为培养医学院学生动手能力和创新能力,训练学生的科研思维以及严谨的科研素养,血管紧张素转化酶基因多态性分析综合性实验被引进课堂.学生通过提取自己口腔粘膜上皮细胞基因组核酸、 经多聚酶链式反应扩增血管紧张素转化酶基因中具有多态性的片段、 凝胶电泳检测分析这一系列实验,得到自己的基因诊断结果的同时,全面、 深入、 系统地学习了这些常用技术的原理和方法,了解了其在临床研究中的应用,加深和巩固了学习的知识,激发了学生的学习兴趣和主动性,为将来的学习和科研打下良好的基础.

The Caenorhabditis elegans embryo is a widely used model for the functional analysis of dynamic cellular processes, such as chromosome segregation, cytokinesis or lineage analysis. However, the conventional embryo preparation method that relies on manually dissecting gravid worms to extract embryos is somewhat time consuming and does not lend itself to high throughput assays. Here, we report a fully integrated microfluidic approach for C. elegans early embryogenesis assays with unprecedented accuracy and throughput. The device consists of a compressible microfluidic pillar-array chamber for robust and fast on-chip extraction of embryos from the uterus of gravid nematodes. Subsequently, embryos are immobilized by automated fluidic transfer in a microtrap array for individual tracking of a large number of embryos, including fragile mutants with drug-permeable eggshells. Our device allows high-resolution live imaging of very early events in embryogenesis, starting from the one-cell stage. We also demonstrate the feasibility of well-controlled compound application in versatile microfluidic pharmacological assays performed on early embryos.

The nematode Caenorhabditis elegans is an important model organism for biomedical research and genetic studies relevant to human biology and disease. Such studies are often based on high-resolution imaging of dynamic biological processes in the worm body tissues, requiring well-immobilized and physiologically active animals in order to avoid movement-related artifacts and to obtain meaningful biological information. However, existing immobilization methods employ the application of either anesthetics or servere physical constraints, by using glue or specific microfluidic on-chip mechanical structures, which in some cases may strongly affect physiological processes of the animals. Here, we immobilize C. elegans nematodes by taking advantage of a biocompatible and temperature-responsive hydrogel-microbead matrix. Our gel-based immobilization technique does not require a specific chip design and enables fast and reversible immobilization, thereby allowing successive imaging of the same single worm or of small worm populations at all development stages for several days. We successfully demonstrated the applicability of this method in challenging worm imaging contexts, in particular by applying it for high-resolution confocal imaging of the mitochondrial morphology in worm body wall muscle cells and for the long-term quantification of number and size of specific protein aggregates in different C. elegans neurodegenerative disease models. Our approach was also suitable for immobilizing other small organisms, such as the larvae of the fruit fly Drosophila melanogaster and the unicellular parasite Trypanosoma brucei. We anticipate that this versatile technique will significantly simplify biological assay-based longitudinal studies and long-term observation of small model organisms.

The feasibility of time-controlled and reversible drug delivery to Caenorhabditis elegans (C. elegans) embryos immobilized on-chip is demonstrated by regulated blocking of cytokinesis using Cytochalasin-D. In article 1700751 by Martin A. M. Gijs and co-workers, the implementation of versatile microfluidic protocols, including time-controlled and reversible drug delivery to on-chip, immobilized embryos, demonstrates the potential of the reported device for biochemical and pharmacological assays.

The nematode Caenorhabditis elegans (C. elegans) is a powerful model organism for addressing fundamental biological questions related to human disease and aging. Accurate control of the number of worms is a primary step for quantitative biological or biochemical worm analysis and assays. However, conventional single-worm isolation methods often encounter operational complexity, involving manual manipulation steps that result in relatively low throughput and a lack of accuracy, with possible physical damage of the worms. Here, we propose an “8-channel Single Embryo Pipette” (8-SEP) that was specifically designed for rapid and highly efficient dispensing of single C. elegans embryos, from an embryo suspension directly into standard microtiter plates. Our microfluidic device takes advantage of pressure-tuned flexible embryo microtraps by means of which single embryos can be easily captured and released on-demand. 8-SEP fluidic manipulations do not have an adverse impact on embryo or worm physiology as monitored by normal development of the embryos after dispensing.

The genome of the nematode Caenorhabditis elegans shows far-reaching similarities to that of humans. C. elegans is therefore considered as a powerful model organism in biological research related to humans, for instance for the study of human diseases. Such studies are often based on high-resolution imaging of dynamic biological processes in the worm body tissues, requiring reliable immobilization of the nematodes. However, conventional immobilization methods may strongly affect physiological processes of the animal. Here, we report a new approach for on-chip immobilization of C. elegans nematodes, taking advantage of the elastic properties of polydimethylsiloxane (PDMS). We present two different microdevices, a micropillar array and a serpentine microchannel, respectively, both consisting of size-tunable PDMS structures that allow the same chips to be used for immobilization of worms at all development stages. Our microfluidic approach provides appropriate physiological conditions for long-term studies and enables worm recovery after the experiment. The performance of our devices is demonstrated by two different imaging experiments. The pillar array chip is used for in vivo live imaging of C. elegans embryo fertilization and early embryogenesis, whereas bacterial colonization of a worm's intestine was observed by means of the stretchable microchannel device.

从十堰国际金融中心的顶楼放眼望去,整个十堰市区尽收眼底.在这个市中心繁华地段,项目经理胡居宝对周边的地标性建筑如数家珍:人民广场、农行金穗大厦、建行金城大厦、十堰市图书馆、万德大酒店……一栋栋或宏伟或高峻的建筑环绕拱卫四周.这些建筑全部出自湖北工建三公司之手,如今就像巍峨矗立的丰碑,见证了湖北工建集团及其三公司的厚重历史;又如同陈列室里的奖杯,记录了湖北工建托起一座城市的光辉岁月.

The Caenorhabditis elegans embryo is a widely used model for the functional analysis of dynamic cellular processes, such as chromosome segregation, cytokinesis or lineage analysis. However, the conventional embryo preparation method that relies on manually dissecting gravid worms to extract embryos is somewhat time consuming and does not lend itself to high throughput assays. Here, we report a fully integrated microfluidic approach for C. elegans early embryogenesis assays with unprecedented accuracy and throughput. The device consists of a compressible microfluidic pillar-array chamber for robust and fast on-chip extraction of embryos from the uterus of gravid nematodes. Subsequently, embryos are immobilized by automated fluidic transfer in a microtrap array for individual tracking of a large number of embryos, including fragile mutants with drug-permeable eggshells. Our device allows high-resolution live imaging of very early events in embryogenesis, starting from the one-cell stage. We also demonstrate the feasibility of well-controlled compound application in versatile microfluidic pharmacological assays performed on early embryos.

Sentiment analysis strategy was used to predict stock market index.Support vector machine was applied to construct predict model based on textual information (i.e.,lexical information,sentimental words,and sentiment categories) extracted from social media and stock indicators.Experiment results show that the proposed method can obtain the best results,compared with many different predictive model.

Like other animals, C. elegans nematodes have the ability to socially interact and to communicate through exchange and sensing of small soluble signaling compounds that help them cope with complex environmental conditions. For the time being, worm biocommunication assays are being performed mainly on agar plates; however, microfluidic assays may provide significant advantages compared to traditional methods, such as control of signaling molecule concentrations and gradients or confinement of distinct worm populations in different microcompartments. Here, we propose a microfluidic device for studying signaling via diffusive secreted compounds between two specific C. elegans populations over prolonged durations. In particular, we designed a microfluidic assay to investigate the biological process of male-induced demise, i.e. lifespan shortening and accelerated age-related phenotype alterations, in C. elegans hermaphrodites in the presence of a physically separated male population. For this purpose, male and hermaphrodite worm populations were confined in adjacent microchambers on the chip, whereas molecules secreted by males could be exchanged between both populations by periodically activating the controlled fluidic transfer of μl-volume aliquots of male-conditioned medium. For male-conditioned hermaphrodites, we observed a reduction of 4 days in mean lifespan compared to the non-conditioned on-chip culture. We also observed an enhanced muscle decline, as expressed by a faster decrease in the thrashing frequency and the appearance of vacuolar-like structures indicative of accelerated aging. The chip was placed in an incubator at 20 °C for accurate control of the lifespan assay conditions. An on-demand bacteria feeding protocol was applied, and the worms were observed during long-term on-chip culture over the whole worm lifespan.

从3月3日全国政协开幕到3月16日的总理记者会，2016年的全国“两会”经过14天的紧凑日程圆满落下帷幕。本刊记者从全国“两会”新闻中心了解到，今年有3200多名中外记者报名采访“两会”。其中，境内记者有1900多人，港澳台记者有360多人，外国记者有1000多人。“两会”期间，共举办了15场记者会。本刊特派3名记者深入“两会”现场，从文化传媒角度为读者呈现记者眼中的“两会”特色。

Stock market prediction is always the hotspot of financial research. People usually use technique indicators to predicate the future value of stock index in the past. Recent years, people find that public emotion could also reflect the future changes of stock market and use it to predicate can bring us relatively ideal result. In this paper, we propose a new method that uses Support Vector Regression to construct predicate model, based on the combination of text information, containing words information, sentiment words information and sentiment classify results, extracted from sina microblogs and daily technique indicators. And those sentiment words are all coming from the sentiment lexicon and the public emotion is extracted from microblogs by two separate methods, sentiment lexicon and SVM. The experimental result shows that our method can provide us with an more ideal result. Keywords Stock market prediction; Sentiment analysis; Support vector regression.

文章通过对权威期刊《会计研究》载文数量、研究对象、基金项目、研究方法、作者分布等信息指标在演变过程中的特征分析，梳理和明确会计期刊未来发展趋向，这对于未来会计教学领域中学术理念的传播、会计无纸化的推广、会计职业道德和伦理的维护以及会计实务实训成果的转化都有理论和实践上的借鉴意义。

兴致勃勃从网上订购蔬果、海鲜等生鲜食品,收到后却发现冰袋已化,水果、海鲜坏了一大半.类似的情景很多消费者都遭遇过. 生鲜食品电商眼下是资本追逐的重点行业,以本来生活、天天果园、中粮我买网为代表的生鲜电商平台正逐步加大扩张步伐.然而网购生鲜逐渐成为一种消费趋势后,背后的冷链配送却成为最大的掣肘因素.

从正压防爆的基本原理出发,介绍了Bang-Bang控制在连续正压控制系统中的应用。

本刊讯(记者 李栋)12月17日下午,中央电视台七套《致富经》栏目举办的"农信银"杯2015 CCTV 十大三农创业致富榜样颁奖盛典,在中国农业电影电视中心演播大厅举行.评选推介出周素琴、邓洪九、潘东明、王伟伟、曾令钢、张杰、胡再明、占锦川、郑志军、金卫东等十位"2015CCTV十大三农创业致富榜样",周素琴、司全军、潘东明还分别荣获了"热心公益奖""执着精神奖""最佳商业模式奖"三个单项奖.

The roundworm Caenorhabditis elegans (C. elegans) is a powerful model organism for addressing fundamental biological questions related to human disease and aging. Its life cycle consists of an embryo stage, four larval stages that can be clearly distinguished by size and different morphological features, and adulthood. Many worm-based bio-assays require stage- or age-synchronized worm populations, for example for studying the life cycle and aging of worms under different pharmacological conditions or to avoid misinterpretation of results due to overlap of stage-specific response in general. Here, we present a new microfluidic approach for size-dependent sorting of C. elegans nematodes on-chip. We take advantage of the external pressure-deformable profile of polydimethylsiloxane (PDMS) transfer channels that connect two on-chip worm chambers. The pressure-controlled effective cross-section of these channels creates adjustable filter structures that can be easily tuned for a specific worm sorting experiment, without changing the design parameters of the device itself. By optimizing the control pressure settings, we can extract larvae of a specific development stage from a mixed worm culture with an efficiency close to 100% and with a throughput of up to 3.5 worms per second. Our approach also allows us to generate mixed populations of larvae of adjacent stages or to adjust their ratio directly in the microfluidic chamber. Moreover, using the same device, we demonstrated extraction of embryos from adult worm populations for subsequent culture of accurately age-synchronized nematode populations or embryo-based assays. Considering that our sorting device is merely based on geometrical parameters and operated by simple fluidic and pressure control, we believe that it has strong potential for use in advanced, automated, microfluidic C. elegans-based assay platforms.

旅游板块概念在上市公司中日益凸显为新兴的推动力量,前期大量研究为旅游上市公司的股权结构和价值管理提供了策略依据。在分析2010-2013期间旅游上市公司股权数据的基础上,将公司价值置于托宾Q这一视角中,对这一关系进行典型分析,提出了"托宾Q值——公司价值——股权治理"这一全新的研究思路,期待解释公司价值是否对股权治理对策产生影响进行实证检验,破解"公司价值——股权治理"过程的"黑箱",尝试得出旅游行业的特性在上市公司价值中的体现,对于优化股权结构提出一些建议,进而提高旅游企业整体价值会带来一些积极意义。