Severe infections and a complicated healing process caused by multidrug-resistant bacteria in skin wounds have urgently driven the development of novel antibacterial agents. In this study, bimetallic copper-cobalt nanoclusters (CuCo NCs) were successfully synthesized via a one-pot aqueous co-reduction method and evaluated as a potential antimicrobial platform for wound infection treatment. The peroxidase-like (POD-like) activity of CuCo NCs catalyzes H2O2 to generate hydroxyl radicals (•OH), inducing membrane disruption of bacteria. Consequently, the CuCo NCs exhibited potent and broad-spectrum antibacterial activity against both Gram-negative and Gram-positive bacteria, including Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), with minimum inhibitory concentrations (MICs) of 45.0 μg/mL and 11.3 μg/mL, and minimum bactericidal concentrations (MBCs) of 90.0 μg/mL and 22.5 μg/mL, respectively, along with favorable biocompatibility. In a mouse model of dorsal skin wound infection, treatment with the CuCo NCs-loaded wound dressing significantly accelerated wound closure and promoted tissue regeneration. This study offers valuable insights into the rational design of bimetallic CuCo nanoclusters, which may shed light on the development of effective strategies for combating wound-associated infections and advancing clinical wound management.

Adsorption and biodegradation are two important means to remove the pollutants from the environment, but how to combine them and improve the catalytic performance and stability of free enzyme are facing great challenges. Herein, lipase from Candida rugosa (CRL) was immobilized into bimetallic ZnCo-MOF by biomineralization, which not only significantly improved the catalytic activity and stability of CRL but also endowed it with excellent reusability. Furthermore, CRL@ZnCo-MOF established a synergetic system of combined adsorption and enzymatic degradation for the sustainable removal of dibutyl phthalate (DBP) in actual water environment. The adsorption of DBP by CRL@ZnCo-MOF with mesoporous structure is mainly carried out by the monolayer adsorption via chemical adsorption, wherein the interaction between them is predominantly mediated by the hydrogen bonds and coordination bonds of MOF and DBP. Moreover, due to the ester bond cleavage ability of CRL, the DBP was degradated to less toxic monobutyl phthalate (MBP) and phthalic acid (PA) by CRL@ZnCo-MOF. Therefore, this study provides new insights into the development of novel approaches for the treatment of pollutants using enzyme@MOF biocomposite through the integration of adsorption-biodegradation effect.

Total antioxidant capacity (TAC) is a critical indicator of a food's ability to scavenge reactive oxygen species (ROS), alleviate systemic inflammation, and maintain redox homeostasis. Rapid and accurate TAC quantification is therefore essential for guiding dietary choices and evaluating food quality. In this study, we developed a dual-mode colorimetric platform combining a conventional solution-based assay with a gel-based system, leveraging the high oxidase-like activity of a Cu single-atom nanozyme (Cu SAzymes). The Michaelis-Menten constant (Km) of Cu SAzymes is low at 1.26 mM, while demonstrating an exceptionally high specific activity of 72.46 U/mg. These performance metrics significantly surpass those of most synthetic nanozymes reported to date. Thanks to the high oxidase-like activity of Cu SAzymes, this platform enables precise, rapid, and efficient TAC measurement in various fruit juices and tea products, achieving a remarkable low detection limit of 1.59 mu M for ascorbic acid detection. Additionally, through comprehensive analysis of TAC during black tea fermentation, this research establishes a novel framework for evaluating fermentation degree, thereby contributing valuable insights to the digital monitoring of black tea fermentation processes.

We demonstrate that precise control of the synthetic pH enables the selective synthesis of either pro-oxidant Cu nanoclusters (CuNCs) or antioxidant CuNCs. Notably, modulating the growth temperature of CuNCs can switch their catalytic activity from a pro-oxidant to antioxidant nanozyme. This tunability may make it possible to remotely control the catalytic activities of Cu nanozymes.

Protein-protected metal nanomaterials are becoming the most promising fluorescent nanomaterials for biosensing, bioimaging, and therapeutic applications due to their obvious fluorescent molecular properties, favorable biocompatibility and excellent physicochemical properties. Herein, we pioneeringly prepared a cellulase protected fluorescent gold nanoclusters (Cel-Au NCs) exhibiting red fluorescence under the excitation wavelength of 560 nm via a facile and green one-step method. Based on the fluorescence turn-off mechanism, the Cel-Au NCs were used as a biosensor for specificity determination of ascorbic acid (AA) at the emission of 680 nm, which exhibited satisfactory linearity over the range of 10–400 µM and the detection limit of 2.5 µM. Further, the actual sample application of the Au NCs was successfully established by evaluating AA in serum with good recoveries of 98.76%–104.83%. Additionally, the bacteria, including gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus) and gram-negative bacteria (Escherichia coli), were obviously stained by Cel-Au NCs with strong red emission. Thereby, as dual-functional nanoclusters, the prepared Cel-Au NCs have been proven to be an excellent fluorescent bioprobe for the detection of AA and bacterial labeling in medical diagnosis and human health maintenance.

Luminescent Cu@Cu2S nanozymes have been prepared by a one-pot method, displaying high peroxidase-like and oxidase-like activity. Fluorescence images show that these nanozymes adhere to the surface of the bacterial cell and scanning electron microscopy reveals that the nanomaterials cause folding and collapse of the bacterial surface, resulting in bacterial death.

Small-sized semiconducting polymer dots (Pdots) provide better tissue and subcellular penetration while minimizing unspecific interactions, and make the fast clearance of Pdots from human bodies possible by urinary excretion. We employ a powerful and scalable technology, flash nanoprecipitation, to prepare Pdots with small sizes (hydrodynamic diameters ∼10 nm).

The fluorescent graphene quantum dots (GQDs) provide an amusing nanoplatform, which integrates unique optical properties of quantum dots and prominent electronic properties of carbon materials.

In this paper, we report a simple and low-energy strategy for the preparation of silver decorated polymer colloids. The reported constrained-volume synthesis integrates polymer nanoprecipitation and metal deposition in a one-step continuous-flow fashion. The deposition of Ag nanoparticles (NPs) associated with different optical properties is easily controlled by varying processing parameters. The as-synthesized colloidal NPs have good antimicrobial properties. In addition, the sensitivity of the colloids towards hydrogen peroxide exhibits a linear response over a wide concentration range with a detection limit of 0.03 μM.

Based on a competition assay approach, a new highly sensitive method for real-time detection of PPase activity using Li+-doped NaYF4:Yb,Er upconversion red luminescent nanoparticles was developed.

Efficient treatment of organic pollutants in water by a facile and green technique is a great challenge for environmental remediation. In this study, we report a simple and low-energy strategy for catalytic reduction of organic pollutants in water by continuous-flow flash nanoprecipitation. The one-step processing technique integrates rapid metal@polymer nanoparticle production and catalytic reaction in a continuous-flow fashion. Such a concept is successfully demonstrated for simultaneous formation of Au@polymer nanospheres and catalytic reduction of organic pollutants (e.g., methylene blue and 4-nitrophenol) in water. Furthermore, the catalytic reaction rate could be easily tuned by varying the processing parameters (e.g., feeding concentration). The activity of the nanocatalyst was demonstrated in five recycles without any detectable loss. The characteristics of continuous-flow mode make the one-step process scalable, promising processing methodology for wastewater treatment.

A new comprehensive experiment- synthesis and characterization of graphene quantum dots was recommended,and its goals,principles,instruments and agents,procedures,and the issues that need to pay attention to in the experiments were studied. The experiment was based on the focus of chemistry,material science,and biology,and covered many experimental skills that college students learned in basic chemistry experiment,such as preparation of compounds,component analysis,and characterization by instrumentals. This experiment had a easily synthetic method,all- round characterization method,modular contents,and flexible scheduling,so it can be as the comprehensive experiment course for the students in chemistry and chemical engineering major.

In this paper, a simple and sensitive photoluminescence method is developed for the hydroquinone quantitation by using graphene quantum dots which simultaneously serve as a peroxidase-mimicking catalyst and a photoluminescence indicator. In the presence of dissolved oxygen, graphene quantum dots with intrinsic peroxidase-mimicking catalytic activity can catalyze the oxidation of hydroquinone to produce p-benzoquinone, an intermediate, which can efficiently quench graphene quantum dots' photoluminescence. Based on this effect, a novel fluorescent platform is proposed for the sensing of hydroquinone, and the detection limit of 5 nM is found.

A sandwich chemiluminescence (CL) immunosensor for sensitive detection of human leptin was developed with a multiple signal amplification strategy from catalytic hemin/G-quadruplex DNAzymes and functional superparamagnetic nanocomposites. To construct this sensing platform, core-shell structural Fe3O4/polydopamine (PD)/Au superparamagnetic nanocomposites were synthesized by an in situ method with PD as a substrate adhesive. These nanocomposites were further characterized by scanning electron microscopy, transmission electronic microscopy, energy-dispersive spectroscopy, Fourier transform infrared spectrum and a vibrating sample magnetometer at room temperature. In this immunosensor, the monoclonal anti-human leptin antibody (capture antibody) was bound to the Fe3O4/PD/Au nanocomposites. Human leptin, biotinylated detection antibodies and streptavidin-DNAzymes were successively combined the above-mentioned nanocomposites to form sandwich-type immunocomplex through specific interactions. The magnetic particles loaded with the immunocomplex were separated by an external magnet, and the DNAzymes in the immunocomplex greatly enhanced the CL emission of the luminol-H2O2 system. The immunosensor exhibited a high sensitivity, a good specificity, and a wide linear range for human leptin detection from 1.0 pg mL(-1) to 8.0 x 10(2) pg mL(-1) with a low detection limit of 0.3 pg mL(-1). This sensor is one of the most sensitive methods for leptin detection due to the highly efficient catalysis of the DNAzymes and analyte enrichment on magnetic capture. (C) 2015 Elsevier B.V. All rights reserved.

Uniform octahedral zinc tetraphenylporphyrin (ZnTPP) microcrystals were obtained in a large scale by a facile, fast and surfactant-free process at room temperature. The effects of synthetic parameters such as solvent and concentration on the formation and morphology of ZnTPP were investigated. Furthermore, by varying the reaction time, both the morphology and the size of the ZnTPP microcrystals can be controlled.

In this work, we reported a sensitive chemiluminescent immunosensor for the detection of human leptin by using hemin/G-quadruplex DNAzymes to amplify detection signal. In this sensing system, the primary antibody (anti-human leptin) was firstly bound to the 96-well plates, and human leptin and biotinylated secondary antibody were successively combined to form sandwich-type immune complex through specific interactions. Then streptavidin labeled with hemin/G-quadruplex DNAzymes was assembled to the sandwich-type immunocomplex by streptavidin-biotin interaction. The DNAzymes exhibited an excellent catalytic activity to the chemiluminescent reaction of luminol with hydrogen peroxide in strong alkaline solution, leading to significant enhancement in response signal. Under the optimum conditions, the proposed immunosensor showed high sensitivity and selectivity with a low detection limit of 1.9 pg mL(-1) and a wide linear response range of human leptin from 10 to 1000 pg mL(-1). The immunosensor was used to detect human leptin in serum, and the results were in good agreement with the data obtained by conventional ELISA method.

In the present work, a highly sensitive and specific fluorescent biosensor for blood glucose monitoring is developed based on hemin-functionalized graphene quantum dots (GQDs) and glucose oxidase (GOx) system. The GQDs which are simply prepared by pyrolyzing citric acid exhibit strong fluorescence and good water-solubility. Due to the noncovalent assembly between hemin and GQDs, the addition of hemin can make hydrogen peroxide (H2O2) to destroy the passivated surface of GQDs, leading to significant fluorescence quenching of GQDs. Based on this effect, a novel fluorescent platform is proposed for the sensing of glucose. Under the optimized conditions, the linear range of glucose is from 9 to 300μM, and the limit of detection is 0.1μM. As unique properties of GQDs, the proposed biosensor is green, simple, cost-efficient, and it is successfully applied to the determination of glucose in human serum. In addition, the proposed method provides a new pathway to further design the biosensors based on the assembly of GQDs with hemin for detection of biomolecules.

应用简单溶液法制备出多棱梭形和球形两种形貌的CuO微纳米材料,用XRD对产品进行表征,用SEM,TEM研究产物的形貌和大小。并研究了两种形貌的CuO微纳米材料对罗丹明B的光催化降解性能,发现这两种形貌CuO微纳米材料对罗丹明B都有良好的光催化降解性能。

本文利用C60与ICl反应合成C60Cl6,经红外吸收光谱仪、紫外-可见吸收光谱仪表征证实;利用AAO模板（氧化铝模板）法成功合成出C60Cl6纳米管,经透射电镜、扫描电镜表征。该法合成出形貌和尺寸可控一维纳米管。将为C60Cl6纳米管的器件化研究提供一定的基础。

A new electrochemical biosensor for sequence-specific target DNA detection was prepared with a multi-walled carbon nanotubes and gold nanoparticles composite film modified electrode.Differential pulse voltammetry was used to monitor DNA hybridization by measuring the electrochemical signals of [Ru(NH3)6]3+ bound to double-stranded DNA.This DNA biosensor can detect the target DNA quantitatively in the range of 1.0 × 10-12-1.0 × 10-7 mol/L,with a detection limit of 3.5 × 10-13 mol/L(S/N=3).In addition,the DNA biosensor exhibits excellent selectivity,even for single-mismatched DNA detection.