目的:通过模拟观察高压氧条件下种植体周围软组织愈合情况.方法:将12只比格犬的硬腭中份后份制备5mm的圆形组织缺损,随机分为AB两组各6只,A组给予高压氧治疗,B组不予处理.在第3天7天及14天术区取材对其进行愈合情况观察,组织学观察及CD31的免疫组化染色观察.结果:大体形态观察显示:A组创伤区逐渐愈合,14天上皮黏膜覆盖完全无组织凹陷及瘢痕的形成.B组术区愈合状态欠佳,14天时术区仍可见组织凹陷.苏木精-伊红染色(H-E染色)下观察到A组较B组在术后3天及7天时炎症细胞浸润更少.Mas-son染色后可见A组较B组在术后3天及7天时有更多的胶原纤维,更有序的排列.CD31免疫组织化学染色观察显示:A组较B组有更多的毛细血管生成,差异具有统计学意义.结论:高压氧治疗使得软组织愈合更快,愈合质量更佳,更减轻了术区的炎症反应.

Objective: The purpose of this study was to describe levels of IL-1β and TNF-α in gingival crevicular fluid of healthy peri-implant and teeth, and to provide a theoretical basis for maintaining the healthy of the peri-implants tissues. Methods:17 patients were enrolled in this study. Each patient has a healthy dental implant and a tooth(implant group:17 ITI implants, tooth group:17 teeth). The levels of IL-1β and TNF-α in gingival crevicular fluid of the peri-implants and teeth were analyzed quantita-tively by using enzyme linked immunosorbent assay and the correlation between level of the IL-1β and TNF-α in two groups was analyzed by using the Spearman. Results: the levels of IL-1β and TNF-αwere higher around implants than teeth. The levels of IL-1β in dental implants and teeth were respec-tively (Mean ±SD, 26.14 ±10.26; 21.93 ±6.43), but the difference of the levels of IL-1β between two groups was not statistically significants (P>0.05). The levels of TNF-α of implants and teeth were re-spectively(Mean±SD, 3.17±0.72;2.67±0.58), and the difference of levels of TNF-α between two groups was statistically significant (P<0.05). Significant positive correlation was noted between the IL-1β and TNF-α in implants group(P<0.05), and in teeth group(P<0.05). Conclusion: The levels of IL-1β and TNF-α in gingival creviscular fluid of healthy implants were higher compared with the natural teeth. The composition and structure of connective tissue around the implants, plaque and the implant itself, may make more production of the IL-1β and TNF-α in gingival creviscular fluid around the healthy implants than teeth.

目的 研究动态加载下不同骨缺损类型对种植体-骨整合界面、天然骨与Bio-oss成骨结合界面应力分布的影响.方法 应用ANSYS软件,建立第1磨牙缺失并伴有不同类型骨缺损(旁穿型、裂开型、环型、垂直型)的4种模型,并建立各模型对应的植骨块和种植体模型,以200 N垂直载荷及100 N斜向载荷分别模拟咀嚼周期中5个阶段的动态受力过程,分析各组织结合界面应力分布情况.结果 4类骨缺损模型在一个咀嚼周期中,从第2到第4阶段,种植体-骨整合界面应力增幅依次为81.6％、90.7％、106％、182％,Bio-oss成骨与天然骨结合界面应力增幅依次为26％、13％、6％、56％.结论 4类模型的植骨稳定性为:环型骨缺损＞裂开型骨缺损＞旁穿型骨缺损＞垂直型骨缺损,在临床上种植医师应根据其植骨部位甄别出植骨高风险患者,治疗时应为骨植入材料选择不同的固定方式,最终提高植骨稳定性;垂直载荷更有利于各组织结合界面的应力分布状况,在临床设计种植义齿上部结构时应尽量减小或避免斜向载荷.