Aedes albopictus is a highly invasive vector for a variety of pathogens. The intensive use of insecticides has led to the widespread insecticide resistance in Ae. albopictus populations worldwide, compromising disease vector control efforts. We investigated whether the mosquito gut symbiotic bacterium Bacillus cereus reduces deltamethrin susceptibility in Ae. albopictus and elucidated the underlying mechanisms. World Health Organization (WHO) standard tube bioassays were conducted to assess deltamethrin resistance status in both laboratory and field Ae. albopictus populations before and after oral infection with Bacillus cereus_HL4.2 (B. cereus_HL4.2). We measured enzymatic activities of three major detoxification enzyme families (cytochrome P450 monooxygenases, glutathione S-transferases [GSTs], and carboxylesterases) as metabolic markers. Transcriptomic profiling via RNA sequencing (RNA-seq) identified genes differentially expressed upon B. cereus infection, with subsequent validation by quantitative reverse-transcription PCR. In vitro assays assessed the direct deltamethrin-degrading capacity of B. cereus_HL4.2, and green fluorescent protein (GFP)-labeled bacterial strains tracked bacterial persistence and transmission through mosquito developmental stages. Oral infection with B. cereus_HL4.2 significantly increased the survival rate of laboratory-susceptible Ae. albopictus after deltamethrin exposure (from 7.6 ± 2.0

Late-spring cold spells (LSCs) threaten bread wheat (Triticum aestivum L.) when cold-sensitive spike development coincides with ephemeral spring chilling. The leading Chinese cultivar Xinong 511 (XN511) exhibits superior LSC tolerance, yet the underlying developmental and molecular basis remains unclear. Phenotypic monitoring across eight sequential sampling points, designated T1-T8, revealed that XN511 displays a distinctive "slow-then-rapid" developmental trajectory relative to its parents, Xinong 2000 (XN2000) and Shaanmai 159 (SM159): an extended early vegetative phase followed by accelerated late-stage progression. This unique pattern enables temporal avoidance of the historical LSC peak window in the Yellow-Huai River Valley. To dissect the genetic circuitry, we performed time-course RNA sequencing (RNA-seq) across T1-T8, which identified 15,440 dynamically expressed genes and pinpointed T2 and T5 as two pivotal phase-transition checkpoints. Guided by these transcriptomic landmarks, we conducted whole-genome bisulfite sequencing (WGBS) precisely at T2 and T5. WGBS revealed stage-specific methylation reprogramming, identifying 172 differentially methylated regions (DMR)-associated differentially expressed genes (DEGs) at T2 and 560 at T5, including TaLNK2-3B, TaACS6-5B, and TaAP3-7D. Functional enrichment identified the SPL-AP2L aging/phase-transition pathway as a core transcriptional hub, with AP2L expression sustained longer and SPL activation delayed in XN511 relative to its parents. Collectively, our findings establish a multi-layered regulatory framework, which integrates transcriptional timing with stage-specific epigenetic reprogramming, that underpins the adaptive developmental strategy of XN511, providing both genetic and epigenetic targets for breeding wheat cultivars with improved resilience to LSCs.

Mosquito-borne diseases represent critical global public health threats. Insecticide-based prevention and interventions remain essential for disease and vector management. However, insecticide resistance in mosquitoes threatens the effectiveness of these management measures. This study investigated the susceptibility to pyrethroid insecticides and associated resistance mechanisms in five dominant mosquito populations on Hainan Island, China. World Health Organization (WHO) tube bioassays were conducted to evaluate insecticide resistance profiles in Aedes albopictus, Culex quinquefasciatus, Armigeres subalbatus, Aedes aegypti, and Culex tritaeniorhynchus. We assessed the synergistic effects of pre-exposure to 4

Blastocystis is one of the most prevalent intestinal protozoans, transmitted through the fecal-oral route. Domestic pigs and cattle serve as important reservoirs for Blastocystis, playing a crucial role in its transmission dynamics. In the present study, a PCR-sequencing tool based on the small subunit ribosomal RNA (SSU rRNA) gene was employed to investigate the prevalence and subtypes of Blastocystis in 456 pig and 302 cattle fecal samples collected in Hainan, the only tropical island province in China. The overall prevalence of Blastocystis in pigs and cattle was 30.3% (138/456) and 13.2% (40/302), respectively. Six known subtypes-ST5 (n = 139), ST21 (n = 18), ST26 (n = 10), ST10 (n = 7), ST23 (n = 2), and ST25 (n = 2)-were identified, including 138 ST5 from pigs, and 18 ST21, 10 ST26, 7 ST10, 2 ST23, 2 ST25, and 1 ST5 from cattle. A novel ST5 sequence (OQ048307) from a pig and a novel ST10 sequence (OQ048308) from a cow were detected. Our results suggest that livestock may be an important potential reservoir for zoonotic Blastocystis infection in humans and provided reliable data for future research on subtype distribution and infection control of this protozoan in tropical regions.

Water and nutrients are fundamental resources that constrain grassland productivity. Despite significant advances in our understanding of aboveground resource limitations, it remains unresolved whether these insights can be applied to belowground productivity. Here, by combining meta-analyses of 387 nitrogen (N) and 299 phosphorus (P) addition experiments with our long-term experiment in grasslands, we find that, globally, belowground productivity experiences peak N limitation under intermediate precipitation, despite N limitation on aboveground productivity intensifying with increasing precipitation. Moreover, this global pattern of belowground N limitation is mirrored in temporal dynamics. These consistent patterns suggest that belowground N limitation relies strongly and nonlinearly on spatial and temporal water variability. As grassland roots primarily function to capture water, this peak N limitation may reflect a maximal co-limitation of N and water for grassland plants under intermediate water conditions, which is also evidenced by our long-term experiment. Overall, this study highlights the divergent resource limitations of belowground versus aboveground productivity. Future changes in precipitation could significantly modify the patterns of belowground nutrient limitations, ultimately affecting grassland productivity and multifunctionality.

Traumatic brain injury (TBI) caused by external mechanical forces is a major health burden worldwide, but the underlying mechanism in glia remains largely unclear. We report herein that Drosophila adults exhibit a defective blood–brain barrier, elevated innate immune responses, and astrocyte swelling upon consecutive strikes with a high-impact trauma device. RNA sequencing (RNA-seq) analysis of these astrocytes revealed upregulated expression of genes encoding PDGF and VEGF receptor-related (Pvr, a receptor tyrosine kinase), adaptor protein complex 1 (AP-1 , a transcription factor complex of the c-Jun N-terminal kinase pathway) composed of Jun-related antigen (Jra) and kayak (kay), and matrix metalloproteinase 1 (Mmp1) following TBI. Interestingly, Pvr is both required and sufficient for AP-1 and Mmp1 upregulation, while knockdown of AP-1 expression in the background of Pvr overexpression in astrocytes rescued Mmp1 upregulation upon TBI, indicating that Pvr acts as the upstream receptor for the downstream AP-1–Mmp1 transduction. Moreover, dynamin-associated endocytosis was found to be an important regulatory step in downregulating Pvr signaling. Our results identify a new Pvr–AP-1–Mmp1 signaling pathway in astrocytes in response to TBI, providing potential targets for developing new therapeutic strategies for TBI.

Soil chromium (Cr) accumulation is escalating, severely hindering plant growth and development. Plant growth- promoting bacteria (PGPB) have shown potential in enhancing plant tolerance to heavy metals. However, the role and mechanisms of Cr(VI)-reducing PGPB strains in improving the growth of pakchoi under Cr toxicity remain unclear. This study aimed to isolate a Cr(VI)-reducing PGPB strain from Cr-contaminated soil, evaluate its effect on pakchoi growth under Cr(VI) stress, and investigate the mechanisms involved. Our findings showed that Bacillus sp. NEAU-DCB1-2 effectively reduce Cr(VI) to Cr(III) and produced indole-3-acetic acid and siderophores. Under Cr(VI) stress, inoculation with NEAU-DC1-2 significantly promoted seed germination and early growth of pakchoi. In pot experiments, NEAU-DCB1-2 significantly increased biomass accumulation, plant height, and root length of Cr(VI)-treated pakchoi seedlings, while reducing the Cr(VI) content in root, shoot and soil. Moreover, NEAU-DCB1-2 greatly increased catalase, superoxide dismutase, peroxidase, and ascorbate peroxidase activities in seedlings under Cr(VI) stress, thereby reducing malondialdehyde content. Transcriptome analysis indicated substantial alterations in gene expression patterns after inoculation with NEAU-DCB1-2 under Cr(VI) stress. Further analyses revealed that NEAU-DCB1-2 mainly affected the responses of antioxidant system, metal chelation and transport, together with auxin, abscisic acid, and jasmonic acid signaling to Cr(VI) stress. Conclusively, the Cr(VI)-reducing PGPB strain NEAU-DCB1-2 significantly enhances the growth and Cr tolerance of pakchoi through multiple mechanisms, offering a valuable microbial resource for mitigating the adverse effects of heavy metal contamination in agricultural soils on the yield and safety of vegetable crops.

Abstract Background Aedes albopictus is an important vector for pathogens such as dengue, Zika, and chikungunya viruses. While insecticides is the mainstay for mosquito control, their widespread and excessive use has led to the increased resistance in Ae. albopictus globally. Gut symbiotic bacteria are believed to play a potential role in insect physiology, potentially linking to mosquitoes’ metabolic resistance against insecticides. Methods We investigated the role of symbiotic bacteria in the development of resistance in Ae. albopictus by comparing gut symbiotic bacteria between deltamethrin-sensitive and deltamethrin-resistant populations. Adults were reared from field-collected larvae. Sensitive and resistant mosquitoes were screened using 0.03% and 0.09% deltamethrin, respectively, on the basis of the World Health Organization (WHO) tube bioassay. Sensitive and resistant field-collected larvae were screened using 5 × LC50 (lethal concentration at 50% mortality) and 20 × LC50 concentration of deltamethrin, respectively. Laboratory strain deltamethrin-sensitive adults and larvae were used as controls. The DNA of gut samples from these mosquitoes were extracted using the magnetic bead method. Bacterial 16S rDNA was sequenced using BGISEQ method. We isolated and cultured gut microorganisms from adult and larvae mosquitoes using four different media: Luria Bertani (LB), brain heart infusion (BHI), nutrient agar (NA), and salmonella shigella (SS). Results Sequencing revealed significantly higher gut microbial diversity in field-resistant larvae compared with field-sensitive and laboratory-sensitive larvae (P < 0.01). Conversely, gut microorganism diversity in field-resistant and field-sensitive adults was significantly lower compared with laboratory-sensitive adults (P < 0.01). At the species level, 25 and 12 bacterial species were isolated from the gut of field resistant larvae and adults, respectively. The abundance of Flavobacterium spp., Gemmobacter spp., and Dysgonomonas spp. was significantly higher in the gut of field-resistant larvae compared with sensitive larvae (all P < 0.05). Furthermore, the abundance of Flavobacterium spp., Pantoea spp., and Aeromonas spp. was significantly higher in the gut of field-resistant adults compared with sensitive adults (all P < 0.05). The dominant and differentially occurring microorganisms were also different between resistant larval and adult mosquitoes. These findings suggest that the gut commensal bacteria of Ae. albopictus adults and larvae may play distinct roles in their deltamethrin resistance. Conclusions This study provides an empirical basis for further exploration of the mechanisms underlying the role of gut microbial in insecticide resistance, potentially opening a new prospect for mosquito control strategies. Graphical Abstract

An 11-year nitrogen addition experiment reveals that for both plants and soil microorganisms, the ruderal strategists had higher productivity but lower stability, while the tolerant strategists had higher stability and lower productivity, leading to the tradeoff between productivity and stability within and across above- and below-ground communities.

The riparian zone ecosystems have greater energy flow and elemental cycling than adjacent terrestrial and aquatic ecosystems. Mineralization and nitrification are important initiating processes in the nitrogen cycle, but their distribution and activity under different environmental conditions in the riparian zone and the driving mechanisms are still not clear. We investigated the effects of environmental and microbial factors on mineralization and nitrification activities by analyzing the community of alkaline ( apr ) and neutral ( npr ) metallopeptidase, ammonia-oxidizing archaea (AOA), and bacteria (AOB) in soils and sediments under different land-use types in the riparian zone of Miyun Reservoir, as well as measuring potential nitrogen mineralization and ammonia oxidation rates (AOR). The results showed that the mineralization and nitrification activities of soils were greater than those of sediments. AOA and AOB dominate the ammonia oxidation activity of soil and sediment, respectively. NH 4 + content was a key factor influencing the ecological niche differentiation between AOA and AOB. The high carbon and nitrogen content of the woodland significantly increased mineralization and nitrification activity. Microbial communities were significantly clustered in the woodland. The land-use type, not the flooding condition, determined the distribution of microbial community structure. The diversity of npr was significantly correlated with potential N mineralization rates, while the transcript abundance of AOA was significantly correlated with ammonia oxidation rates. Our study suggests that environmental changes regulate the distribution and activity of mineralization and nitrification processes in the reservoir riparian zone by affecting the transcript abundance, diversity and community structure of the microbial functional genes.

A novel actinomycete, designated strain q2T, was isolated from the saline–alkaline soil, collected from Daqing, Heilongjiang province, China. The results of phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain q2T belongs to the genus Isoptericola, and showed the highest sequence similarity to Isoptericola halotolerans KCTC 19046T (98.48

Aims To understand the species composition, the relative content, and diversity of nitrogen fixing bacteria in Cenchrus fungigraminus rhizophere, and to screen nitrogen-fixing bacteria to study their potential role in plant growth promotion. Methods Soil samples were collected from 4 depth (G1, G2, G3 and G4) of C. fungigraminus rhizophere and physical and chemical properties were determined. The diversity and abundance of nitrogen-fixing bacteria and nif H gene copy were analyzed. Nitrogen-fixing bacteria were screened and selected for studying the C. fungigraminus seedlings growth promotion. Results The highest diversity and abundance of nitrogen-fixing bacteria were observed in C. fungigraminus rhizosphere (G2), which mainly included Proteobacteria (93.91%), Actinobacteria (0.42%), Firmicutes (0.18%) and significantly effected by total nitrogen, available nitrogen and depth. The nif H gene copy was also highest (1.56 ± 0.17×10 7 copies/g) in G2. Rhizobium pusense NO.8 and NO.28 were isolated in G1 and G2, respectively, with nitrogenase activity of 1.45 ± 0.04 and 2.00 ± 0.07 U/g. The promotion experiment revealed that plant height, root length, leaf length of C. fungigramminus seedlings treated with both strains were significantly increased 56.79%, 76.99% and 55.71%, and significantly increased on moisture and total nitrogen of planting soil compare with control ( P < 0.05). The available nitrogen, organic matter and organic carbon in soil with R. pusense NO.28 have significantly increased 3.09, 5.77 and 5.77 times. Conclusion Nitrogen-fixing bacteria in C. fungigraminus rhizosphere soil is rich and play a significant role in promoting its seedlings growth. The study provides the development of PGPRs from C. fungigraminus rhizosphere to be biological fertilizers.

Abstract Background Mosquitoes are vectors of many pathogens, such as malaria, dengue virus, yellow fever virus, filaria and Japanese encephalitis virus. Wolbachia are capable of inducing a wide range of reproductive abnormalities in their hosts, such as cytoplasmic incompatibility. Wolbachia has been proposed as a tool to modify mosquitoes that are resistant to pathogen infection as an alternative vector control strategy. This study aimed to determine natural Wolbachia infections in different mosquito species across Hainan Province, China. Methods Adult mosquitoes were collected using light traps, human landing catches and aspirators in five areas in Hainan Province from May 2020 to November 2021. Species were identified based on morphological characteristics, species-specific PCR and DNA barcoding of cox1 assays. Molecular classification of species and phylogenetic analyses of Wolbachia infections were conducted based on the sequences from PCR products of cox1, wsp, 16S rRNA and FtsZ gene segments. Results A total of 413 female adult mosquitoes representing 15 species were identified molecularly and analyzed. Four mosquito species (Aedes albopictus, Culex quinquefasciatus, Armigeres subalbatus and Culex gelidus) were positive for Wolbachia infection. The overall Wolbachia infection rate for all mosquitoes tested in this study was 36.1% but varied among species. Wolbachia types A, B and mixed infections of A × B were detected in Ae. albopictus mosquitoes. A total of five wsp haplotypes, six FtsZ haplotypes and six 16S rRNA haplotypes were detected from Wolbachia infections. Phylogenetic tree analysis of wsp sequences classified them into three groups (type A, B and C) of Wolbachia strains compared to two groups each for FtsZ and 16S rRNA sequences. A novel type C Wolbachia strain was detected in Cx. gelidus by both single locus wsp gene and the combination of three genes. Conclusion Our study revealed the prevalence and distribution of Wolbachia in mosquitoes from Hainan Province, China. Knowledge of the prevalence and diversity of Wolbachia strains in local mosquito populations will provide part of the baseline information required for current and future Wolbachia-based vector control approaches to be conducted in Hainan Province. Graphical Abstract

Rice direct seeding technology has been considered as a promising alternative to traditional transplanting because of its advantages in saving labor and water. However, the poor emergence and seedling growth caused by chill stress are the main bottlenecks in wide-scale adoption of direct-seeded rice in Heilongjiang Province, China. Here, we found that natural plant growth regulator guvermectin (GV) effectively improved rice seed germination and seedling growth under chilling stress. Results from 2 year field trials showed that seed-soaking with GV not only enhanced the emergence rate and seedling growth but also increased the panicle number per plant and grain number per panicle, resulting in 9.0 and 6.8% increase in the yield of direct-seeded rice, respectively. Integrative physiological, transcriptomic, and metabolomic assays revealed that GV promoted seed germination under chilling stress mainly by enhancing the activities of α-amylase and antioxidant enzymes (superoxide dismutase, peroxidase, and catalase), increasing the contents of soluble sugar and soluble protein, improving the biosynthesis of glutathione and flavonoids, as well as activating gibberellin-responsive transcription factors and inhibiting the abscisic acid signaling pathway. These findings indicate that seed-soaking with GV has good potential to improve seedling establishment and yield of direct-seeded rice even under chilling stress.

OVATE Family Protein(OFP)是一类植物特异性转录因子,广泛参与植物的生长发育,其在棉花中的研究尚未见报道.本研究从陆地棉NDM8基因组中共鉴定到61个GhOFP基因,系统聚类将其分为6个亚组(Clade Ⅰ,Ⅱ,Ⅲ,Ⅳ,Ⅴ和Ⅵ).转录组和qRT-PCR分析表明,棉纤维发育的起始和伸长阶段高调表达的GhOFP基因主要分布在Clade Ⅱ亚组,且该亚组的GhOFP基因启动子含有促进伸长生长的赤霉素和生长素响应的顺式元件;在棉纤维发育的次级细胞壁加厚阶段高调表达的GhOFP基因主要分布在Clade Ⅵ亚组,且该亚组的GhOFP基因启动子含有参与细胞壁生长的脱落酸、茉莉酸和水杨酸响应的顺式元件.陆地棉GhOFP基因家族的全基因组鉴定和分析将为进一步研究棉花GhOFP基因在棉纤维发育过程中的生物学功能提供参考.

The antihyperglycemic effect and mechanism of action of extracts, fractions and compounds from Wilbrandia ebracteata was studied. The crude extract reduced the glycemia, increased glycogen content and serum insulin in hyperglycemic rats. Also, a significant effect was observed with the n-butanol and metanol subfraction. However, the antihyperglycemic effect of the n-butanol fraction was not observed in diabetic rats. The C-glycosylflavones isovitexin and swertisin showed a strong antihyperglycemic action compared with the extracts and fractions. These results show that the extracts, fractions, and isolated C-glycosylflavones have an antihyperglycemic action that was reinforced by the stimulation on in vivo insulin secretion.

As a transitional boundary between terrestrial and aquatic ecosystems, the riparian zone is considered a hotspot for N2 O production because of the active nitrogen processes. Ammoxidation is an important microbial pathway for N2 O production, but the distribution of ammonia oxidizers under different land-use types in the reservoir riparian zone and what role they played in N2 O emissions are still not clear. We investigated spatiotemporal distributions of ammonia-oxidizing archaea (AOA) and bacteria (AOB) and their role in N2 O emissions in different land-use types along the riparian zone of Miyun Reservoir: grassland, sparse woods, and woodland. We found significant differences in both AOA abundance and AOB diversity indices among land-use types. AOA and AOB communities were significantly separated by different land-use types. The main drivers to determine the distribution of ammonia-oxidizing microbial community were soil water content, NH4 + , NO3 - , and total organic carbon (TOC). In situ N2 O flux was highest in woodland with a mean value of 12.28 μg/m2 ·h, and it was substantially decreased by 121% and 123% in sparse woods and grassland. TOC content was decreased by 20% and 40% in sparse woods and grassland compared with woodland, and it was significantly positively correlated with in situ N2 O flux. Meanwhile, AOB diversity indices were significantly correlated with in situ N2 O flux. These results showed that the heterogeneity of physicochemical properties among different land-use types affected the community of AOA and AOB in riparian zones. AOB not AOA, and community diversity rather than abundance, played a role in N2 O emissions.

Enterocytozoon bieneusi is a zoonotic pathogen commonly found in humans and animals all over the world. Here, we investigated the occurrence and genotype constitute of E. bieneusi among the individuals from Haikou city of Hainan, China. A total of 1,264 fecal samples of humans were collected, including 628 samples from patients with diarrhea (325 adults and 303 children) and 636 samples from the asymptomatic population (383 college students and 253 kindergarten children). E. bieneusi was detected using nested polymerase chain reaction (PCR) amplification of the internal transcribed spacer (ITS) region. A phylogenetic tree was constructed using a neighbor-joining tree construction method. The overall prevalence of E. bieneusi was 3.7% (47/1,264), while it was 5.6% in the patients with diarrhea (5.8% in adults and 5.3% in children) and 1.9% in the asymptomatic population (2.9% in college students and 0.4% in kindergarten children). The prevalence of E. bieneusi in humans with diarrhea was significantly higher than that in the asymptomatic population (χ2 = 36.9; P < 0.05). A total of 28 genotypes were identified, including ten known genotypes: CHG2 (n = 3), CHG3 (n = 5), CHG5 (n = 10), CM21 (n = 1), EbpA (n = 1), EbpC (n = 1), PigEBITS4 (n = 1), PigEBITS7 (n = 1), SHR1 (n = 4), Type IV (n = 2), and 18 novel genotypes (HNH-1 to HNH-18; one each). All these genotypes were categorized into three groups, including group 1 (n = 6), group 2 (n = 14), and group 13 (n = 8). This was the first study on the identification of E. bieneusi among humans in Hainan, China. The correlation between E. bieneusi infection and diarrhea was observed. The high diversity and distinctive distribution of E. bieneusi genotypes found in this study reflected the unique epidemic genetic characteristics of E. bieneusi in humans living in Hainan.

Mitochondrial genes were generally adopted for PCR-based meat adulteration authentication due to their excellent specificity to species and numerous copies in one cell. However, the number of mitochondrial gene copies varies according to cells and tissues, which leads to quantification errors for meat adulteration. To address this problem, single-copy nuclear genes were selected to develop a quantitative method for identifying mutton adulteration in this study. Both single-copy genes specific to sheep species and single-copy reference genes show good linearity between Ct values and series diluted DNA concentrations, with the correlation coefficients of 0.9999 and 0.9993, respectively. Meanwhile, a constant (correction factor) was introduced to transform DNA concentrations into mutton proportions in adulterated meat. With this method, simulated mutton-pork, mutton-chicken and mutton-duck adulteration samples could be accurately quantified with the recovery rates of 89.56%, 107.13% and 95.20%, respectively.

A bacterial strain, Gram-stain negative, rod-shaped, aerobic and cellulose-degrading, designated NEAU-DD11T, was isolated from rhizosphere soil of rice collected from Northeast Agricultural University in Harbin, Heilongjiang Province, North-east China. Base on 16S rRNA gene sequence analysis, strain NEAU-DD11T belongs to the genus Massilia and shared high sequence similarities with Massilia phosphatilytica 12-OD1T (98.46%) and Massilia putida 6NM-7 T (98.41%). Phylogenetic analysis based on the 16S rRNA gene and whole genome sequences indicated that strain NEAU-DD11T formed lineage related to M. phosphatilytica 12-OD1T and M. putida 6NM-7 T. The major fatty acids of the strain were C16:0, C17:0-cyclo and C16:1ω7c. The respiratory quinone was Q-8. The polar lipids profile of the strain showed the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified polar lipid and an unidentified phospholipid. In addition, the digital DNA-DNA hybridization values between strain NEAU-DD11T and M. phosphatilytica 12-OD1T and M. putida 6NM-7 T were 45.4 and 35.6%, respectively, which are lower than the accepted threshold value of 70%. The DNA G + C content of strain NEAU-DD11T was 66.2%. The whole genome analysis showed the strain contained carbohydrate enzymes such as glycoside hydrolase and polysaccharide lyase, which enabled the strain to have the function of degrading cellulose. On the basis of the phenotypic, genotypic and chemotaxonomic characteristics, we conclude that strain NEAU-DD11T represents a novel species of the genus Massilia, for which the name Massilia cellulosiltytica sp. nov. is proposed. The type strain is NEAU-DD11T (= CCTCC AB 2019141 T = DSM 109721 T).