A chromatography–spectrophotometry method is developed for determining total ecdysteroids in terms of 20-hydroxyecdysone in Serratula centauroides (L.) and Fornicium unflorum (L.) raw materials, based on the purification of extracts from the plant raw materials with aluminum oxide and subsequent spectrophotometric determination relative to a 20-hydroxyecdysone reference sample. Ecdysteroids were determined in plants by HPLC; rhapontisterone and turkesterone are found in F. uniflorum for the first time.
We determined promising sources of phytoecdysteroids - Rhaponticum uniflorum (L.) and Serratula centauroides (L.), growing in the Buryat Republic. Biologically active substances were founded: ecdysteroids, phenolic compounds, polysaccharides, flavonoids, tannins, saponins, vitamins.