目的 研究高压氧治疗颅内破裂动脉瘤血管痉挛对神经、血管功能的影响.方法 选取颅内破裂动脉瘤血管痉挛患者104例,参考随机数字表法,按照1:1将纳入患者随机分为观察组、对照组,每组各52例.观察组行高压氧治疗、对照组采用尼莫地平注射液治疗.观察和比较2组患者治疗前后经颅多普勒超声(TCD)结果、和肽素水平、超氧化物歧化酶(SOD)、丙二醛(MDA)含量.结果 观察组临床治疗显效率、总有效率,高于对照组显效率、总有效率,差异有统计学意义(P<0.05).治疗结束,观察组患者大脑中动脉平均血流速度、搏动指数及阻力指数均低于对照组(P<0.05).治疗结束,观察组患者和肽素水平、MDA含量低于对照组,SOD含量高于对照组,差异均有统计学意义(P<0.05).结论 高压氧治疗颅内破裂动脉瘤血管痉挛效果显著,能减轻血管痉挛对神经及血管功能的损伤,改善预后,值得推广使用.

目的 研究在小儿急性病毒性心肌炎患者的临床治疗中实施磷酸肌酸与1,6-二磷酸果糖治疗的疗效.方法 选取2010年3月—2018年7月安阳市妇幼保健院接诊的70例小儿急性病毒性心肌炎患者,按治疗措施差异分为2组,观察组给予磷酸肌酸治疗;对照组1,6-二磷酸果糖治疗,对比分析治疗疗效、治疗后心肌酶标志物恢复正常值时间和心脏射血分数EF、短轴缩短率FS改善情况.结果 观察组疗效为97.1％,对照组疗效为82.8％(P＜0.05).治疗后观察组 CK(9.88±3.33)t/d,HBDH(11.45±4.56)t/d,CK-MB(10.23±4.23)t/d;EF(60.56±7.55),FS(33.23±4.55);观察组心肌酶标志物恢复正常时间明显较对照组短,治疗后EF、FS指标明显较对照组高(P＜0.05).结论 磷酸肌酸治疗小儿急性病毒性心肌炎疗效显著,值得推广.

目的 探讨帕金森病(PD)病人血清微小RNA-543-3p(miR-543-3p)、微小RNA-124(miR-124)表达水平与胰岛素样生长因子1(IGF-1)、脑源性神经营养因子(BDNF)、神经生长因子(NGF)水平的相关性.方法 收集2017年5月—2018年5月本院收治的PD病人80例为PD组,根据Hoehn-Yahr分级标准将PD病人分为1期16例,2期24例,3期13例,4期15例,5期12例,根据帕金森评定量表(UPDRS)评分将PD病人分为轻度27例,中度31例,重度22例,通过蒙特利尔认知评估量表(MoCA)评估认知功能并将病人分为轻度认知功能障碍(PD-MCI)33例与无认知功能障碍(PD-NCI)47例,同时选取50名健康体检者为对照组.采用实时荧光定量聚合酶链反应(qRT-PCR)检测miR-543-3p、miR-124的表达水平;采用酶联免疫吸附试验(ELISA)检测血清IGF-1、BDNF、NGF水平;采用Pearson法分析PD病人血清miR-543-3p、miR-124与IGF-1、BDNF、NGF水平的相关性.结果 与对照组比较,PD组血清miR-543-3p表达水平明显升高(P<0.05),miR-124表达水平明显降低(P<0.05);与1期、2期比较,3期、4期、5期PD病人血清miR-543-3p的表达水平明显升高(P<0.05),miR-124表达水平明显降低(P<0.05);与轻度组比较,中度组、重度组病人血清miR-543-3p的表达水平明显升高(P<0.05),miR-124表达水平明显降低(P<0.05);与对照组比较,PD组血清IGF-1、BDNF、NGF水平明显降低(P<0.05);miR-543-3p与IGF-1、BDNF、NGF呈负相关(P<0.05),miR-124与IGF-1、BDNF、NGF呈正相关(P<0.05);与PD-NCI组比较,PD-MCI组血清miR-543-3p表达水平明显升高(P<0.05),miR-124表达水平明显降低(P<0.05).结论 PD病人血清miR-543-3p表达上调、miR-124表达下调,二者均与IGF-1、BDNF、NGF水平密切相关.

目的 探究加温加湿高流量鼻导管吸氧联合枸橼酸咖啡因治疗早产儿原发性呼吸暂停的临床疗效及安全性.方法 选取新生儿重症监护病房收治的呼吸暂停新生儿110例,按照随机数字表法分为对照组和观察组各55例,对照组给予枸橼酸咖啡因注射液+经鼻持续气道正压通气治疗;观察组给予枸橼酸咖啡因注射液+加温加湿高流量鼻导管吸氧治疗.观察对比两组临床疗效、安全性及患儿舒适度、家属接受度.结果 观察组呼吸暂停消失时间、有创通气比率、治疗72h内呼吸暂停次数和总用氧时间均低于对照组(P＜0.05);观察组胃潴留、心率加快、鼻损伤、气胸和支气管肺发育不良等不良反应情况均低于对照组(P＜0.05);观察组患儿舒适度、家属接受度均高于对照组(P＜0.05).结论 加温加湿高流量鼻导管吸氧联合枸橼酸咖啡因治疗早产儿原发性呼吸暂停疗效显著,可降低不良反应发生率,提高患儿舒适度、家属接受度.

目的 探讨miR-142-5p/APLN对癫痫发作后神经元损伤的影响及其调控机制.方法 构建大鼠海马神经元癫痫模型,正常神经元细胞作为正常对照组,以此神经元细胞为癫痫细胞模型组.取癫痫大鼠神经细胞分为anti-miR-142-5p组(转染anti-miR-142-5p)、anti-miR-con组(转染anti-miR-con)、miR-142-5p组(转染miR-142-5p mimics)、miR-con组(转染miR-con),NC组(未经任何处理的细胞);anti-miR-142-5p+si-con组(共转染anti-miR-142-5p与si-con)、anti-miR-142-5p+si-APLN组(anti-miR-142-5p与si-APLN).qRT-PCR与Western blot检测两组神经细胞中miR-142-5p与APLN的表达.ELISA法检测GSH与MDA含量.流式细胞仪检测细胞凋亡能力变化.双荧光素酶报告基因鉴定miR-142-5p的靶基因.Western blot检测Bax、Bcl-2蛋白表达.结果 miR-142-5p在癫痫细胞模型组神经细胞中表达上调(P<0.05).干扰miR-142-5p表达后大鼠神经细胞中GSH含量明显增加,MDA含量明显减少(P<0.05),细胞凋亡率显著降低(P<0.05),Bcl-2蛋白表达水平显著升高(P<0.05),Bax蛋白表达水平显著降低(P<0.05);双荧光素酶报告基因实验证明miR-142-5p可直接靶向结合APLN;沉默APLN的表达可部分逆转干扰miR-142-5p的表达对大鼠癫痫神经细胞的保护作用.结论 干扰miR-142-5p表达可能上调APLN的表达抑制神经细胞凋亡从而减轻癫痫发作后神经元损伤.

目的 探究注射用牛肺表面活性剂联合无创经鼻正压通气治疗新生儿呼吸窘迫综合征的临床疗效.方法 选取我院2017年4月～2018年4月收治的新生儿呼吸窘迫综合征患儿68例,依照治疗方案不同分为对照组和观察组各34例.对照组给予无创经鼻正压通气治疗,观察组给予注射用牛肺表面活性剂联合无创经鼻正压通气治疗.观察比较两组临床疗效,机械通气时间、氧疗时间、住院时间及血气指标改善情况.结果 观察组治疗总有效率为94.12％,高于对照组的70.59％,差异有统计学意义(P＜0.05);观察组机械通气时间、氧疗时间、住院时间均短于较对照组,差异有统计学意义(P＜0.05);治疗后观察组PaO2、血PH高于对照组,PaCO2低于对照组,差异均有统计学意义(P＜0.05).结论 注射用牛肺表面活性剂联合无创经鼻正压通气治疗新生儿呼吸窘迫综合征,能促进患儿康复,改善患儿血气指标,疗效显著.

Objective:To explore the phenotypic and genotypic characteristics of 3 Chinese families with dystrophinopathy, so as to provide the data for earlier diagnosis and therapy.Methods:The clinical, muscle pathology and electrophysiological data from the 3 families with dystrophinopathy were analyzed.The perpheral venous blood of 15 members from the 3 families was collected.Meanwhile, the known genes that were related to neuromyopathy were detected.Results:There were 8 patients in the 3 families.All the patients presented progressive weakness of extremities as the main manifestation, with elevated creatine kinase (CK) and myogenic changes in electrophysiological examination.The proband of family 1 was a 15 years old boy with 1 year history.He displayed limb weakness and accompanied with muscle pain after exercise.Muscle pathology only revealed denatured and atrophy muscle fibers, without necrosis and hyperplastic muscle fibers.The proband of family 2 was a 9 years old boy with 1 year history.His muscle pathology illustrated degeneration, necrosis, proliferation and lipid deposition muscle fibers.The proband of family 3 was a 16 years old boy with 10 years history.He exhibited generalized muscle atrophy, spine and chest deformity.His muscle pathology demonstrated classical muscular dystrophy changes.Gene detection gave information that deletion mutation in exons 45 to 47 of DMD gene in family 1 proband.c.2636 T> G mutation in exons 18 of DMD gene in family 2 proband, repeat mutation in exons 61 to 76 of DMD gene in family 3 proband; c.2636T>G was classified as pathogenic variation according to the guidelines for the interpretation of sequence variants of the American college of medical genetics and genomics guidelines. Conclusions:The phenotype of dystrophinopathy is related to genotype.A new mutation of DMD gene c. 2636T>G is discovered.Early patient with dystrophinopathy can only display pained weakness of muscle after exercise.Muscle pathology and gene detection should be performed as soon as possible.

Objective:To study the clinical, imaging and genetic characteristics of two Chinese families with oculopharyngeal muscular dystrophy (OPMD).Methods:The clinical data of the two families found in our hospital in August 2016 and May 2018 were analyzed. All the members were investigated in detail, and the clinical and imaging data of the probands were analyzed. Blood samples were collected from 22 members of the two families and PABPN1 gene analysis was performed. Results:There were 4 patients in family 1 with four generations and 4 patients in family 2 with three generations. The two probands presented ptosis, dysphagia at the age of 50 and 55. The proband of family 1 also showed diplopia, amyotrophy, weakness of proximal limbs, neurogenic changes in electromyogram (EMG), muscle fibers with rimmed vacuoles in muscle pathology, aspiration pneumonia in chest CT, and brainstem symmetric white matter lesions in cranial MR imaging. The proband of family 2 also showed eye muscle paralysis and lateral limb weakness, myogenic changes in EMG, bilateral parietal and right frontal lacunar infarctions in cranial MR imaging. Analysis of PABPN1 gene showed that the repeated mutation of PABPN1 trinucleotide (GCN) in 2 families was amplified from normal (GCG) 6(GCA) 3(GCG) to (GCG) 6(GCA) 3(GCG) 2(GCA) 3(GCG). Conclusion:OPMD has clinical heterogenicity; symmetrical white matter lesions in the brainstem might be found in cranial MR imaging; Chinese patients with OPMD have PABPN1 gene mutation, specificly manifested as (GCG) 6(GCA) 3(GCG) 2(GCA) 3(GCG) repeat mutations.

目的:分析抗菌药联合布地奈德治疗新生儿肺炎的疗效及对患儿并发症发生情况的影响.方法筛选2017年3月—2018年4月我院收治的新生儿肺炎患儿76例,采用随机数字表法分为观察组与对照组,各38例,对照组接受常规治疗,观察组采用布地奈德吸入(1 mL,bid)联合抗菌药治疗,比较两组疗效及并发症情况.结果治疗后,观察组总有效率高于对照组,差异有统计学意义(P<0.05);观察组气促、鼻阻、口唇紫绀、肺部啰音等病症消失时间均短于对照组,差异有统计学意义(P<0.05);观察组不良反应发生率低于对照组,差异有统计学意义(P<0.05).结论采用布地奈德雾化吸入+抗菌药治疗新生儿肺炎的疗效确切,可有效缓解患儿症状,且安全性高,值得临床推广.

目的:探讨围手术期并发Wemicke脑病伴严重低钠血症的发病机理、临床表现及诊断和防治措施.方法:回顾性分析围手术期并发Wemicke脑病的2例患者的临床资料,总结其临床表现、辅助检查、诊断、治疗和预后情况.结果:2例患者分别在术后第23、25日发生眼肌麻痹、共济失调、精神症状,且伴有严重低钠血症.结论:围手术期维生素B1补充不足易引起Wernicke脑病,伴发严重低钠血症.

目的:探讨丙戊酸钠治疗癫痫80例的临床效果观察.方法:将2014年5月～2015年5月在我院神经内科治疗的80例癫痫患者随机分为两组,对照组采用常规抗癫痫药物治疗,观察组采用丙戊酸钠治疗,比较两组治疗效果、症状控制时间、不良反应等.结果:观察组治疗有效率为90％,与对照组的72.5％相比疗效较高,差异显著(P<0.05);观察组临床症状恢复正常时间较对照组明显缩短,差异显著(P<0.05);观察组头晕乏力、恶心呕吐、皮疹、嗜睡、暴躁易怒等不良反应发生率明显低于对照组,差异有统计学意义(P<0.05).结论:丙戊酸钠治疗癫痫临床效果好,利于癫痫的快速控制,且安全性高,不良反应少,具有临床意义.

随着生活水平的提高,人们对蔬菜水果的保鲜营养的要求也越来越高.文章介绍了其共性:呼吸和保鲜的必要性及其规划,整合资源等有效利用.

Objective To investigate the clinical and molecular genetic features of idiopathic basal ganglia calcification ( IBGC) with acute cerebral infarction. Methods Clinical data of 1 patient with IBGC and his family were analyzed retrospectively. Results There were 9 patients in 3 generations family. The proband presented paralysis of left limbs, craniocerebral CT showed bilateral basal ganglia calcification and craniocerebral MRI discovered acute infarction of the right basal ganglia besides bilateral basal ganglia calcification. Until now, no obvious clinical symptom was found in the other patients except facial pigmentation. Bilateral basal ganglia calcification was found in all the patients. Karyotype analysis and electroencephalogram were normal. No mutation of SLC20A2 was found in the proband. Conclusions Besides typical the characters of craniocerebral imaging, IBGC can be accompanied with acute infarction and skin pigmentation. This family was not caused by SLC20A2 gene, which suggested genetic heterogeneity of IBGC.

目的：分析用依达拉奉治疗脑梗死的效果。方法选择2015年1月~12月1500例脑梗死患者,随机分为对照组和依达拉奉组,对照组采用阿司匹林联合低分子右旋糖酐治疗,依达拉奉组在对照组基础上加用依达拉奉治疗。结果治疗后7d和治疗后14 d,依达拉奉组神经功能缺损度评分(ESS)、日常生活能力评分(ADL)、焦虑自评量表评分(SAS)和抑郁自评量表评分(SDS)改善情况均显著优于对照组(<0.05)。结论在阿司匹林联合低分子右旋糖酐治疗基础上加用依达拉奉,能提高治疗脑梗死的临床疗效、能够提升患者生存质量、减轻患者焦虑和抑郁。

安阳地区藜麦品种对比试验结果表明,安藜3号、安藜4号在安阳市的种植表现最好,适宜在当地推广.

针对国内粮食库存积压,粮价下跌,农民增收乏力的农业生产问题,提出转变农业发展方式,发展特色农业;突出科学规划、龙头带动、品牌培育、科技支撑和三产融合;加强规模经营,提高农产品综合生产能力,加大农民增产增收的活力的几点发展措施.

藜麦属藜科双子叶植物,原产地主要分布于南美洲的玻利维亚、厄瓜多尔和秘鲁等地,是印加土著居民的主要传统食物,有5000多年的种植历史,由于其丰富、全面的营养价值,养育了印加人民,古代印加人称之为“粮食之母”,其营养价值受到了全世界的认可.近些年由于需求旺盛,我国也开始引种藜麦,安阳市农业科学院于2013年开始引种藜麦,通过2年的种植,摸索出了一些经验,同时也遇到了一些问题,现将遇到的这些问题和对策列举出来,以期能为种植藜麦的相关人士和行业的发展提供参考.

为了减少藜麦生长过高产生的倒伏问题,采用了不同浓度的矮壮素对58日龄的藜麦苗进行处理,结果显示浓度为3 200 mg/kg的矮壮素效果最好,浓度为3 200 mg/kg以上的矮壮素则会产生药害.

目的 观察枸橼酸西地那非鼻饲或口服和硫酸镁(MgSO4)静脉滴注治疗新生儿持续肺动脉高压(PPHN)的临床疗效.方法 选取2010年5月-2013年5月临床诊断为PPHN的38例患儿,两组患儿均常规保持气道通畅、保暖、机械通气等,其中22例应用西地那非1mg～2mg/kg·次,口服或者鼻饲;16例应用MgSO4,饱和量200mg/kg,20min～30min内静脉滴注,然后以20-50mg/kg·h维持静脉滴注.所有患儿分别于治疗前、后监测肺动脉压力(SPAP)、动脉血氧分压(PaO2)及体循环收缩压(SBP)的变换.结果 枸橼酸西地那非口服或鼻饲、MgSO4静脉滴注分别于治疗10 min～30min、2h、24h～48h后的SPAP、PaO2与治疗前相比有显著差异.但MgSO4静脉滴注组SBP明显下降,与治疗前相比有显著性差异;西地那非组SBP无明显差异.结论 枸橼酸西地那非和MgSO4均可有效治疗新生儿肺动脉高压,但西地那非起效更迅速,且具有高度肺血管选择性,对体循环收缩压没有影响.

OBJECTIVE To summary and analyze the stroke patients with hospital infections and explore the progn-sis of these patients cure and the changes of immunologic function ,so as to provide reference for preventing the infection after stroke in clinic and improve the quality of life of patients with stroke .METHODS A prospective study was conducted in our hospital from Jan .2012 to Jan 2013 on 636 cases stroke patients ,in which 55 cases with nosocomial infection as the infection group ,and the other 581 patients without nosocomial infection as the control group .Two groups were compared on prognosis and immune function .SPSS17 .0 software was used for statistical analysis .RESULTS The scores of NIH Stroke Scale(NIHSS)and Modified Rankin Scale(mRS) of the infection group were higher than the control group ,while the Barthel Index(BI) of the infection group was signifi-cantly lower than that of the control group (P<0 .05);Three weeks after treatment ,the NIHSS score and mRS score in both groups were decreased ,and the BI values were increased ,while the inprovement of mRS score and BI values of the infection group were better than the control group .The incidence rate of malnutrition on admission in infection group was 30 .91% ,which was higher than that of the control group ,which was 3 .27% .Three weeks after treatment ,the incidence ratr of malnutrition was 56 .36% in infection group ,which was still higher than that of the control group ,which was 4 .65% and the difference between the two groups was statistically significant (P<0 .05) .CONCLUSION The inhibition of immune function may increase the incidence of infection after stroke , which is not bentficial for the cure of patients ;should the imroved ,the prevention of infection should be steength-ened ,and the immune function for the patients should be improved .