Through the aseptic culture test of Sphaeropteris lepifera (J Sm. ex Hook.) R. M. Tryon, try to screen out the best conditions for each process of aseptic culture for spores of Sphaeropteris lepifera, in order to establish a systematic, efficient and complete aseptic culture technology system for spores of Sphaeropteris lepifera, and try to achieve factory production of Sphaeropteris lepifera seedlings. The mature dark brown spores off leaf from Sphaeropteris lepifera were used as explants, disinfected with 75% alcohol for 10 s and 0.1% Hg for 6 min. The induced germination medium is 1/5 MS+6-BA 0.5 mg/L+IBA 0.5 mg/L. The proliferation medium is 1/2 MS + 6-BA 0.3 mg/L+ IBA 0.05 mg/L. The differentiation medium is MS+6-BA 0.05 mg/L+ IBA 0.05 mg/L. And the rooting medium is MS + 6-BA 0.2 mg/L + IBA 0.2 mg/L. The culture temperature is (25±2)°C, the light source is fluorescent, the illumination time is 12 h/d, and the illumination intensity is 2000 lx.

为了解姜花属(Hedychium)植物的抗寒性并筛选抗寒种质,测定了3种姜花属植物和11个品种在自然越冬过程中的相对电导率、可溶性蛋白质和MDA含量及SOD、POD活性的变化,采用主成分分析结合隶属函数法对姜花属植物抗寒性进行综合评价及聚类分析.结果表明,姜花属植物的5个生理指标随越冬时间的延长均呈先上升后下降的变化趋势,多数于气温最低月(2月)达到最大值.同一时期,抗寒性强的种类或品种的相对电导率和MDA含量较低,而SOD和POD活性及可溶性蛋白含量则较高.SOD活性与MDA和可溶性蛋白含量分别呈显著负相关和极显著正相关;MDA含量与可溶性蛋白含量呈极显著负相关;相对电导率与MDA含量呈极显著正相关.主成分分析表明,5个指标可转化为2个主成分,累积贡献率达81.29％.综合评价结果表明,姜花属植物的抗寒性由强到弱为:'橙汁'姜花、'橙塔'姜花、'活宝'姜花、'黄白'姜花、白姜花、'西里岛'金姜花、'伊丽莎白'姜花、'粉红色'姜花、峨眉姜花、'塔拉'金姜花、'绝色'姜花、'美梦成真'姜花、'白星'姜花、'星爆'姜花.这为筛选姜花属优良的抗寒种类和品种资源的推广与应用提供了理论和实践依据.