A series of 5-benzylidene-5H-[1,2,3]triazolo[5,1-b][1,3,4]thiadiazines were synthesized by the reaction of benzaldehyde 1,2,3-thiadiazol-5-ylhydrazones with a-bromo-4-chloroacetophenone and chloroacetone in the presence of triethylamine. The key steps of the reaction are the Dimroth rearrangement of the 1,2,3-thiadiazole ring, the formation of 6,7-dihydro-5H-[1,2,3]triazolo[5,1-b][1,3,4]thiadiazines, and the transformation of the thiadiazine ring giving 5-arylidene-5H-[1,2,3]triazolo[5,1-b][1,3,4]thiadiazines. It was demonstrated that 6,7-dihydro-5H-[1,2,3]triazolo[5,1-b][1,3,4]thiadiazines, which do not undergo the transformation in the presence of triethylamine, can be transformed into 5-arylidene-5H-[1,2,3]triazolo[5,1-b][1,3,4]thiadiazines using sodium hydroxide. The reaction is accompanied by the saponification of the ethoxycarbonyl substituent. A number of the synthesized compounds were tested for fungicidal activity against four strains of phytopathogenic fungi and the oomycete P. infestans. Compound 5n exhibits a moderate inhibitory effect (56.31±0.13

Целью исследования является оценка влияния производного 1,2,3-триазоло-1,3,4 тиадиазина на восстановление волокнистого компонента кожи в условиях заживления ожоговой раны. В качестве экспериментальных животных использовали самцов белых беспородных лабораторных крыс. Были проведены морфологические и механические исследования. Животных опытных групп выводили из эксперимента на 14-е и 21-е сутки для оценки течения регенераторного процесса. Опытным животным моделировали ожоговую рану кожи IIIА степени. Лечение осуществлялось нанесением на пораженный участок мази, содержащей исследуемое вещество. Данные морфологического анализа свидетель ствуют о стимулирующем влиянии 1,2,3-триазоло-1,3,4-тиадиазина на регенерацию кожи, в частности, восстановление волокнистого компонента. К 14-м суткам эксперимента все образцы имеют слабые механические характеристики. Однако на 21-е сутки эксперимента образцы из группы с тестируемым веществом демонстрируют более высокие прочностные свойства, растягиваясь на 28,893 ± 4,548 % при максимальной нагрузке в 0,804 ± 0,129 МПа. Достоверное различие с интактным значением (кожа до разрыва растягивается на 31,434 ± 4,921 % при нагрузке в 5,082 ± 0,060 МПа) связано, вероятно, с тем, что механические свойства кожи определяются не только толщиной коллагеновых волокон, но и способом укладки волокон в пучки, взаимным расположением и взаимодействием с другими структурными компонентами ткани.

A computer-aided approach has been used to predict the activity of new antibacterial compounds. In this study, pharmacophore and QSAR models to identify novel FtsZ targeting compounds have been developed. The virtual screening based on the models of proprietary library ASINEX predicted two compounds as potential anti-Staphylococcus aureus agents with the capability to bind to FtsZ in the pocket binding located between the C-terminal domain and helix (H7).

BACKGROUND Plant elicitors are a class of plant protection agents that can stimulate plant immunity against phytopathogen without a potential resistance problem. In searching for novel plant elicitor candidates, a series of novel 1,2,3-thiadiazole and isothiazole-based phenyl substituted pyridine containing carboxamides were designed and synthesized. RESULTS In vitro bioassay showed that all new compounds exhibited weak direct fungicidal activity. However, compounds 3b, 3 g, 3n and 3o showed a broad spectrum of in vivo activity against four plant fungi tested. In particularly, 3 g showed 80% activity against Rhizoctonia solani in greenhouse at a concentration of 1 μg/mL. For induction activity of tobacco against tobacco mosaic virus (TMV), compounds 3c and 3v showed 67% and 68% inhibitory activity, respectively, which were superior to the positive controls ribavirin and ningnanmycin. Compound 3 g showed moderate induction activity (41%). RT-q-PCR analysis found that, 3 g could up-regulate expression of genes that are related to reactive oxygen species (ROS), pathogenesis-related protein (PRP) and salicylic acid (SA) signalling. CONCLUSION These results indicated that 3 g as an elicitor candidate might act on the SA signalling pathway. According to our findings, 1,2,3-thiadiazole and isothiazole-based phenyl substituted pyridine containing carboxamides might be promising lead scaffolds as a novel plant elicitor for further investigation. This article is protected by copyright. All rights reserved.

The biological activity of 5-benzimidazol-2-yl carbamate derivatives is associated with their ability to form a complex with /3-tubulin and, consequently, disrupt the process of assembly of microtubules of the cytoskeleton of cells. Over the past 10 years, the understanding of the binding of 5-benzimidazol-2-yl carbamate derivatives to the /3-tubulin subunit has increased significantly, mainly due to the published crystal structures of their ligand-receptor complexes. However, some details of this process could be predicted based on the results of molecular modeling before the publication of the corresponding crystal data. This mini-review summarizes the results of such works.

The synthesis of new isatin derivatives containing the 1,2,3-thiadiazole ring in the hydrazone fragment was carried out. It was shown that the obtained compounds do not possess a hemolytic effect and do not exhibit cytotoxicity with respect to normal Chang liver human cell lines.

The effect of two synthesized compounds of the 1,2,3-triazolo[5,1-b][1,3,4]thiadiazine class as well as three known plant growth regulators (gibberellic acid, thidiazurone and 6-benzylaminopyrine)on seed germination and growth of seedlings of B. napus of the “Heros” linear variety was studied. It was shown that treatment with gibberellic acid slowed the germination of seeds especially at the concentration of 10 mg/L. Treatment with TT1 and TT2 compounds at a concentration of 5 mg/L had the best effect on seed germination and seedling growth.

In this paper the results of the in vitro study of synthesized derivatives 1,2,3-triazolo-1,3,4-thiadiazine on morpho- and rhizogenesis, as well as tuberization of microplants of the "Irbitskiy" potato variety are described. It has been shown that the use of compounds of this series is very promising for acceleration of growth and development of explants. In this study, substance T6 showed the best results. By the number of rudiments of tubers on the stolons, plants grown on nutrient media with T3 were prominent, however, by quality indicators for nutrient media with T6 was better. 1,2,3-Triazolo-1,3,4-thiadazine T6, which showed the most pronounced results, is recommended for further study of the possibility of its use in potato production.

The possibility of regulation of textural parameters for porous carbon materials derived from polyvinyl chloride was evaluated. Porous carbon materials were obtained by a two-stage process including dehydrochlorination of the initial polymer under the action of alkali (KOH) and subsequent heat treatment, i.e., carbonization at 400 degrees C and activation at 850-900 degrees C. Various approaches for controlling the porous structure of the final material were used. Thus, dehydrochlorination was carried out both by action of alkali in a solution in polar organic solvent and by mechanical treatment of polymer-alkali composition. In the case of dehydrochlorination in a solution, modifying additives of carbon nanoparticles (nanoglobular carbon, graphene-like species based on graphite oxide or reduced graphite oxide) were introduced. Different gases of reductive and oxidative nature, such as hydrogen, carbon dioxide and water vapor, were used for activation purpose. It was found that these approaches allow regulating the ratio of micro- and mesopores in the resulting porous carbon materials which can be used in different fields. These porous carbons are amorphous materials having a specific surface area (S-BET) from 356 to 1115 m(2)/g, micropore volume from 0.12 to 0.39 cm(3)/g, and mesopore volume from 0.07 to 0.33 cm(3)/g.

An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.

The effects of the 5′-(4-ethoxybenzoyl)-5′,7′-dihydrospiro[cyclopentane-1,6′-[1,2,3]-triazolo[5,1-b][1,3,4]-thiadiazine]-3′-carboxylic acid ethyl ester oon the healing of linear skin wounds were studied in rats. This compound was found to have a stimulatory action on skin fibroblast proliferation, increasing the regenerative potential of damaged tissue. Assessment of the course of the wound process showed that use of test substance clearly accelerated the course of repair regeneration of the integument as compared with controls; inflammatory processes were moderate, without involvement of the deep layers of the skin; a cellular and fibrous component formed in the lesion zone.

One of the modern trends in the field of plant protection is use an activators of systemic acquired resistance of plants. In order to search for compounds capable of stimulating systemic acquired resistance of plants, 1,2,3-selenodiazolylureas derivatives, structural analogues of commercial SAR activator tiadinil were obtained, their ability to induce, as well as antiviral activity against tobacco mosaic virus were studied. Revealed compounds that have biological activity at the level of tiadinil and above.

Novel unknown potentially biologically active 1-(1,2,3-thiadizolylcarbonyl)-4-(1,2,3-thiadiazolyl)-semicarbazides were synthesized by reactions of 1,2,3-thiadiazolylcarboxylic acid hydrazides with 1,2,3- thiadizolylcarbonyl azide in high yields. The structure of the synthesized compounds was studied by NMR and IR spectroscopy and X-ray diffraction analysis.

An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.

Результаты были получены в рамках гранта Российского фонда фундаментальных исследований (грант № 16-33-00556 мол_а).