ABSTRACT This study investigates the regulatory effects of low‐voltage electrostatic field (LVEF) in synergy with melatonin (MT) on the postharvest quality of sweet cherries pretreated with cold atmospheric plasma (CAP). “Rainier” sweet cherries ( Prunus avium L.) served as the experimental subjects. After uniform CAP pretreatment, the fruits were subjected to MT immersion (100 μmol L −1 ), LVEF treatment (200 V), or a synergistic combination of both, and subsequently stored at 4°C for 20 days. Throughout the storage period, we periodically monitored color, texture, browning, weight loss, decay, and associated physiological indices. The results demonstrate that the synergistic treatment of LVEF and MT yields superior preservation efficacy. By Day 20, this group maintained L * and a * values of 48.59 and 13.22, respectively, with a total color difference (Δ E *) of merely 5.14, exhibiting significantly higher color stability than other treatments ( p < 0.05). Furthermore, the LVEF+MT group showed a firmness decline of only 19.07% (988.40 g) and a skin penetration force loss of 5.36%, both significantly lower than the control (CK) group's 43.63% and 29.10%, respectively. Stem browning rates and fruit browning indices were recorded at 19.67% and 3.65%, representing reductions of 80.32% and 78.32% compared to the CK group. Additionally, weight loss (4.42%) and decay rates (6.43%) decreased by 59.73% and 65.76% relative to the CK group. Total soluble solids (TSS) were maintained at a high level (17.97% on Day 20), and pH fluctuations were minimized (final value 3.77). Principal component analysis (PCA) further confirms that the LVEF+MT group achieved the highest composite scores across all storage intervals. In summary, the synergistic application of LVEF and MT, following CAP pretreatment, effectively retards postharvest quality deterioration in sweet cherries, offering a novel strategy for the preservation of perishable fruits.

Because of its unique flower shape, brilliant color, long flowering period, and high commercial value, Anthurium andraeanum Linden is one of the leading potted flowers. Polyploid germplasm resources have important utilization value in the study of genetic evolution of species and the breeding of new varieties. The A. andraeanum industry also needs newer polyploid varieties, which requires more polyploid parental materials. This study was envisaged to obtain high-quality new tetraploid germplasm of A. andraeanum also known as ‘Pink Champion.’ Specifically, the callus of A. andraeanum was treated with 0.5, 1.0, 2.0, and 4.0 g L−1 of colchicine for 2, 4, 6, and 8 h, respectively, and was then transferred to the culture medium of MS medium + 6-benzylaminopurine 2.0 mg L−1 + naphthylacetic acid 0.5 mg L−1. After 75 d being kept in the same culture vessel, the survival rate of callus and the number of regenerated plants were counted, and the regenerated plants were identified as tetraploid using morphological detection, microscopy, chromosome counting, and flow cytometry. The results showed that the concentration of colchicine was inversely proportional to the survival rate of callus and the number of induced regenerated plants. The highest tetraploid induction rate of 9.34

Effects of cinnamic acid (CA) on growth and endogenous hormone content in the roots of cucumber and figleaf gourd seedlings were studied and explained the reason why figleaf gourd was better in resisting CA stress than cucumber using their seedlings as experimental materials. The effect of CA at different concentrations (0, 0.05, 0.10, 0.15, 0.20 and 0.25 mmol/l) was analysed on primary root elongation, total root elongation, average diameter of total roots, total root volume, and total root tips as well as the effects of 0 and 0.25 mmol/l CA on root growth, root density, root hair growth rate, indole acetic acid (IAA) content and ethylene (ET) production rate. The results showed that CA stress decreased the primary root elongation, total root elongation, total root tips, Grade III root and root hair growth rates of both plants. The inhibiting effect of CA was stronger on cucumber than figleaf gourd. Besides, CA stress increased their average diameters of total roots and root hair densities. The increase in the average diameter of total roots of cucumber was larger than that of figleaf gourd while its increase in root hair density was far lower. Apart from that, 0.25 mmol/l CA stress increased the IAA contents and ET production rates in both kinds of roots. The increase in IAA content in cucumber root was 16% higher than in figleaf gourd. However, the increase in the ET production rate of cucumber root was 51% lower than figleaf gourd root. In conclusion, higher concentration IAA could inhibit root growth. Under CA stress, the increase in the IAA content in figleaf gourd root was lower than that in cucumber root, indicating that CA exerted a weaker inhibiting influence on figleaf gourd. Besides, higher ET production rate contributed to the increase in root hair density. Under CA stress, figleaf gourd root had a higher ET production rate than cucumber root, thus having more root hairs.

Under the background of new agricultural science,training skilled, innovative and compound talents is an important demand for the rapid development of garden industry in China. Taking the major of landscape architecture and landscape architecture in Suqian University as an example, this paper puts forward the measures for the cultivation system of practical and innovative skills of landscape floriculture. According to the flowers seedling stage,marketing and after-sales as the time axis of professional curriculum experiment; This paper will create platforms for entrepreneurship and innovation and set up experiments on innovative courses. This paper also use school enterprise cooperation which students go to the enterprises to carry out the part of the curriculum experiment. Reforming the examination methods of experimental courses which innovative credits were brought into the course score was created. Reform the examination method of experimental courses and carry out the system of incorporating innovative credits into the course scores. It also puts forward the existing problems and improvement measures in constructing the course practice system, these measures will provide successful experience for the curriculum building and training system of the great practical innovation skills of agriculture specialty under the background of new agriculture.

为解决"植物生理学"侧重于课程知识传授与实验能力培养,忽视对学生进行德育教育的现状,本文以植物生理学课程中的两个知识点——"合理灌溉"与"植物激素"为例,分析了课程教学内容与课程思政特点,挖掘课程中蕴含的重要思政元素;探索了将这些思政元素引入课堂的适当方式;并调研了课程思政的效果——学生的家国情怀、团队协作能力、生态环保意识和创新精神的掌握能力.提出"植物生理学"将知识传授、能力培养和价值塑造三者有机融合的方法,以期为"植物生理学"全部课程内容和相关课程的课程思政建设提供参考.

多肉花卉以其奇特外形和经营价格备受青睐,但多肉花卉存在育种周期较长、雌雄异熟、种子量小等问题,难以适应农业产业化需求.本文探讨了多肉植物快繁体系技术,分析了多肉植物组织培养技术存在的问题,并提出了前景展望,为多肉植物的培养、应用提供参考.

针对高校园林专业实践教学中存在的问题,根据园林绿化产业链(园林规划设计、园林工程施工和园林植物种植养护)的特点,宿迁学院园林专业利用"花木之乡"企业较多的优势,与本地企业深度合作,并通过实践教学体系构建、项目教学法串联实践教学环节、校企合作实践教学课程体系、行业资源库建设、实践教学平台集约化管理、教学团队内外交流机制以及人才评价体系优化等措施,开展基于产业需求的实践教学改革,构建一套适应地方应用型人才的项目化实践教学体系,提升了实践教学质量,为园林专业创新性应用型人才的培养奠定了坚实的基础.

为了探究根系边缘细胞(root border cells,RBCs)在植物抗盐胁迫中的作用和机制,以白籽南瓜种子为试材,研究0、50、100、150、200和250 mmol/L NaCl对植株根长、RBCs数目、RBCs活率和RBCs黏胶层厚度的影响.结果表明,NaCl胁迫降低了植株根系的长度,随着NaCl浓度的增加,根系生长受到的抑制更明显;随着NaCl胁迫时间和浓度的增加,根系RBCs数目和活率也呈现不同程度的降低,其中200 mmol/L NaCl胁迫36 h的抑制效果最明显;但RBCs黏胶层厚度随NaCl胁迫浓度的增加而增加,200 mmol/L NaCl胁迫的RBCs黏胶层厚度最大.总之,NaCl胁迫对白籽南瓜根系的生长有一定的伤害作用,RBCs通过自身死亡和增加黏胶层厚度来减轻这种伤害.

校企合作共建园林花卉学课程,培养基础知识扎实、专业技能突出、创新能力较强的应用型人才,是我国花卉产业快速发展的重大需求.该文以宿迁学院园林和风景园林专业为例,提出了校企合作共建园林花卉学的主要改革措施,主要包括:按照花卉产业链的逻辑轴线构建课程内容;按照花卉产业链的逻辑顺序凝练专业技能,包括花卉的育苗技能、花卉的栽培和管理技能、花卉主要病虫草害和逆境的防控技能、花卉的采后处理技能和花坛的设计和规划技能;培养学生的创新创业能力.

壁画在历史舞台上扮演着重要的艺术角色,近年来,随着人们审美观念的变化,单一材料的壁画创作已经不能达到人们的要求,故壁画创作正积极融合优秀的艺术创作手法及形式而向多元化发展.干花艺术具有造型自然、观赏价值高、耐存放等特点,深受人们的喜欢.干花壁画既具备了长久使用的性价比,也具备承载艺术信仰及精神寄托的能力,给人带来贴近自然又富有创意的艺术审美.干花壁画创作可在一定程度上打破壁画发展的瓶颈,也可进一步促进干花产业发展.本文从花材的选择、花材的干燥处理、保存及标本壁画创作等方面总结了制作干花壁画的整个工艺流程,并以宿迁学院校徽为实例,对干花壁画作品的分析、设计与制作过程进行详细说明,为植物标本壁画创作提供一些理论和实践参考.

以多肉植物'特玉莲'叶片为试材,采用鳞片扦插的方法,研究了扦插基质的种类、配比和生长调节剂的种类、浓度对芽诱导及植株缀化的影响,以期探索'特玉莲'芽诱导和缀化的最佳条件,为多肉植物规模化繁育高质量的幼苗提供参考依据.结果表明:蛭石为诱导芽和新头的最佳培养基,扦插90 d时每个叶片诱导的芽和新头分别为2.4个和2.0个,其成活率高达100％;KT和2,4-D对芽、新头和叶片的诱导没有促进作用,NAA、6-BA、IBA、IAA和GA3均能促进三者的诱导,其中20 mg·L-1 NAA浸泡20 min为最佳处理方式,每片叶诱导的芽、新头和叶片分别达到4.8个、3.2个和36.8片;诱导缀化最佳的生长调节剂组合为80 mg·L-1 NAA+800 mg·L-1 6-BA等体积混合溶液.

随着我国广大城镇与乡村环境中绿地设计与建造水平的不断提升,对风景园林从业者专业水平的要求也在不断提高.基于现阶段高校风景园林专业绘画表现类课程教学发展现状,提出具体的教学改革方案,以提高风景园林专业教学效果和质量.

A highly efficient regeneration system of Anthurium andraeanum Linden was established using root segments as explants of four genotypes, Arizona, SWD, OYH, and Mississippi. The root explants were firstly incubated on callus induction media containing NH4NO3 level of 206 mg L−1 or 825 mg L−1, and the calluses were formed from both ends of the root segments after 1 mo of culture. Root-derived calluses were then transferred to shoot regeneration media with NH4NO3 level of 412 mg L−1 or 1650 mg L−1, and numbers of shoot were successfully produced from the surface of calluses after 2 mo of culture. Medium containing 206 mg L−1 NH4NO3 induced more callus compared to 825 mg L−1. In contrast, the number of shoots per callus was the same between the above two shoot regeneration media indicating that NH4NO3 level had little effect on shoot regeneration. Long-termin vitro maintenance of the callus-bearing shoot initials was possible without compromising shoot regeneration and subsequent rooting. Histological analysis showed that early cell division was initiated from the inner cortical parenchyma cells near the vascular tissue and from the vascular parenchyma cells adjacent to the xylem vessel. The primary callus mainly comprised inner parenchyma cells with scattered procambial-like cells and xylem elements and two different meristematic zones beneath epidermal layers. Transfer of callus to the regenerative medium caused meristemoid formation and subsequent shoot primordia production. Calcium oxalate crystals and tannin-like deposits were visible on the outer callus layer or within the cotyledon-like structure of shoot primordium throughout callus development. Starch grains were abundant in root explants, but disappeared in the profusely growing primary callus, and were mobilized in the shoot primordium, indicating energy requirements of these two developmental stages. Flow cytometry analysis demonstrated that ploidy levels were maintained during in vitro cultures, including long-term regenerated plants. The inter-simple sequence repeat analysis indicated genomic DNA stability of Arizona and OYH but occasional genetic variation in primary regenerates of SWD and Mississippi.

以唐山师范学院园艺专业为例,阐述园艺专业培养方案科学的构建.首先要依据市场、企业、科研院所和职业院校对人才培养的需要,提炼出园艺专业人才必须具备的专业技能;其次依托专业技能,构建园艺专业核心课程体系;然后依托园艺专业核心课程体系构建实践教学体系;进而依托实践教学体系构建实验实践教学平台和教学手段;最后形成完善的专业培养方案.

为了建设应用技术类型高等学校的应用型专业,探索了园艺专业实践教学体系的构成要素,主要包括应用技术类型高校园艺专业培养目标和能力的定位,园艺专业核心技能的凝练,实验、实践课程体系的设置,园艺专业核心技能的强化训练,园艺专业见习、毕业实习及专业教师队伍的建设.该实践教学体系为培养具有一定实践经验和专业核心技能的园艺专业人才提供了保障.

以红掌实生苗根段为外植体,通过在培养基中添加抗生素来抑制病原菌的快速繁殖,并建立了完整的再生体系.结果表明:硫酸链霉素抑制病原菌的效果最好,其次是卡那霉素,庆大霉素抑菌效果最差;30mg·L-1的硫酸链霉素因抑菌效果好,保持更高的根段活率而更适合在组织培养中应用;愈伤组织诱导的最佳培养基为MS+6-BA1.0mg·L-1+2,4-D0.5mg·L-1+硫酸链霉素30mg·L-1,愈伤组织增殖的最佳培养基为MS+6-BA1.0mg·L-1+2,4-D0.8mg·L-1,丛生芽诱导最佳培养基为1/2MS+6-BA0.5mg·L-1,丛生芽生根最佳培养基为1/2MS+NAA0.2mg·L-1,生根后的幼苗,移栽成活率高达100％.

基于社会对应用型大学园艺专业实践技能人才的迫切需求,从职业院校人才培养模式中汲取营养,探讨园艺专业核心技能的范畴.提出园艺专业人才必须掌握的十项核心技能:园艺植物的育苗技能、植物组织培养技能、园艺植物栽培和管理技能、园艺植物病虫草害的防治技能、日光温室和大棚的管理技能、日光温室的设计技能、产品采后保鲜与营销技能、花艺设计和盆景制作技能、园林设计与规划技能以及口头表达和科技论文写作技能,为培养具有一定实践经验和技能的园艺专业人才提供了保障.

In the present study, effects of cinnamic acid (CA) concentrations on root border cells (RBCs) of the seedlings of cucumber and pumpkin were investigated. Two different concentrations of CA along with a control i.e., 0, 0.125 and 0.250 mmol L-1 were used. Their effects on numbers, survival rates, mucigel thicknesses, apoptosis rates of RBCs and the root activities were monitored. Survival rates of RBCs and root activities of both cucumber and pumpkin were decreased at 0.125 and 0.250 mmol L-1 CA. These inhibitory effects were more significant on cucumber. However, the mucigel thicknesses and apoptosis rates in RBCs of both cucumber and pumpkin were increased at 0.125 and 0.250 mmol L-1 concentrations of CA. In comparison with cucumber, greater mucigel thickness and lower apoptosis rates of RBCs were detected in pumpkin. At a concentration of 0.250 mmol L-1 CA, while RBCs attached to the root tips, root activities decreased by 18.8% in cucumber. This change was insignificant in pumpkin. After removing the RBCs, the root activities of both cucumber and pumpkin were sharply inhibited by 0.250 mmol L-1 CA. This inhibition was higher in cucumber than pumpkin. It has indicated that, RBCs could increase plant's resistance to CA stress, which was characterized by low apoptosis rate and thick mucigel. The resistance of RBCs in pumpkin to CA stress was stronger than RBCs of cucumber. (C) 2017 Friends Science Publishers