The morbidity of cardiovascular disease is globally high and frequency.It is of great sinification for the improvement of curative effect and reduction of the incidence of medical accidents.People are paying increasingly attention on the intensification of quality of nursing.

Objective To find out the risk factors and the controlling strategy of hospital infection.Methods Analyse the reasons of incompetent.Results It is useful for the control of hospital infection to intensify the management of the sanitize of the air of the ward,nurse appliances and hands of hospital staff.Conclusion Make sure each step is under control,fulfil the policy of monitoring.

2002年1月～2007年3月,我们共收治假肥大型肌营养不良(DMD)患儿15例.现分析如下.

近年来,我们收治了52例糖尿病肾病患者,经精心护理,效果满意.现将护理体会报告如下.

Objective To isolate H.pylori mouse-passage strains. Methods (1) H.pylori strains carrying cagA genes and vacA genes were inoculated into stomach of 10 BALA/c mice.4 weeks after the model was made,the stomach biopsies of all the mice were collected to assess the colonization of H. pylori. The H. pylori Mouse-passage strains (litter 1,litter 2) were obtained successfully. (2) The colonization of H.pylori was assessed by bacterial culture inspection. Results 3 BALA/c mice were inoculated orally through a feeding tube with 4 isolated H. pylori carrying cagA genes and vacA genes successfully. The infected rate of H. pylori was 33.3% (3/9), two strains were transferred successfully.H.pylori was located mainly above gastric pit and less in number. Conclusion The Yunnan H. pylori strains carrying cagA genes and vacA genes can locate in the stomach of BALA/c mice after being given orally into mice'stomach in which the H.pylori mouse-passage strains can be cultured.

甲硝唑因对幽门螺杆菌(H.pylori)有较好的抗菌作用而被用于联合根除治疗方案,但临床滥用甲硝唑、患者依从性差以及初始治疗失败等因素使其产生了全球性的耐药问题,其发生率呈迅速上升趋势.虎月燕等[1]的调查发现云南地区H.pylori对甲硝唑的原发耐药率为67.98%.有鉴于此,本研究从天然药物着手,以期筛选新的抗H.pylori天然药物以用于甲硝唑耐药产生后的补救治疗,为今后的临床应用研究打下基础.

目的 探讨臭灵丹对幽门螺杆菌(H.pylori)甲硝唑耐药株及敏感株的体外抑制作用.方法 琼脂稀释法检测H.pylori甲硝唑耐药株和敏感株对臭灵丹水煎剂的最低抑菌浓度(MIC),并计算MIC50、MIC75、MIC90 .结果 臭灵丹对H.pylori的MIC值范围为3.125～200mg/ml,H.pylori甲硝唑耐药株和敏感株对臭灵丹水煎剂的敏感性无统计学差异(相对中位数潜力1.153,95%的可信区间为0.84～1.602),臭灵丹对H.pylori甲硝唑耐药株和敏感株的MIC50分别为21.2mg/ml和18.8mg/ml,MIC75分别为42.4mg/ml和36.9mg/ml,MIC90分别为77.9mg/ml 和67.8mg/ml.结论 臭灵丹有良好的抑菌效果,H.pylori甲硝唑耐药株及敏感株都对其敏感.

Objective To study in vitro the inhibition effect of Pomegranate Rind on H.pylori metronidazole resistant strains and susceptible strains. Methods The minimal inhibitory concentration (MIC) of the water extract of Pomegranate Rind on H.pylori metronidazole resistant strains and susceptible strains was detected by the agar dilution method, MIC50MIC75MIC90 were also calculated. Results MIC of Pomegranate Rind on H.pylori was in a range from 7.8125 mg/ml to 500 mg/ml. The susceptibility of H.pylori metronidazole resistant strains and susceptible strains on Pomegranate Rind had no statistical difference (relative median potential = 1.072, 95% confidence interval from 0.763 to 1.513), MIC50 was 29.9 mg/ml and 28.0 mg/ml respectively, MIC75 was 65.1 mg/ml and 59.1 mg/ml respectively, MIC90 was 131.1 mg/ml and 115.9 mg/ml respectively. Conclusion H.pylori metronidazole resistant strains and susceptible strains were susceptible to Pomegranate Rind.

经内镜取出上消化道异物在消化内镜诊疗工作中并不罕见.吞入异物的原因多样,异物的种类各异,取出异物的技巧也不相同.近半年来,本消化科内镜室所诊治的异物较集中表现为长条形金属物.

Objective To study the distrbuition characteristics of interleukin-1 genotype and the relation between interleukin-1 genotype and the infection and density of helicobacter pylori in Yunnan region. Methods The infection and density of helicobacter pylori were investigated from the gastric mucous membrane samples;The genotypes of interleukin-1 beta-511 (IL-1B -511 )、interleukin-1 beta+3954 (IL-1B +3954 ) 、interleukin-1 receptor antibody (IL-1RN) from peripheral blood were determined by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).Results Among interleukin-1 beta-511 (IL-1B -511 ) genotypes, There were C/C genotype 24 cases (17.3%)、C/T 75 cases (54.0%)、T/T 40 cases (28.8%); among interleukin-1 beta+3954(IL-1B +3954 ) genotypes, There were C/T genotype 7 cases (5.0%)、C/C genotype 132 cases (95.0%)、no T/T ; Interleukin-1 receptor antibody (IL-1RN) genotypes, There was 1/4 、2/2 genotype each 1 case、1/1 110 cases (79.2%)、1/2 27 cases (19.4%). IL-1B -511 */T genotype is predisposing factor for helicobacter pylori infection,OR value is 6.1. In patients with age above 60 years old, IL-1B -511 */T genotype is risk factor for helicobacter pylori density,OR value is 8.0.Conclusion There was difference in the clinical results related to helicobacter pylori-infection between people in east and in west. IL-1B -511 */T genotype was related to the extra high H.pylori detection rate in patients with DU, this genotype could easily result in severe infection of helicobacter pylori.

Objective To find the best combination of Laggera pterodonta, Pomegranate Rind, Taraxacum mongolicum for H.Pylori metronidazole resistant strains. Methods The best combination of Natural Chinese herbs for anti- H.Pylori metronidazole resistant strains was screened by Orthogonal Test Method. Results The best combination scheme of the three Natural Chinese herbs on H.Pylori metronidazole resistant strains was Laggera pterodonta (MIC90, 77.9 mg/ml), Pomegranate Rind (MIC90, 131.1 mg/ml), Taraxacum mongolicum (MIC75,117.27 mg/ml). Conclusion Orthogonal Test Method can enhance the efficiency to screen the best combination of three medical herbs, and is of time saving and resource saving.

高血压脑出血在急性出血性脑血管疾病中较常见,发生率高,预后差、死亡率及致残率高,特别是出血量大,血中破入脑室的,而传统的治疗方法大多为内科保守治疗 .随着影像与立体定向技术的广泛应用,高血压脑出血的手术治疗方法日趋增多.我院自2003年10月～2004年3月对46例高血压脑出血患者采用微创穿刺颅内血肿碎吸术治疗,取得了满意的效果.现报道如下.

2000年8月-2002年12月,我院共完成电子结肠镜检查3585例,其中检出结肠憩室126例,检出率3.51%.报告如下.

1998年1月～2002年12月,我院共完成电子结肠镜检查5700例,其中检出鞭虫病30例,检出率5‰,报告如下.

1997年2月2003年2月,我们为上消化道大出血患者行急诊内镜检查,对21例证实为非静脉曲张性上消化道大出血患者进行胃镜下注射止血,取得满意疗效.

1998年1月至2002年12 月,我院共完成电子结肠镜检查5 700例,其中检出鞭虫病30例,检出率5‰,报告如下: 临床资料:30例中男7例,女23例.年龄22～67岁,平均39岁.职业:农民21例,工人2例,个体户4例,干部1例,演员1例,乡卫生员1例.就诊原因:慢性右下腹疼痛11例,其中4例伴腹泻,2例伴粘液血便;反复鲜血便6例;全腹隐痛3例;慢性便秘3例;便后肛门疼痛4例;肛门坠胀感或腹泻便秘交替3例.

2000年1月～2002年1月,我院共完成电子胃镜检查9100例,其中检出食管孤立性静脉瘤96例,检出率1.05%,报告如下.

1 病例报告 例1,患者,女,13岁,因反复腹部隐痛3年,大便带血半年余到我院就诊.查体:轻度贫血貌,口周及双侧手指、脚趾有深褐色和黑色点状色斑,表面光滑,余无特殊.

幼年性息肉是引起小儿下消化道出血的常见的病因之一,我院于1991-2000年应用成人结肠镜高频电凝切除小儿结肠息肉36例,均无出血,穿孔等并发症.1 资料及方法1.1 临床资料本组36例,男22例,女14例,年龄3-12岁,平均7岁,患儿均表现为间歇性便血,其中5例伴有腹痛,病程最短2月,最长3年.36例中单发性息肉30例,多发性息肉6例,息肉最大3.5cm,最小0.8cm,息肉位于直肠13例(16颗),乙状结肠21例(25颗),降结肠2例(2颗).