AIM: To synthesis and characterize a multi-functional siRNA delivery agent with effective thera-peutic effects and MR-tracing ability for programmed death ligand-1 ( PD-L1 ) positive gastric cancer SGC-7901 cell line . METHODS:The characterization , binding ability , cytotoxicity , transfection efficiency and cellular internalization of the polyplex were determined .The PD-L1 knockdown effect was analyzed , and cytokines secreted by cocultured T cells were measured.RESULTS:We developed folic acid (FA)-PEG-SS-PEI-SPION as siRNA delivery agent for PD-L1 knockdown. At N/P ratio of 10, the FA-PEG-SS-PEI-SPION bound PD-L1 siRNA to form polyplex in a diameter of (116.7 ±2.5) nm with zeta potential of (9.14 ±0.80) mV.Transfection efficiency of the targeted polyplex was (95.06 ±0.44)%, com-pared with ( 93.87 ±1.05 )% of the untargeted polyplex .Mean fluorescence intensity of the targeted polyplex was 1892.67 ±81.51, significantly higher than 1324.33 ±186.58 of the untargeted.The cellular magnetic resonance (MR) imaging showed the polyplex also acted as T 2 weighted contrast agent for cancer MR imaging .The relative mRNA level of PD-L1 in polymer/siRNA-2 treatment group was (9.07 ±0.79)%.Decreased protein expression of PD-L1 was showed by Western blot .The secretion levels of IFN-γand TNF-αin cocultured T cells increased , while that of IL-10 decreased . CONCLUSION:Our findings highlighted the potential of the multifunctional theranostic nanoparticles for effective targe -ting PD-L1 knockdown therapy and MR imaging diagnosis in gastric cancers .

Objective To explore the relation between the quality of life(QOL) and coping style of ovarian cancer patients under chemotherapy.Methods Totally,86 inpatients were investigated with the Functional Assessment of Cancer Therapy-Ovarian Cancer(FACT-O),the Medical Coping Modes Questionnaire(MCMQ).Results There was negative correlation between QOL and resignation coping(r=-0.535,P<0.01).Conclusion QOL is negative with resignation coping and better QOL could be achieved through the reducing of obedience coping.

To explore the problem of existence and countermeasures nurses in vacuum venous blood collection operation.Methods:Using stratified random sampling method,investigated the Institute 76 nurses,comparison of different degree,titles of nurses operating performance,qualified rate and the existing problems.Results:76 nurses vacuum venous blood collection for assessment scores(86.23± 7.75),a pass rate of 85.5%,the main problems with patient assessment,incomplete inspection project according to the condition of health education knowledge and inadequate.Conclusion:Advocating double check,strengthen the comprehensive evaluation of clinical nurses′ consciousness and ability,strengthen the key steps of implementation,and inspection related theoretical knowledge of the training,can improve the nurse vacuum venous blood collection operation nursing quality.

Objective To explore the relation between the quality of life(QOL) and coping style of ovarian cancer patients under chemotherapy.Methods Totally,86 inpatients were investigated with the Functional Assessment of Cancer Therapy-Ovarian Cancer(FACT-O),the Medical Coping Modes Questionnaire(MCMQ).Results There was negative correlation between QOL and resignation coping(r=-0.535,P0.01).Conclusion QOL is negative with resignation coping and better QOL could be achieved through the reducing of obedience coping.

Objective To explore the correlation between QOL and hope of ovarian cancer patients undergoing chemotherapy.Methods 86 inpatients were involved in the study.Data were gathered using the functional assessment of cancer therapy-ovarian cancer(FACT-O),the herth hope index scale(HHI).Results The QOL of ovarian cancer patients undergoing chemotherapy was relatively high with the total score of(97.29 ± 19.31).84.90% of them were in the median level and 15.10% in the high level.The hope of ovarian cancer patients undergoing chemotherapy was relatively high with the total score of(38.33 ± 4.46).70.90% of them maintained in high level and 29.10% in the median level.The QOL was positively correlated with hope in terms of physical well-being,social/family well-being,emotional well-being,life functional well-being,additional concerns subscales and total FACT-O.Conclusions Because hope is positively correlated with patients' QOL,nurses need to focus on performing psychosocial interventions to improve the hope.

<正>持续质量改进(continuous quality improvement,CQI)是一种促进产品质量达到更优、更高标准的有效方法,其基本观点是过程管理及改进,使产品更符合消费者的需要。它是新时期医院质量管理发展的重点[1]。

笔者在2006年12月-2007年8月赴香港进行了为期9个月的肿瘤专科护理的学习,接受香港大学专业进修学院和玛丽医院的共同培训,感受了不同文化背景下的医疗护理及管理特点,现报道如下。

目的:探讨妊娠合并卵巢良性肿瘤患者的手术前后主要护理问题并提出相应护理措施.方法:分析我院2001年1月~2005年5月31日间妊娠合并卵巢良性肿瘤手术病倒9例.结果:患者顺利度过手术,伤口恢复良好,B超和胎心监测显示胎儿生长良好.结论:通过术前术后评估病人,采取有效的护理措施,可以使妊娠合并卵巢良性肿瘤患者顺利度过手术关,继续安全妊娠.