The early diagnostic methods for non-small-cell lung cancer (NSCLC) are limited, lacking effective biomarkers, and the late stage surgery is difficult and has a high recurrence rate. We investigated whether the effects of FBXO45 in arcinogenesis and metastasis of NSCLC. The up-regulation of FBXO45 expression in NSCLC patients or cell lines were observed. FBXO45 gene promoted metastasis and Warburg effect, and reduced ferroptosis of NSCLC. FBXO45 induced ZEB1 expression to promote Warburg effect and reduced ferroptosis of NSCLC. Sh-FBXO45 reduced cancer growth of NSCLC in mice model. FBXO45 decreased the ubiquitination of ZEB1, leading to increased expression of ZEB1, which in turn promoted the Warburg effect and reduced ferroptosis in NSCLC. In vivo imaging, Sh-FBXO45 also reduced ZEB1 expression levels of lung tissue in mice model. FBXO45 in NSCLC through activating the Warburg effect, and the inhibition of ferroptosis of NSCLC by the suppression of ZEB1 ubiquitin, FBXO45 may be a potential therapeutic strategy for NSCLC.

Non-small-cell lung cancer (NSCLC) is the major subtype of lung cancer, with a series of long non-coding RNAs (lncRNAs), microRNAs (miRNAs), and proteins involved in its pathogenesis. This study sought to investigate the functionality of lncRNA EPB41L4A antisense RNA 1 (lncRNA EPB41L4A-AS1) in the proliferation of NSCLC cells and provide a novel theoretical reference for NSCLC treatment. Levels of lncRNA EPB41L4A-AS1, miR-105-5p, and GTPase, IMAP family member 6 (GIMAP6) in tissues and cells were measured by RT-qPCR and the correlation between lncRNA EPB41L4A-AS1 and clinicopathological characteristics was analyzed. Cell proliferation was evaluated by cell counting kit-8 and colony formation assays. The subcellular localization of lncRNA EPB41L4A-AS1 was analyzed by the subcellular fractionation assay and the binding of miR-105-5p to lncRNA EPB41L4A-AS1 or GIMAP6 was analyzed by dual-luciferase and RNA pull-down assays. Functional rescue experiments were performed to analyze the role of miR-105-5p/GIMAP6 in NSCLC cell proliferation. lncRNA EPB41L4A-AS1 and GIMAP6 were downregulated while miR-105-5p was upregulated in NSCLC tissues and cells. lncRNA EPB41L4A-AS1 was correlated with tumor size and clinical staging and its overexpression reduced NSCLC cell proliferation. lncRNA EPB41L4A-AS1 was negatively correlated with miR-105-5p and positively correlated with GIMAP6 in NSCLC tissues, and lncRNA EPB41L4A-AS1 sponged miR-105-5p to promote GIMAP6 transcription in NSCLC cells. Overexpression of miR-105-5p or knockdown of GIMAP6 reversed the inhibition of lncRNA EPB41L4A-AS1 overexpression on NSCLC cell proliferation. lncRNA EPB41L4A-AS1 was downregulated in NSCLC and mitigated NSCLC cell proliferation through the miR-105-5p/GI-MAP6 axis.

目的 分析平滑肌22 alpha蛋白(SM22α)、转化生长因子-β1(TGF-β1)在结直肠癌组织中的表达水平,并探讨其与肿瘤侵袭转移的关系.方法 回顾性选取咸阳市中心医院2018年4月至2019年5月期间收治的104例结直肠癌患者为研究组,按照肿瘤侵袭转移情况分为侵袭转移组(n=31)和非侵袭转移组(n=73),并选取同期于我院接受治疗的87例良性结直肠肿瘤患者作为对照组.采用免疫组化S-P法检测并比较各组患者结直肠病变组织中SM22α、TGF-β1表达水平,采用Spearman秩相关分析SM22α、TGF-β1表达水平与肿瘤侵袭转移的关系,并采用受试者特征曲线(ROC)评估SM22α和TGF-β1表达水平对结直肠癌患者肿瘤侵袭转移的评估效能.结果 研究组患者的SM22α阳性表达率为48.08％,明显低于对照组的64.37％,TGF-β1阳性表达率为69.23％,明显高于对照组的36.78％,差异均有统计学意义(P<0.05);C期/D期患者SM22α阳性表达率为32.61％,明显低于A期/B期的60.34％,TGF-β1阳性表达率为80.43％,明显高于A期/B期的60.34％,差异均有统计学意义(P<0.05);侵袭转移组患者的SM22α阳性表达率为32.26％,明显低于非侵袭转移组的54.79％,TGF-β1阳性表达率为87.10％,明显高于非侵袭转移组的64.38％,差异均有统计学意义(P<0.05);经Spearman秩相关分析结果显示,SM22α与肿瘤侵袭转移呈负相关(r=-0.471,P<0.05),TGF-β1与肿瘤侵袭转移呈正相关(r=0.543,P<0.05);ROC分析结果显示,SM22α、TGF-β1及联合检测肿瘤侵袭转移的ROC曲线下面积分别为0.663、0.690、0.731,检测灵敏度分别为80.3％、78.6％、84.5％,特异性分别为60.8％、60.0％、61.8％,并且联合检测的ROC曲线下面积高于单项检测,检测灵敏度和特异性高于单项检测.结论 结直肠癌组织中SM22α表达减少,TGF-β1表达增加,且与肿瘤侵袭转移密切相关,可用于初步诊断肿瘤侵袭转移,且两者联合检测诊断效能更好.

Objective To investigate the expression and clinical value of the E-selectin gene ( SELE ) in colorectal cancer (CRC). Methods Using gene expression profiles and clinicopathological data for patients with CRC from The Cancer Genome Atlas, and tumor and adjacent normal tissues from 31 patients with CRC from Xianyang Central Hospital, we studied the correlation between SELE gene expression and clinical parameters using Kaplan–Meier and Cox proportional hazards regression analyses. Results Higher expression of SELE was significantly associated with a poorer prognosis and shorter survival in patients with CRC. The median expression level of SELE was significantly higher in CRC tissues compared with healthy adjacent tissue. Cox regression analysis showed that the prognosis of CRC was significantly correlated with the expression of SELE . Immunohistochemical analysis also showed that positive expression of E-selectin increased significantly in line with increasing TNM stage. C onclusion: This study confirmed that SELE gene expression is an independent prognostic factor in patients with CRC.

目的:分析17例小汗腺汗孔瘤(EP)患者的临床表现、病理学特点及诊断、鉴别诊断.方法:对我院近8年明确诊断EP的患者进行临床资料分析,观察HE及免疫组化、PAS染色.结果:小汗腺汗孔瘤多见于四肢末端,表现多样,16例患者临床均误诊,镜下见肿瘤由增生一致的基底细胞样立方形细胞构成,界限清,从表皮下层向下延伸至真皮内,可见有单层细胞覆盖的有嗜酸性护膜的导管分化或正在形成导管的空泡化细胞及少数囊腔和角质小皮,瘤团周围无栅栏状排列,与基底细胞癌、脂溢性角化病及透明细胞汗腺瘤难以鉴别.结论:小汗腺汗孔瘤是一种少见的良性附属器肿瘤,易误诊,确诊必须依靠病理检查,手术单纯切除即可治愈.

目的:研究转录因子Snail及上皮钙黏素E-cadherin在乳腺浸润性导管癌中的表达,探讨二者与乳腺浸润性导管癌各临床病理因素之间的关系,并分析二者的相关性.方法:应用免疫组织化学SP法检测55例患者乳腺浸润性导管癌组织与癌旁组织中Snail及E-cadherin的表达情况.结果:E-cadherin在实验组中的阳性表达率为58.18％,在对照组中阳性表达率为98.18％,两者差异有统计学意义(P<0.01),E-cadherin的表达与患者年龄、乳腺浸润性导管癌组织学分级、HER-2相关.Snail在实验组中的阳性表达率为87.27％,在对照组中阳性表达率为12.73％,两者比较差异有统计学意义(P<0.01).Snail的表达与乳腺浸润性导管癌组织学分级、ER、HER-2相关.由癌旁组织到乳腺浸润性导管癌组织中,E-cadherin的阳性表达呈递减,而Snail的阳性表达呈递增,且E-cadherin和Snail的表达呈负相关(r=-0.341,P<0.01).结论:E-cadherin及Snail是患者乳腺浸润性导管癌发生、进展以及转移的重要生物学标志,与肿瘤的发生及肿瘤的浸润转移密切相关.

患者女性,16岁,2个月前无明显诱因出现咳嗽、咯痰,痰液量多,为白色黏稠痰液,伴气喘、气短明显,活动后加重、声音嘶哑,无发热、胸痛、恶心、呕吐等症状.查体:精神差,喘息貌,甲状腺缺如,双肺呼吸音粗,可闻及广泛喘鸣及少许湿鸣音,其余未见明显异常.1年前因甲状腺囊肿于外院行手术切除甲状腺,术后病理诊断:甲状腺乳头状癌,术后并发甲减,长期服用优甲乐治疗.复查CT示:双侧甲状腺区未见明显甲状腺,环状软骨后方类圆形软组织密度影,其与食管上端分界不清,同平面气管腔轻度狭窄,腔内凹凸不平.胸廓入口处气管左侧淋巴结肿大.双肺炎症,右肺支气管腔团块状高密度影.右侧局部胸膜增厚,气管狭窄明显.气管镜检查:声门下约1 cm处可见占位性病变,堵塞气道约95％,表面有渗血,肿瘤灰白色,质软(图1),大部分清除,术后病理送检.

患者女性,44岁,无明显诱因上腹部胀痛不适1周,疼痛无放射,伴恶心、呕吐,无发热、咳嗽、心悸及气促等症状.查体:体温36.9 ℃,心率80次/min,呼吸18次/ min,血压100/70 mmHg,急性病容,限制呈屈膝蜷卧位,上腹部膨隆.3年前因阴道不规则出血,诊断为子宫颈癌,在外院行子宫颈癌根治性切除术,术后行盆腔手术区域放射性治疗及化疗6次.CT检查示:子宫呈术后改变,余盆腔CT增强无明显异常,十二指肠与空肠交界处管腔较窄,左肾下极小结石,肝胆胰脾CT增强无明显异常.

目的 探讨幽门螺杆菌(Hp)感染与咸阳地区上消化道疾病的关系.方法 应用W-S银染和革兰染色法检测上消化道疾病患者病理组织切片中Hp的感染状况,并分析Hp感染与上消化道疾病及人群分布的关系.结果 咸阳地区上消化道疾病患者Hp感染率为64.61％(115/178),其中浅表性胃炎、消化性溃疡、癌前病变及胃癌组织中Hp感染率分别为49.12％、60.71％、70.37％、77.27％.胃癌组与浅表性胃炎组比较Hp感染率比较差异有统计学意义(P＜0.05);农村居民与城镇居民Hp感染率有差异(P＜0.05).不同性别上消化疾病患者Hp感染率无差异.结论 咸阳地区上消化道疾病患者Hp感染与上消化道疾病病变程度有关,农村居民Hp感染率高于城镇居民.

目的:探讨增生性外毛根鞘囊肿(proliferating trichilemmal cyst,PTC)的临床病理学特征、诊断及鉴别诊断、组织发生.方法:对10例PTC病例进行临床资料分析、光镜观察并复习相关文献.结果:10例均发生于成年人,女性6例,男性4例,平均年龄58岁(37-83岁).大体上,肿瘤位于皮肤真皮深层及皮下组织,结节状,境界清楚.镜下PTC最重要病理特征为增生的鳞状上皮细胞形成小叶团块及其中央的外毛根鞘型角化,常伴有外毛根鞘囊肿.免疫组化:p53灶状阳性.结论:PTC为真皮及皮下结节,一般呈良性经过.良、恶性PTC的区分应该结合临床和病理学特征.鉴别诊断包括皮肤鳞状细胞癌和外毛根鞘癌等.p53阳性提示PTC为低度恶性肿瘤,应注意密切随访.

患者男,62岁.2年前因颈后包块行局部手术切除,术后临床诊断为"脂肪瘤",未做病理检查.3个月后局部复发一无痛性渐增性包块,至2012年7月10日入院时如拳头大.彩超示包块内回声不均,有少许血流信号.

患者男,62岁.2年前因颈后包块行局部手术切除,术后临床诊断为脂肪瘤,未做病理检查.3个月后局部复发一无痛性渐增性包块,至2012年7月10日入院时如拳头大.彩超示包块内回声不均,有少许血流信号。

患者女,50岁.左前臂包块20余年于2010年4月入院.包块近年渐增大,无明显不适,表面皮肤无异常改变,肿块无压痛,可推动.临床诊断为脂肪瘤,遂行脂肪瘤切除术.术中见肿块位于皮下,与周围组织分界清楚,易剔除,表皮未见累及.

患者女,50岁.左前臂包块20余年于2010年4月入院.包块近年渐增大,无明显不适,表面皮肤无异常改变,肿块无压痛,可推动.临床诊断为脂肪瘤,遂行脂肪瘤切除术.术中见肿块位于皮下,与周围组织分界清楚,易剔除,表皮未见累及。

Objective: To investigate the clinicopathologicl MRI characteristics,differential diagnosis,treatment and prognostic of malignant solitary fibrous tumor of the meninges.Methods: One case of malignant SFT of the meninges was reported,the clinical features,immunohistochemical analysis and imaging appearance were observed.Results: The patient was a 65-year-old male presenting with four limbs were weakness,the memory was bad,MRI showed the left temporoccipital lobe approximately 8.5cm×6.5cm×6cm cystic solid mass.In operation was the tumor attached to the meninges of discovered obviously,the tumor was well circumscribed with the periphery brain organization.General sample showed grayish white isotropic shape,and cystic changes.Histopathologically: The tumor was composed of the spindle-shaped cells with fascicular,stroform or patternless pattern,disposed in sfascicular arrangement and separated by prominent eosinophilic bands of collagen,and showed a haemangiopericytoma-like structure.the nuclear mitoses(4 /10HPF),no obvious necrosis.Immunochemistry results showed the tumor cells were diffusely positive for vimentin,CD34,CD99,bcl-2.ki-67 labelling index was more than 10%.The pathological diagnosis was malignant SFT of meninges.Conclusion:The malignant SFT of meninges is very rare.Diagnosis is based on its pathological morphology and immunohistochemistry.Differential diagnosis includes other spindle-shaped celltumors of the meninges.The treatment is complete surgical resection,and a long-term follow-up is required.

Objective:To investigate the cause of Kikuchi's lymphadenitis and clinical characteristics.Methods:Thirty one cases with Kikuchi lymphadenitis,were analysed for the clinical features.Results:Among 31 cases,14 male,17 female.High fever and superficial lymph nodes can be seen in all cases also lymphocyte apoptosis nuclear debris;numerous polymorphical histiocyte proliferation in focal or lange areas were visualized.CD45,CD68 and CD3 were positive in 31 cases.Conclusion:Lymphnode focal necrosis and histiocytic proliferation are featares of Kikuchi's lymphadenitis.CD68 and CD3 expression has very important value for HNl's diagnosis.The disease is self-limited disease,most patients have good prognosis.

Purpose To study the clinicopathological characteristics of pleomorphic hyalinizing angiectatic tumor of soft parts(PHAT).Methods The clinical,pathoglogical and immunohistochemical features of six cases of PHAT were analyzed.The literature was reviewed.Results All cases occurred in adults with an equal gender distribution.The average age was 44 years(range 24~64 yrs).The tumors were located in the lower extremities(n=3),neck(n=2),and retroperitoneum(n=1).The main clinical presentation was a painless mass with duration ranging from 3 months to 38 years.Grossly,the tumors were well circumscribed,measuring 4.3 cm in mean diameter.They showed grayish,gray to tan color on cut surface.In 2 cases,hemorrhagic cysts of varying size were noted.Microscopically,the tumor was characterized by clusters of thin-walled ectatic vessels lined by thick hyalinizing fibrin-collagen material,with sheet-like or fascicular proliferation of spindled to pleomorphic cells between or around the vessels.Mitotic figures were rare,however,nuclear pseudoinclusions were prominent.Immunohistochemically,the neoplastic cells were positive for vimentin,with partial expression of CD34 and CD99,but negative for S-100 protein,desmin,α-SMA and CD31.MIB-1 proliferative activity was lower than 2%.Follow-up data showed no recurrence after complete excision in all 6 cases.Conclusions PHAT is a rare soft tissue neoplasm with low grade malignant potential.Recognizing its characteristic features will facilitate the diagnosis and differential diagnosis.Complete resection with negative margins remains the mainstay of treatment.Long-term surveillance is required.