Through the aseptic culture test of Sphaeropteris lepifera (J Sm. ex Hook.) R. M. Tryon, try to screen out the best conditions for each process of aseptic culture for spores of Sphaeropteris lepifera, in order to establish a systematic, efficient and complete aseptic culture technology system for spores of Sphaeropteris lepifera, and try to achieve factory production of Sphaeropteris lepifera seedlings. The mature dark brown spores off leaf from Sphaeropteris lepifera were used as explants, disinfected with 75% alcohol for 10 s and 0.1% Hg for 6 min. The induced germination medium is 1/5 MS+6-BA 0.5 mg/L+IBA 0.5 mg/L. The proliferation medium is 1/2 MS + 6-BA 0.3 mg/L+ IBA 0.05 mg/L. The differentiation medium is MS+6-BA 0.05 mg/L+ IBA 0.05 mg/L. And the rooting medium is MS + 6-BA 0.2 mg/L + IBA 0.2 mg/L. The culture temperature is (25±2)°C, the light source is fluorescent, the illumination time is 12 h/d, and the illumination intensity is 2000 lx.
通过对笔筒树为期1a的连续观测,揭示其年生长发育规律.结果 表明:在厦门地区的笔筒树植株生长发育全年无休眠,4—7月和9—10月为营养生长高峰期,11月至翌年3月新生叶片较少,营养生长的最适宜温度为25~28℃;1—2月和4—7月为生殖生长高峰期,8—12月的新生孢子叶较少,生殖生长的适宜温度为15~28℃.植株在树荫较多、 空气潮湿的地方营养生长较好,而阳光充足条件则有利于孢子生长.孢子囊群成熟期25 d左右.植株的年新增叶片数与其干高有显著正相关性.