目的 探讨干扰MS4A8对苦参碱抑制胃癌细胞生长的影响.方法 构建干扰MS4A8细胞株,将所有细胞分为对照组、苦参碱组、苦参碱+shMS4A8组.采用CCK-8法检测各组细胞的增殖活性,细胞克隆实验检测细胞克隆形成,流式细胞仪检测各组细胞的凋亡情况,Western blot检测各组细胞中P13K-Akt-mTOR信号通路相关蛋白[磷酸化细胞外调节蛋白激酶(p-ERK)、磷酸化氨基未端蛋白激酶(p-JNK)、磷酸化P38丝裂原活化蛋白酶(p-p38)]和MAPKs信号通路相关蛋白[胞内磷脂酰肌醇激酶(PI3K)、磷酸化蛋白激酶B(p-Akt)、雷帕霉素靶蛋白(mTOR)]表达情况.结果 与对照组相比,苦参碱组细胞增殖率降低(LSD-t=6.93,P=0.045);与苦参碱组相比,苦参碱+shMS4A8组的细胞增殖率更低(LSD-t=6.34,P=0.005).与对照组相比,苦参碱组AGS细胞克隆数明显减少(LSD-t=3.33,P=0.045);与苦参碱组相比,苦参碱+shMS4A8组的细胞克隆数更少(LSD-t=3.03,P=0.005).与对照组相比,苦参碱组AGS细胞凋亡率增加(LSD-t=-7.76,P=0.0004);与苦参碱组相比,苦参碱+shMS4A8组的细胞凋亡率更高(LSD-t=-16.66,P=0.0001).与对照组相比,苦参碱组p-ERK、p-JNK、p-p38、PI3K、p-Akt、mTOR的相对表达量均减少(LSD-t=5.95,P=0.0117;LSD-t=9.43,P=0.0027;LSD-t=11.41,P=0.0016;LSD-t=27.10,P=0.0002;LSD-t=34.75,P=0.0001;LSD-t=13.89,P=0.0010);与苦参碱组相比,苦参碱+shMS4 A8 组 p-ERK、p-JNK、p-p38、PI3K、p-Akt、mTOR 的相对表达量均更少(LSD-t=5.41,P=0.0115;LSD-t=7.08,P=0.0044;LSD-t=4.11,P=0.0284;LSD-t=10.18,P=0.0011;LSD-t=4.22,P=0.0262;LSD-t=10.26,P=0.0011).结论 干扰 MS4A8 可通过 P13K-Akt-mTOR和MAPKs信号通路增强苦参碱抑制细胞增殖和细胞克隆数形成,增加细胞凋亡.

虎尾轮为闽南民间草药,民间广泛用于治疗急慢性胃炎、肺系疾病、跌打损伤等.从本草学角度及临床应用作一概述,为综合开发利用虎尾轮提供依据.

目的 建立薄层色谱法及高效液相色谱法测定栀子根、茎、枝中齐墩果酸含量的方法,对栀子根、茎、枝中齐墩果酸的含量进行比较.方法 将采集的栀子根、茎、枝样本处理后,采用薄层色谱法定性检测样本齐墩果酸成分;采用HPLC法测定样本中齐墩果酸的含量,并对数据进行综合分析处理.结果 薄层色谱法定性分析栀子根、茎、枝中均含有齐墩果酸成分;HPLC法含量测定栀子根、茎、枝3个部位中齐墩果酸的含量具有显著差异,其含量顺序为根>茎>枝.结论 栀子根、茎、枝中均含有齐墩果酸成分,栀子茎齐墩果酸含量较栀子根相对接近,可作为拓宽栀子根药材药用部位进一步研究的基础依据.

目的:考察不同干燥方式对栀子根中4种环烯醚萜苷类成分含量的影响.方法:采用晒干、阴干、不同烘干时间(8、12、18、24h)及不同烘干温度(60、70、80℃)等14种方法对样品进行处理.运用HPLC法测定栀子根中山栀苷、去乙酰车叶草酸甲酯、羟异栀子苷、鸡矢藤次苷甲酯的含量;采用单因素方差分析和TOPSIS法对数据进行综合分析.结果:不同干燥方式对4种成分含量影响显著;综合评价结果表明不同干燥方式排序为80℃12 h＞自然晒干＞80℃18 h＞自然阴干＞70℃24 h＞80℃8 h＞60℃18 h＞60℃12 h＞60℃8 h＞70℃18 h＞60℃24 h＞80℃24 h＞70℃8 h＞70℃12 h.结论:于80℃干燥12h为栀子根最优干燥方法,可为栀子根的产地加工提供借鉴.

目的 上巳菜品名多且混乱,本文拟通过本草考证正本清源上巳菜品名.方法 通过本草文献学考证方法检索相关资料并整理.结果 上巳菜为福建民间常用中草药,民间临床多用于治疗急慢性肾脏疾病、糖尿病、高血压、各种出血症状,效果显著,然因历史变革及地区习惯不同,上巳菜名称多且混乱,本文通过对上巳菜品名的本草学考证及临床使用考证,为上巳菜综合开发利用提供依据.结论 上巳菜药用植物名为荠菜,民间常将其作为野菜及青草药使用.

目的 分析牛至和贯叶金丝桃原植物及饮片的鉴别特征,找出能快速有效区别这两个品种的鉴别手段.方法 比较牛至和贯叶金丝桃在原植物性状、饮片特征、有效成分等存在的区别点,进行性状鉴别和理化鉴别.结果 牛至和贯叶金丝桃虽然颇为相似容易混淆,但通过抓住它们的区别点仍可快速鉴别出.结论 掌握有效的性状和理化鉴别法有助于快速鉴别易混淆中药.

吴茱萸外敷辅助治疗疾病,效果显著.本文通过吴茱萸在儿科应用的几个案例分析吴茱萸外敷用于治疗小儿疱疹性咽峡炎,急性淋巴结炎,感染性发热及小儿腹泻的临床应用原理及相关药性研究.

Gardeniae fructus acts on clearing heart fire,eliminating damp and cooling blood,detoxifi-cation.It is the effective herb for reducing fire of sanjiao,removing heat and heat of the liver and gallbladder, cooling blood and detoxification.It is extensively used in clinical practice from the ancient time to the pres-ent.But gardeniae fructus is bitter and cold herb and easily injures the spleen and stomach. The ancient scholar pays the great attention on the processing and proper application of it in clinical practice.In the pa-per,the literatures on the different processing methods,the medical scholars′discussion on the processing methods and the clinical precautions were analyzed so as to provide the evidence of gardeniae fructus in clini-cal application.The study results show that gardeniae fructus is apt to injure the spleen and stomach due to its bitter in taste and cold in nature and to damage yin due to its action of resolving dryness.The contraindica-tions of gardeniae fructus are qi deficiency of the spleen and stomach,deficiency cold syndrome,poor appe-tite,loose stool and yin and blood deficiency.It is especially contraindicated in yin excess,separation of yin from yang or the syndrome of true cold and false heat.This herb should be used carefully in clinical practice.

Objective To investigate the use of SoPhorae Tonkinensis Radix et Rhizoma in our hos-Pital to Provide the scientific reference for safe and rational use in clinic. Method 495 PrescriPtions con-tained with SoPhorae Tonkinensis Radix et Rhizoma were selected from Xiamen hosPital of TCM in 2005. These PrescriPtions were analyzed retrosPectively by EXCEL. Results The research showed a equal ProPor-tion of men and women in using SoPhorae Tonkinensis Radix et Rhizoma. The age mainly distributed in 20 to 60 years of age(87. 87% )and the dePartment mainly distributed in the dePartment of gastroenterology (73. 33% ),followed by the dePartment of rheumatology and nePhrology and encePhaloPathy. The TCM syn-drome mainly contained with hyPeractive liver - qi attacking the stomach,damP heat in the sPleen and the stomach and wind - heat affecting lung. Conclusion The utilization of SoPhorae Tonkinensis Radix et Rhizo-ma as a common traditional Chinese medicine in our hosPital is basically reasonable.

本研究借鉴中草药提高人体免疫力的研究成果,通过分子生物学技术,测定中草药补肾治疗前后华南虎血清细胞因子IL-2、IL-2R、IL-6、IFNγ;血清免疫球蛋白指标IgG、IgA、IgM及超氧化歧化酶SOD、谷胱甘肽过氧化物酶GSH-Px等生化指标,探讨中草药补肾治疗提高老龄华南虎免疫功能及抗氧化功能的机理,制定提高华南虎免疫力的中草药配方,为制定华南虎保护策略和遗传管理计划提供参考.

The medicine of She ethnic group originates from practice, and the people of She ethnic group are good at dietotherapy.The medicated diet and dietotherapy of She ethnic group has the characteristics of prevention as primary, paying attention to benefiting and invigorating, making good use of fresh product, and so on.It is not only used to enhance physical fitness, prolong life, but also for the treatment of various acute and chronic diseases, and the effect is significant.This paper introduced the characteristics of medicated diet and dietotherapy of She ethnic group, and looked forward to the prospect of medicated diets.

目的：通过调查我院门诊中药饮片处方情况，规范处方管理，促进临床安全、合理、有效、经济地使用药物。方法随机抽取2013年1月～12月间我院的1200张中药饮片处方（均由每月16日随机抽取100张），从中筛选不合理处方，进行归类点评分析。结果1200张处方中不合格处方103张，处方合格率为91.4%。结论我院存在一定程度的不合理用药现象，主要是由于医生的工作态度及个人习惯所致，少部分是专业知识的欠缺。定期坚持做好处方点评，有助于提高我院门诊中药处方的质量。