent in terms of its efficacy in immature cardioprotection. Methods By a Langendorff model, 32 hearts of 14- to 21-day-old New Zealand rabbits underwent 90 min of global hypothermic (15℃) ischemia protected with a different single dose of hypothermic (4℃) cardioplegia(group ST:St.Thomas solution; group SV:St.Thomas solution combined with 0.1 μmol/L Verapamil; group SM1:St.Thomas solution combined with 0.1 μmol/L Mibefradil;group SM2:St.Thomas solution combined with 1 μmol/L Mibefradil). The coronary flux, percent recovery of the cardiac function, LDH release, myocardium MDA, and myocardium ionized calcium were compared. Results There are no significant difference in coronary flux between the groups. Verapamil provided significantly the worst postreperfused percentage recoveryof the cardiac function (developed pressure: 0.70±0.23, dp/dt: 0.72±0.23, and -dp/dt: 0.67±0.25) than the other groups (P0.05). There are no significant difference in the cardiac function between others groups. The higher concentration Mibefradil (1 μmol/L) showed a significant reduction of LDH release (6.6±2.2 IU/L vs 9.3±3.2 IU/L)in the coronary flow, and lower myocardium MDA (1.14±0.24 μmol/g vs 1.64±0.39 μmol/g) and ionized calcium (0.147±0.020 mg/g vs 0.184±0.021 mg/g) than group ST (P0.05). Verapamil showed a highest LDH release (12.2±2.7 IU/L) in the coronary flow and myocardium ionized calcium (0.225±0.041 mg/g)(P 0.05 ). Conclusion St.Thomas solution combined with Mibefradil provided better protection during ischemia-reperfusion in the immature rabbit heart than St.Thomas' solution, whereas St.Thomas' solution combined with Verapamil showed the worst protective effects in immature hearts