目的 探讨去氢木香内酯对喉癌细胞增殖、迁移和侵袭的影响,以及对磷脂酰肌醇-3 激酶(phosphatidyl inositol 3 kinase,PI3K)/蛋白激酶 B(protein kinase B,Akt)信号通路的调节作用.方法 第 4 代对数生长期 Hep-2 细胞经不同浓度(0、10、20、30μmol/L)去氢木香内酯处理后,MTT 法检测细胞增殖抑制率、流式细胞仪检测细胞凋亡率、Transwell实验检测细胞迁移和侵袭能力,蛋白印迹法检测 p-PI3K、p-Akt、Sox-2、CD44、CD133 和 Nanog蛋白相对表达量.结果 与 0μmol/L 组比较,10、20 和 30μmol/L组细胞增殖抑制率和细胞凋亡率均升高(P＜0.05),迁移和侵袭能力均减弱(P＜0.05),p-PI3K、p-Akt、Sox-2、CD44、CD133 和 Nanog蛋白相对表达量均降低(P＜0.05),且具有浓度依赖性.结论 去氢木香内酯可抑制喉癌细胞增殖、迁移和侵袭,其可能作用机制是通过抑制 PI3K/Akt信号通路发挥作用.

目的 研究长链非编码RNA含有WW和PDZ结构域2的膜相关鸟苷酸激酶反义RNA 3(Ln-cRNA MAGI2-AS3)在鼻咽癌组织中的表达及与患者放射治疗(放疗)敏感性的关系,并探讨LncRNA MAGI2-AS3对鼻咽癌细胞放疗抵抗的影响及作用机制.方法 采用反转录荧光定量聚合酶链反应(RT-qPCR)检测LncRNA MAGI2-AS3在鼻咽癌组织中的表达水平,分析LncRNA MAGI2-AS3与鼻咽癌患者放疗敏感性的关系.常规培养鼻咽癌细胞CNE2,转染LncRNA MAGI2-AS3小干扰RNA(siRNA),根据是否转染siRNA分为si-NC组和si-LncRNA MAGI2-AS3组.采用CCK8实验检测各组细胞的放疗敏感性;放射处理后,采用平板克隆实验、流式细胞实验、裸鼠实验分别检测各组细胞克隆形成能力、细胞周期、凋亡情况和细胞体内生长能力;采用Western blot试验检测各组细胞周期蛋白D1(cyclin D1)、细胞周期蛋白依赖性激酶4(CDK4)、凋亡蛋白活化的含半胱氨酸的天冬氨酸蛋白水解酶(caspase)3、caspase9表达.结果 RT-qPCR检测结果显示,Ln-cRNA MAGI2-AS3在鼻咽癌组织中表达上调(P<0.05).LncRNA MAGI2-AS3高表达与患者放疗抵抗有关(P<0.05).与si-NC组相比,si-LncRNA MAGI2-AS3组鼻咽癌细胞中LncRNA MAGI2-AS3表达减少、对放疗敏感性增加(P<0.05).放射处理后,si-LncRNA MAGI2-AS3组鼻咽癌细胞克隆形成能力降低(P<0.05),细胞周期阻滞在G0/G1期(P<0.05),细胞凋亡增加(P<0.05),细胞体内生长能力显著降低(P<0.05),cyclin D1、CDK4表达降低,caspase3、caspase9表达增加(P<0.05).结论 LncRNA MAGI2-AS3通过调控细胞周期和凋亡促进鼻咽癌细胞放疗抵抗,是逆转鼻咽癌放疗抵抗的潜在分子靶点.

目的:检测鼻咽癌中微小RNA-221-3p(miR-221-3p)的表达,分析其与增殖、凋亡指标的相关性.方法:选取鼻咽癌患者69例作为观察组,慢性鼻炎患者69例作为对照组.均留取组织标本,应用实时荧光定量聚合酶链反应法检测两组中miR-221-3p的表达.蛋白免疫印迹法检测鼻咽癌中BCL-2相关X蛋白(BAX)的表达.免疫组织化学法检测鼻咽癌中Ki67的表达.原位杂交法检测鼻咽癌中EB病毒的表达.结果:观察组miR-221-3p表达高于对照组,差异有统计学意义(P＜0.05),miR-221-3p的表达在观察组不同组织学亚型、淋巴结转移、局部浸润程度的分组中差异有统计学意义(P＜0.05).生存分析显示miR-221-3p高表达患者的生存时间短(P＜0.05).Spearman相关分析显示,miR-221-3p表达与Ki67增殖指数呈正相关(r=0.56,P＜0.05),与BAX呈负相关(r=-0.69,P＜0.05).结论:与慢性鼻炎患者比较,鼻咽癌中miR-221-3p表达升高.MiR-221-3p高表达促进肿瘤增殖、抑制凋亡,预示病变呈进展状态及较差的预后.

Objective:To study the mechanism of abnormal expression of CDK8 on the cell proliferation, apoptosis and chemosensitivity of human nasopharyngeal carcinoma (NPC).Methods:Cultured cells were divided into three groups:the untreated group (without any intervention), the negative control group (without lentivirus transfection with CDK8 expression) and the transfected group (with lentivirus with CDK8 overexpression), with 12 Petri dishes in each group.Liposomal LipofectamineTM2000 was used to transfect NPC 5-8F cells.The transfection efficiency was verified by Western-blot.The proliferation, apoptosis and cycles of NPC 5-8F cells were analyzed by MTT and flow cytometry (CDK8).Western-blot was used to detect the expression of CDK8 related proteins.The sensitivity of CDK8 to chemotherapeutic agents was detected by MTT.Results:The expression of CDK8 in NPC 5-8F cells transfected with pcDNA3.1/CDK8 was up-regulated (P＜0.05).This indicates that NPC 5-8F cells with stable expression of CDK8 gene were successfully constructed.The abnormal expression of CDK8 in NPC 5-8F cells led to slow proliferation and increase the rate of apoptosis, leading to a significant decrease in the expression of proliferating cell nuclear antigen (PCNA) protein, and the expression level of Cyclin D1 was significantly reduced (P＜0.05).The apoptosis rate significantly increased (P＜0.05).The expression level of multidrug resistance associated protein 1 (MRP1) significantly decreased (P＜0.05).The expression level of Bcl-2 was significantly down regulated (P ＜0.05), and the expression level of Bax significantly increased (P＜0.05).Meanwhile, NPC 5-8F cells significantly increased cisplatin sensitivity (P＜0.05).Conclusion: Abnormal expression of CDK8 can inhibit the proliferation of NPC 5-8F cells and increase the chemosensitivity of cancer cells.It is presumed that the mechanism is related to the down regulating expression of Cyclin D1, PCNA, MRP1 and Bcl-2, and the up-regulate expression of Bax.

目的 探讨Epstein Barr病毒(EBV)编码的microRNA bart7调节鼻咽癌放疗敏感性的机制. 方法 筛选75例鼻咽癌患者以及同期入院体检的30例健康人群为研究对象.比较两组间鼻咽癌患者EBV/DNA比率.测定鼻咽癌患者的microRNA bart7、EBV VCA IgA、EBV EA IgA、EBV Rta IgG等EBV相关肿瘤标志物阳性状态,分析EBV/DNA与microRNA bart7间的相关性. 结果 鼻咽癌患者血浆EBV/DNA比率高于健康人群(92.00％ vs 3.33％,P＜0.05).放疗后鼻咽癌患者血浆EBV/DNA比率低于放疗前(30.67％ vs 92.00％,P＜0.05).放疗后持续缓解的鼻咽癌患者血浆EBV/DNA阳性率低于复发/转移者(12.50％ vs 39.22％,P＜0.05).放疗后鼻咽癌患者血浆microRNA bart7、EBV VCAIgA、EBV EAIgA、EBV Rta IgG阳性率均低于放疗前(P＜0.05).放疗后,持续缓解的鼻咽癌患者血浆microRNA bart7、EBV VCA IgA、EBV EA IgA、EBV RtaIgG阳性率均低于复发/转移者(P＜0.05).EBV/DNA阳性率与microRNA bart7阳性率呈正相关(P＜0.05). 结论 EBV可能通过调节其编码的microRNA bart7水平对鼻咽癌患者放疗敏感性产生影响.

The utility model provides an adjustable angle's rigid nasal endoscope, the angle that can adjust the front end camera lens in the operation as required need not to change the endoscope, and the imagein required region is observed to the in -process of being convenient for to perform the operation, including the epitheca, interior sheath, the objective lens, stereoplasm pull rod and spring, interior sheath cover is established in the epitheca, the epitheca front end is the inclined plane, the objective lens is located inside the epitheca front end, stereoplasm draw -bar box draws together oneand goes up pull rod and a lower link, it is articulated with the peak of objective lens to go up the pull rod, the lower link is articulated with the minimum of objective lens, lower link other endcoupling spring, the spring other end is fixed on locating the fixed block between epitheca and the interior sheath, go up the handle that the pull rod other end was equipped with the perpendicular topull rod, on the epitheca with the position that the handle is relative is equipped with the slot hole, a slot hole lateral wall is equipped with a plurality of draw -in grooves, the high order end and the low order end of objective lens are connected with a revolving axle respectively, the objective lens can rotate in the wraparound pivot.

Background/Aims: The objective of this study was to investigate the potential role of IL-17 in the development of nasopharyngeal carcinoma (NPC) and to screen microRNAs (miRNAs) that potentially target IL-17 in NPC cells. Methods: Blood was collected from NPC patients and normal subjects, and plasma IL-17 concentration was quantified by enzyme-linked immunosorbent assay. An immortalized normal human nasopharyngeal epithelial cell line, NP69, was treated with or without human IL-17 (15 ng/mL) for various times, and expression of IL-1ß, IL-6, IL-12, and TNF-α mRNA was assessed by real-time reverse transcription PCR. The candidate miRNAs that potentially target IL-17 were predicted by a bioinformatics strategy. The selected miR-135a mimic was transfected into primary NPC cells, and cell proliferation was assessed by MTT assay. Results: The concentration of plasma IL-17 was significantly higher in the NPC patients (92.5 ± 7.3 pg/mL) than in the control subjects (56.8 ± 2.9 pg/mL). In response to IL-17 treatment, the mRNA expression of IL-1ß and IL-6 was significantly upregulated and reached a peak at 12 h, followed by a slight decrease at 24 h, while the mRNA expression of IL-12 and TNF-α was significantly upregulated at 12 h and remained high even at 48 h after exposure to IL-17. Moreover, miR-135a specifically targets IL-17 and was dramatically downregulated in NPC cells compared with NP69 cells. Transfection of exogenous miR-135a mimic resulted in significant suppression of IL-17 secretion and subsequent inhibition of NPC cell proliferation. Conclusions: Blood IL-17 was significantly higher in NPC patients compared with normal subjects. Expression of miR-135a in the cancer cells isolated from nasopharyngeal tumors was significantly lower than that in NP69 cells, and suppression of IL-17 by miR-135a mimic resulted in significant inhibition of NPC cell proliferation. These findings suggested that downregulation of miR-135a may contribute to the development of NPC via the mechanism of IL-17 stimulation of proinflammatory cytokine expression.

The utility model provides an eardrum breather pipe erector relates to the medical instrument field, the time can carry in the light source gets into the ear in the operation, makes the operation field of vision better, and direct observation to the intra -aural condition on simultaneously can the outer computer makes operation technique nature better, the technical scheme of the utility model including core bar, sleeve pipe, telescopic handle, the cover pipe box is established outside the core bar, telescopic handle is connected to core bar one end, and other end terminal surface is equipped with LED lamp and image collector, telescopic handle is used for controlling protracting of core bar and contracts with returning, still is equipped with the switch of steerable LED lamp, connection image collector's microcontroller and the wireless communication chip of being connected with microcontroller on the core bar.

The utility model discloses a thyroid gland drag hook of using among tracheotomy, including the connecting rod, and integrative making in the head and the handle at connecting rod both ends, still include and be close to handle one end, and go out the optical cavity on setting up the connecting rod, and be close to head one end, and set up light source chamber on the connecting rod, and set up in the illuminator of light source intracavity side, and connect the intervalve of light source chamber and play optical cavity, illuminator comprises LED pipe, drive circuit and button cell, button cell passes through drive circuit and connects the LED pipe, the LED pipe is just to the intervalve, it comprises non - transparent material to go out optical cavity, intervalve and light source chamber. The utility model discloses a thyroid gland drag hook of using among tracheotomy can guarantee to shine well operation position, and it is simultaneously wild fully to expose the art, and better illumination operation position makes art person easily recognize the operation position, and it is more accurate operate, improves operation efficiency, and it is direct leading -in to the head to avoid illuminator to distribute the heat simultaneously, the improvement nature of performing the operation successfully.

Objective To assess the therapy effect of long-term low-dose clarithromycin joint nasal steroid in the treatment of chro-nic rhino sinusitis. Methods Totally 112 patients with chronic rhino sinusitis who were treated in our hospital from February 2010 to Au-gust 2012 were selected and randomly divided into the treatment group(65) and the control group(47). The patients in the control group were treated by the long-term low-dose clarithromycin joint nasal steroid and nasal irrigation,while the patients in the treatment group were treated by the nasal steroid and nasal irrigation. Then the visual analogue scale, Lund Mackay and the therapeutic effect were evalua-ted. SPSS 16. 0 software was used to analyze the data. Results The Results were as follws:(1) The visual analogue scale score of rhi-nobyon, nasal mucus, headache and hyposmia in the treatment group significantly decreased(P < 0. 05),while only the visual analogue scale score of rhinobyon in the control group significantly decreased(P < 0. 05). (2) The total effective rate in the treatment group (80%) was significantly higher than the control group (51%). The difference was statistically significant(P < 0. 05). (3)About the Lund-Mackey sinus CT score, the treatment group was significantly lower than the control group,and the difference was also statistically significant( P < 0. 05). Conclusion The long-term low-dose clarithromycin joint nasal steroid is superior to nasal steroid in the total effective rate in treatments of patients with chronic rhino sinusitis. It had convenient economic costs and low incidence of adverse reactions. Therefore it has great value for chronic nasal-sinusitis therapy. We recommend it as a first-line drug combination.

The utility model relates to a medical endoscope with self -cleaning function, it includes endoscope installing support and camera lens, the endoscope installing support is telescopic structure and includes inside and outside sleeve pipe, tube hole one end drill way is equipped with the mounting groove in the interior sheathed tube, the outer tube install in endotheca outside of tubes portion and interior sleeve pipe between be formed with liquid holding chamber, inclined plane structure and middle part were equipped with the opening in one of which tip inner bottom was, another tip inner bottom is connected with interior sleeve pipe one end and is closed with interior sleeve pipe one end, be formed with the hydrojet mouth of slope between the notch of an outer tube tip inner bottom and mounting groove, outer tube other end bottom still is equipped with in the regional one side that lies in the holding water cavity and annotates the liquid mouth, annotates the liquid mouth and is connected with external water injection piece. The utility model discloses medical endoscope with self -cleaning function structural design is simple, reasonable, and through the hydrojet mouth that sets up the slope in liquid holding chamber one end, the other end sets up annotates the liquid mouth, can make the endoscope wash in real time in the use, has guaranteed the cleanliness of endoscope.

耳鸣是没有外界刺激条件下患者闻及异常声音的主观感觉，特别是发展到持续性耳鸣时，可使患者产生不安、忧虑、抑郁等心理，严重影响日常生活和工作。耳鸣掩蔽疗法是通过对耳鸣性质的系统测试后，选择与耳鸣音调响度相匹配的特定外界声作为掩蔽声，在医护的指导下聆听掩蔽声以达到抑制耳鸣或缓解耳鸣症状的方法。我科于2011年9月至2014年9月，根据耳鸣诊断标准，收治耳鸣患者85例(129耳)。用 LS-Ⅲ型耳鸣康复仪治疗耳鸣的患者43例(65耳)，治疗情况报告如下。

Objective To understand the relationship between the idiotoxin and airway hyperresponsiveness in elderly patients. Methods Selected 359 cases with allergic rhinitis from February 2013 to February 2014 in our hospital were analysis. Results Methacholine air passage provocation test positive rate was 77%, the normal control group was 16.7%,χ2=28.56, P<0.0001,had difference statistically significance. Conclusion Elderly patients with allergic rhinitis increased airway reactivity, combine with bronchial asthma of high morbidity, early intervention and actively to treat the elderly allergic rhinitis to retard and prevent the occurred of asthma.

目的：探讨同种异体羊膜在中老年鼓膜修补术的临床应用疗效观察。方法将56例中老年鼓膜穿孔患者随机分为两组，在耳内镜下分别采用同种异体羊膜和自体颞肌筋膜行鼓膜修补术，比较两组患者的临床疗效。结果术后随访3～12月，观察两组鼓膜小穿孔的Ⅰ期愈合率分别为92．8％和91．7％，大穿孔的Ⅰ期愈合率分别为92．9％和85．7％，两组患者手术后的平均听阈均有明显提高，且同种异体羊膜组更明显，差异有统计学意义（ P＜0．05）。结论采用同种异体羊膜行鼓膜修补具有成功率高、听力改善明显、取材经济方便、操作简单、组织相容性好、损伤小的特点。

OBJECTIVE:To observe the clinical efficacy of nasal vestibule eczema by using beclomethasone dipropionate in combination with He-Ne laser therapy.METHOD:The 200 cases of nasal vestibule eczema patients were randomly divided into treatment and control groups. The control group received the rub beclomethasone dipropionate cream treatment one time per day. The treatment group supplemented with He-Ne laser irradiation treatment on the basis of the control group on the same treatment, alsoone time per day. Then the results were analyzed.RESULT:The total effective rate was 100.0% in treatment group, while 75.0% in the control group. The difference between the two groups was statistically significant (P < 0.05).CONCLUSION:Beclomethasone dipropionate cream combined with He-Ne laser irradiation therapy on nasal vestibule eczema is significant, and easily to operate, with significant anti-inflammatory, anti-itch, analgesic effects.

儿童食管异物为耳鼻咽喉头颈外科常见急诊之一，其好发于5岁以下儿童［1］。临床表现为哭闹、吞咽困难、流涎、拒食或呕吐等，较大异物若压迫气管，患儿还有发生呼吸困难甚至窒息可能，如诊疗不及时可能引发多种并发症甚至危及生命可能。以往接诊食管异物患儿时，首先均考虑行全身麻醉下硬质食管镜下食管异物取出术，因手术器械质地硬，管径粗加上异物边缘的摩擦作用，术后患儿牙齿磨损、口咽部及食管黏膜损伤常见，食管炎、食管穿孔等发生概率较大，术中手术器械压迫气管，要求行气管插管全身麻醉，由此增加患者术后气道不良反应，术前准备繁琐，治疗费用高，术后住院时间长，患儿痛苦大。本研究主要讨论 Foley管［2］（下称F管）在外形扁平、圆钝光滑类异物取出术中的应用。

<正>变应性真菌性鼻炎-鼻窦炎(allergic fungal rhinosinusitis,AFRS)是真菌性鼻炎-鼻窦炎较为常见的一种类型。AFRS患者有典型的变应性鼻炎的症状及体征,结合鼻窦CT检查显示有病窦以钙化斑或钙化点为典型的影像表现。病理检查可以见到真菌菌丝和孢子柄,可以确诊。AFRS是一种免疫失调性疾病,具有变应性和易复发性,故普通治疗效果往往欠佳。我科2009年1月至2012年7月

<正>Hunt综合征又名耳带状疱疹和膝状神经节炎,是由水痘-带状疱疹病毒感染引起的以侵犯面神经为主的疾病,因Hunt首先描述了该病的临床症状而命名。常见症状为耳部灼痛,起疱疹,面瘫,听力减退及眩晕、耳鸣等。如治疗

目的:探讨电视监视鼻内镜下低温等离子射频消融术治疗儿童腺样体肥大的临床疗效.方法:对90例腺样体肥大患儿采用0°鼻内镜及等离子射频消融治疗系统进行治疗,观察其疗效及并发症.结果:90例术后1～3个月复查临床症状均消失,2例合并鼻窦炎保守治疗2个月后痊愈,随访1年均无复发及并发症.结论:该手术方法视野清晰,操作方便,术中损伤小,无并发症,具有很好的临床应用价值.

鼓膜穿刺是耳鼻喉科在治疗分泌性中耳炎中常用的诊治方法.该治疗方法所需器械各级医院都具有,即75%酒精或0.5%活力碘、1～2%地卡因、棉片、2ml注射器或5号加长鼓膜穿刺针. 方法:常规外耳道消毒麻醉,以左手拇指、食指、中指固定耳廓或耳镜后右手持穿刺针行鼓膜前下象限或后下象限进针,诊断中耳腔内是否有积液或试图吸引出中耳腔内积液.