目的:分析新生儿上呼吸道解脲支原体(UU)和无乳链球菌(GBS)感染的流行病学特征.方法:运用聚合酶链反应技术和传统细菌培养方法检测1206例新生儿的咽拭子标本,分析其发生解脲支原体、无乳链球菌感染的具体情况.结果:1206例患儿解脲支原体的总感染率、无乳链球菌的总感染率分别为12.02％和1.07％,男患儿与女患儿解脲支原体的感染率、无乳链球菌的感染率相比,差异无统计学意义(P>0.05).1206例患儿中男、女患儿上呼吸道解脲支原体感染的发生率及治愈率相比,差异无统计学意义(P>0.05).进行解脲支原体感染DNA检测的结果显示,145例解脲支原体感染患儿中男患儿与女患儿的低拷贝率、高拷贝率相比,差异无统计学意义(P>0.05);其中男、女患儿的低拷贝率均低于高拷贝率,差异有统计学意义(P<0.05).145例解脲支原体感染患儿中早产儿、新生儿败血症患儿、新生儿高胆红素血症患儿、高危儿和其他疾病患儿的占比相比,差异无统计学意义(P>0.05).13例无乳链球菌感染患儿中早产儿、新生儿败血症患儿、新生儿高胆红素血症患儿、高危儿和其他疾病患儿的占比相比,差异无统计学意义(P>0.05).结论:新生儿上呼吸道感染解脲支原体和无乳链球菌的传播途径主要是母婴传播,患儿感染解脲支原体后咽喉部所携带的病原体数量较多,且感染情况不受性别差异的影响.解脲支原体和无乳链球菌感染均可引起新生儿各系统的多种疾病,应引起临床重视,及时予以干预.

目的 筛选临床诊断为新生儿败血症的病原菌,以供临床诊断治疗.方法 采用高通量二代测序技术和传统细菌培养鉴定两种方法相结合,共同筛选47例临床诊断为新生儿败血症的病原菌,同时以15名正常新生儿作为对照组.使用生物信息学软件进行数据处理,采用秩和检验和x2检验进行组间差异分析.结果 高通量二代测序技术筛选临床诊断为新生儿败血症的病原菌阳性率为46.8％(22/47),而传统细菌培养鉴定阳性率为0.0％(0/47).早发败血症总阳性率为27.7％(13/47),晚发败血症总阳性率为19.1％ (9/47);临床诊断为新生儿败血症的主要病原菌为Geobacillus vulcani、Anoxybacillus kestanbolensis、产酸克雷伯菌和Acinetobacter guillouiae.结论 高通量二代测序技术筛选出临床诊断为新生儿败血症新的病原菌分子生物学指标,比传统细菌培养鉴定更适合于临床疾病诊治.

目的 探讨高通量二代测序技术在筛选感染性新生儿高胆红素血症病原菌中的应用价值.方法 运用高通量二代测序技术筛选22例感染性新生儿高胆红素血症患者的病原菌,同时进行传统细菌培养鉴定,比较二者的区别.结果 高通量二代测序技术检测病原菌阳性率为100.00％,传统细菌培养检测阳性率为0.00％.高通量二代测序技术筛选出的感染性新生儿高胆红素血症主要病原菌为Anox ybacilluskestanbolensis、Geobacillus vulcani、Klebsiella oxytoca和Acinetobacter guillouiae.结论 高通量二代测序技术具有高通量、高特异性、高准确度和快速等特点,适合临床患者病原菌的检测.

目的 探讨新生儿细菌性肺炎外周血自然杀伤细胞和淋巴细胞亚群与病程进展的关联.方法 选择2017年1月-2019年1月深圳市盐田区人民医院收治的细菌性肺炎新生儿80例为研究对象纳入观察组,另选同期健康体检新生儿50例为对照组.观察组患者、对照组入选者入院时分别采集空腹静脉血,用流式细胞仪检测自然杀伤细胞、淋巴细胞亚群等细胞水平,并进行组间比较.采用Pearson相关性检验对患儿的外周血自然杀伤细胞与淋巴细胞亚群的相关性进行分析探讨.结果 观察组患者的自然杀伤细胞包括CD1C、CD56+、CD59+等细胞水平均低于对照组,观察组内急性期患儿的CD16+、CD56+、CD59+细胞水平均低于恢复期患儿,三组间比较差异有统计学意义(P＜0.05).观察组患者的CD3+、CD4+、CD4+/CD8+水平低于对照组,CD8+水平高于对照组;观察组内急性期患儿的CD3+、CD4+、CD4+/CD8+水平低于恢复期患儿,CD8+水平高于恢复期患儿,三组间比较差异有统计学意义(P＜0.05).经Pearson相关性分析,CD16+、CD56+、CD59+与CD3+、CD4+呈正相关性,与CD8+呈负相关性.结论 细菌性肺炎新生儿体内的免疫系统紊乱,外周血自然杀伤细胞和淋巴细胞亚群的变化与疾病的进展及恢复有密切关联.因此,通过对细菌感染性肺炎患儿进行免疫功能的监测,对于疾病的进展、疗效评估有参考价值.

目的:探讨茵栀黄口服液联合间隙蓝光照射佐以双歧杆菌三联活菌治疗新生儿黄疸的临床疗效.方法:选取2014年1月～2017年1月在本院进行黄疸治疗的86例新生儿为主治对象,分为43例对照组与43例观察组.对照组采用常规方式进行治疗,给予观察组采用茵栀黄口服液联合间隙蓝光照射佐以双歧杆菌三联活菌治疗.待两组治疗后分别比较临床疗效、经皮胆红素水平、黄疸消退时间及不良反应发生情况.结果:观察组治疗总有效率高于对照组(97.67％VS76.74％),P<0.05;治疗后观察组经皮胆红素水平低于对照组(123.20±19.40μmol/LVS178.90±20.40μmol/L),P<0.05;黄疸消退时间短于对照组(2.30±0.60dVS5.60±0.80d),P<0.05;不良反应发生率低于对照组(9.30％VS30.23％),P<0.05.结论:对新生儿黄疸采用茵栀黄口服液联合间隙蓝光照射佐以双歧杆菌三联活菌治疗的临床疗效更为显著,可显著降低各项临床指标,并改善不良反应.

目的 探讨孟鲁司特钠辅助阿奇霉素治疗小儿喘息性支气管炎的效果.方法 选取2016年1月～2017年10月于我院就诊的120例患有喘息性支气管炎的患儿作为研究对象,依照随机数字表法将其分为单独用药组和联合用药组,每组各60例.单独用药组患儿给予阿奇霉素治疗,联合用药组患儿予以孟鲁司特钠联合阿奇霉素进行治疗.比较分析两组患儿用药后的治疗效果、症状改善时间、超敏C反应蛋白指标和不良反应发生率.结果 治疗后,联合用药组患儿的临床治疗总有效率(95.00％)高于单独用药组(78.33％),差异有统计学意义(P＜0.05).联合用药组患儿的咳嗽、喘息、哮鸣音改善时间,体温降至正常范围时间及住院天数均明显短于单独用药组,差异均有统计学意义(P＜0.05).治疗后,两组患儿的超敏C反应蛋白水平均低于治疗前,且联合用药组患儿的超敏C反应蛋白水平低于单独用药组,差异有统计学意义(P＜0.05).联合用药组患儿的不良反应发生率(3.33％)低于单独用药组(18.33％),差异有统计学意义(P＜0.05).结论 孟鲁司特钠对喘息性支气管炎的治疗有较好的辅助作用,能有效提高临床疗效,患儿的各种症状改善均较快,不良反应发生率低,具有安全、可靠的特点.

目的 探究分析在新生儿肺炎的治疗中运用丙种球蛋白静脉注射治疗的临床疗效以及对免疫功能的影响.方法 选取确诊为肺炎的新生儿80例,随机分为观察组(n=40)与对照组(n=40),对照组采用常规治疗方式;观察组在对照组的基础上给予丙种球蛋白治疗.观察两组的临床疗效及IgA、IgG、IgM免疫功能指标的变化情况.结果 经过一个疗程的治疗,观察组的总有效率为92.50％,显著高于对照组的75.00％ (P ＜0.05);治疗后观察组IgA、IgG、IgM均明显高于同时期的对照组(P＜0.05);治疗后观察组的临床指征均显著优于对照组(P＜0.05);两组不良反应发生率比较无统计学差异(P＞0.05).结论 静脉注射丙种球蛋白治疗新生儿肺炎临床疗效显著,可有效改善患儿的各项临床指征,缩短病程,促进新生儿体液免疫及细胞免疫功能的改善,同时引起的不良反应较少,值得临床应用推广.

目的 探讨阿莫西林克拉维酸钾和头孢他啶治疗新生儿肺炎的效果及其对肠道微生态的影响.方法 60例新生儿肺炎患儿,按照随机数字法分为对照1组、对照2组和研究组,各20例.对照1组予以阿莫西林克拉维酸钾治疗,对照2组予以头孢他啶治疗,研究组予以阿莫西林克拉维酸钾联合头孢他啶治疗.比较三组患儿临床疗效、治愈时间以及肠道各种益生菌数目.结果 研究组治愈率(80.00％)与对照1组(75.00％)、对照2组(85.00％)比较,差异无统计学意义(P>0.05).研究组治愈时间(5.32±1.31)d与对照1组(5.39±1.62)d、对照2组(5.42±1.37)d比较,差异无统计学意义(P>0.05).研究组肠道各种益生菌数目均显著低于对照1组、对照2组,差异有统计学意义(P<0.05).结论 阿莫西林克拉维酸钾和头孢他啶联合用药及单独用药治疗新生儿肺炎效果类似,但联合用药对肠道菌群影响更大.

Objective To observe and analyze the method and effect of using network platform in pediatric respiratory diseasetogivecontinuednursingintervention.Method 800casesofchildrenwithrespiratorydiseasehospitalizedinourhos‐pital were selected as study objects ,they were randomly divided into experimental group and control group by the digital method ,there were 400 cases of each group ,the control group adopted conventional discharge health guidance ,the experi‐mental group used the Internet platform for pediatric respiratory disease continue nursing ,after intervention of a year ,read‐mission rate 、medical cost 、satisfaction and the disease related knowledge awareness rate of family members within one year of the families of children of two groups were observed and compared .Results The disease related knowledge awareness rate increasing of family of children patients after one year of intervention was significantly higher than that of control group , comorbidity treatment and comorbidity cases hospitalized cases of intervention within 1 year were lower than those of the control group ,referral medical costs of the same disease within one year were lower than those of the control group ,nursing satisfaction of the experimental group was 98 .25% ,higher than 80 .00% of the control group ,the differences were statisti‐cally significant (P<0 .05) .ConclusionUsing network platform in pediatric respiratory disease used continue nursing can be helpful to improve the patients'knowledge of disease prevention ,and consolidate the family protection ability and health be‐havior ,reduce the referral rate ,improve the quality of life ,reduce medical costs ,improve medical efficiency ,guarantee chil‐dren's health ,and improve family happiness index .

Objective To study the changes of plasma tissue factor(TF) and tissue factor pathway inhibitor(TFPI) in newborn with asphyxia.Methods Sixty four newborn with asphyxia and thirty two normal newborn(control group)were selected.The levels of TF and TFPI in plasma of umbilical venous,asphyxiation extreme period and convalescent period were determined by using enzyme-linked immunosorbent assay(ELISA).Results Compared with control group,the plasma levels of TF in umbilical venous in newborn with asphyxia rose significantly(P0.05),while TFPI rose slightly(P0.05),TFPI/TF decreased a little(P0.05).In extreme period,TF levels of the two asphyxiated groups went on increased significantly,but TFPI and TF/TFPI decreased,they were both significantly compared with the control group(P0.01).The TF levels of severe asphyxia group was higher than that of slight asphyxia group(P0.05).In convalescent period,the TF levels of asphyxia groups came down obviously,there was no significant between the slight asphyxiated group and the control group(P0.05);But there was still significant between the severe asphyxia group and the control group(P0.05).The TP levels of the slight asphyxia group was lower than that of severe asphyxiated group,but there was no significant(P0.05).There were no significant between the levels of TFPI in the three groups(P0.05);The TFPI/TF of the asphyxia groups was lower than that in control group(P0.05),and the TFPI/TF of the slight asphyxia group was a little higher than that of sever asphyxiated group(P0.05).Conclusion Levels of TF and TFPI changed greatly in asphyxiated newborns,TF continual increased obviously,TFPI increased firstly,but decreased greatly as the hypoxia aggravated;The TFPI/TF decreased.All these changes were positively related with the degree of hypoxia.

目的观察新生儿缺氧缺血性脑病(HIE)心、肝、肾功能、消化道、及钙水平的变化状况。方法采用BECK-MAN-LX20全自动生化分析仪对HIE患儿及无窒息新生儿于生后72h内采血样的心肌酶谱,肝功能、尿素氮,肌酐,钙进行检测,检查大便常规及观察腹胀情况。结果HIE组患儿心肌酶,肝酶,血总胆红素水平均明显高于对照组(P<0.05)。HIE组血钙低于对照组(P<0.05),HIE组肾损害率高于对照组(P<0.05);HIE组消化道出血,腹胀情况明显高于对照组(P<0.05)。结论HIE同时可并发多器官功能损害,在治疗脑损伤的同时,应对其他各器官功能密切监测并加以保护.