介绍林下栽培大球盖菇技术,包括高产培养料配方(木屑30％、玉米芯30％、稻壳30％、虫粪沙10％),一行双拱的菇床铺设方式,二次播种,覆土打孔,以及菌丝期、菇蕾期、幼菇期的温度、湿度、光照管控技术要点,采用该技术模式栽培,每潮菇出菇时间均可保持在一周左右,每亩每潮产量约4 000 kg.

为筛选出适合平顶山地区保护地种植的优质、高产小果型新品种西瓜,引进了9个小果型西瓜品种进行品种筛选试验.结果筛选出早红蜜、超越梦想、京颖、小兰等几个综合表现较好的适合平顶山地区保护地种植的品种.

本文作者通过对品种选择、催芽、播种、育苗等技术的介绍,为苦瓜早春日光温室栽培打好基础.定植后的肥水管理、整枝吊蔓和对病虫害的防治等关键技术的利用,为苦瓜早春日光温室高产栽培奠定了坚实的基础.

通过引进6个蝴蝶兰品种进行筛选试验,比较其生长开花特性,并结合市场认同度综合评价,筛选出适合平顶山地区种植和推广的品种,为广大花卉种植户提供参考.

通过开展蝴蝶兰商品花催花试验，比较6个品种蝴蝶兰在平顶山地区催花的效果，调查其花芽分化时间、开花时间、双芽率和花芽大小等数据，得出这6个品种催花方面的特性，结合市场对这些特性的要求，为本地区的生产栽培管理者提供品种选择方面的参考依据。

With ethanol reflux extraction leek seed active ingredient, product yield and adenosine content as the index, to optimize the extraction technology research, the results show that the material liquid than 1∶6, 4 h, 70% ethanol concentration, extraction time, pH value of 6.0, in this condition, the product yield is around 14%, adenosine content is above 400 mg/kg.

温室条件生长的蝴蝶兰，对温度、肥料、光照有一定要求，通过研究这些条件对蝴蝶兰生长的影响，总结蝴蝶兰温室栽培的管理要点，为广大蝴蝶兰生产及管理人员提供参考。

In order to study on the corresponding relationship between ISSR markers and heterosis of leek ,this paper calculated the heterosis of tiller number and other traits ,and compared the of heterosis of tiller number ,plant weight and sheath length with ISSR genetic distance of the parents based on the investigation of the traits of 27 hybrid combinations of leek and their parents .The inquiry elicited the strong heterosis was mostly concentrated in the position of genetic distance over 5 .0 .

综述了韭菜的育种理论、种质资源的引进和整理、育种方法的研究进展，探讨了韭菜育种的方向及发展趋势。

The seed oil of Allium tuberosum is extracted by supercritical CO2 fluid extraction, and the result shows that granule sizes 50~60 mesh, extraction pressure 22~25 MPa, extraction temperature 45℃, extraction time 2.5 h and CO2 flow rate on the production 3.0 L/h, after investigating the ifluences of above-mentioned three factors on oil yield, which reached above 17%under the feasible operating conditions.

通过分析不同浓度的DNA模板、引物、dNTP、MgCI2、Taq酶、温度、时间及循环次数等各因素对扩增效果的影响,总结了韭菜ISSR-PCR扩增效果的影响因子及对策,提出了改进措施,为开展韭菜ISSR-PCR扩增技术研究提供参考。

从繁殖方法、管理技术、病虫害防治等方面介绍了温室花卉仙客来的栽培技术,以为仙客来的栽培提供参考。

In order to provide theoretical information for leek breeding,this study was to probe genetic diversity of the leek germplasms.96 leek samples were used to detect the intra-and inter-specific genetic variation by inter-simple sequence repeat(ISSR) analysis.The result showed that 20 ISSR primers were screened from 77 primers based on 96 leek samples.The first leek DNA fingerprint library was constructed by PCR amplification,PAGE fingerprint detection,and fingerprint data collection of Gel imaging.The ISSR amplification diversity reached 94.7%,which was an effective molecular marker.The genetic similarity(GS) among 96 leek samples ranged from 0.58 to 0.88,which could fully show that they have rich genetic diversity.The result of cluster analysis showed that the cluster analysis correlated with sample origin and was consistent with species distribution.The breeding and spreading cultivars with relatively low diversity clustered more together,which means the new leek breeding innovation should introduce germplasms from home and abroad base.

鄂梨1号和鄂梨2号在豫中南地区表现早丰性强、甜度高、风味佳,但病虫害较严重.在无公害栽培管理上,介绍了建园、整形修剪、土肥水管理、花果管理、病虫害防治和果实采收等技术.

A total of 174 accessions of leeks germplasm collected from Germplasm Repository were used in this study.According to the clustering result of 15 phenotypic traits,all the accessions were divided into 14 categories when the genetic distance was 5.3.Based on combination of simple ratio method and artificial,we established a primary core collection containing 69 accessions of leeks germplasm,which accounted for 40% of the whole collection.Compared with the whole collection,the genetic diversity index of primary core collection was higher than that of initial accessions except for plant sheath length and leaf width.Between core collection and initial collection,the mean difference percentage was 0,the variation coefficient rate was 106.14%,and coincidence rate for range was 94.43%.The significant correlation was found between the leaf thickness and other traits,and the leaf number and other traits were respectively missed two traits in the primary core repository.The other traits correlations were consistent with the traits correlations in total repository.The results showed that the genetic variation and genetic structure of the accessions can be well preserved in this primary core collection.

PAGE技术是以聚丙烯酰胺凝胶作为载体的一种区带电泳检测技术,包括控制聚丙烯酰胺凝胶浓度、聚合速度、均匀度、着色度、干净度和凝胶漂移等技术环节。在分析各技术环节影响因素的基础上,系统地总结了各技术环节对韭菜ISSR扩增产物电泳指纹检测效果的影响。

针对甘薯长期大田种植,病毒侵染,种性退化,抗性降低,产量和品质下降的特点,提出对甘薯进行脱毒,恢复其优良种性,增强抗病能力,达到高产优质的目的。

<正>韭菜传统栽培方式是10月收获种子,翌年春季播种,经当年营养生长和根株越冬,下年才能抽薹结实,从收获种子至抽薹结实需要3a(年),时间较长,

研究了基于ISSR-PCR扩增的DNA指纹检测韭菜种子纯度的方法。从发芽种子的嫩芽提取基因组DNA,筛选6种ISSR引物进行ISSR-PCR扩增。聚丙烯酰胺凝胶电泳检测表明,用ISSR-64引物检测平丰8号,韭菜种纯度约92%。该技术具有准确性高、时效性好、检测成本较低等特点。