目的:探讨磷酸肌醇信号通路在磷脂酰胆碱(PC)阳性伴放线放线杆菌(Aa)粘附和侵袭人脐静脉血管内皮细胞(HUVECs)过程中的作用.方法:采用磷酸肌醇信号通路中磷脂酶C(PLC)与G蛋白偶联受体结合过程的抑制剂U73122及其无活性的类似物U73343预处理HUVECs,分别通过粘附侵袭实验观察Aa对细胞粘附和侵袭能力;MTrr法检测Aa对不同预处理细胞损伤情况;Ca2+荧光探针检测Aa侵袭过程中细胞内Ca2+的动员情况.结果:与对照组相比,U73122预处理细胞后,PC阳性Aa的侵袭率降低至(12.62±2.10)％、细胞存活率升高、抑制了Ca2的增加(P＜0.05),粘附率则无显著差异(P＞0.05);U73343预处理细胞后,PC阳性Aa的侵袭率、粘附率、细胞存活率与非预处理组相比无统计学差异(P＞0.05);U73122拮抗了Aa引起的Ca2+向HUVECs内聚集(P＜0.05).结论:磷酸肌醇信号通路中G蛋白偶联受体与PLC的结合在PC阳性Aa的粘附、侵袭细胞和诱导细胞死亡的过程中发挥了重要作用.

Objective To explore the role of phosphorylcholine ( PC) during Aggregatibacter actinomycetemcomitans ( Aa) adhesion and invasion of human umbilical vein endothelial cells ( HUVEC) . Methods Non-attached plaque in periodontal pockets of 7 patients with aggressive periodontitis was collected and then inoculated on the selective medium TSBV plate. The phosphorylcholine positive expression Aa clinical strains were isolated through colony morphology, cata-lase test, Gram stain experiment, 16S rDNA and bacterial protein expression. Then, adhesion and invasion tests were taken to explore the role of phosphorylcholine during Aa pathogenisis process. Results After selective culture and spe-cific identification, one phosphorylcholine positive expression Aa clinical strain was found by Western blot. After pretrea-ted with anti-PC monoclonal antibody TEPC-15, the effect of Aa adhesion to and invasion of HUVEC was obviously de-clined ( 31. 87 ± 4. 22)% and ( 24. 63 ± 3. 55)% respectively. Conclusion PC helps PC positive Aa adhesion to and in-vasion of HUVEC.

Background: Acatalasemia is a rare genetic catalase deficiency that is inherited as an autosomal recessive trait. Although usually asymptomatic, a syndrome of oral ulcerations and gangrene may be present (Takahara's disease). In this report, we presented the diagnosis and 15-y periodontal treatments of an acatalasemia patient with Takahara's disease in China.Methods: To confirm the diagnosis of acatalasemia, intron 4 of the catalase gene was amplified and sequenced. Erythrocyte catalase activity was measured by ultraviolet spectrophotometer. Besides, periodontal treatments and 15 y follow-up were performed.Results: Direct sequencing showed a clear splicing mutation of guanine to adenine substitution at the fifth position of intron 4 in the patient. Erythrocyte catalase activity of the patient (5.2 MU/I, 4.6%) was 10% lower than the normal range (113.3 +/- 16.5 MU/I). After 15-y treatments, the periodontal pocket depth >= 4 mm and clinical attachment loss reduced to 30% and 3.7 +/- 1.2 mm.Conclusions: Based on these findings, a diagnosis of acatalasemia was established. And the periodontal therapies have achieved a stable periodontal status. (C) 2014 Elsevier B.V. All rights reserved.

Objective To discuss the association between uric acid (UA) and intracranial artery stenosis in patients with cerebrovascular disease. Methods Clinical data of 509 patients undergoing cerebral angiography were analyzed, and they were separated into four groups according to the findings of cerebral angiography:Intracranial artery stenosis group included 198 patients who had at least one intracranial large artery stenosis more than 50%; Extracranial artery stenosis group included 107 patients who had at least one extracranial large artery stenosis more than 50%; Cerebral artery stenosis group included 55 patients who had both intracranial and extracranial large artery stenosis more than 50%; and the control group included 149 patients who had none cerebral large artery stenosis. The relationship between UA level and intracranial artery stenosis was explored. Results The UA level of intracranial artery stenosis group was significantly higher than that of the control group (P<0.001), and the intracranial artery stenosis group had a marked higher rate of patients who had abnormal UA level (χ2 =7.388, P=0.007). The proportion of intracranial artery stenosis patients increased significantly with the increase of UA level (χ2 =9.230, P=0.026). Logistic regression analysis shows that UA level is one of the independent risk factors of intracranial artery stenosis (odds ratio[OR]=1.005, P=0.001). The relationship between extracranial artery stenosis and UA was negative. Conclusion The elevated UA level correlates with intracranial artery stenosis and it is an underlying independent risk factor of intracranial artery stenosis.

目的 在中国汉族人群中研究白细胞介素-6(IL-6)基因多态性、2型糖尿病和牙周炎三者之间的相关性.方法 通过病例对照模式,所有受试者被分成4组:单纯慢性牙周炎组(159例)、单纯2型糖尿病组(88例)、慢性牙周炎伴2型糖尿病组(110例)以及健康对照组(135例).采用多聚酶链反应-限制性片段长度多态性(PCR-RFLP)法测定IL-6-572单核苷酸多态性位点的基因型,并作质量检测.用2检验检验等位基因频率、基因型分布的差异.结果 在CC vs GC+GG模式的多重比较中发现,慢性牙周炎伴2型糖尿病组与健康对照组之间差异有统计学意义(P=0.006).等位基因频率多重比较,在慢性牙周炎伴2型糖尿病组与健康对照组之间差异有统计学意义(P=0.002).结论 IL-6-572位点的CC基因型和C等位基因可能是慢性牙周炎伴2型糖尿病的一个保护因素.

Objective To find out the chemical composition of the antigen reacting with TEPC-15 in Aggregatibacter actinomycetemcomitans(Aa),and the electrophoresis changes of the proteins and anti-TEPC-15 reactivity with and without proteinase K treatment were compared.Methods Bacteria cells were broken by Branson Sonifier,treated with proteinase K and separated on SDS-PAGE.The gels were first analyzed by Coomassie blue staining.Then the electrophoresis results were transferred onto membrane and immunoblot were done by using mouse anti-phosphorylcholine monoclonal antibody TEPC-15 as the primary antiserum and goat anti-mouse IgA as the secondary antiserum.Results After Coomassie blue staining,the sample without proteinase K treatment showed a variety of bands distributed from low to high molecular weight area.The samples treated with proteinase K revealed a single clear band of 29 kDa,which was not detected in the samples without treatment.This 29-kDa band could also be detected in a control sample with the same amount of proteinase K in PBS but no bacteria.Since the molecular size of proteinase K(Sigma) is 28.93 kDa,the result suggests that proteinase K was the source of the 29-kDa band.Immunoblot analysis showed a single band of 9 kDa with anti-TEPC-15 reactivity without proteinase K treatment and the proteinase K treatment eliminated the TEPC-15 reactivity with A.actinomycetemcomitans lysates.Conclusion The Aa antigen for TEPC-15 is a protein,since proteinase K treatment of the cell lysate abolished the reactivity.

Objective To detect the possible occurrence of the phosphorylcholine(ChoP) epitope in Aggregatibacter actinomycetemcomitans(Aa) serotype b strains.Methods Immunofluorescence microscopy,slot blot,and immunoblot were applied to detect expression of the ChoP in 12 Aa serotype b strains by using mouse anti-ChoP monoclonal antibody TEPC-15 as the primary antiserum and goat anti-mouse IgA as the secondary antiserum.Results Slot blot results revealed positive reactions in 5/12(41.6%) of strains tested.The ChoP could be detected by immunofluorescence microscopy implied that ChoP may be on the surface of Aa.Immunoblot results showed that ChoP on a structure of 9 kDa.The 5 positive strains could be detected by immunofluorescence,slot blot,and immunoblot simultaneously and 7 negative strains were all negative by all 3 methods.Conclusion Phosphorylcholine could be detected in Aa serotype b strains.And the epitope may be on a surface structure of 9 kDa.

心脏受累 牙周炎的主要致病因素是牙菌斑,口腔感染可引起急性或亚急性感染性心内膜炎. 牙周炎与急性心肌梗死和慢性冠心病的关系,近年来也得到印证.

广泛型侵袭性牙周炎(generalized aggressiveperiodontitis,GAgP)是一组发生于年轻人群,病变累及全口大多数牙齿,有严重而快速的牙周附着丧失和牙槽骨破坏的牙周疾病,发病率较低.同时伴有类风湿关节炎(rheumatoidarthritis,RA)的GAgP少有报道.笔者于1994年接诊一例伴BA的CAgP患者,经过15年的牙周系统治疗,疗效满意,现报告如下。

Objective To investigate the oral manifestation of acatalasia(AC),and clarify its diagnostic features and periodontal treatment protocol.Methods History of one patient suffering from AC was followed,and physical and oral examination were performed.Referred to the results of physical examination and erythrocyte catalase(catalase,CAT) activity,a diagnosis of AC was confirmed.Foundational periodontal treatment and 12 years' follow-up were performed.Results The oral lesions were manifested as prepubertal periodontitis.Erythrocyte CAT activity(52 mU/L,4.6%) confirmed the final diagnosis of acatalasia-periodontol destroy syndrome.Periodontal therapy has achieved good and stable results.Conclusion The early diagnosis can be made with the disease-specific clinical manifestations and CAT activity test.Early diagnosis and treatments of periodontal disease can receive a good prognosis.

Objective To determine serotypes of 29 Aggregatibacter actinomycetemcomitans strains from blood isolates by polymerase chain reaction. Methods ATCC 29523 (serotype a), ATCC 43718 (serotype b), ATCC 33384 (serotype c), IDH 781 (serotype d), IDH 1705 (serotype e) and CU 1000 (serotype f) were used as reference strains. Six pairs of oligonucleotide primers specific for gene clusters involved in the biosynthesis of serotype specific polysaccharide antigens were designed. The specificity of the primers were evaluated by the reference strains of Aggregatibacter actinomycetemcomitans. Serotypes of 29 Aggregatibacter actinomycetemcomitans strains from blood iaolates were determined by PCR with the primers. Results Each pair of primers can specifically identify one serotype of Aggregatibacter actinomycetemcomitans. No cross reaction was observed in all strains. The PCR product sizes were: 428 bp (serotype a), 298 bp (serotype b), 559 bp (serotype c), 690 bp (serotype d), 211 bp (serotype e) and 232 bp (serotype f). Of the 29 strains from blood isolates, 16 strains were serotype b (55%), 4 strains were serotype a (14%), 4 strains were serotype c (14%), 2 strains were serotype d (7%), 2 strains were serotype f (7%). One strain could not be serotyped by PCR assay. Conclusion Serotype b was the most frequently detected serotype of the Aggregatibacter actinomycetemcomitans strains from blood isolates. Serotype a and c could be detected more often than the other serotypes.

OBJECTIVE:To investigate the mutational characteristics of the cathepsin C gene (CTSC, also known as dipeptidyl-peptidase I gene, DPP I) in a family of Han nationality with Papillon-Lefevre syndrome, and to provide the molecular basis for the phenotype.METHODS:Genomic DNAs were extracted from the proband, his parents and younger sister after informed consent. Polymerase chain reaction and direct DNA sequencing were carried out to screen the mutations of the cathepsin C gene.RESULTS:Compound heterozygous mutations of the cathepsin C gene were identified in the patient. The patient carried one frameshift mutation 116delG in exon 1, one heterozygous mutation C255S in exon 6, one missense mutation F314S and one sense mutation E335E in exon 7. The four changes were novel mutations of the cathepsin C gene, which had not been reported previously. None of the mutations were detected in normal controls.CONCLUSION:Mutations of the cathepsin C gene are probably responsible for the phenotype of Papillon-Lefevre syndrome in this family.

目的探讨烤瓷夹板松牙固定术的临床护理工作重点。方法选择行烤瓷夹板松牙固定术的牙周炎患者60例,对基牙预备、数次印模制取、临时冠制作、夹板粘固等的护理配合工作进行分析和总结。结果烤瓷夹板密合、稳固,患者咀嚼功能、生活质量明显改善。结论规范、熟练的护理配合是治疗成功的重要保证。

Objective The current study was aimed to evaluate the clinical effect of periodontal splints made of porcelain-fused-to-precious-alloys in advanced periodontitis. Methods Sixty patients with advanced chronic or aggressive periodontitis were chosen. After routine scaling and root planning, loosen teeth and missing teeth were restored by splints made of porcelain-fused-to-precious-alloys. The clinical effects of the splints were evaluated according to the changes of probing depth, gingival recession, clinical attachment loss and alveolar bone density, and oral health impact profile was filled in by all the patients to reflect their own evaluation of the splints. Results After the treatments, all the clinical indicators, especially probing depth and clinical attachment loss, were improved significantly. The masticatory efficiency was obviously improved while the patients were satisfied at the splints, and the psychological situations were tremendously improved. Conclusion The clinical application of the splint made of porcelain-fused-to-precious-alloys is very satisfactory to patients, and improved the life quality. Therefore, the splint has good prospects in advanced periodontitis.

掌跖角化-牙周破坏综合征(papillon-lefevre syndrome,PLS)是一种临床上罕见的疾病,患病率约为 1～4百万分之一.PLS以掌跖过度角化、乳牙和恒牙牙周组织快速严重破坏为特点,现有资料表明:PLS患者的预后差,乳牙早失,恒牙在13～15岁可全部脱落.

Objective To investigate the relationship between FcγRⅢB gene polymorphisms and susceptibility to chronic periodontitis in Chinese patients. Methods DNA samples with buccal swabs were collected from 63 severe CP patients, 103 initial to moderate CP patients, and 80 healthy controls in Chinese Han nationality. The FcγRⅢB gene polymorphisms were analyzed with PASA. Results There were no significant genotype distribution differences of FcγRⅢB between any two of the three subject groups. Conclusion Further study was needed since we did no find the relationship between the FcγRⅢB gene polymorphism and periodontitis in this present study.

Objective:To explore the clinical treatment effects of scaling and/or local application of Perio on dental implantitis.Methods:15patients with2or more dental implants individually suffering from dental implantitis were chosen and randomlyassigned to receive scaling,local application ofperio,orcombined treatment.Parame-ters including plaque index(PLI),sulcular bleeding index(SBI)and probing depth(PD)of pockets were mea-sured at baseline,4and12weeks after treatment.Results:Three treatments significantly decreased PLI,SBI and PD,however the effects of the combined treatment was lasting to12weeks.Conclusions:The scaling and local application of Perio were effective for dental implantitis,the combined treatmentwas more efficientand last-ing.

PURPOSE:The purpose of this study was to investigate the relationship between TNFalpha-308 gene polymorphisms and susceptibility to chronic periodontitis in Chinese patients.METHODS:DNA samples with buccal swabs were collected from 63 severe CP patients, 103 initial to moderate CP patients, and 80 healthy controls in Chinese Han nationality. The TNFA-308 gene polymorphisms were analyzed with PCR-RFLP. The data were analyzed by X(2) test using SPSS10.0.RESULTS:There were no significant genotype distribution differences of TNFA-308 between either two of the three subject groups.CONCLUSION:Further study was needed since we did not find the relationship between TNFA-308 gene polymorphism and periodontitis in this present study.

目的观察克林霉素治疗牙周炎的临床效果.方法 74例慢性牙周炎患者随机分入克林霉素治疗组和甲硝唑治疗组,治疗前和用药1周后检查牙龈指数、龈沟出血指数、探诊深度及药物不良反应.结果两组病例治疗 1周后各临床指标较治疗前显著降低,1周后克林霉素治疗组牙龈指数、龈沟出血指数比甲硝唑治疗组低,两组探诊深度降低无差别,克林霉素治疗组用药后不良反应较甲硝唑治疗组少且轻微.结论克林霉素能明显改善牙周炎的临床症状,治疗牙周炎效果显著.

目的探讨酸性根面处理剂枸橼酸(pH 1)、盐酸四环素(100 μg/ml,pH 2)与非酸性根面处理剂24%EDTA(pH 7)对根面的影响.方法健康牙根和牙周炎患牙根经洁治和根面平整后,分别用枸橼酸、盐酸四环素及EDTA处理根面牙骨质和牙本质,扫描电镜观察.结果酸性和非酸性根面处理剂均能去除根面玷污层、表层钙化层,不同程度地显露根面胶原纤维,但酸使纤维结构受到不同程度地破坏,EDTA对根面纤维结构无影响.结论 EDTA较大程度地保留根面纤维结构,作为根面处理剂有可能优于酸性处理剂.