目的观察黄芩苷对视网膜母细胞瘤HXO-RB44细胞增殖活性、凋亡指数、侵袭性及PCNA、CAS-3蛋白表达水平的影响,并探讨其可能机制。方法将对数生长期HXO-RB44细胞制成单细胞悬液接种于96孔培养板中,分为对照组及50,100,200μg/m L黄芩苷组并给予相应剂量药物处理,MTT法测各组细胞增殖抑制率,流式细胞术测细胞凋亡指数,Transwell侵袭小室实验测细胞侵袭能力,Western Blot法检测PCNA、CAS-3蛋白表达情况。结果 MTT结果显示黄芩苷对HXO-RB44细胞增殖有明显抑制作用,且呈时间依赖性;流式细胞术结果显示黄芩苷各组中HXO-RB44细胞凋亡指数显著高于对照组;Transwell侵袭小室实验显示黄芩苷能明显降低HXO-RB44细胞的侵袭能力,均呈剂量依赖性;Western blot显示随着黄芩苷浓度的增加,PCNA蛋白表达逐渐下降,而CAS-3蛋白表达增加,呈剂量依赖性。结论黄芩苷可抑制视网膜母细胞瘤细胞增殖,诱导细胞凋亡,并抑制其侵袭能力,其可能是通过调控PCNA、CAS-3蛋白的表达,激活相关细胞凋亡信号通路来完成的。

目的：观察应用高压氧综合治疗脑外伤后动眼神经损伤的临床疗效。方法：98例脑外伤后动眼神经损伤患者随机分为观察组（高压氧联合药物治疗组）48例和对照组（单纯药物治疗组）50例，比较两组患者的治疗效果。结果：①观察组痊愈39例，有效 8例 ，总有效率为97．9％；对照组痊愈31例，有效15例，总有效率为92．0％。两组患者痊愈率与总有效率比较差异显著（P ＜0．01）。②两组患者复视、睑下垂、瞳孔散大、眼球运动症状改善时间比较有显著性差异（P＜ 0．01）。结论：应用高压氧综合治疗脑外伤后动眼神经损伤效果理想，值得推广应用。

Objective To study the percutaneous permeability of low molecular weight heparin sodium by Azone with rat skin in vitro. Methods The turbidimetric technique was used to determine the low molecular weight heparin sodium. Rat skin was used as permeation barrier. Permeation studies in vitro were carried out by using Franz-diffusion cells to investigate the cumulative permeation content of low molecular weight heparin sodium. Results 1.0%-5.0% Azone could enhance the percutaneous absorption of low molecular weight heparin sodiumin. Conclusion Azone can be used as transdermal enhancers for low molecular weight heparin sodium intransdermal drug delivery formulations.

目的:研究糖尿病性视网膜病变(DR)在不同阶段形态改变时视网膜振荡电位(OP)的动态变化,为DR的早期诊断及病程进展预测提供客观的检测依据.方法:对确诊的2型糖尿病患者用检眼镜检查进行筛选,以眼底荧光素血管造影(FFA)进行明确分期,对各期病人在3年内定期行糖化血红蛋白测定(6次)、行视网膜震荡电位检查(2次),最后1次检测配合眼底荧光素血管造影检查.结果:视网膜振荡电位(OP)总波幅降低幅度< 10.64±4.94uv(约<7.29%),可作为预测视网膜病变未进展的参考值;OP总波幅下降幅度>31.62±9.73uv(约>22.23%),则可作为预测2型糖尿病人发生DR或DR发生进展的依据.视网膜振荡电位(OP)总波幅降低程度与糖尿病视网膜病变病程进展有密切相关性.结论:OP的监测可预测DR的发展.

AIM:To study dynamic changes of glycosylated hemoglobin and retinal oscillatory potentials in diabetic retinopathy(DR) .·METHODS:Ophthalmoscope examination was used to screen patients with typeⅡ diabetes. In this study,clear phases was limited by fundus fluorescein angiography(FFA) . Each patient of three-year period regularly measured glycosylated hemoglobin six times and the retinae potentials two times. The final tests were combined with FFA,and the results were analyzed.·RESULTS:When HbA1c level was controlled between 4.99%±0.58%,it could be used as the safety reference value for prevention of occurrence and development of retinopathy;HbA1c level continued in 9.77%±1.57%,the progress of DR speeded up significantly;the total amplitude of retinal oscillatory potentials(Op) decrease <10.6±4.9μV might be taken as stable reference value;amplitude of Op overall decrease >31.6±9.7μV might be used to predict the occurrence of DR or DR progression.·CONCLUSION:Combination of HbA1c and Op can predict the development of DR.

AIM:Comparison of simple excision of pterygium,combined excision of pterygium with stem cell transplan-tation of corneal limbus and combined stem cell transplan-tation of corneal limbus with recombinant human epidermal growth factor derivative eye drops(rhEGF) . Evaluate the curative effect and compare the curative and recurrent situation among three operative methods.·METHODS:One hundred and twenty cases(121 eyes) were divided into three groups according to operative method,half a year later,observe the curative and recur-rent as well as the corneal situation. The data was dealt with SPSS 13.0.·RESULTS:The repair time of epithelium in the group with rhEGF was lower than those of the other two groups,cure rate increased and recurrence reduced remarkably(P<0.05) .·CONCLUSION:Combined excision of pterygium with stem cell transplantation of corneal limbus and rhEGF,an effect method to cure pterygium,can reduce the recurren-ce and lower the repair time of epithelium.

AIM: To evaluate the efffects of HeXue MingMu Pian for retinal hemorrhage caused by ventinal vein occlusion and diabetic vetinopathy.METHODS: A random controlled study was performed on 68 patients of retinal hemorrhage caused by ventinal vein occlusion and diabetic vetinopathy. They were assigned into 3 groups randomly: 24 patients in A treatment group (34 eyes), 24 patients in B control group (34 eyes), 20 patients in C control group (28 eyes). Took orally HeXue MingMu Pian in A group, 5 tablets every time, 3 times every day , 15 days every period of treatment, at intervals of 5 days, for 3 periods of treatment . Puerarin injection 400mg taken into 5% glucose or normal saline injection was given by intravenous drip to B group,once a day, 14 days every period of treatment, at intervals of 6 days, for 3 periods of treatment. Radix Salviae Miltiorrhizae 250ml was given by intravenous drip to C group, once a day, 14 days every period of treatment, at intervals of 6 days, for 3 periods of treatment. Visual acuity, visual fi eld and fundus fluorescein angiography condition were examined to evaluate the effects of treament. The main subjects of A,B,Cgroups before treament were comparable.RESULTS: In A group, 14.7% were completely recovered , 38.24% were obviously improved, the total rate of effectiveness was 85.29%. In B group, 11.76% were completely recovered, 35.29% were obviously improved, the total rate of effectiveness was 82.35%. In C group, 10.71% were completely recovered, 23.53% were obviously improved, the total rate of effectiveness was 60.71%. The difference of effective rate between A and B was no signifi cant(p>0.05).The difference of effective rate between A and C was significant(p<0.05). The difference of effective rate between B and C was significant(p<0.05).CONCLUSION: HeXue MingMu Pian is an effective drug for retinal hemorrhage caused by ventinal vein occlusion and diabetic vetinopathy and is taken conveniently and reliably.

AIM:To discuss the effect of Pingyangmycin in treating pterygium at early stage.· METHODS:Eighteen cases(26 eyes)with pterygium were treated with 1mg Pingyangmycin by local injection and received 1g/L ofloxacin combined dexamethasone eyedrops after injection.The intraocular pressure(IOP),cornea and lens were observed before and after injection.· RESULTS:Among 26 eyes of 18 cases,the pterygium of 24 eyes(92%)gradually fell off,the development of pterygium in 2 eyes(8%)stopped,and a little corneal fluorescein stain was found in 7 eyes(27%)the next day,and disappeared 3 days later,without any complications such as intraocular hyperten-sion and cataract.· CONCLUSION:Pingyangmycin for treating pterygium at early stage by local injection is proved to be a simple,safe,feasible and effective way.

Objective To study the relationship of intraocular pressure (IOP)and myopia in children and teen-agers.Methods 230 patients ranged from 8 to 18 years old were divided into four groups:emmetropia(- 0.25D～+0.25D),low myopia(-0.50D～-2.75D),medium myopia(-3.00D～-5.75D ),severe myopia(≥6.00D).The IOP and diopter were tested and statistically analyzed.Results 1.That the IOP of severe myopia is the highest,and it has a significant difference between the severe myopia and the other three groups.2.The number of female is more than male in severe myopia.Conclusions The IOP is obviously high in severe myopia.It is necessary that the IOP of severe myopia was checked regularly and dropped down.

AIM: To discuss the relationship of diopter, axial length and intraocular pressure (IOP) for teenagers with myopia, and to evaluate the effect of genetic and environment factors. ·METHODS: Corneal diopter, axial length and IOP were exactly measured on 329 eyes of 166 teenagers with myopia. They were divided into four groups according to the corneal diopter: emmetropia group, mild myopia group, moderate myopia group and high myopia group. SPSS13.0 was used to analyze the data.·RESULTS: There was remarkable difference of diopter and axial length between moderate and high myopia (P<0.05). A significantly positive correlation between the diopter and axial length was found in four groups(P<0.05). It is considered that corneal diopter and IOP had positive correlation in moderate and high myopia (r=0.817,P=0.031), and corneal diopter of the teenagers was correlated with their parents. ·CONCLUSION: Axial length and IOP is correlated with the corneal diopter of teenagers. The increase of axis is considered to be a major factor for teenage myopia, and heredity and environment are two important factors that influence the formation of myopia.

AIM:To detect the antigen-gene of HLA-B27 in the patients with acute anterior uveitis (AAU) by microcytotoxic assay and compare the clinical symptoms between HLA-B27 positive and negative patients group . · METHODS: HLA-B27 in 100 AAU patients was detected by microlcytotoxity assay and 20 patients from both positive and negative group were choosed randomly to campare their clinical characteristics. · RESULT: In all 100 AAU patients, 64 patients were HLA-B27 positive and 36 were negative. Comparision in the 20 patients of two groups showed HLA-B27 positive patients were characterized by their predilections for the male, single eye, severe visual impairment with white KP, easy recurrence, many complications and sensitive to steriod. · CONCLUSION: Detecting HLA-B27 in the patients with AAU is helpful to evaluate the cause and the prognosis of AAU. There is significant difference in clinical characteristics between HLA-B27 positive and negative patients.

目的分析新生血管性青光眼的手术疗效.方法对20例(23眼)新生血管性青光眼,术前充分控制全身病(高血压、高血糖等),最大限度降低眼压,在粘弹剂辅助下行小梁切除术,术后辅以5-氟尿嘧啶及按摩治疗.结果术中所有患者前房均有少量出血,但均在3 d内自行吸收.术后大部分患者前房维持良好.术后20眼视力维持术前,3眼略有提高;眼压降至21 mmHg(1 kPa=7.5 mmHg)以下者20眼;虹膜新生血管变细或消失19眼.结论对新生血管性青光眼在小梁切除术中辅以粘弹剂,术后配合5-氟尿嘧啶及按摩治疗,效果肯定,并发症少.

目的:观察复方樟柳碱注射液联合葛根素注射液对缺血性视神经视网膜脉络膜病变的疗效.方法:采用随机研究方法,将缺血性视神经视网膜脉络膜病变的患者108例随机分为对照组(50例),治疗组(58例).对照组采用妥拉苏林注射液25mg,治疗组采用复方樟柳碱注射液2mL,均做患眼颞侧浅动脉旁皮下注射,1次/d,疗程为30d.两组均联合葛根素注射液400 mg静脉点滴共30d.以视力、视野、眼底改变为疗效判断指标.对照组与治疗组治疗前各项主要指标具有可比性(P＞0.05).结果:有效率治疗组88.9%,对照组49.1%,两者比较其差异有显著性意义(x2检验,χ2=20.140,P＜0.001).结论:复方樟柳碱联合葛根素注射液对缺血性视神经视网膜脉络膜病变疗效显著.

目的探討阿托品和雙星明在青少年散瞳驗光中的應用價值.方法對82例青少年屈光不正患者,按年齡分為5歲～12歲及12歲～18歲兩組,均行阿托品和雙星明雙重散瞳前後電腦驗光,對其進行對比分析.結果 (1)球鏡值:散瞳前後電腦驗光,兩種藥物、兩組病人之間均有顯著差别;阿托品與雙星明之間又有顯著差别,P值均小于0.05.(2)柱鏡值:散瞳前後電腦驗光,兩種藥物、兩組病人之間,阿托品與雙星明之間均無顯著差别,P＞0.1.(3)柱鏡軸:散瞳前後或使用不同藥物,散光值＜1.50D的患者,電腦驗光檢測,柱鏡軸均有較大差别;散光值≥1.50D的患者,電腦檢測其軸位则較準確.結論 對于患有遠視或近視的青少年,仍以阿托品散瞳驗光較為準確.對于單純散光患者,則無需散瞳.

目的探讨翼状胬肉切除术前及术后角膜曲率的改变.方法随机选择鼻侧胬肉40例,分为2组.侵犯角膜≤2.5 mm为第1组,15例;≥3 mm为第2组,25例.两组均行术前及术后视力、角膜曲率和电脑验光检测,进行对比分析.结果视力:第1组术前术后相差0.09,第2组0.23.角膜曲率:垂直径均大于水平径,其差值:第1组术前术后相差0.77KS,第2组4.46 KS.散光值:2组均为远视散光,第1组散光值术前术后相差+0.4 DC,第2组+3.3 DC.结论胬肉可使角膜曲率改变.胬肉侵犯角膜≤2.5 mm时,切除胬肉视力提高不明显;侵犯角膜≥3 mm时,切除胬肉视力明显提高.

无脉症是一种罕见的主动脉弓及大动脉的慢性进行性疾病。因其供血障碍，常发生一系列眼部症状，严重影响视力甚至失明，往往首诊于眼科。作者近年遇到 2例报道如下： 1 临床资料例 1，女， 27岁。以双眼视力下降 1年，加重 1个月收入院。检查：贫血貌。阵发性晕厥以致不能坚持检查。桡动脉搏动消失，颈动脉搏动微弱，足背动脉搏动可触及。血压上肢测不出。视力：右眼指数 /10cm，左眼 0.01。双眼球结膜血管呈黑暗红色，迂曲扩张，虹膜基质萎缩，新生血管形成。眼底：双眼视神经乳头界清，有新生血管。视网膜动脉细，静脉扩张迂曲，粗细不均。视网膜散在出血、微血管瘤及棉毛样软性渗出斑。左眼颞上枝静脉距乳头部 3pd处呈壶腹状扩张，继而变细且与动脉形成吻合。诊断：无脉症 (合并症期 )，转内科。 例 2，女， 20岁。以“视网膜静脉周围炎”收入院。检查：双上肢血压测不出，下肢血压 24/13kPa。桡颈动脉搏动消失，足背动脉搏动可触及。眼科情况：视力：右手动 /30cm，左 0.1。双眼球结膜血管迂曲扩张，右玻璃体浑浊，可见机化条索，眼底看不清。左玻璃体轻混。眼底：视神经乳头界尚清，网膜静脉迂曲，广泛点状及片状出血，视网膜周边血管有闭塞，视乳头上及视网膜微血管瘤增生，视乳头周围可见动静脉吻合。诊断：无脉症 (大动脉炎，头臂动脉型 )。处理：用皮质类固醇，血管扩张剂，中药 (抗凝剂－ 797)等治疗后，视力无明显好转，全身症状改善而出院。 2 讨论 无脉症为一罕见疾病，多见于青年女性，发病年龄多在 20～ 30岁左右。其病因不明，发病机理不清楚，可能属于胶原性疾病。主要病变位于主动脉弓和从它发出的动脉，如无名动脉、颈总动脉或锁骨下动脉等。动脉发生瘢痕化的广泛性动脉炎，使管腔闭塞或血栓形成，造成大脑、眼部、面部以及上肢的血供障碍。其特点：桡、颈动脉搏动消失，上肢血压不能测出。进行性视乳头周围动静脉血管互相形成吻合，并发白内障，语言及听力障碍等 [1]。本病属进行性，预后不良。常由于并发白内障、青光眼、视网膜脱离、眼球萎缩等而失明；颈动脉血栓性闭塞，发生脑部缺血或心肌病而死亡。无特效疗法，采用中西医结合治疗，可暂时改善病情，如本文例 2。故作者认为，对目前所知，容易引起本病的许多原因，如风湿，结核以及最常侵犯年轻妇女的弥漫性非特异性动脉炎，颈动脉炎，胶原病等均应及早诊治以免引起本病。