The clinical data of 1 patient with pulmonary alveolar proteinosis were collected and the related literatures were reviewed. Patient was hospitalized with air shortness of time for 4 months, 1-month exacerbation, and did transbronchial biopsy. After Periodic acid-Schiff staining positive, the patient was diagnosed as pulmonary alveolar protein deposition. After the whole lung lavage for 2 times, the patient was given subcutaneous injection of granulocyte-macrophage colony stimulating factor after the disease repeating; the condition of the state improved as well. It suggests that subcutaneous injection of granulocyte-macrophage colony stimulating factor in idiopathic PAP patients is a viable method.

目的：探讨盐酸氯丙嗪联合斑蝥酸钠维生素 B6对人喉癌 Hep－2细胞株的增殖抑制作用及其对细胞周期进程的影响。方法应用四甲基偶氮唑蓝（MTT）比色法检测单独斑蝥酸钠维生素 B6制剂及盐酸氯丙嗪联合斑蝥酸钠维生素 B6制剂对 Hep－2细胞的增殖抑制率，流式细胞术检测细胞周期进程的变化。结果单独斑蝥酸钠维生素 B6组对 Hep－2细胞增殖有明显的抑制作用，而斑蝥酸钠维生素 B6联合氯丙嗪效果明显高于单独斑蝥酸钠维生素 B6组。流式细胞仪结果显示，G1期细胞增多，S 期细胞减少，G2／M 期细胞相对增多，且细胞凋亡率升高。结论斑蝥酸钠维生素 B6对 Hep－2细胞生长、增殖及其对细胞周期进程均有抑制作用，尤其是氯丙嗪联合斑蝥酸钠维生素 B6组效果显著。

目的：检测二肽基肽酶（ DPP）Ⅱ在不同病程的老年性白内障患者晶状体中的表达及酶活性变化，探讨DPPⅡ与老年性白内障发病的关系。方法晶状体混浊程度不同的老年性白内障患者根据晶状体核硬度不同进行分组。 Western印迹法检测 DPPⅡ在各组晶状体中的表达变化，应用蛋白酶活性测定DPPⅡ紫外吸光度，计算DPPⅡ在各组晶状体中的酶活性变化。结果随着老年性白内障患者晶状体核硬度的增加，DPPⅡ的表达增加，同时，DPPⅡ的酶活性增强。结论 DPPⅡ随晶状体核硬度增加而呈现表达及酶活性的变化与老年性白内障进展有关。

目的研究苏木乙醇提取物(SME)对坐骨神经损伤小鼠免疫功能的影响。方法选取成年雄性Balb/c小鼠60只,随机分为SME高中低剂量组和空白对照组,行右侧坐骨神经离断吻合术,分别于术后3、5、7、14、28d以MTT法测定淋巴细胞增殖转化能力,ELISA法测定血清循环免疫复合物(CIC)浓度。结果各时间点小鼠淋巴细胞增殖转化能力及血清CIC浓度与SME剂量呈明确的量效关系。结论SME能够影响坐骨神经损伤小鼠的细胞及体液免疫功能,且与用药剂量存在明确量效关系。

Objective To investigate the immune expression of dipeptidyl peptidase Ⅲ(DPP Ⅲ) in the each tissues of normal rat eyeball.Methods The normal Wistar rats of both sexes were chosen,the immune expression of DPP Ⅲ in the each tissues of normal rat eyeball were investigated with ABC immunohistochemical method.Results DPP Ⅲ were all found positively by immunohistochemical method in corneal epithelial cells,corneal endothelial cells,ciliary body non-pigment epithelial cells,iris pigment epithelial cells,lens epithelial cells,lens fibroblasts,retinal nerve fiber layer,retinal ganglial cells,retinal outer plexiform layer,retinal pigment epithelial cells.Conclusion DPP Ⅲ are primarily in the corneal epithelial cells,corneal endothelial cells,ciliary body non-pigment epithelial cells,iris pigment epithelial cells,lens epithelial cells,lens fibroblasts,retinal nerve fiber layer,retinal ganglion cells,retinal outer plexiform layer,retinal pigment epithelial cells of Wistar rats.

Background It has been determined that dipeptidyl peptidase Ⅲ (DPPⅢ) plays an important role in the metabolism and modification of proteins and DPPⅢ of human has highly homologous to rat.Researches have shown that DPPⅢ is associated with the formation of cataract.However,few relevant studies have been reported.ObjectiveThe present study is to find out the relationship between the expression of DPPⅢ in rat lenses and age-related cataract.Methods Lenses were obtained from general Wistar rats of ages 3,6,9,or 12 weeks old (10 lenses each ) and homogenized with different concentrations of standard bovine serum.The proteins were resolved using SDS-PAGE gel electrophoresis.Peak concentration and total concentration of DPPⅢ protein in lenses from rats of different ages were detected by Western blot.Enzyme activity of DPPⅢ was determined by monitoring the amount of dipeptides removed from a special substrate (Arg-Arg-4mb NA) by measuring the absorbance with UV-2500PC at 525 nm.The relationship between the enzyme activity of DPPⅢ in lenses and age of rats was evaluated using regression analysis.Results DPPⅢ was detected at a molecular weight of 82000 Da.The peak concentration and total concentration of DPPⅢ in normal rat lenses increased with the growth of age.The total protease activity of DPPⅢ in the lenses of rats was correlated with the ages of the rats (r=0.99,P＜0.05).Conclusion DPPⅢ may be involved in the alteration of crystallin during the development of lenses,and it may play an important role in the formation and aggravation of age-related cataract. Key words: dipeptidyl peptidase Ⅲ; lens; age-related cataract; enzymes activity

Objective To explore the effect of Caesalpinia sappan ethanol extract on the myelin basic protein in blood serum and the nerve function in mouse after sciatic nerve injury.Methods Adult male Balb/c mouse were randomly divided into the normal control-group and the experimental-group with high middle or low dose of Caesalpinia sappan ethanol extract,the right sciatic nerve was transected and inosculated.The mouse survived for 3,5days and 1,2,4,8,12weeks after operation.The MBP was detected by ELISA and the nerve function was detected by electromyography.Results The concentration of MBP in blood serum and the resules of electromyography had dose-effect relationship with SME.Conclusion Caesalpinia sappan ethanol extract could effect the regeneration of nerve in mouse after sciatic nerve injury,there is dose-effect relationship between SME and the effection.

AIM:To investigate the immune expression of dipeptidyl peptidase II(DPPII)in the normal eyeball tissues.· METHODS:The eyes were removed after the rats were sacrificed and postfixed with 40g/L formalin for 20 hours,dehydrated in ethanol,substituted with bezene,and embedded in paraffin wax.Paraffin sections about 4μm thick were cut by using a microtome and mounted on slides.Then the immune expression of DPPII in the normal eyeball tissues were investigated with ABC method.· RESULTS:DPPII found in corneal epithelial cells,corneal stroma,corneal endothelial cells,ciliary non-pigment epithelial cells,iris pigment epithelial cells,lens epithelial cells,lens fiber,retinal nerve fiber layer,ganglion cells,retinal pigment epithelium cells showed positive staining.· CONCLUSION:DPPII are primarily in the corneal epithelium cells,corneal stroma layer,corneal endothelial cells,ciliary non-pigment epithelial cells,iris pigment epithelial cells,lens epithelial cells,lens fiber,retinal nerve fiber layer,ganglion cells,retinal pigment epithelium cells.

目的:研究二肽基肽酶II（dipeptidyl peptidase II,DPPII）在D-半乳糖性白内障大鼠晶状体和正常大鼠晶状体中含量的变化,探讨DPPII与白内障发病的关系。方法:建立D-半乳糖性白内障大鼠动物模型,进行相应分期分组,应用Western blot方法检测DPPII在各组白内障大鼠及正常大鼠晶状体中含量变化。结果:DPPII在各组D-半乳糖性白内障大鼠晶状体中的含量明显高于同期正常对照组,并且随着晶状体混浊程度的加重而增加。结论:DPPII在D-半乳糖性白内障大鼠晶状体中的含量增加,可能与白内障发病相关。

• Aim: To compare and to clarify the relation between dipeptidyl peptidase II (DPPII) and cataractogenesis by comparing the changes of protein level of DPPII in the lens of established D-galactose-induced cataractous rat models and normal rats. • Methods: D-galactose-induced cataractous animal models were established in rats, and then the models and normal rats were divided into different groups with different development stages. Western blot was used to detect the changes of protein level of DPPII in the lens of each group of cataractous rats and the normal. • Results: The protein level of DPPII in the lens of rats of cataractous group was significantly higher than that in the control group, and increased with the development of the lens opacification in the cataractous group. • Conclusion: The changes of protein level of DPPII may play a role during cataract formation.

Objective To explore the relationship between dipeptidyl peptidaseⅡ(DPPⅡ) and the pathogenesis of congenital cataract.Methods Eighty SCR rats with congenital cataract were randomly divided into four groups:8,10,12 and 14 weeks groups(n=20).Normal Wistar rats at each age were used as control groups.Immunohistochemical experiments using the streptavidin-biotin immunoper-oxidase method were used to observe the immunoreactivity changes of DPPⅡ in lens in SCR rats at different time(8,10,12,14 weeks).Results The immunoreactivities of DPPⅡ in the lens epithelial cells and fibres of cataractous lenses of all ages were higher than those in control groups.At the 12th and 14th week,immunoreactive staining of DPPⅡwas found to extend into the perinuclear region.Conclusion The immunoreactivity of DPPⅡ is enhanced in cataractous rat lenses.DPPⅡ may be participated in the proteolytic modification of lens proteins during cataractogenesis.

Objective To investigate changes of the immunoreactivity of αB-crystallin in normal rat lens and congenital cataractous shumiya rat lenses,and to explore the relationships between the changes and the pathogenesis of cataract.Methods Eyes from cataractous and normal shumiya rats were conducted immunohistochemical experiments in the 8th,10th,12th and 14th week after birth,respectively,using the streptavidin-biotin immunoper oxidase method to observe the changes of immunoreactivity.Results An intense αB-crystallin reactivity was detected in epithelium and fibres in the lens of all control rats at any ages.The reactivity of αB-crystallin in lens fibres of shumiya rats remained intense until the 10th week,and was reduced on both the 12th and 14th week.Conclusion The reactivity of αB-crystallin is obviously reduced in the lens fibres in shumiya rats on the 12th and 14th week,indicating that αB-crystallin is essentially degraded by some peptidases during cataract formation.

目的观察8、10、12、14周龄正常及先天性白内障大鼠晶状体中dipeptidyl peptidase Ⅲ(DPP Ⅲ) 的免疫活性变化,探讨其与白内障发病的关系.方法分别于8、10、12、14周龄鼠, 摘取正常对照组以及先天性白内障大鼠晶状体, 应用抗生物素蛋白-生物素-过氧化物酶复合体法, 观察不同时期大鼠晶状体中DPP Ⅲ的免疫活性变化.结果各周龄先天性白内障大鼠晶状体纤维部DPP Ⅲ的免疫活性明显高于正常对照组, 特别是12、14周白内障晶状体中DPP Ⅲ的免疫阳性产物扩展至晶状体核周部.结论 DPP Ⅲ在白内障大鼠晶状体中免疫活性增强, 它有可能在白内障形成过程中参与晶状体蛋白的水解分化, 从而促进白内障的形成.

为研究低钙动物的生长代谢及与疾病的关系.介绍一种可行的不同血钙水平大鼠动物模型,通过控制膳食内钙水平对所饲大鼠血钙进行调节.用Wistar大鼠,按体重和性别均匀分层分为7组.所用的基础LC饲料,是以玉米面为主的膳食配方所配成,其中低钙特点突出,所饲大鼠血钙水平明显低下,体重生长迟缓、代谢偏低.研究结果表明,LC组大鼠血清Ca水平仅及Stock组一半,体重增长迟缓,当膳食补Ca后,血Ca水平随补Ca量逐渐升高,而体重增长也随之变化升高,呈明显的量效关系,LC+600Ca组血Ca接近正常水平.当膳食内总钙在1000mg/kg时,即LC+600mg/kg组,上述生长迟缓的状态即被改善,P＜0.01.