BACKGROUND Bilateral multilobular hepatocellular carcinoma (HCC) complicated by HCC-derived biliary tumor thrombus is classified as an advanced-stage disease. Owing to the heavy tumor burden, obstructive jaundice, and other issues, the prognosis with conventional treatment is extremely poor, and most patients lose the opportunity for radical resection. The advent of immune-combined targeted conversion therapy has brought new hope for such patients. CASE SUMMARY A 36-year-old man with >= 10-year history of hepatitis B was admitted for upper abdominal distension and pain. Imaging demonstrated bilateral multifocal HCC with a left HCC-derived biliary tumor thrombus (Chinese Liver Cancer stage IIIa, Barcelona Clinic Liver Cancer stage C), obstructive jaundice, and cirrhosis. The tumor was initially considered unresectable. The patient received conversion therapy with camrelizumab, apatinib mesylate, and radiofrequency ablation. After five cycles, both the tumors and thrombus regressed, and tumor marker levels decreased markedly. The response was assessed as partial response according to mRECIST 1.1 criteria. Liver function improved from Child-Pugh class B to class A, allowing radical surgical resection with negative margins. Postoperative maintenance therapy was administered for 1 year. No recurrence was detected during follow-up. CONCLUSION For advanced bilateral multi-lobular HCC complicated by HCC-derived biliary tumor thrombus, immune-targeted therapy combined with local ablation reduces tumor burden, eliminates thrombus, converts unresectable disease to resectable status, and achieves effective short-term disease control.

患者 男性，49岁，主因“右上腹间断隐痛2个月余”于2020年2月28日收入我院。2个月前患者无明显诱因出现右上腹间断隐痛，无放射痛。自行口服“消炎利胆片”，其间症状加重，至当地医院行上腹增强CT检查，结果提示肝实性占位，考虑肝癌；实验室检查诊断乙肝，予口服恩替卡韦片（0.5 mg/d）抗病毒治疗。为进一步治疗就诊于我院。既往史无特殊。母亲曾患肝硬化，因“肝病”去世。入院后体检未见明显阳性体征。实验室检查：甲胎蛋白>1 210.00 μg/L，癌胚抗原及CA19-9均在正常范围，乙肝表面抗原、表面e抗体、核心抗体阳性，乙肝病毒DNA定量检测549 IU/ml。肝功能Child-Pugh分级为A级。入院行腹部增强MRI检查结果提示肝右叶占位，大小约10.3 cm×10.7 cm×14.3 cm，动脉期不均匀强化，静脉期及延迟期强化减退，腔静脉、肝右静脉、肝门静脉右支及主干多发栓子形成；肝硬化、脾大（图1）。根据《原发性肝癌诊疗指南（2019年版）》，初步诊断为：（1）原发性肝癌，中国肝癌分期Ⅲa期；巴塞罗那肝癌临床分期为C期；（2）慢性乙肝；（3）肝硬化；（4）脾大、脾功能亢进。

The present study aimed to explore the role of histone chaperone anti-silencing function 1B (ASF1B) in pancreatic cancer and the underlying mechanism. The biological function of ASF1B was investigated in pancreatic cancer cell lines (PANC-1 and SW1990) and a mouse xenograft model. Chromatin immunoprecipitation was used to detect the effect of ASF1B on the transcriptional activity of c-Myc. ASF1B was highly expressed in pancreatic adenocarcinoma (PAAD) samples from The Cancer Genome Atlas. ASF1B expression was positively associated with poor survival rates in patients with PAAD. Silencing of ASF1B in PANC-1 and SW1990 cells inhibited cell proliferation, migration and invasion, and induced apoptosis. Mechanistically, ASF1B increased H3K56 acetylation (H3K56ac) in a CREB-binding protein (CBP)-dependent manner. ASF1B promoted H3K56ac at the c-Myc promoter and increased c-Myc expression. In PANC-1 and SW1990 cells, the CBP inhibitor curcumin and the c-Myc inhibitor 10058-F4 reversed the promoting effects of ASF1B on cell proliferation, migration and invasion. In the mouse xenograft model, ASF1B silencing inhibited tumor growth, and was associated with low H3K56ac and c-Myc expression. ASF1B promoted pancreatic cancer progression by activating c-Myc via CBP-mediated H3K56ac.

High hepatitis B virus (HBV) DNA level is an independent risk factor for postoperative HBV-associated liver cancer recurrence. We sought to examine whether HBV DNA level and antiviral therapy are associated with survival outcomes in patients with advanced hepatocellular carcinoma (HCC) treated with anti-programmed cell death protein 1 (PD-1)based immunotherapy. This single-institution retrospective analysis included 217 patients with advanced HBV-related HCC treated from 1 June 2018, through 30 December 2020. Baseline information was compared between patients with low and high HBV DNA levels. Overall survival (OS) and progression-free survival (PFS) were compared, and univariate and multivariate analyses were applied to identify potential risk factors for oncologic outcomes. The 217 patients included in the analysis had a median survival time of 20.6 months. Of these HBV-associated HCC patients, 165 had known baseline HBV DNA levels. Baseline HBV DNA level was not significantly associated with OS (P = 0.59) or PFS (P = 0.098). Compared to patients who did not receive antiviral therapy, patients who received antiviral therapy had significantly better OS (20.6 vs 11.1 months, P = 0.020), regardless of HBV DNA levels. Moreover, antiviral status (adjusted HR = 0.24, 95

Nanoparticles possess the ability to adsorb and load other compounds. This study aimed to synthesize a gene carrier with polyethyleneimine (PEI), hyaluronic acid (HA) and mesoporous silica nanoparticles (MSNs) for circ_0086375 delivery to investigate the role and mechanism of circ_0086375 in pancreatic cancer (PC) progression. The expression of genes and proteins was detected by quantitative real-time polymerase chain reaction and Western blot. In vitro experiments were performed by cell counting Kit-8 (CCK-8), 5-Ethynyl-2′-deoxyuridine (EdU) assay, flow cytometry, transwell assay, and wound healing assay, respectively. Dual-luciferase activity assay was used to investigate the target relationship between miR-646 and circ_0086375 or SLC4A4 (solute carrier family 4 member 4). Circ_0086375 loaded PEI/HA-based mesoporous silica nanoparticles (MSNs) were prepared, and in vivo assay was performed by using xenograft tumor model. Circ_0086375 expression was decreased in PC tissues and cells. Restoration of circ_0086375 suppressed PC cell proliferation, migration and invasion in vitro and in vivo. Mechanistically, circ_0086375 acted as a sponge for miR-646 to elevate SLC4A4 expression, which was confirmed to be a target of miR-646. The prepared circ_0086375/MSN/PEI/HA nanocomplexes showed excellent fluorescent properties and a higher cellular uptake of circ_0086375 in PC cells. Moreover, circ_0086375/MSN/PEI/HA showed relatively more anticancer effects in PC than that of circ_0086375 alone in vitro and in vivo. Delivery of circ_0086375 by nanoparticles suppresses the tumorigenicity of pancreatic cancer by miR-646/SLC4A4 axis, suggesting a new potential target for future pancreatic cancer treatment.

In recent years, silver nanoparticles (AgNPs) have been used as key chemotherapeutic drugs to treat various cancers like prostate, breast, ovarian, and blood cancers. No previous reports demonstrated the in vitro anti-human pancreatic cancer properties of the novel chemotherapeutic drug formulated by silver nanoparticle compounds including Zingiber officinale leaf. To survey the anti-human pancreatic cancer activities of AgNO3, Zingiber officinale leaf aqueous extract, and silver nanoparticles, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay was used on common human pancreatic cancer cell lines. According to the Field Emission Scanning Electron Microscopes (FE-SEM) and Transmission electron microscopy (TEM) images, the silver nanoparticles were in an average size of 18.93 nm with a spherical shape. 2,2-diphenyl-1-picrylhydrazyl (DPPH) test revealed similar antioxidant potentials for Zingiber officinale leaf aqueous extract, silver nanoparticles, and butylated hydroxytoluene. Silver nanoparticles inhibited half of the DPPH molecules in the concentration of 172 µg/mL. Silver nanoparticles had very low cell viability and anti-human pancreatic cancer properties dose-dependently against AsPC-1, PANC-1, and MIA PaCa-2. The IC50 values of the silver nanoparticles were 295, 312, and 220 µg/mL against AsPC-1, PANC-1, and MIA PaCa-2 cell lines, respectively. It is thought that the silver nanoparticles obtained can be used as an anticancer drug for the diagnosis of pancreatic cancer in humans after acceptance of the above findings in clinical study trials.

Objective: To investigate the effects of lncRNA LINC00839 on the proliferation, migration and invasion of hepatocellular carcinoma cells and its mechanism. Methods: Real-time quantitative polymerase chain reaction (RT-qPCR) was used to detect the expression of LINC00839 and miR-3666 in hepatocellular carcinoma tissues and adjacent tissues. Pearson correlation was used to analyze the correlation between LINC00839 and miR-3666 expression in liver cancer tissues. Hepatocellular carcinoma cells MHCC97H were cultured in vitro and divided into si-NC group, si-LINC00839 group, miR-NC group, miR-3666 group, si-LINC00839+ anti-miR-NC group, and si-LINC00839+ anti-miR-3666 group. Methylthiazoletrazolium (MTT) method and clone formation experiment were used to detect cell proliferation. Transwell array was used to detect the cell migration and invasion. Western blot was used to detect the protein expressions of p21, E-cadherin and MMP-2. The double luciferase reporter gene experiment was used to verify the regulatory relationship between LINC00839 and miR-3666. Results: Compared with adjacent tissues, the expression level of LINC00839 in hepatocellular carcinoma tissues increased (2.82±0.27 vs. 0.96±0.10, P<0.001), but the expression level of miR-3666 decreased (0.23±0.02 vs. 1.01±0.10, P<0.001). The expression levels of LINC00839 and miR-3666 in liver cancer tissue were negatively correlated (r=-0.658, P<0.001). The survival rate of MHCC97H cells in the si-LINC00839 group [(53.91±5.41)% vs. (100.53±10.22)%], the number of clones formed (92.0±8.0 vs. 164.0±14.3), the number of migration (131.0±12.7 vs. 247.0±22.4), the number of invasion (66.0±6.4 vs. 120.0±11.6) and the protein level of MMP-2 (0.20±0.02 vs. 0.67±0.06) were lower than those in the si-NC group (P<0.001). However, the protein levels of p21 (0.76±0.07 vs. 0.25±0.02) and E-cadherin (0.78±0.08 vs. 0.14±0.01) were higher than those in the si-NC group (P<0.001). LINC00839 targeted and negatively regulated the expression of miR-3666. The survival rate of MHCC97-H cells in the miR-3666 group [(47.93±4.86)% vs. (100.11±10.21)%], the number of clone formation (78.0±7.7 vs. 166.0±15.9), the number of migration (117.0±12.1 vs. 250.0±25.0), the number of invasion (57.0±5.7 vs. 121.0±12.3) and the protein level of MMP-2 (0.16±0.01 vs. 0.69±0.07) were lower than those in the miR-NC group (all P<0.001). However, the protein levels of p21 (0.83±0.08 vs. 0.24±0.02) and E-cadherin (0.87±0.09 vs. 0.13±0.01)were higher than those in the miR-NC group (all P<0.001). The survival rate of MHCC97-H cells in the si-LINC00839+ anti-miR-3666 group [(89.94±9.05)% vs. (54.12±5.39)%], the number of clones (143.0±13.8 vs. 94.0±9.4), the number of migration (208.0±19.8 vs. 129.0±12.6), the number of invasion (108.0±10.1 vs. 65.0±6.4) and the protein level of MMP-2 (0.31±0.03 vs 0.66±0.06) were higher than those in the si-LINC00839+ anti-miR-NC group (P<0.001). However, the protein levels of p21 (0.31±0.03 vs. 0.74±0.07) and E-cadherin (0.28±0.03 vs. 0.80±0.08) were lower than those int the si-LINC00839+ anti-miR-NC group (P<0.001). Conclusion: Inhibition of LINC00839 expression may inhibit the proliferation, migration and invasion of hepatocellular carcinoma cells by targeting up-regulation of miR-3666 expression.

骨尤文肉瘤是一种多见于儿童及青少年的原发性骨肿瘤.骨外尤文肉瘤/外周原始神经外胚层肿瘤较为少见,多位于深部软组织,其中位于十二指肠更为罕见,治疗方式多以手术为主,现报道1例患者的治疗经过,供临床参考.

Gallbladder cancer (GBC) is one of the leading causes of cancer-related mortality worldwide. Researchers have investigated that specific strains of bacteria are connected with growth of different types of cancers in human. Some reports show possible implication of Helicobacter pylori (H. pylori) in the etiology of gallbladder cancer (GBC). Their enigmatic mechanisms, nevertheless, are not still well clear. We sought to predict whether various proteins of H. pylori targeted to nucleus of host cells and their implication in growth of gallbladder cancer. GBC is one of the leading causes of cancer mortality worldwide. We applied bioinformatics approach to analyze the H. pylori proteins targeting into the nucleus of host cells using different bioinformatics predictors including nuclear localization signal (NLS) mapper Balanced Subcellular Localization (BaCelLo) and Hum-mPLoc 2.0. Various nuclear targeting proteins may have a potential role in GBC etiology during intracellular infection. We identified 46 H. pylori proteins targeted into nucleus of host cell through bioinformatics tools. These H. pylori nucleus-targeting proteins might alter the normal function of host cells by disturbing the different pathways including replication, transcription, translation etc. Various nucleus-targeted proteins can affect the normal growth and development of infected cells. We propose that H. pylori proteins targeting into the nucleus of host cells regulate GBC growth using different strategies. These integrative bioinformatics research demonstrated several H. pylori proteins that may serve as possible targets or biomarkers for early cure and treatment or diagnosis GBC.

病人,女,29岁,已婚.因上腹疼痛不适15天,发现肝占位7天于2019年2月12日入院.15天前无明显诱因出现上腹疼痛不适,伴有纳差、乏力,行彩超检查提示:肝内多发稍高回声团.随后行上腹部平扫MRI检查提示:肝内多发占位,考虑不典型肝细胞肝癌可能性大;肝右叶胆管细胞癌并肝内转移,转移瘤暂不除外.既往体健,无吸烟、饮酒史,否认结核、疟疾等传染病病史,无既往手术史,无输血、献血史,乙肝、丙肝等病毒性肝炎病史不详.

Pancreatic cancer is a malignant tumor of the digestive system with occult onset, low early diagnosis rate, rapid progression and poor prognosis. Perineural invasion (PNI) of pancreatic cancer is considered as an important factor which leads to the poor prognosis of pancreatic cancer. Neural invasion models of pancreatic cancer can simulate the occurrence and development of neural invasion of pancreatic cancer under the complex tumor microenvironment, which is an important carrier to study the molecular mechanism, early diagnosis and treatment improvement of PNI of pancreatic cancer. The current PNI model could be broadly divided into in vivo model and in vitro model, and in vivo model could further be divided into ectopic model and in situ model. Recently, based on the in vivo and in vitro models, genetically engineered mouse models and patient-derived xenograft models have been applied. Here, the preparation methods, clinical uses and limitations of the commonly used neural invasion in vitro and in vivo models of pancreatic cancer are reviewed.

The study analysed healthcare workers' (HCWs) knowledge, practices, and attitudes regarding coronavirus disease 2019 (COVID-19). A cross-sectional survey was conducted from February 4th to February 8th, 2020, involving a total of 1357 HCWs across 10 hospitals in Henan, China. Of those surveyed, 89% of HCWs had sufficient knowledge of COVID-19, more than 85% feared self-infection with the virus, and 89.7% followed correct practices regarding COVID-19. In addition to knowledge level, some risk factors including work experience and job category influenced HCWs' attitudes and practice concerning COVID-19. Measures must be taken to protect HCWs from risks linked to job category, work experience, working hours, educational attainment, and frontline HCWs.

目的 探讨原发性肝癌(PLC)患者肝癌非根治性切除术中肝断面处理方法对术后复发的影响.方法选择2013年1月至2015年12月郑州大学附属肿瘤医院收治的139例PLC患者为研究对象,所有患者行肝癌非根治性切除术,根据术中肝断面处理方法分为观察组和对照组.对照组38例患者术中肝断面只做结扎止血处理;观察组101例患者术中肝断面分别给予高频电刀烧灼(28例)、放置植入用缓释氟尿嘧啶(49例)、放置125 I放射性粒子(24例)处理.分别于治疗前、治疗后检测患者血清丙氨酸转氨酶(ALT)、谷草转氨酶(AST)、尿素氮及全血白细胞(WBC)水平,观察2组患者术后引流量、住院时间及胆漏、腹腔出血、腹腔感染发生率;所有患者术后随访1 a,统计术后6个月、6～12个月内肿瘤复发率.结果2组患者手术前后血清ALT、AST、尿素氮及WBC水平比较差异均无统计学意义(P﹥0.05).观察组患者术后胆漏、腹腔出血及腹腔感染发生率分别为6.93％(7/101)、2.97％(3/101)、14.85％(15/101),对照组患者术后胆漏、腹腔出血及腹腔感染发生率分别为5.26％(2/38)、2.63％(1/38)、13.16％(5/38);2组患者术后胆漏、腹腔出血及腹腔感染发生率比较差异均无统计学意义(χ2=0.126、0.011、0.064,P﹥0.05).2组患者术后引流量及住院时间比较差异均无统计学意义(t=1.440、0.521,P﹥0.05).观察组患者术后6个月、6～12个月肿瘤复发率分别为12.87％(13/101)、8.04％(7/87),对照组患者术后6个月、6～12个月肿瘤复发率分别为28.95％(11/38)、11.11％(3/27);观察组患者术后6个月肿瘤复发率显著低于对照组(χ2=4.995,P﹤0.05),但2组患者术后6～12个月肿瘤复发率比较差异无统计学意义(χ2=0.242,P﹤0.05).观察组患者中,术中肝断面高频电刀烧灼患者术后6个月、6～12个月肿瘤复发率分别为14.29％ ％(4/28)、12.50％(3/24),肝断面放置植入用缓释氟尿嘧啶患者术后6个月、6～12个月肿瘤复发率分别为12.24％(6/49)、6.98％(3/43),肝断面放置125 I放射性粒子患者术后6个月、6～12个月肿瘤复发率分别为12.50％(3/24)、4.76％(1/21);肝断面3种处理方法患者的术后6个月、6～12个月肿瘤复发率比较差异无统计学意义(χ2=0.965、1.026,P﹤0.05).结论肝癌切除术中因肿瘤较大、紧邻重要管系或肝功能较差等原因无足够的切缘时,肝断面应该进行必要的处理,肝断面高频电刀烧灼、放置植入用缓释氟尿嘧啶、放置125 I放射性粒子可以明显降低肝癌术后短期复发率.

Objective To investigate the expression of miRNA-182 in hepatocellular carcinoma（HCC） cell lines and tissues,to verify the targeted regulation relationship between miRNA-182 and forkhead box transcription factor 2（FOXF2） and to explore whether miRNA-182 can promote the invasion,metastasis and angiogenesis of HCC

目的:观察雷公藤甲素对人胰腺癌荷瘤裸鼠的抑瘤作用,探讨其作用的分子机制.方法:选择健康BALB/c裸鼠于右后肢皮肤移植人胰腺癌SW1990细胞,建立裸鼠荷瘤模型,将建模成功的裸鼠分为模型组、吉西他滨组和雷公藤甲素组,每组10只,另选10只健康裸鼠作为对照组.测量各组SW1990胰腺癌荷瘤裸鼠体质量,取肿瘤组织称质量,计算肿瘤抑制率;采用免疫组织化学法检测各组裸鼠胰腺组织中凋亡相关蛋白Bax和Bcl-2的表达情况;采用Western blotting法检测各组裸鼠胰腺组织中凋亡相关蛋白Bax、Bcl-2、Caspase-9和Caspase-3表达水平.结果:与吉西他滨组比较,雷公藤甲素组SW1990胰腺癌的肿瘤抑制率明显升高(P＜0.05).HE染色观察,与模型组比较,雷公藤甲素组裸鼠胰腺癌肿瘤细胞增殖受到抑制.免疫组织化学检测,与模型组比较,雷公藤甲素组裸鼠胰腺组织中Bcl-2表达水平和Bcl-2/Bax比值明显降低(P＜0.05).Western blotting法检测,与模型组比较,雷公藤甲素组裸鼠胰腺组织中Bax、Caspase-9和Caspase-3蛋白表达水平均明显升高(P＜0.05),而Bcl-2蛋白表达水平明显降低(P＜0.05).结论:雷公藤甲素能抑制裸鼠肿瘤组织的生长,其机制可能与抑制胰腺肿瘤组织中Bcl-2的激活、促进Bax的表达、降低Bcl-2/Bax的比值、促使Caspase-9和Caspase-3激活及诱导胰腺癌移植瘤细胞凋亡有关.

BACKGROUND:Pancreatic cancer (PC) is one of the deadliest cancers about the digestive system. Recent researches have validated that long non-coding RNAs (lncRNAs) play vital roles in various cancers, while the function of LINC01006 in PC is rarely clarified.AIM OF THE STUDY:Investigation of the specific role of LINC01006 in PC.METHODS:LINC01006 expression was examined by RT-qPCR. CCK-8, EdU, transwell, wound healing, and western blot assays were carried out to explore the function of LINC01006 in PC. The interaction among LINC01006, miR-2682-5p and HOXB8 was verified by luciferase reporter, RIP and ChIP assays.RESULTS:The expression of LINC01006 was markedly upregulated in PC tissues and cells. Furthermore, LINC01006 knockdown inhibited PC cell proliferation, invasion and migration, and upregulation of LINC01006 led to the opposite results. Besides, miR-2682-5p expression was downregulated and negatively regulated by LINC01006 in PC. Meanwhile, LINC01006 could bind with miR-2682-5p in PC. Moreover, miR-2682-5p negatively regulated HOXB8 expression and there was a binding site between miR-2682-5p and HOXB8 in PC. Additionally, miR-2682-5p overexpression or HOXB8 knockdown rescued the promotive effects of LINC01006 upregulation on PC cell progression. Similarly, miR-2682-5p inhibition or HOXB8 overexpression countervailed the repressive role of LINC01006 downregulation in PC cell progression. In addition, the transcription factor HOXB8 could activate LINC01006 transcription in PC.CONCLUSIONS:LINC01006 promotes cell proliferation and metastasis in pancreatic cancer via miR-2682-5p/HOXB8 axis, which may facilitate the treatment for PC.

Objective To select the best preoperative biliary drainage (PBD) method for patients with hilar cholangiocarcinoma. Methods The PubMed, EMBASE, Web of Science, CNKI, and Wanfang Database were systematically searched for prospective or retrospective studies on biliary drainage for patients with hilar cholangiocarcinoma with obstructive jaundice. The drainage-related cholangitis, pancreatitis, hemorrhage, and the success rate in relieving jaundice were analyzed. The meta-analysis was performed using the Review Manager 5.3 and the stata 12.0 using a fixed or random effects model. Results This meta-analysis included 12 studies with 1567 patients. The results showed a lower risk of cholangitis with PTBD than EBD (RR=0.60, 95%CI: 0.39~0.95, P<0.05). PTBD also resulted in a lower risk of pancreatitis than EBD (RR=0.30, 95%CI: 0.15~0.59, P<0.05), and a higher rate of successful relief of cholestatic jaundice (RR=2.77, 95%CI: 1.79~4.28, P<0.05). However, the risk of bleeding for PTBD was higher (RR=2.38, 95% CI: 1.12~5.05, P<0.05), the risk of intraoperative blood transfusion increased (RR=1.59, 95% CI: 1.05-2.42, P<0.05), and the risk of celiac metastasis was also increased (RR=3.24, 95%CI: 1.15~9.12, P<0.05) when compared with EBD. The incidence of celiac metastasis was as high as 4.2%. There were no significant differences between PTBD and EBD in the rates of bile leakage, intra-abdominal abscesses, hemorrhage, R0 resection, postoperative hospital stay postoperative complications and in-hospital mortality, what's more, there were no significant differences in the incidence of cholangitis, pancreatitis, and liver abscess between ENBD and EBS. Conclusions The postoperative hospital stay was similar between the two groups. ENBD was a better choice than PTBD for patients who required PBD. PTBD could be used after the failure of ENBD. Key words: Endoscopic biliary drainage; Percutaneous transhepatic biliary drainage; Hilar cholangiocarcinoma; Klatskin tumor; Meta-analysis

探讨黏蛋白(MUC)、表皮生长因子受体(EGFR)在胆囊结石和胆囊息肉患者胆囊组织中的表达及意义.选取2015年2月—2017年12月收治的胆囊结石患者56例(胆囊结石组),胆囊息肉患者40例(胆囊息肉组),同时选取正常胆囊标本50例作为对照组,采用半定量PCR检测各组胆囊组织MUC和EGFR mRNA的相对表达.胆囊结石组MUC1、MUC2、MUC5AC和MUC5B mRNA相对表达量分别为80.02±12.03、70.27±11.37、110.02±23.29和167.20±22.11,明显高于胆囊息肉组和对照组(P<0.05);胆囊结石组MUC3 mRNA相对表达为20.04±9.11,明显低于胆囊息肉组(P<0.05),而与对照组比较差异无统计学意义(P>0.05);胆囊结石组MUC6 mRNA相对表达为60.04±17.17,明显高于对照组(P<0.05),而与胆囊息肉组比较差异无统计学意义(P>0.05);胆囊结石组EGFR mRNA相对表达为48.10±11.26,明显高于胆囊息肉组和对照组(P<0.05);胆囊结石患者EGFR mRNA与MUC5AC mRNA相对表达呈正相关(r=0.562,P<0.05),与MUC1、MUC2、MUC3、MUC5B和MUC6 mRNA相对表达无相关性(P>0.05).MUC和EGFR基因可能与胆囊结石的发生有一定关系,其中MUC5AC与EGFR存在一定的相关性.