新时代奋斗精神是对中国伟大革命实践中凝结形成的大无畏奋斗精神的继承和发扬,是对中国精神和中国力量的思考总结和凝练升华.新时代奋斗精神蕴含着丰富的内涵实质,集中体现为接力奋斗、共同奋斗、顽强奋斗、艰苦奋斗、永远奋斗,是人生观与价值观的统一、政治本色与政治责任的统一、伟大信仰和民族文化的统一、时代发展与时代需要的统一、时间维度与历史维度的统一.新时代践行伟大奋斗精神,需要我们瞄准宏伟目标、提振奋斗之志,不忘初心使命、凝聚奋斗之力,适应斗争形势、锤炼奋斗之能,与时间赛跑,珍惜奋斗之时.

思政教育作为社会与国家培养优秀人才的途径之一,是引导学生形成正确的三观,促使自身全面发展的关键,教师需要在实际教学中高度重视.爱国主义教育能够帮助学生全面、正确地了解中华民族的历史,激发其民族自豪感、历史荣辱感等,对做好思政教育有着非常深远的影响.因此教师在今后的教学中,要不断提高大学生思政课爱国主义教育实效性.

通过对当代大学生诚信缺失的主要表现、原因及诚信教育中存在问题进行解析,阐述了诚信班级体系构建的必要性,提出了诚信班级建设的管理构建模式.

新时代辅导员工作性质的特殊性、知识结构的多元化、成长成才的规律性对高校辅导员队伍建设提出更高要求.高校辅导员队伍当前存在的政治理论素养不高、工作实践能力不足、成长发展路径不宽、岗位任职定力不够等薄弱环节,要求高校辅导员队伍建设必须顺应新时代潮流,响应新时代号召,从主观和客观层面出发,在加强学习培养、优化知识结构、科学成长规划、完善制度保障上下功夫.

高校基层党组织是大学生骨干培养的重要抓手,在大学生思想政治教育中发挥着重要的作用.加强和提高基层党组织建设质量对于提升党员教育和凝聚吸引优秀青年意义重大.以强化学生政治素养,提升学生综合素质为目标,通过丰富党支部学习教育内容,创新教育载体,实施党员教育考核,选树学生身边先进典型,建立党员教育长效机制,全面提高大学生党支部组织建设质量,凝聚和吸引广大优秀青年,培养建设社会主义合格建设者和德智体美劳全面发展的人才.

Emphasis towards EQ cultivation enables postgraduates improve their ability of social contact,integrate themselves into the society more smoothly and realize their individual as well as social value more successfully.It's the vital approach for improving the postgraduates' EQ level that enhance the cognition for postgraduates themselves,tutors and the university,furthermore strengthen the cultivation about conception of senses of collectivity as well as responsibility.

The secondary management system of university is the inevitable trend of the development of higher education in our country.It is an important measure and realistic choice for further emancipating the mind,stimulating the vitality,realizing self-optimization,solving the drawbacks of the traditional internal management system and improving the quality of higher education teaching.In recent years,with the accelerated pace of education,the secondary management has been widely used.This paper analyzes forward the existing problems and coping strategies in the management of the current situation of the secondary management system to achieve the scientific,systematic,standardized and efficient management.

l -Phenylalanine is an important amino acid commercially, and therefore optimization of its manufacture is of interest. We constructed a range of mutant alleles of AroG, the enzyme involved in the first step of phenylalanine biosynthesis. Three single-site mutant alleles were constructed ( aroG8 , aroG15 , and aroG29 ), which were then combined to generate three double-site aroG fbr mutant alleles ( aroG8/15 , aroG8/29 , and aroG15/29 ). Enzymatic activity, feedback inhibition, and fermentation were analyzed in all of the mutants. All double-site mutants, except AroG15/29, showed higher enzymatic activity and greater resistance to feedback inhibition than their respective single-site mutants. The E. coli strain carrying the aroG8/15 allele produced a phenylalanine titer of 26.78 g/l, a 116 % improvement over the control phenylalanine overproducing strain (12.41 g/l). Our findings provide an effective method for modifying phenylalanine biosynthetic genes, which may be applied to optimize the commercial manufacture of phenylalanine.

Gene knockout has been broadly applied in genome reduction and metabolic engineering of Escherich ia coli.The scarless deletion technology based on homologous recombination provides clear application advantages over other knockout methods.This technology allows rapid and sequential deletion of targeted genes without intro duction of any exogenous sequences in the genome.In this article we presented the structures and functions of dif ferent types of vectors applied in scarless deletion,the principle and the procedure of scarless knockout technolo gy were also described in detail.