Biological effects of nitric oxide are multiply, including vasoactive activity, participation in neurotransmission and intercellular communication etc. These effects are associated with endogenous releasing of nitric oxide, but influence of exogenous administration of this substance does not study in details. In particular, systemic action of nitric oxide inhalations is not so clear. The aim of this work was the investigation of nitric oxide inhalations action on some parameters of energy and oxidative metabolism of healthy rat blood. Wistar rats were randomly divided into two groups: control group (without any manipulations; n=10) and main group (n=10) with inhalations by nitric oxide-containing gas flow (20 ppm). Lactate dehydrogenase and lactate level were estimated in rat blood samples. In addition, we calculated a number of integral coefficients of energy metabolism, such as substrate provision coefficient and coefficient of energy reactions balance. Our experiments demonstrate that 10-days course of inhalations of low nitric oxide doses (20 ppm) increases the adaptive potential of healthy rats’ organism. One of these positive effects is associated with activation of some components of energy metabolism. First of all, it realized through stimulation of catalytic activity of lactate dehydrogenase, including its erythrocyte pool. Observed metabolic effect provides the basis for pathogenic correction of diseases, associated with hypoxia, oxidative stress and energy deficiency.
The phenomenon of biocrystallization has been recorded by researchers for over a century. At the same time, despite the presence of numerous empirical data in this area, there is no unified theoretical basis for the direction under consideration. On this basis, the purpose of the review is to systematize the concepts of biocrystallomics as a new synthetic biomedical science that considers in detail and integratively the processes of bio-associated crystallization occurring both in vitro and in vivo. In this paper historical basis and current status of Russian investigations, which associated with human and animal’s biological fluids free and initiated crystallogenesis, are shown. Main stages of building, fundamental rules and problem aspects of biocrystallography as a transformation it to biocrystallomics causes are demonstrated. The definition of this science is presented, its main tasks are characterized. The main directions of biocrystallomics are described, which include experimental biocrystallomics, crystallodiagnostics, crystallopathology, crystallotropic therapy (crystallotherapy), pharmacobiocrystallomics and crystal indication. The existing methods of studying biocrystallogenesis are considered in detail, which are grouped into 3 categories: crystalloscopic, tezigraphic and experimental crystalloscopic. Special attention is paid to the factors that determine the features of the crystallization of biological fluids, among which physical, chemical, biological and mixed are distinguished. The 5 main functions of biocrystallogenesis in biological systems are revealed: protective, nutritive-metabolic, pathogenetic, informational and synthetic-biogenic. The holistic theory of biocrystallomics and its main provisions are presented. So, biocrystallomics as a new integrative science about biocrystals is characterized, and its general ways are given.
Изучали влияние динитрозильных комплексов железа (ДНКЖ) с глутатионовыми лигандами на параметры окислительного метаболизма крови крыс. Сопоставляли интенсивность липопероксидации, антиоксидантную активность и уровень малонового диальдегида в плазме крови крыс, получавших курс внутрибрюшинных инъекций физиологического раствора и раствора ДНКЖ (0,15; 0,30; 0,45 и 0,60 мМ). Показано наличие у ДНКЖ антиоксидантного эффекта, причем его выраженность демонстрирует нелинейную зависимость от их дозы с оптимумом в диапазоне 0,3–0,45 мМ.
The aim of this work is specification of biophysical technologies in estimation of human saliva dehydration structurization. Materials and methods. We study saliva specimens by 95 healthy 24—27 years old adults. Specialties of own and initiated (by 0.1%, 0.9% and 10% solutions of sodium chloride; 0.1 N hydrochloric acid solution; 0.01 N potassium hydroxide solution) saliva structurization at dehydration on hard padding were estimated with system of morphometric parameters and spectrometry investigation. The range of estimated parameters of crystallography included: crystallizability, structure index, type of interaction of crystalline and amorphous structures, facies destruction degree, uniformity of crystal distribution, expression of cellular structure, edge zone and other facies zones. To describe teziographic facies we used: main teziographic coefficient, belts coefficient crystallinity, and the rest parameters are the same ones for crystalloscopy. We used 3 wave lengths (300, 350 and 400 nm) for spectrometry of dehydrated specimens. Role of saliva temperature, pH and osmolarity in biological fluid structurization was fixed. Results. Teziocrystalloscopic and spectrometric patterns of dehydration of samples of oral fluid of healthy humans was established according to a new system of evaluation criteria. It was stated, that saliva dehydration is dynamic process of its liquid component elimination, determined by its composition. Micro- and macro-environment factors (temperature, pH, and osmolarity) affect on saliva dehydration results considerably.
Микроэлементы в медицине 18(4): 18-21 ОРИГИНАЛЬНАЯ СТАТЬЯ ИЗУЧЕНИЕ ВЛИЯНИЯ ДИНИТРОЗИЛЬНЫХ КОМПЛЕКСОВ ЖЕЛЕЗА НА НЕКОТОРЫЕ ФИЗИКО-ХИМИЧЕСКИЕ СВОЙСТВА СЫВОРОТКИ КРОВИ КРЫС А
ОРИГИНАЛЬНАЯ СТАТЬЯ ВЛИЯНИЕ ГЛУТАТИОНСОДЕРЖАЩИХ ДИНИТРОЗИЛЬНЫХ КОМПЛЕКСОВ ЖЕЛЕЗА НА НЕКОТОРЫЕ ПАРАМЕТРЫ МЕТАБОЛИЗМА КРОВИ КРЫСА
The objective of this work was to study how a prolonged course of inhalations of nitric oxide or singlet oxygen modify crystallization of the blood serum in rats. Experiments were performed with 50 adult Wistar rats, which were divided into five equal groups. The control group (n = 10) included intact rats, which were not exposed to any manipulation other than a single blood drawing. Rats of groups 2, 3, and 4 received inhalations of nitric oxide at 20, 50, and 100 ppm, respectively, daily for 30 days. Group 5 rats (n = 10) received a similar course of inhalations of singlet oxygen for 30 days. Blood samples were drawn from the sublingual vein in rats of the test groups immediately after completion of the inhalation course (day 30 of the experiment) and in the recovery period (day 60 of the experiment) and tested for crystallogenic activity. Dried samples were evaluated visually for crystallizability, structure index, facia destruction degree, and marginal zone clarity, using respective scales. A prolonged course of NO inhalations was found to modulate the crystallogenic properties of the rat serum. An optimal response was recorded at the lowest nitric oxide concentration (20 ppm). Higher NO concentrations caused more adverse changes in serum crystallization after the end of the inhalation course and hindered the recovery processes. Singlet oxygen inhalations for 30 days did not exert a considerable adverse effect on the crystallogenic properties of the rat serum.
The effects of exogenous dinitrosyl iron complexes with glutathione ligands on the parameters of oxidative metabolism in the rat blood are studied. The intensity of LPO, total antioxidant activity, and MDA level in the plasma are compared in rats receiving intraperitoneal injections of saline without and with dinitrosyl iron complexes in various concentrations (0.15, 0.30, 0.45, and 0.60 mM). Glutathione-containing dinitrosyl iron complexes are characterized by antioxidant effect that non-linearly depended of the dose with optimum at 0.30-0.45 mM.
The aim of this work was a comparative analysis of the influence of different NO forms on dehydration structurization of human blood serum. Blood specimens from 15 healthy people were treated by NO-containing gas flow (800 and 80 ppm) generated with the "Plazon" unit, experimental NO-generator (20, 50, 75 and 100 ppm) and by water solution of thiol-containing dinitrosyl iron complexes (3 mM/L). The influence of blood sodium on blood serum crystallization in original and NO-treated blood specimens was estimated. It was found, that the effect of NO on crystallogenic properties of blood serum depends directly on its concentration and form (free or bound), as well as on the presence of reactive oxygen species in gas flow. The most pronounced stimulating effect was observed for the bound form of NO--dinitrosyl iron complexes with glutathione ligands. Low NO concentrations modulated crystallogenic properties of blood serum and the most optimal stimulating action was demonstrated in gas flow containing 20 ppm nitric oxide. In contrast, high NO concentration (800 ppm) inhibited the crystallogenic activity of biological fluid with multiply increasing of structural elements destruction leading to the formation of an additional belt in marginal zone of dehydrated specimens.
Background: it is well-known that in critical conditions the general response on stimulation is common adaptation syndrome, which includes the mobilization of organism reserves and restructuring of homeostatic mechanisms. However, the action of particular agents has some specific features even under the universal adaptive response. This determines relevant features of the response of the organism, which transform the manifestation of the adaptation syndrome. Aim: The aim of this investigation is study of action of short-term physical load and epinephrine administration on crystallogenic properties of rats’ blood serum. Results: it is stated that swimming and epinephrine administration causes increasing of crystallogenic activity of rats’ blood serum. This trend is more pronounced for the first impact. In addition, both factors increase the degree of destruction of elements of the sample. Maximal elevation of facia destruction degree was fixed for the administration of epinephrine. Conclusion: we show the uniformity of response of the organism to stress stimuli of different etiology, which is manifested in the development of typical pathological processes implemented by a single algorithm regardless of the primary initiating agent.
