为研究槐角乙酸乙酯部位的化学成分,采用槐角粗粉70％乙醇提取物,正己烷、乙酸乙酯、正丁醇分步萃取,利用硅胶柱、Sephadex LH-20凝胶柱、半制备高效液相等色谱技术进行分离纯化,根据化合物的理化性质、波谱数据并结合文献报道鉴定化学物的结构.结果可知,从槐角乙酸乙酯部位中分离得到18个化合物,分别鉴定为鹰嘴豆芽素A(1)、红车轴草素(2)、樱黄素(3)、木豆素(4)、木樨草素(5)、香叶木素(6)、3′-甲基香豌豆酚(7)、2′-羟基鹰嘴豆芽素A(8)、杨梅素(9)、柚皮素(10)、芫花素(11)、芹菜素(12)、杨梅苷(13)、白杨素(14)、杨属苷(15)、异槲皮苷(16)、黄芩苷(17)、金丝桃苷(18).所有化合物均为首次从槐角中分离得到,其中化合物7、8、9、11、15、18为首次从槐属植物中分离得到.

目的:优化人参糖蛋白的制备工艺.方法:利用Box-Behnken响应面法分析提取时间、提取次数、料液比、乙醇体积分数对人参糖蛋白的影响.结果:最优人参糖蛋白制备工艺为提取时间1.5 h、提取次数3次、料液比(m人参粗粉∶V蒸馏水)1.0∶ 15.4(g/mL)、乙醇体积分数72％,该条件下人参糖蛋白质量分数为24.14％.结论:优化的人参糖蛋白的制备工艺稳定性好、准确可靠、提取率高,具有一定的实用价值.

蒿属植物是菊科中种类最多、分布最广的属之一.黄酮类化合物广泛存在于蒿属植物中,是一类重要的天然有机化合物.本文对近10年来国内、外有关蒿属植物的黄酮类成分及其药理活性研究进展进行了综述,为蒿属植物中黄酮类物质的进一步研究与开发提供参考.

文章对人参糖肽的提取分离方法、检测分析方法、结构鉴定、功能活性等内容进行了综述,并对其未来发展方向进行了展望.

A ultra-performance liquid chromatography tandem mass spectrometry method was initially developed and validated for quantification of sophorabioside in rat plasma using kaempferol-3-O-β-D-rutinoside as the internal standard (IS). Analyte and IS were preparation through a protein precipitation procedure with 1.0 mL of methanol to a 0.1 mL plasma sample. The processed samples were separated by C18 analytical column using methanol/water containing 0.1% formic acid with gradient elution as the mobile phase at a flow rate of 0.3 mL/min. Sophorabioside (m/z 577.15 → 269.45) and kaempferol-3-O-β-D-rutinoside (m/z 593.15 → 285.84) were detected by a triple quadrupole tandem mass spectrometer in negative electrospray ionization mode using multiple reaction monitoring. The calibration curve for sophorabioside was linear in the range of 6-1,200 ng/mL (r2 > 0.995) with a lower limit of quantification of 6 ng/mL. The inter- and intra-day precision and accuracy were well within the acceptable limits. The matrix effects were satisfactory in all of the biological matrices examined. The mean recovery of sophorabioside was always >90%. This method was successfully applied to a pharmacokinetic study of sophorabioside in rats after an oral administration of 90 mg/kg sophorabioside. The main pharmacokinetic parameters: Tmax, Cmax and t1/2 were 6.2 ± 0.8 h, 1430.83 ± 183.25 ng/mL, 7.2 ± 0.5 h, respectively.