The tumor metastasis suppressor gene CD82/KAI1 has been demonstrated to impact human trophoblast invasion and migration. Communication between trophoblasts and decidual stromal cells plays a crucial role in controlling the normal invasiveness of trophoblasts. However, whether CD82/KAI1 is involved in decidualization and what role it plays remain unclear. CD82/KAI1 demonstrates specific spatiotemporal expression patterns in stromal cells undergoing decidualization during pregnancy. This is observed in both naturally pregnant females post-implantation and pseudopregnant mice undergoing induced decidualization, as detected through in situ hybridization and immunofluorescence. CD82/KAI1 expression showed a significant time-dependent increase in cultured stromal cells after 24 and 48 h of progesterone (P4) and estrogen (E2) treatment. This was accompanied by a notable upregulation of decidualization markers, including cyclin D3 and PR. After transducing stromal cells with the adenovirus-overexpressing CD82/KAI1 for 48 h, the expression of cyclin D3 protein increased. Meanwhile, there was an attenuated expression of CD82/KAI1 due to an adenovirus siRNA knockdown, whereas cyclin D3 and PR expressions were not affected. Our findings suggest a potential role of CD82/KAI1 in regulating the process of decidualization, providing insights into stromal cell differentiation.

对某医科大学药学生进行调查研究,对不同家庭经济状况学生的家庭背景、消费水平、在校表现等进行比较研究.调查显示,农村户口或在农村成长、父母职业层级处于底层的学生中,家庭经济困难学生的比例更高;因病致贫是家庭经济困难的最主要原因;家庭经济困难学生在生活费和社交花费上明显低于非家庭经济困难学生;而在担任学生干部、获得奖学金、成绩排名以及发展规划等方面,不同家庭经济状况学生之间没有显著差异.总体而言,学生资助工作成效显著;建议加强精准资助力度,注重济困和育人相结合,进一步提升资助成效.

Objective Nuclear technology has been widely used in military and civilian fields, and radiotherapy is an effective and common form of treatment for cancer. However, acute radiation disease caused by high doses of radiation is a serious complication. The aim of this study was to investigate the chance of mitigating radiation-triggered hematopoiesis failure using human umbilical cord mesenchymal stem cell (HUCMSC) transplantation. Methods Umbilical cords were obtained from three full-term female neonatus through cesarean section at Xinqiao Hospital. Bone marrow mesenchymal stem cells (BMSCs) were cultivated as depicted before. Briefly, monocytes were collected from bone marrow blood by means of density separation columns. An acute radiation disease mouse model was established to compare the restoration effect of HUCMSCs and BMSCs transplanted via the tail vein. The hematopoietic stem cell transplantation (HSCT) mouse model was obtained through bone marrow cell transplantation (BMCT) from C57BL/6 mice (H-2b, donor) to female CB6F1 mice (H-2b×d, recipient) after irradiation. The mice were divided into five groups, including control (saline), irradiated (radiation), bone marrow (HSCT, transplanted 1×106 BM cells), HUCMSC (transplanted a mixture of 1×106 HUCMSCs and 1×106 BM cells), and BMSC group (transplanted a mixture of 1×106 BMSCs and 1×106 BM cells). The blood condition results were used to test the radiation-induced inflammatory reaction, and bone marrow pathological staining (H&E) was used to determine the radiation-induced bone marrow hematopoiesis failure. Results After radiation, HUCMSC transplantation significantly improved the survival rate. By analyzing the blood condition test, colony formation, and bone marrow pathology, it was found that the HUCMSC group demonstrated significant functional improvements in terms of the recovery from hematopoiesis failure and reduction of inflammatory reaction. Conclusions HUCMSCs have more advantages over BMSCs in restoring and promoting the recovery of radiation-induced hematopoietic damage, thus having a new therapeutic potential for patients with acute radiation disease.

γ‐Aminobutyrate (GABA) is commonly used as a food supplement and a health care product by young females, due to its positive roles in relieving stress, alleviating anxiety, and improving sleep. However, its recommended daily dose in different products varies widely. Besides, it is unknown whether, and how, GABA consumption during early pregnancy influences pregnancy establishment. In this study, we found that when pregnant mice were treated with a high (12.5 mg/g) dose of GABA (orally) during preimplantation, there was a reduction in the number of implantation sites on day 5 of pregnancy. Also, among these unimplanted embryos, most exhibited morphological degeneration and developmental retardation, and only a few of them developed into blastocysts but could not implant into the uterus. Moreover, the expression of uterine receptivity–related factors—LIF, E‐cadherin, and HOXA10—were all downregulated, while the number of uterine glands was reduced in the high GABA dose group. Finally, in vitro results demonstrated that GABA (ranging from 10 to 50 μg/μL) markedly inhibited preimplantation embryo development in a dose‐response manner. However, this inhibitory effect was not observed when the embryos were pretreated with 40 μΜ 2‐hydroxysaclofen, a GABAB antagonist, indicating that GABA exerts its inhibitory effects via its B‐type receptor. Our results suggest that exposure to certain GABA concentrations, during early pregnancy, can impair preimplantation embryo development via its B‐type receptor, and endometrial receptivity, which greatly disturbs early embryo implantation in mice. These findings could raise concerns about GABA consumption during the early stages of pregnancy.

通过对家庭经济困难大学生资助政策成效进行分析,对现行资助政策存在问题进行调研,就如何优化现行资助体系提出合理化建议,以提升学生资助工作成效.研究表明,现行资助政策能有效解决家庭经济困难学生的经济问题,在促进高等教育机会平等和助力精准扶贫上发挥了积极的作用;同时也存在资助经费渠道单一、资助类型分布不合理、资助着力点单一等不足.基于上述分析,建议在稳步加大多渠道资助经费投入的基础上,坚持育人导向,更加注重人文关怀,构建经济资助、精神激励、人文关怀和能力提升相互融合的资助体系,以最终形成“解困-育人-成才-回馈”的良性循环.

目的 研究富硒米糠代替有机硒添加对仔兔生长性能和抗氧化功能的影响.方法 将60只35日龄断奶仔兔随机分为普通米糠组、有机硒组、富硒米糠组,每组20只,雌雄各半,分别饲喂普通米糠饲料、普通米糠加有机硒饲料、富硒米糠饲料,共饲喂30 d,记录每日饲料消耗量.分别于实验第0、5、10、15、20、25、30天称量实验兔体重,计算每组实验兔料重比.饲喂结束后,采集各组实验兔血清,检测血糖、尿酸、总蛋白、白蛋白、球蛋白含量、白蛋白/球蛋白比及血清谷胱甘肽过氧化物酶(GSH-Px)活性、超氧化物歧化酶活性(SOD)、过氧化氢酶(CAT)活性和丙二醛(MDA)含量;采集各组实验兔肝、脾、肾并称重,计算各组组内脏器指数,并检测肾硒含量.结果 有机硒组和富硒米糠组的日增重、料重比、脏器指数、肾硒含量以及上述血液生化指标同普通米糠组相比,差异均无显著性(P>0.05),但富硒米糠组的GSH-Px和SOD活性、有机硒组的GSH-Px和CAT活性较普通米糠组均有显著增加(P<0.05),两组的MDA含量较普通米糠组均有明显降低(P<0.05),且富硒米糠组和有机硒组的抗氧化能力相当.结论 富硒米糠对仔兔生长性能和抗氧化功能的促进作用与有机硒相当,可代替有机硒作为仔兔饲料硒的来源.

目的 建立C57BL/6小鼠抑郁模型,初步探究莫扎特K448奏鸣曲中的高频段声波改善C57BL/6小鼠抑郁症状的效果.方法 1)慢性应激模型的建立:小鼠依据自主活动实验结果剔除活动次数差异较大者,其余分为空白组(n=10)、模型组(n=36),模型组经历5周慢性温和不可预知刺激(chronic unpredictable and mild stress,CUMS),建立小鼠抑郁模型.(2)治疗干预:造模成功后,将模型组小鼠随机均衡分为模型对照组(n=12)、氟西汀组(n=12)和音乐组(n=12).氟西汀组每天腹腔注射盐酸氟西汀溶液(10 mg/kg),其余两组注射等量的生理盐水.音乐组每天进行2 h高频音乐干预,其余两组不进行音乐干预.干预持续2周.(3)效果评价:实验前3 d及实验中每周称量体重并记录,实验第1周、第5周、第7周进行悬尾实验(tail suspension test,TST)和强迫游泳实验(forced swimming test,FST).第7周行为学实验结束后,取小鼠脑组织制备匀浆,通过酶联免疫吸附法(enzyme-linked immunosorbent assay,ELISA)测定脑源性神经营养因子(brain derived neurotrophic factor,BDNF)含量.结果 1)成功构建CUMS小鼠模型.第5周模型组小鼠悬尾不动时间明显增加,差异有显著性(P<0.01),强迫游泳不动时间增加,差异有显著性(P<0.05).(2)氟西汀组与模型对照组相比,悬尾实验不动时间明显缩短,差异有显著性(P<0.01),强迫游泳实验不动时间缩短,差异无显著性(P>0.05);音乐组与模型对照组相比,悬尾不动时间缩短,差异有显著性(P<0.05),强迫游泳实验不动时间无明显改变,差异无显著性(P>0.05).模型对照组与空白组小鼠相比,脑组织匀浆中的BDNF含量明显降低,差异有显著性(P<0.01);氟西汀组与模型对照组相比,脑组织匀浆中的BDNF含量明显回升,差异有显著性(P<0.01),但音乐组与模型对照组相比,其差异无显著性(P>0.05).结论 莫扎特K448奏鸣曲高频段声波可一定程度优化小鼠抑郁模型的治疗作用.

Placenta plays multi-functions in embryo-uterine dialogue through facilitating gas and nutrient exchange, providing an immunological barrier between the fetus and mother and secreting hormones and growth factors to regulate pregnancy. The successful formation and development of placenta requires invasion and differentiation of trophoblast cells, and any defects would result pregnancy related diseases such as intrauterine growth retardation (IUGR), preeclampsia (PE). Lat1 (L-type amino acids transporter 1) is a major Na+ independent transporter of large neutral amino acids, including several essential amino acids. It has been showed that amino acid was fundamental regulator on cell function and energy metabolism in early embryonic development. It has been reported that Lat1 mRNA expressed in zygote, blastocyst during the pre-implantation stages and trophoblast giant cells (TGCs) in post-implantation placenta in mouse. Little is known the role of lat1 on placentation. Our research was to explore the effects of lat1 on the placentation in mouse. The expression of lat1 was detected from day 9 to 18 of pregnancy in placenta. The effects of lat1 on placentation were assessed with inhibitor of leucine transport 2-aminobicyclo-(2, 2, 1)-haptane-2-carboxylic acid (BCH) treatment by uterine horns injection on day 8 (D8) of pregnancy. The protein of lat1 was mainly localized in the cytoplasm of maternal decidual cell, spongiotro-phoblast cell (Sp) and labyrinth (Lab). Inhibition of lat1 transportation activity by uterine horns injection with BCH in vivo results in disorder of placental anatomical structure in mid-late pregnancy. These results suggest that lat1 might play an important role in mouse placentation progress.

We previously found that cullin 4A (CUL4A) is able to promote trophoblast invasion. However, the role of CUL4A in other trophoblast behavior such as migration and the association with pregnancy disorder remains unclear. In this study, Immunostaining revealed that CUL4A was relatively lowly expressed in placentas from pre-eclampsia patients compared with the normal controls. Spiral arteries and inadequate trophoblast migration remodeling result in poor placental perfusion, which mediated maternal injury and may lead to pre-eclampsia. To confirm CUL4A's function on trophoblast migration, we employed human villous explants culture and trophoblast cell line HTR-8/SVneo migration and invasion assay. According to our data, knocking-down CUL4A expression inhibited villous explant's outgrowth significantly in vitro, and down-regulated migration of HTR8/SVneo cells (P<0.01). This effect might be due to reduced matrix metalloproteinases activities, whereas the apoptosis and proliferation of trophoblast cells were not affected. Moreover, CUL4A siRNA increased the levels of both tissue inhibitors of MMPs (TIMP)-1 and -2 significantly. These results suggested that CUL4A may play an essential role in trophoblast cells' migration and dysregulation of CUL4A may be associated with pre-eclampsia.

Objective:To investigate the effects and possible mechanisms of γ-aminobutyric acid (GABA) on preimplantation embryo development in mice.Methods:①The embryos at different development stages were collected and the localization of GABA,B-type receptor protein (GB 1),the π subunit of A-type receptor (GABRP),and L-glutamic acid decarboxylase 67 (GAD67) was detected by immunofluorescence staining.②The embryos at different development stages were cultured in vitro in KSOM medium containing 10,20,30,40,or 50 μg/μL GABA and 40 μmol/L 2-hydroxysaclofen(GABA B-type receptor antagonist) for 48-72 h,respectively,and blastocyst formation rate was counted.Results:①GABA and GB1 are mainly expressed in the membranes of 2-cell and 4-cell embryos,while in the 8-cell to blastocyst stages,they are localized in the nucleus.However,GABRP was not detected in preimplantation embryos at any stage.In addition,GAD67,a key GABA synthetase,was mainly localized in the cytoplasm in preimplantation embryos.②The blastocyst formation rate of each development stage was significantly declined in a dose-dependent manner.In addition,the blastocyst formation rate of 2-cell embryos stage was remarkably inhibited by 10 μg/μL GABA (92％ vs.99％,x2=5.70,P=0.035).③ 40 μmol/L 2-hydroxysaclofen could effectively reverse the inhibitory effect of 30 μg/μL and 40 μg/μL GABA on blastocyst formation (99％ vs.45％,x2=72.32,P=0.000;99％ vs.4％,x2=180.66,P=0.000).Conclusion:GABA could affect the development of preimplantation embryos through its B-type receptor in mice.