EXPERIMENTAL SUBHEPATIC OBSTRUCTIVE JAUNDICE AND BCL-2 GENE EXPRESSION

The aim of the study was to investigate lipid peroxidation and antioxidant defense state in different parts of rat small intestine in the course of experimental subhepatic obstructive jaundice. The experiment was carried out on 65 mongrel white male rats weighting 250 ± 30g. A total of 4 sets of experimental subhepatic obstructive jaundice models of different duration were produced. False operated rats served as a control group. The obtained data show that prolonged ligation of the common bile duct leads to a steady and significant elevation of common bile acid serum concentration, producing cytotoxic effects on parenchymatous elements of small intestine layers on different levels, which, in turn, causes changes in lipid peroxidation and antioxidant defense state in different parts of the small intestine.

The experiment was carried out on 55 white outbread male rats weighing 250±50g. The results of the complex investigation showed that in surviving rats a 30-day cholestasis resulted in secondary involvement of the liver parenchyma and serious disorder of its exocrine function. There was a marked increase in concentration of surface-active bile components in blood serum of these animals (bile acids, common bilirubin) as well as in alanine-aminotransferase activity while aspartate aminotransferase activity decreased. Under marked cholaemia kidneys removed excess bile acids from the body and stable choluria developed. The processes of lipid peroxidation were activated in renal homogenates and antioxidant defense of the organ decreased. Reduced activity of SDH, increased activity of the lysosomal marker enzyme acid phosphatase, hydropic degeneration were observed in the cytoplasm of proximal tubule epithelial cells in both cortical and juxtamedullary nephrons which appeared to be a direct evidence of serious morphological changes.

The review presents the data about the influence of biliary system pathology on the development of changes in the endocrine organs which may have serious consequences for the whole body as hypothalmic-pituitary-adrenal axis disorders evidence the presence of multiple organ failure.

Experiments were performed in 38 rat pups at the age of 15-45 days, 22 of these rats were born from female rats with obturative cholestasis experimentally induced on the 17 th day of pregnancy. The neutrophilic phagocytic activity, complement activity and indices of prooxidant-antioxidant balance were studied. The results showed that the maternal experimental cholestasis developed during the active phetogenesis caused in the litter the steady depression of neutrophilic phagocytic activity in blood, decrease in complement system parameters, more active lipid peroxidation in gastric and renal tissue and the lower a-tocopherol content and catalase activity. The degree of such changes varied depending on organ and age. We investigated the neutrophil phagocytic and complement activity and indices of prooxidant-antioxidant balance in 38 rat pups (15and 45-day-old). Maternal cholestasis experimentally induced on the 17 th day of pregnancy (during the active fetogenesis) led to the prolonged inhibition of neutrophil phagocytic activity, lower levels of complement system substances, more active lipid peroxidation in gastric and renal tissues combined with their lower a-tocopherol content and catalase activity. The degree of such changes depended on organ and age.

The experiments were carried out on 17 rat pups (age 15 days) 10from them were born under conditions of obturational cholestasis induced in their mothers on the 12th day of pregnancy. Using anthropometric, histologic and morphometric methods, we showed that the experimentally induced cholestasis caused both lower weight gain in pregnant females and their litter and retardation of the physical development and of cellular differentiation in parenchyma of gastrointestinal and urogenital systems in rat pups. The degree of such changes depended both on the type of organ and on its functional activity during this age period.