To report a case of staged surgical excision of giant porokeratosis.A 54-year-old man had been seeking medical attention for 29 years due to an itchy purplish-red spot on the right upper abdomen.Dermatological examination revealed a well-defined round purplish-red patch,ap-proximately 15 cm x 15 cm,with rough keratinization and few attached scales on the surface.Brown color was observed around the lesion without any noticeable elevation,erosion or exudation.Histopathological analysis showed hyperkeratosis and parakeratosis,loss of granular layers,irregu-lar thickening of the stratum spinosum,superficial dermal vasodilation and perivascular infiltration of mononucleated cells along with melanophages.Dermoscopy revealed dark red background with clear boundaries,whitish peripheral rim and linear vessels,focal hypopigmentation,comma-like vessels at center,as well as thickened yellow scales in the surrounding area.Diagnosis was poro-keratosis of Mibelli.The skin lesion was surgically removed using a staged excision method over four stages,resulting in successful healing without recurrence during one-year follow-up.

Objective To investigate the characteristics of lesions and clinical features of ke-loids.Methods This study included a total of 395 patients with keloids who were diagnosed and treated at the Dermatology Department of the Second Hospital of Hebei Medical University between October 2022 and October 2023.Patients'general information,characteristics of onset and treat-ment history were analyzed.Results The male-to-female ratio was 1∶1.04.The peak onset age was between 19～28 years old.The chest(48.35％)and shoulders/back(22.28％)were the two most commonly affected areas.One hundred sixty-two(41.01％)patients had single lesion on single site.Patients with a family history of keloids accounted for 4.30％.Among the 395 pa-tients,74.94％of the patients had not received any treatments,while 25.06％had a history of treatment prior to visiting our clinic.Twelve patients had received multiple or combination modali-ty treatments,and 87 patients had received single modality treatment.Patients who had received previous treatments experienced recurrence.Conclusions Keloids primarily affect young individ-uals,predominantly on the chest and shoulders/back,and mostly present as single lesion on one body site.There are no significant differences in onset sites,clinical classification and lesion sizes between genders.Genetic factors can affect the development of keloids.Patients with keloids are often treated with local drug injection,surgical resection,and other methods.But the recurrence rate is high,indicating a challenge in the treatment of keloids.

目的 基于Web of Science核心合集数据库收录的文献,通过文献计量方法分析尖锐湿疣(CA)的研究现状及未来发展趋势.方法 检索2005～2022年Web of Science数据库核心合集中收录的尖锐湿疣相关文献,运用文献计量学方法,使用VOSviewer和CiteSpace软件进行可视化分析,将结果以可视化形式呈现.结果 共检索到2249篇尖锐湿疣相关文献,美国在出版物总数、总引用次数和H指数方面排名第一,发文量最多的研究机构是美国默克集团,刊文量最多的期刊是Vaccine,发文量最多的作者是Suzanne Garland.尖锐湿疣的研究热点已从发病机制、疫苗转向光动力治疗.结论 应用VOSviewer和CiteSpace软件进行可视化分析,可反映近20年尖锐湿疣相关文献的数量变化及分布情况.目前光动力疗法是尖锐湿疣研究领域的研究热点.

目的 基于Web of Science核心合集数据库收录的文献,通过文献计量方法分析玫瑰痤疮的研究现状及未来发展趋势.方法 检索 2005 至 2022 年Web of Science数据库核心合集中收录的玫瑰痤疮相关文献,运用文献计量学方法,使用VOSviewer和CiteSpace软件进行可视化分析,将结果以可视化形式呈现.结果 共检索到 2 075 篇玫瑰痤疮相关文献,这些文献来自于 82 个国家的 2 508 个机构的 7 750 名作者,发表在 511 种期刊上.美国在出版物总数、总被引频次和H指数方面排名第一.发文量最多的机构是加利福尼亚大学.刊文量最多的期刊是《Journal of Drugs in Dermatology》.发文量最多的作者是James Del Rosso,H指数最高的作者是Richard L.Gallo.通过CiteSpace突现词分析可总结出目前该领域的研究热点为玫瑰痤疮共病及疾病负担.结论 应用VOSviewer和CiteSpace软件进行可视化分析,可客观反映出 2005 至 2022 年玫瑰痤疮文献数量变化及分布情况,玫瑰痤疮的研究热点已从其病因及治疗逐渐转移到共病及疾病负担等方面.

Objective:To investigate efficacy and advantages of combined local flaps in repairing large defects in patients with nasal and perinasal non-melanoma skin cancers after Mohs micrographic surgery.Methods:From March 2018 to November 2020, 11 patients with nasal and perinasal non-melanoma skin cancers, who underwent Mohs micrographic surgery followed by repair with combined local flaps, were collected from Department of Dermatology, the Second Hospital of Hebei Medical University. According to the location and size of postoperative defects, flaps were designed based on the nasal aesthetic subunit principle. For large defects that could not be directly sutured or covered by a single local flap, 2 or 3 kinds of flaps were applied in combination, such as kite flap, modified diamond flap, nasolabial skin flap, bilobed skin flap, etc.Results:Among the 11 patients, 10 were diagnosed with basal cell carcinoma and 1 with squamous cell carcinoma, and the area of defects ranged from 2.0 cm × 2.3 cm to 2.7 cm × 3.6 cm. After Mohs micrographic surgery combined with local skin flap repair, all skin flaps survived well without blood supply obstruction, the texture, color and contour of the skin flaps were similar to those of the surrounding normal skin, and no obvious scars were formed. During the postoperative follow-up of 4 to 32 months, no recurrence of the tumors occurred, and the patients was satisfied with the appearance.Conclusion:To repair large defects using combined skin flaps of 2 or 3 kinds after Mohs micrographic surgery in patients with nasal and perinasal non-melanoma skin cancers can maintain the normal nasal or perinasal morphological structure and aesthetic appearance, and yield a satisfactory cosmetic effect.

<span id="ChDivSummary" name="ChDivSummary" class="abstract-text">目的探讨Kv7通道开放剂氟吡汀、瑞替加滨与阿司匹林联合应用的镇痛作用,为临床联合用药提供科学依据。方法实验分为对照组、阿司匹林组、氟吡汀组、瑞替加滨组、阿司匹林+瑞替加滨组、阿司匹林+氟吡汀组。采用冰醋酸致扭体法、热板法评价各组药物的镇痛效果。结果扭体法中,与阿司匹林组相比,阿司匹林+氟吡汀组的扭体次数明显减少（P&lt;0.01）,阿司匹林+瑞替加滨组的扭体反应潜伏期明显延长（P&lt;0.01）,扭体次数明显减少（P&lt;0.01）;热板法中,与阿司匹林组相比,阿司匹林+氟吡汀组与阿司匹林+瑞替加滨组的舔后足潜伏期延长率均增加,其中,阿司匹林+瑞替加滨组在30 min、60 min时的舔后足潜伏期延长率与阿司匹林组相比有明显差异（P&lt;0.05）。结论 Kv7通道开放剂氟吡汀、瑞替加滨可以增强阿司匹林的镇痛作用。</span>

Aim:To determine copy number of the enterovirus 71( EV71) in clinical specimens by quantitative analysis , and compare 3 commercial TaqMan fluorescence quantitative PCR methods systematically and comprehensively .Methods:VP1 sequence of EV71 was inserted into cloning vector pMAL-c2X.The purified plasmid was used as the standard sample for quantitative detection of 105 clinical specimens collected from the special monitoring system of HFMD in Henan Province . Positive rate and CT value among the 3 commercial TaqMan fluorescence quantitative PCR methods from bioPerfectus Technol-ogies(method A), KINGHAWK(method B), and Beijing ABT(method C) were compared, and consistency was analyzed . Results:The recombinant plasmid pMAL-c2X was constructed successfully .Standard curve equation was Y=-3.35 X+47.49.For 105 specimens, positive rate of EV71 measured by method A, B, and C was 41.90%(44/105), 42.86%(45/105), and 41.90%(44/105), respectively, and the difference was not significant (P=0.987).Among positive specimens, copy number by method C(14.17 ±3.19) was less than method A(24.34 ±3.92) and B(25.08 ±3.94), and the difference was significant(P<0.05).For 3 methods, the limit of detection was 102, 10, and 102 copies; sensitivity was 93.62%, 95.74%, and 93.62%;specificity was 100.00%, 75.00%, and 100.00%.The result of 3 methods showed poor consisten-cy.Conclusion:EV71 fluorescence quantitative PCR kit for commercialization is suitable for the qualitative detection of clini -cal samples;however , it needs further study for the quantitative detection of EV 71.

Objective To evaluate different detection methods in the diagnosis of severe fever with thrombocytopenia syndrome (SFTS), and find the most quick and accurate one for the identification of new bunyavirus infection. Methods Real-time PCR and ELISA-IgM were used to detect serum samples of 158 patients with acute phase of SFTS, which were collected from the special monitoring system of SFTS in Henan Province in 2014. IgM and IgG antibodies were detected by ELISA in 109 acute and convalescent paired serum specimens. The differences of the positive rates were compared between the three methods, and the influence of the collected interval time on the detection results was analyzed. Results For 158 acute phase serum samples of SFTS patients, the positive rate detected by real-time PCR (76.58%) was higher than that of ELISA-IgM (47.47%), and the difference was statistically significant (χ2=34.13, P < 0.05). For 109 cases with acute and convalescent paired serum samples, there was no significant difference in the positive rates between ELISA-IgG ( 75.23%) and real-time PCR (72.48%) detections (χ2=0.18, P>0.05). In both the acute phase and convalescent phase, the positive rate of IgM was higher than that of IgG, and the difference was statistically significant (χ2=41.68 and 6.25, P<0.05). With the extension of collected interral time, the positive rates of IgM and IgG antibodies were both increased ( Z=6.42 and 10.08, P < 0.05). Conclusion Real-time PCR is the most sensitive method for the early diagnosis of the SFTS. ELISA-IgG is suitable for the detection of SFTS at recovery period. ELISA-IgM can be used as an assistant method to guide clinical diagnosis.