Shipi powder (SPP) is a classical traditional Chinese medicine prescription used to warm yang, strengthen the spleen, promote qi movement, and eliminate retained fluid. It is widely used in the clinic for the treatment of edema caused by various conditions. However, the comprehensive and simultaneous characterization of the major constituents of SPP in vitro and in vivo has remained unclear, which has limited the identification of its active substances and its further clinical application. Therefore, in this study, a chemical composition database of SPP was established. An ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UHPLC-Q-TOF-MS) method was developed to characterize the constituents of SPP in vitro and in vivo. A total of 217 compounds were identified in vitro, including 57 alkaloids, 8 phenylpropanoid glycosides, 14 lignans, 41 flavonoids, 41 terpenoids, 15 gingerols, 7 lactones, 16 organic acids, and 18 other compounds. Meanwhile, 151 components were detected in vivo, including 93 prototype components and 58 metabolites, in rat plasma, bile, urine, and feces. In summary, this study systematically characterized the components of SPP in vitro and in vivo for the first time. These results provide a useful basis for identifying the pharmacodynamic substances of SPP and for guiding its clinical application.

Background Semen Sojae Praeparatum (SSP) exhibits both preventive and therapeutic effects against drug-induced liver injury (DILI). Traditionally, SSP is used in combination with Gardeniae fructus to prevent its hepatotoxicity. Isoflavones, the primary components of SSP, can mitigate DILI induced by chemotherapeutic agents such as acetaminophen and cisplatin. However, the potential of SSP to alleviate the hepatotoxicity of triptolide (TP, a prototypical compound in DILI research) remains unexplored. Purpose This study aimed to explore the protective effects and potential mechanisms of SSP on TP-induced liver injury. Methods The phytochemical profile of the SSP extracts was characterized using UPLC-Q-TOF-MS. Hepatoprotective effects of SSP were assessed using a TP-induced liver injury mouse model. The mechanisms were predicted by metabolomic and proteomic analyses, and further elucidated by RT-qPCR, western blotting and transmission electron microscopy. Results Isoflavones were identified as the main components of the SSP extracts. SSP treatment alleviated TP-induced abnormalities in serum biochemical markers, liver index and pathological damage. Multi-omics analysis revealed SSP modulated bile acid (BA) metabolism and autophagy, with Keap1 serving as a core protein. Furthermore, SSP reduced intrahepatic BA accumulation by enhancing hepatic BA transport rather than inhibiting BA synthesis. Additionally, SSP reversed TP-induced abnormalities in Keap1 and p62 expression and nuclear translon cation of the Nrf2 transcription factor, and mitigated oxidative imbalance and autophagic cell death. Conclusions SSP ameliorated TP-induced liver injury by modulating bile acid homeostasis and the Keap1/Nrf2/p62 pathway, thereby alleviating oxidative stress and excessive autophagy.

Objective: To investigate the differences in odor characteristics and volatile components between raw and wine‐processed Chuanxiong Rhizoma and to establish a rapid differentiation method based on taste, smell, color, and volatile chemical components. Methods: Electronic tongue, electronic nose, and ultraviolet spectrophotometer were used to analyze 23 batches of raw and wine‐processed Chuanxiong Rhizoma. Principal component analysis, one‐way ANOVA, and orthogonal partial least‐squares discriminant analysis were employed to differentiate between Chuanxiong Rhizoma and alcoholic Chuanxiong Rhizoma. Headspace gas chromatography–mass spectrometry (HS‐GC–MS) and multivariate statistical analysis were used to identify potential differential components between raw and wine‐processed Chuanxiong Rhizoma. Correlation analysis of these differential components with odor and color was performed to identify the main compounds responsible for the differences in odor and color. Results: The electronic tongue analysis showed that after alcohol processing, Chuanxiong Rhizoma exhibited a significant decrease in aftertaste and umami, a notable reduction in saltiness, and a significant increase in sweetness, enabling rapid differentiation between raw and wine‐processed Chuanxiong Rhizoma. The electronic nose analysis indicated that odor differences were primarily detected by sensors W1W and W2W, which can be used to quickly distinguish between raw and wine‐processed Chuanxiong Rhizoma. The ultraviolet spectrophotometer results showed no significant color differences between raw and wine‐processed Chuanxiong Rhizoma, making it difficult to distinguish between the two. HS‐GC–MS identified 18 differential components between raw and wine‐processed Chuanxiong Rhizoma. Pearson correlation analysis of volatile components and odor revealed that the main differential compounds include monoterpenes, alcohols, and aliphatic aldehydes. Notably, isoterpinolene, isoamyl alcohol, 2‐ethyl‐2‐hexenal, and hexenal were found to significantly contribute to odor differences, indicating a relationship between these differential components and the efficacy changes in Chuanxiong Rhizoma before and after wine processing. Conclusion: The combined use of the electronic tongue, electronic nose, and HS‐GC–MS enables rapid and accurate differentiation between raw and wine‐processed Chuanxiong Rhizoma. This method is valuable for the processing and quality control of traditional Chinese medicines.

This study employed an integrated approach that combined ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS) with network pharmacology to systematically characterize the phytochemical components and metabolites of Danggui Jianzhong decoction (DGJZD), as well as to elucidate its underlying mechanism. The results showed that a total of 109 compounds were identified. A total of 184 DGJZD-related xenobiotics were screened out in rat bio-samples. Network pharmacology identified 32 active components connecting 25 targets, and the data from the network pharmacology analysis suggested that inflammatory and cancer signaling pathways, EGFR tyrosine kinase inhibitor resistance, PI3K-Akt signaling pathway, and VEGF signaling pathway contribute significantly to the therapeutic effects of DGJZD on gastric ulcer (GU). PTGS2, IL2, PTGS1, PPARG, and EGFR have a high degree and have been proved to have a strong impact on the pathological process. We validated the stable and tight binding of PTGS2, IL2, PTGS1, PPARG, and EGFR with the related core components through molecular docking and molecular dynamics simulation. Taken together, the study clearly gave a comprehensive metabolic profile of DGJZD in vivo first. Our work reveals to study the material basis of the protective effects of DGJZD against GU and the underlying mechanisms.

To study the prototype components and metabolites at different time points in the serum of rats after oral administration with Shaoyao Gancao Decoction (SGD), and to analyze their metabolism regularities in vivo. The UPLC-Q-TOF-MS/MS method was used to analyze the drug-containing serum samples at different time points. The mass spectrometry data were preliminarily processed using Analyst® TF 1.7.1 Software, followed by advanced analysis and visualization by PeakView 2.0 and Masterview1.0 to determine the retention time and relevant details of prototype components and metabolites. The MetabolitePilot™1.5 was employed to analyze the prototype components and metabolites in drug-containing serum samples at different time points, systematically summarizing their in vivo metabolic profiles. A total of 79 prototype components and 527 metabolites were detected in serum samples collected at 10 different time points, including flavonoids, terpenoids, volatile oils, organic acids, alkaloids, coumarins, etc. Of these, 11 prototype components were detected at all time points in the positive ion mode, and 19 were detected in the negative ion mode. Specifically, seven key components, including glycyrrhetinic acid, isoglycyrrhetinic acid, macedonic acid, diisobutyl phthalate, dibutyl phthalate, vitexin, and linoleic acid, were detected in both ion modes. This article is the first to analyze the metabolites of drug-containing serum at 10 different time points. The predominant metabolic processes in vivo included oxidation, reduction, hydrolysis, glucuronidation, sulfation, acetylation, methylation, and amino acid conjugation. The study revealed detailed data about the effective substances in SGD having a potential protective role against liver injury, which will support further investigation of the effective ingredient group of SGD and associated mechanism(s).

This study aims to investigate the correlations of the appearance traits, total antioxidant capacity, and component content of Forsythiae Fructus processed by different methods, explore the effects of different processing methods on the abovementioned three aspects of Forsythiae Fructus, and screen out the internal and external indicators that have important effects on its quality. It determined the length, diameter, stem length, chroma value L~*, a~*, b~*, and other appearance indexes and antioxidant activity of Forsythiae Fructus processed by different methods. The content of forsythiaside A, rutin, forsythin, pinoresinol, and phillygenin was determined by ultra performance liquid chromatography(UPLC). Correlation analysis, principal component analysis(PCA), orthogonal partial least squares discriminant analysis(OPLS-DA), and independent sample t-test analysis were performed on the appearance indexes and the component content. The correlation analysis showed that there were differences in the appearance traits and the component content. L~* and E~* had highly significant negative correlations with pinoresinol and phillygenin(P<0.01) and significant positive correlations with forsythiaside A(P<0.05). There were a highly significant negative correlation between a~* and forsythiaside A(P<0.01) and highly significant positive correlations of a~* with pinoresinol and phillygenin(P<0.01). There were a highly significant positive correlation between b~* and forsythiaside A(P<0.01) and highly significant negative correlations of b~* with pinoresinol and phillygenin(P<0.01). The total antioxidant capacity had highly significant negative correlations with pinoresinol and phillygenin(P<0.01). The PCA results showed that there were differences among Forsythiae Fructus samples processed by different methods. OPLS-DA marked five important indicators, which were forsythiaside A, stem length, E~*, L~*, and b~*. The results of independent sample t-test showed that the content of forsythiaside A, pinoresinol, and phillygenin, the total antioxidant capacity, and the appearance traits such as L~*, a~*, b~*, and E~* were significantly different between the Forsythiae Fructus samples processed by steaming and boiling(P<0.05). According to content determination and a related biological activity analysis, steaming is a good choice from the perspective of improving the stability of chemical constituents and antioxidant activity of Forsythiae Fructus. From the point of view of improving the stability of chemical constituents and anti-inflammatory and anti-cancer activities of Forsythiae Fructus, it is recommended to use boiling as the processing method. Based on the above analysis methods, the main indexes for the appearance traits of Forsythiae Fructus processed by different methods are powder chroma value(L~*, a~*, b~*, E~*), stem length, and total antioxidant capacity, and those for chemical constituents are the content of forsythiaside A, pinoresinol, and phillygenin. This study provides reference for seeking scientific processing methods of Forsythiae Fructus.

Sanhua Decoction (SHD) is a classic prescription for the treatment of stroke in the clinic. Based on the combination strategy in vitro and in vivo, the chemical constituents of SHD were characterized by ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry, and the metabolites of five effective anthraquinone aglycones (emodin, chrysophanol, rhein, aloe-emodin, and physcion) of rhubarb in SHD were studied. A total of 222 compounds were isolated and identified in vitro, including 50 flavonoids and their glycosides, 41 coumarins, 22 anthraquinones, 22 tannins, 14 phenylpropanoids, 16 alkaloids, 18 organic acids and their esters, 14 lignans, six anthrones, and 19 other compounds. A total of 111 prototype components were isolated and identified in vivo. Among them, 26, 82, 101, and 46 prototype components and 87 metabolites were detected in plasma, urine, feces, and bile for the first time. This study provides a basis for the identification of chemical components in vivo and in vitro and the analysis of potential pharmacodynamic components of SHD, and provides a basis for further study of pharmacodynamic mechanism.

The enhanced efficacy of vinegar-processed Cyperus rotundus (VCR) in treating primary dysmenorrhea (PD) has been observed. However, the active components and potential mechanisms of synergy are still unclear. The objective of this study was to develop a method that combines bionic technology, plant metabolomics and network pharmacology to discover the active components and potential mechanisms underlying the enhanced therapeutic effects of VCR for PD. Vinegar processing alters the flavor of C. rotundus, leading to changes in its properties. The acidic nature of vinegar enhances the selectivity of the medicine toward the liver, thereby improving its ability to soothe the liver, regulate qi and provide pain relief. Through gas chromatography-mass spectrometry and multivariate statistical analysis, 30 key differential components between raw C. rotundus and VCR have been screened and identified. These differential components primarily exert their therapeutic effects in treating PD by modulating targets such as interleukin-6, TNF, TP53 and PTGS2, as well as pathways including the estrogen signaling pathway, ovarian steroidogenesis, the TNF signaling pathway and the HIF-1 signaling pathway. The findings of this study serve as a reference for the application of VCR in compound formulas and clinic practiceal. Furthermore, the methodology employed in this study provides research insights for the processing of other Chinese medicines.

Ling Gui Zhu Gan decoction (LGZGD) is a traditional Chinese medicine (TCM) prescription that is widely used in cardiovascular disease clinical prevention and treatment with high efficacy. Recent studies have shown that LGZGD can also be used in hyperlipidemia (HL) intervention, but its pharmacodynamic material basis and its mechanisms remains unclear. This study aimed to reveal the protective effects of LGZGD on HL, elucidate the pharmacodynamic material basis. The hamster HL model was established by high-fat diet. Thereafter, non-targeted metabolomics and quantitative lipidomics were established for screening differential metabolites and pathways. Finally, the mechanisms were elucidated based on network pharmacology to screen for shared targets, which were computational selected by molecular docking. After four weeks of LGZGD administration, the TC, TG, and liver index levels decreased notably and hepatocyte injury was obviously reduced. The Multi-omics identified 62 differential metabolites and 144 differential lipids, respectively. The network pharmacology study predicted 343, 85, and 974 relevant targets from LGZGD components, HL, differential metabolites and lipids, respectively. Eventually, seven core targets were selected by molecular docking. Six key components in LGZGD, including genistein and naringenin, could play a therapeutic role in HL by regulating seven pathways, including HMGCR and PPARA. This comprehensive strategy provides a promising example and approach for further research on TCM for the treatment of lipid metabolic diseases.

目的 增加葛根作为淡豆豉发酵辅料,探讨葛根参与淡豆豉发酵对体外抗骨质疏松活性的影响.方法 采用SPSS 22.0软件比较分析加葛根纯种菌发酵组、无葛根纯种菌发酵组、仅葛根辅料纯种菌发酵组、加葛根自然发酵组、无葛根自然发酵组和仅葛根辅料自然发酵组的药效活性,同时对比纯种发酵组1～7 d 的最优发酵时间.结果 抗骨质疏松作用最好的为加葛根纯种发酵 4 d的淡豆豉,在1×10-4 g·L-1 浓度下促成骨细胞增殖作用最佳,增殖率可达45.8%.结论本研究证实葛根作为辅料发酵淡豆豉可有效提高体外抗骨质疏松活性,为传统中药发酵提供了新思路.

Cyanotis arachnoidea C. B. Clarke is a traditional Chinese medicinal herb that has a limited clinical use in the treatment of diabetes mellitus (DM) in minority areas of Guizhou in China. However, few prior reports are available on the quality control of Cyanotis arachnoidea, and its quality markers and hypoglycemic mechanism are still unclear. The purpose of this study is to explore the quality markers (Q-markers) of Cyanotis arachnoidea and predict its hypoglycemic mechanism. In this study, ultra-high-performance liquid chromatography (UHPLC) fingerprint combined with chemical pattern recognition were performed, and four differential components were screened out as quality markers, including 20-Hydroxyecdysone, 3-O-acetyl-20-hydroxyecdysone, Ajugasterone C, and 2-O-acetyl-20-hydroxyecdysone. Network pharmacology analysis revealed 107 therapeutic target genes of Cyanotis arachnoidea in DM treatment, and the key targets were Akt1, TNF, IL-6, MAPK3, and JUN. The hypoglycemic mode of action of Cyanotis arachnoidea may be mediated by tumor necrosis factor (TNF) signaling, cancer, insulin resistance, and JAK-STAT pathways. Molecular docking analysis disclosed that the foregoing quality markers effectively bound their key target genes. An in vitro experiment conducted on pancreatic islet β-cells indicated that the forenamed active components of Cyanotis arachnoidea had hypoglycemic efficacy by promoting PI3K/Akt and inhibiting MAPK signaling. UHPLC also accurately quantified the quality markers. The identification and analysis of quality markers for Cyanotis arachnoidea is expected to provide references for the establishment of a quality control evaluation system and clarify the material basis and hypoglycemic mechanisms of this traditional Chinese medicine (TCM).

Objective: To establish an ultra-high performance liquid chromatography-tandem mass spectrometry（UPLC-MS/MS） method for the simultaneous determination of twenty chemical components（arecoline hydrobromide, adenosine cyclophosphate, neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, liquiritin, benzoylmesaconine, benzoylaconitine, isoliquiritin, benzoylhypacoitine, liquiritigenin, isoliquiritigenin, glycyrrhizic acid, 6-gingerol, honokiol, costunolide, dehydrocostus lactone, magnolol, betulinic acid）in Shipi powder. Methods: The separation was performed over 40.0 min on a Shim-pack GIST C 18 column（100 mm×2.1 mm, 2.0 μm） with a mobile phase consisting of 0.1% formic acid in water and acetonitrile with gradient elution（0-12 min, 2%B→14%B;12-24 min, 14%B→51%B;24-26 min, 51%B→70%B;26-36 min, 70%B;36-37 min, 70%B→2%B;37-40 min, 2%B） at a flow rate of 0.3 mL·min -1 . The column temperature was 35 ℃ and the injection volume was 1 μL. The detection was carried out by electrospray ionization（ESI）. Positive and negative electrospray ionizations were performed in multiple reaction monitoring（MRM） mode. Results: All of the analytes showed good linearity（r≥0.997 6） in the tested ranges. The precision, repeatability and stability of the method were good for the twenty chemical components. The average recoveries were in the range of 93.4%-108.7% with relative standard deviations RSD≤5.0%. The contents of arecoline, adenosine cyclophosphate, neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, liquiritin, benzoylmesaconine, benzoylaconitine, isoliquiritin, benzoylhypacoitine, liquiritigenin, isoliquiritigenin, glycyrrhizic acid, 6-gingerol, honokiol, costunolide, dehydrocostus lactone, magnolol, betulinic acid in 15 bantches of in Shipi powder were 7 190.26-13 726.74, 90.11-301.93, 9.65-71.13, 102.88-710.98, 40.51-179.73, 10.90-71.22, 3 424.43-27 337.63, 508.34-1 276.14, 108.27-192.65, 95.74-378.92, 565.19-1 018.53, 106.23-623.42, 3.53-16.47, 6 104.50-15 933.93, 1 047.58-1 750.15, 537.91-2 582.74, 332.83-581.28, 348.22-760.81, 350.65-2 189.73, 2.06-39.22 μg·g -1 . Conclusion: The established method is accurate, stable and reproducible, and can provide a research method for the quality control of Shipi powder.

加强中医药大学专业课课程思政,有利于提高中医药人才培养质量.充分挖掘无机化学课程中思政元素,大力推动课程思政,让学生不止掌握专业,更是认同专业,热爱自己的专业,激发学生为中医药事业发展贡献自己力量的决心,这才是课程思政应该发挥的作用.绪论对于学生正确认识一门课,爱上一门课发挥着很重要的作用.本文探讨如何在无机化学绪论部分有效地引入课程思政,后续将深入探究其他章节中的思政元素.

彝族药蜘蛛香(Valeriana jatamansi Jones)中含有多种化学成分,主要为环烯醚萜类、挥发油类、木脂素类和黄酮类.据现代药学研究表明,蜘蛛香有多种药理作用,如中枢抑制作用、抗菌和抗病毒作用、细胞毒性和抗肿瘤作用等.本文基于中药质量标志物的概念,从蜘蛛香化学成分特有性、传统药效、传统药性、化学成分可测性等几个方面,对蜘蛛香的中药质量标志物成分进行预测分析,以期为蜘蛛香的质量控制和合理应用提供新思路.

目的 建立同时测定旋覆代赭汤中15 种成分含量的超高效液相色谱-质谱联用(UPLC-MS/MS)方法.方法 采用Shim-pack GIST C18(2.1 mm×100 mm,2.0 μm)色谱柱,流动相用乙腈-0.1%甲酸,梯度洗脱,流速 0.3 mL·min-1,柱温35℃.采用ESI源,以MRM负离子模式检测.结果 甘草苷、甘草酸、甘草素、异甘草素、异甘草苷、芹糖异甘草苷、新甘草苷、绿原酸、隐绿原酸、阿魏酸、异绿原酸A、异绿原酸C、人参皂苷Rb1、人参皂苷Re和6-姜辣素在各自浓度范围内线性关系良好(r＞0.998 6),平均回收率在97.0%～103.1%,精密度、重复性、稳定性均符合要求.结论 建立的方法简单、准确性好,可为旋覆代赭汤及其制剂的质量控制提供参考.

目的:建立石楠叶药材HPLC-PDA指纹图谱,结合化学计量学识别和筛选石楠叶药材的质量标志物,为石楠叶的质量提供科学依据.方法:采用Agilent Eclipse XDB-C18色谱柱(250 mm×4.6 mm,5μm);以乙腈(A)-0.1％甲酸水溶液(B)为流动相,梯度洗脱,体积流量1.0 mL·min-1,检测波长350 nm,柱温35℃;建立15批石楠叶药材指纹图谱并进行相似度评价,对共有峰进行化学识别模式分析区分不同产地的石楠叶药材,筛选石楠叶的质量标志物.结果:15批石楠叶药材的相似度均在0.996以上,共标定了10个共有峰,聚类分析、主成分分析和偏最小二乘分析筛选出1号色谱峰(新绿原酸)、2号色谱峰(绿原酸)、4号色谱峰(芦丁)、5号色谱峰(金丝桃苷)、6号色谱峰(异槲皮苷)为石楠叶的质量标志性成分,其中新绿原酸、绿原酸的含量与药材的优质性呈正相关,芦丁、金丝桃苷和异槲皮苷的含量可能与药材质量呈负相关,说明这3种成分在药材中含量不宜太高.进一步对以上5个成分进行定量分析,线性范围分别为:0.1353～2.7060,0.1340～2.6800,0.0085～0.1705,0.0156～0.3128,0.0059～0.1189μg,各成分线性关系良好(R2≥0.9990),平均回收率在99.30％ ～101.90％.结论:建立的HPLC指纹图谱及多成分含量测定方法稳定、可靠,可为石楠叶药材的质量评价提供依据.

露水草是鸭跖草科蓝耳草属一种多年生草本植物,属于彝族药的一种,植物蜕皮甾酮是露水草的主要活性成分之一,目前已鉴定出植物蜕皮甾酮、植物甾醇、植物甾酮苷、脂肪酸、萜及挥发油等多种化学成分,其中植物蜕皮甾酮的含量最为显著.植物蜕皮甾酮具有广泛的生物活性,在抗炎、抗肿瘤、保护神经系统、降低血糖血脂、心血管、骨骼系统以及器官保护等方面作用显著.本文总结了露水草中植物蜕皮甾酮类化学成分及其生物活性,为露水草的深度开发利用提供参考.

Cyperus rotundus (CR) is the dry rhizome of the Cyperaceae plant Cyperus, which is widely used in food processing, such as curry, pickling spices, and baking goods, as well as medicinally after stir-frying. The level of stir-frying (slightly stir-frying, stir-frying yellow, and stir-frying black) has a direct impact on the flavor, aroma, and pharmacological effects of the food. However, the distinctions between various levels of stir-frying have not been studied. The purpose of this study was to develop a method that combines electronic tongue, electronic nose, and HS-GC-MS to elucidate the flavor and aroma differences between three types of stir-fried CR and to identify aroma difference components. As the degree of stir-frying increased, the sweet, bitter, astringent, and umami flavors of the sf-CR diminished while the aroma intensified. Among the 33 differential compounds, pyrazines, D-limonene, camphene, trans-verbenol, and ponocarvone were the most significant aroma-causing components. This method effectively differentiates the three levels of sf-CR and provides a foundation for the use of sf-CR in food processing and pharmaceutical applications.

目的 采用超高效液相色谱串联飞行时间质谱(UPLC-Q-TOF-MS)法快速分析石楠Photinia serrulata叶化学成分.方法 色谱柱为Waters BEH C18色谱柱(100 mm×2.1 mm,1.7 μm),流动相为乙腈(A)-0.1％甲酸水溶液(B),梯度洗脱,体积流量0.3 mL/min.采用peakview 1.2软件解析样品和混合对照品质谱图,得到化合物的精确相对分子质量、多级质谱数据、质谱碎片离子信息等,结合文献报道进行成分鉴定.结果 共鉴定了 98个化学成分,主要包括30个黄酮类成分,24个有机酸类成分,15个苯丙素类成分,14个萜类成分和15个其他类成分,其中95个化合物是首次从该植物中发现的化学成分.结论 建立的UPLC-Q-TOF-MS法能快速、准确、较全面地鉴定石楠叶药材化学成分,为石楠叶药效物质基础研究提供依据.

目的:建立15批旋覆代赭汤物质基准的特征图谱和含量测定方法.方法:通过HPLC法测定15批旋覆代赭汤物质基准特征图谱和人参皂苷Rg1、人参皂苷Re、甘草苷、甘草酸、6-姜辣素、芦丁的含量,采用"中药色谱指纹图谱相似度评价系统(2012版)"和SPSS 21.0软件对特征图谱进行评价.结果:15批旋覆代赭汤样品共标记了17个共有峰,指认了 7个成分,相似度为0.882～0.998.聚类分析可将15批样品聚为3类.上述6个成分的平均加样回收率分别为96.46％、98.93％、97.26％、102.83％、97.91％、101.57％.以含量均值上下浮动30％规定含量范围未出现离散数据.结论:建立的特征图谱和含量测定方法简便、稳定、准确,可为旋覆代赭汤物质基准的质量研究提供参考.