Sugarcane (Saccharum spp.) is a vital sugar and bioenergy crop with an exceptionally complex polyploid genome (10-12 sets of chromosomes). This complexity resulted from nobilization-a historical breeding process involving interspecific hybridization and repeated backcrossing1. However, the extreme ploidy has long impeded efforts to elucidate the genetic basis of its considerable sucrose-storing capacity. Here we present a fully phased genome assembly of the foundational cultivar POJ2878, achieved using a Pore-C-based assembly algorithm. This assembly resolved 118 chromosomes, revealing extensive subgenome recombination and non-homologous chromosomal rearrangements. Using identity-by-descent and allele-specific expression profiling, we identified breeder-favoured haplotypes, including a SUS2 haplotype with enhanced sucrose content. Resequencing of 981 Saccharum accessions traced POJ2878's pervasive contribution to modern cultivars and identified key domestication and improvement sweeps. Genes under selection include CBL1 for cold tolerance, TIP1 for cell size regulation and TB1 for tillering control. A genome-wide association study tailored for polyploid genomes resolved loci associated with parenchyma cell size and sucrose storage capacity, including the functionally validated sucrose transporter Saccharum hybrid SUT2. These findings clarify the genetic architecture underlying sugarcane's biomass productivity and sugar yield, offering a genomic foundation for accelerating improvement in sugarcane and other polyploid crops critical for global food and bioenergy security.

This study aims to understand the characteristics and distribution patterns of soil phosphorus (P) forms in the tropical high-P orchards under cover cropping and to explore the biotic and abiotic factors driving the changes in P forms. The study collected three kinds of soil (clean tillage [CK, control], Stylosanthes guianensis cover [SC, legume], and Cynodon dactylon (L.) Pers. cover [CC, grass]) from a mango orchard and determined P forms in the water-stable aggregates and bulk soil, analyzed the environmental factors and the structure of the bacterial-fungal community. Compared to CK, SC and CC have a significant positive influence on the forms and distribution of soil P, increase the content of organic P (Org-P) and certain inorganic P forms (Al-P and Fe-P), and enhance the content of P within macroaggregates (>0.25 mm), thereby maintaining total P (TP) and Olsen-P in the top 30 cm of soil, particularly for the SC treatment. SC and CC also improved acid phosphatase (ACP), and water-stable aggregates while decreasing bulk density (BD) and exchangeable aluminium (Al-e) in the topsoil (0-15 cm). Redundant and Pearson analysis revealed these factors significantly affected soil P availability. Moreover, SC and CC increased the relative abundance of Nitrospira, Candidatus-Udaeobacter, Pseudolabrys, and ADurb.Bin063-1 in the topsoil (0-15 cm), and decreased the relative abundance of Occallatibacter. Redundant and Pearson analysis indicated that these bacterial communities are likely associated with the availability of P. Overall, cover cropping promoted the transformation and distribution of P forms by altering the physical, chemical, and biological environment of the soil, which was beneficial for the sustainable P management in tropical high-P soils. This research offers practical insights into the use of cover crops as a tool for enhancing soil health and sustainable P management in tropical high-P orchards.

It is of great importance to study the changes in reference evapotranspiration (ET0) and the factors that influence it to ensure sustainable and efficient water resource utilization. Daily ET0 data calculated using the Penman–Monteith method from 37 meteorological stations located within Guangdong Province in the humid zone of southern China from 1960 to 2020 were analyzed. The trend analysis and Mann–Kendall test were used to analyze the time series changes in ET0 and major climatic factors (air temperature (T), relative humidity (RH), sunshine duration (SD), and wind speed (u2)) for over 61 years. Sensitivity and contribution analyses were used to evaluate the driving factors of ET0. The main findings of the study are as follows: (1) the trend in average annual ET0 time series in Guangdong slightly increased at a trend rate of 1.61 mm/10a over the past 61 years, with most stations experiencing an increase in ET0. During the same period, air temperature significantly increased, while RH and SD decreased; u2 also decreased. (2) Sensitivity analysis showed that ET0 was more sensitive to RH and T than SD and u2, with ET0 being most sensitive to RH in spring and winter and T in summer and autumn. (3) The contribution analysis showed that T was the dominant factor for ET0 variation in Guangdong, followed by SD. SD was found to be the dominant factor in ET0 changes in areas where the “evaporation paradox” occurred, as well as in spring and summer. The study concludes that the climate in Guangdong became warmer and drier over the past 61 years, and if the current global warming trend continues, it will lead to higher evapotranspiration and drought occurrence in the future.

Phosphorus (P) is one of the mineral nutrients that exhibit a high propensity for fixation in soil. Interplanting herbage is considered as a mode to produce green orchards that can effectively improve soil quality and ecological function. To assess the impacts of intercropping herbage on the morphological characteristics and availability of P in the acidic soil of mango ( Mangifera indica L . ) orchards, three kinds of soil (clean tillage [CK], intercropping Stylosanthes guianensis [SC], and intercropping Cynodon dactylon (L.) Pers. [CC]) were collected from a tropical mango orchard during a 4-year field study. This study determined the morphological characteristics of P in the water-stable aggregates and bulk soil, analysed the bacterial-fungal community structure and diversity, and evaluated the correlation between the environmental factors, P fractions, and microbial communities. This study showed that interplanting S. guianensis and C. dactylon enhanced the availability of soil P by increasing the organic P, and partial inorganic P (Al-P and Fe-P) in the 0.25–2 mm aggregate compared with the CK. In addition, interplanting herbage also altered the structure and diversity of soil bacterial-fungal community. A Mantel analysis revealed that the structure of the bacterial community had a greater influence on the P fractions compared to bacterial α-diversity. The fungal community had minimal impact on the P fractions. Interestingly, the Nitrospira , Candidatus-Udaeobacter , Pseudolabrys , MND1 , Tepidisphaera , Aquicella , unclassified-Vicinamibacterales , ADurb.Bin063‒1 , Humicola , and Purpureocillium contribute to the availability of soil P. In conclusion, interplanting herbage favors the activation of soil P in the acidic soil of mango orchards.

为实现甘蔗新品种耐旱性的早期评价鉴定,加快甘蔗耐旱育种进程.本研究以生产上已有的耐旱品种新台糖 22 号与非耐旱品种新台糖 16 号作为对照,对 2个甘蔗新品种热甘 1号与桂柳 07150 开展盆栽模拟干旱与田间干旱试验,测定叶绿素荧光特性与经济性状.结果表明,在桶栽模拟干旱胁迫下,只有新台糖 16 号的潜在最大光能利用效率 Fv/Fm显著下降,非调节性能量耗散 Y(NO)则表现为:桂柳07150＜热甘 1号＜新台糖 22 号＜新台糖 16 号,而Y(NPQ)和PARsat均表现为:2个新品种＞新台糖 22号＞新台糖 16 号.上述结果表明在土壤相对含水量rSWC降至 60%时,只有新台糖 16 号出现实质性光损伤,2 个新品种的光保护能力优于新台糖 22 号.综上,将供试品种的耐旱性由强到弱的排序为:桂柳 07150＞热甘 1号＞新台糖 22 号＞新台糖 16 号.田间观测结果表明,相同干旱胁迫程度下,Jmax与单茎重、产量、田间锤度及有效茎数均呈正相关,据此推论较强的光合能力是桂柳 07150 的单茎重与热甘 1 号的有效茎数显著高于新台糖 22 号的重要因素,并最终确定 2个新品种的产量与田间锤度显著高于新台糖 22 号,进一步验证了 2个甘蔗新品种应对干旱的能力优于新台糖 22 号.综上,叶绿素荧光技术能够在甘蔗新品种耐旱性的早期快速鉴定评价中发挥重要作用.

Mulching management is one of the most effective measures to alleviate soil erosion and improve soil quality in clean tillage orchards. However, the effects of mulching management patterns on carbon and nitrogen distribution have not been fully investigated, and water infiltration in the system is still unclear. This study aims to clarify the effects of mulching management on soil aggregates, carbon and nitrogen distribution, and water infiltration in subtropical clean tillage orchards in China. In this study, we focused on a typical latosol mango orchard in China subject to subtropical monsoon climate conditions. Traditional bare soil cleaning tillage management was applied to the control area, and two treatments (i.e., Stylosanthes guianensis covering and horticultural ground fabric mulching) were set up. Soil aggregates and aggregate-associated carbon and nitrogen were investigated in three soil depths (0–15 cm, 15–30 cm, and 30–45 cm). The water content was monitored in 0–100 cm soil layer. Compared to clean tillage (CK), horticultural ground fabric mulching (GC) and Stylosanthes guianensis covering (SC) significantly improved the stability of soil aggregates and the proportion of > 0.25-mm water-stable aggregates in the three soil layers. And SC increased the SOC contents by 11.1% and 23.2% in the 0–15 cm and 15–30 cm soil layer while increased the TN contents by 8.3 and 24.6%, respectively. Moreover, it increased the SOC stock by 18.2% in the 15–30 cm soil layer. GC only increased 17.4% in SOC content in the 15–30 cm layer but no significant changes on SOC stocks, TN contents, and stocks in the three soil layers. However, GC is conducive to the water storage in the 0–100 cm soil layer and infiltration into the > 100 cm soil layer compared to SC. In conclusion, covering Stylosanthes guianensis seems to have the best enhancing effect on soil quality in subtropical orchards but restriction the water into the > 100 cm soil layer. And small macroaggregates (0.25‒2 mm) favored the enhancement of the SOC and TN contents. The findings may be useful for clean tillage orchard management in subtropical China.

Sugarcane is the major sugar-producing crop worldwide, and hybrid F1 populations are the primary populations used in breeding. Challenged by the sugarcane genome’s complexity and the sucrose yield’s quantitative nature, phenotypic selection is still the most commonly used approach for high-sucrose yield sugarcane breeding. In this study, a hybrid F1 population containing 135 hybrids was constructed and evaluated for 11 traits (sucrose yield (SY) and its related traits) in a randomized complete-block design during two consecutive growing seasons. The results revealed that all the traits exhibited distinct variation, with the coefficient of variation (CV) ranging from 0.09 to 0.35, the Shannon-Wiener diversity index (H′) ranging between 2.64 and 2.98, and the broad-sense heritability ranging from 0.75 to 0.84. Correlation analysis revealed complex correlations between the traits, with 30 trait pairs being significantly correlated. Eight traits, including stalk number (SN), stalk diameter (SD), internode length (IL), stalk height (SH), stalk weight (SW), Brix (B), sucrose content (SC), and yield (Y), were significantly positively correlated with sucrose yield (SY). Cluster analysis based on the 11 traits divided the 135 F1 hybrids into three groups, with 55 hybrids in Group I, 69 hybrids in Group II, and 11 hybrids in Group III. The principal component analysis indicated that the values of the first four major components’ vectors were greater than 1 and the cumulative contribution rate reached 80.93%. Based on the main component values of all samples, 24 F1 genotypes had greater values than the high-yielding parent ‘ROC22’ and were selected for the next breeding stage. A rapid sucrose yield estimation equation was established using four easily measured sucrose yield-related traits through multivariable linear stepwise regression. The model was subsequently confirmed using 26 sugarcane cultivars and 24 F1 hybrids. This study concludes that the sugarcane F1 population holds great genetic diversity in sucrose yield-related traits. The sucrose yield estimation model, ySY=2.01xSN+8.32xSD+0.79xB+3.44xSH−47.64, can aid to breed sugarcane varieties with high sucrose yield.

Selections of drought-tolerant cultivars and drought-stress diagnosis are important for sugarcane production under seasonal drought, which becomes a crucial factor causing sugarcane yield reduction. The main objective of this study was to investigate the differential drought-response strategies of drought-resistant (‘ROC22’) and -susceptible (‘ROC16’) sugarcane cultivars via photosynthetic quantum efficiency (Φ) simulation and analyze photosystem energy distribution. Five experiments were conducted to measure chlorophyll fluorescence parameters under different photothermal and natural drought conditions. The response model of Φ to photosynthetically active radiation (PAR), temperature (T), and the relative water content of the substrate (rSWC) was established for both cultivars. The results showed that the decreasing rate of Φ was higher at lower temperatures than at higher temperatures, with increasing PAR under well-watered conditions. The drought-stress indexes (εD) of both cultivars increased after rSWC decreased to the critical values of 40% and 29% for ‘ROC22’ and ‘ROC16’, respectively, indicating that the photosystem of ‘ROC22’ reacted more quickly than that of ‘ROC16’ to water deficit. An earlier response and higher capability of nonphotochemical quenching (NPQ) accompanied the slower and slighter increments of the yield for other energy losses (ΦNO) for ‘ROC22’ (at day5, with a rSWC of 40%) compared with ‘ROC16’ (at day3, with a rSWC of 56%), indicating that a rapid decrease in water consumption and an increase in energy dissipation involved in delaying the photosystem injury could contribute to drought tolerance for sugarcane. In addition, the rSWC of ‘ROC16’ was lower than that of ‘ROC22’ throughout the drought treatment, suggesting that high water consumption might be adverse to drought tolerance of sugarcane. This model could be applied for drought-tolerance assessment or drought-stress diagnosis for sugarcane cultivars.

探究地区气温、降水等气候资源变化特征,可为气候变化下农业生产规划布局和气象灾害防御提供科学依据.利用1960-2020年海南岛7个气象站逐日气象资料,采用气候倾向率法、Mann-Kendal(MK)检验和Morlet小波分析等方法,对海南岛近61 a气温、降水及参考作物蒸散量(ET0)的时空变化趋势进行了系统分析.结果表明:在时间上,海南岛年平均气温以0.22℃/(10 a)的速率显著上升,年平均降水量以33.66 mm/(10 a)的速率不显著增加,ET0以-0.30 mm/(10 a)的速率轻微下降.MK检验表明气温序列未发生突变,而降水和ET0序列存在突变现象.小波分析表明气温存在55 a的变化主周期,降水和ET0存在56 a的主周期.在不同季节,气温均显著上升,降水和ET0的变化趋势不显著.在空间上,海南岛气温以中部琼中为低值中心向周边沿海地区增加,东南部气温整体高于东北部;降水量以中部琼中为中心向周边沿海地区减小,ET0呈从东北向西南递增的趋势.研究表明近61 a海南岛气候呈现暖湿化趋势,对全球变暖响应显著.预测未来几年气温、降水和ET0将处于偏低期,这将对该地区农业生产和生态系统等产生影响.

[目的]利用转录组测序开发甘蔗SNP分子标记,为甘蔗建立一种高效和低成本的分子标记开发方法.[方法]以40个甘蔗栽培品种为试验材料,使用高通量测序平台获得其转录组数据,经质控后将其匹配到参考基因组,然后使用GATK软件检测和过滤SNP分子标记,并使用snpEff对SNP进行注释及统计分析.利用这些SNP分子标记进行系统发生分析、主成分分析和群体结构分析.最后,开发KASP标记并随机验证其有效性.[结果]转录组数据经质控后平均每个样本获得6.5 Gb的序列.通过变异分析和多重过滤筛选后,共获得220397个注释到染色体上的双等位基因SNP位点,平均密度为3 SNP/kb.SNP类型及分布特征分析结果表明,编码区错义突变与沉默突变的比值(N/S)为1.05,转换类型和颠换类型的比值(Ts/Tv)为1.89,杂合SNP占38.66％～49.91％,位于转录本的SNP比例为44.74％.系统发生分析、主成分分析和群体结构分析结果均表明,甘蔗栽培品种遗传来源较为单一,但群体分化较大.开发了11176个KASP分子标记,并随机合成25组KASP引物进行多态性验证,共有23组(92％)引物能检测到扩增产物,7组(28％)在40个甘蔗材料中呈现多态性,进一步验证了这些标记有效性.[结论]与传统SSR分子标记和基于GBS(Ge-notyping-by-sequencing)简化基因组测序的SNP分子标记开发方法相比,基于转录组测序的甘蔗SNP分子标记开发方法在保证质量和数量的情况下能获得更大比例的有效SNP,更有利于发掘功能SNP位点,为甘蔗分子SNP标记的开发提供了新的途径.

分离群体是进行作物遗传图谱构建、性状遗传研究、性状调控关键基因挖掘和种质资源创新利用的重要基础.本研究利用'崖城94-46'和'新台糖22号'杂交获得了209个杂交单株,通过SSR标记鉴定真假杂种和剔除病死单株,获得了1个包含145个杂交后代的F1群体.F1群体主要农艺性状遗传变异分析表明,8个性状都存在双向超亲现象,变异系数范围为9.23％～56.78％,8个性状的频数分布均呈连续正态分布,均为由多基因控制的数量性状.相关分析和逐步回归分析表明,甘蔗丛重与株高、茎径和丛有效茎显著正相关,决定系数为0.92;丛糖含量主要由株高、茎径、锤度和丛有效茎4个因素决定,决定系数为0.94.研究结果为利用甘蔗F1群体开展遗传研究奠定基础,同时为甘蔗育种中重点选择性状确定提供参考.

为探明不同农艺节水措施在甘蔗应对季节性干旱中的作用机制,本研究以'粤糖94-128'为材料,设置地膜覆盖+有机肥(T1)、保水剂+有机肥(T2)、蔗叶覆盖+有机肥(T3)、蔗叶覆盖+保水剂(T4)4个处理,以常规种植为对照(CK),研究不同农艺节水处理下土壤水分和出苗率、农艺性状和干物质积累、光合活性和根系指标以及经济性状对季节性干旱的响应.结果表明:干旱胁迫前期土壤含水量表现为地膜覆盖>蔗叶覆盖>无覆盖;T1处理出苗率最高且显著高于CK.2015年,与CK相比,T1处理显著增加株高(18.1％)和+1叶长(30.6％),T4处理显著增加茎粗(21.8％),而T2处理的株高、茎粗和叶宽则显著降低;T1处理显著增加+1叶的最大光能利用效率(Fv/Fm)和叶片SPAD值(SPAD-502叶绿素仪测定值),T3处理显著增加+1叶PSII实际光化学效率(ΦPSII),其余各处理与CK之间差异不显著;T1、T2、T3、T4处理的总干物质积累分别增加94.5％、–37.0％、53.4％、79.9％;T3处理显著增加根长密度、根表面积和根数,T2处理各根系指标则显著降低;T2和T3处理单茎重高于T1处理,但有效茎数远低于T1处理,有效茎数高是T1处理高产的主要原因;有机肥处理显著增加田间锤度,T3处理增幅最高.2016年,与CK相比,甘蔗不同处理的农艺性状、生理指标和干物质积累差异不显著;但与2015年相比,T1处理农艺性状指标呈下降趋势,而T2处理则呈上升趋势;各处理+1叶的ΦPSII均呈下降趋势;T1、T2和T3处理总干物质积累呈下降趋势,而T1和CK则呈上升趋势.T3处理根系生物量和根长密度显著高于CK,主要是由于细根增加所致;T4处理有效茎长显著高于CK,但茎径和单茎重并无显著差异,产量差异源于有效茎数的差异;有机肥处理显著增加田间锤度且各处理的田间锤度与2015年比呈上升趋势.综上,农艺节水措施主要通过在旱季减少土壤水分散失而提高甘蔗出苗率从而增加有效茎数,并通过增加植株干物质积累和促进根系生长以应对季节性干旱,进而保障后期经济性状.

分子标记缺乏是制约甘蔗分子标记技术发展的重要因素.本研究利用甘蔗AP85-441和R570基因组参考序列,使用MISA软件分别鉴定出512835和97839个微卫星位点,分别占割手密基因组(AP85-441)和甘蔗基因组(R570)高质量参考序列的0.32％和0.35％.在2个基因组序列中,优势重复单元均为单核苷酸、二核苷酸和三核苷酸,各重复单元均以AT富集的基元为主.割手密和甘蔗基因组序列中分别有472117和89748个位点可以开发SSR标记.对割手密、甘蔗与高粱基因进行同源性分析,分别鉴定出16691个和13271个对应到高粱1～10号染色体上的同源基因,利用基因序列中的SSR位点,开发出13224和7624对SSR引物.对开发的引物分别以割手密和甘蔗基因组为模板进行e-PCR检测,这些引物在割手密基因组中以多位点扩增为主,在2个基因组中的有效标记比例分别为79.35％和36.13％、79.01％和93.36％.部分SSR引物在基因组中表现出特异性扩增,有1368对仅在AP85-441中单扩增,有1420对仅在R570序列中单扩增,共有752对SSR引物可在2个基因组中单扩增,且这些SSR的扩增位点和来源基因均分布于所在基因组的全部染色体上.在禾本科作物基因组中e-PCR检测表明,开发的单扩增SSR标记具有较好的特异性.本研究鉴定的SSR位点,有助于进一步丰富甘蔗的分子标记;开发的3540对SSR引物对于栽培种甘蔗遗传图谱构建中遗传来源区分和同源连锁群确定具有重要的参考意义.

2016年于湛江对第五轮国家甘蔗品种集成示范的9个参试品种进行新植试验.结果表明:云蔗09-1601、桂糖44、粤甘46产量和蔗糖分均优于对照(ROC22);柳城07-150和福农09-4095产量或糖分低于对照,但含糖量高于对照(ROC22).这5个品种综合表现突出,可在湛江蔗区进一步扩大示范种植.

甘蔗砍收后堆放过程中,耐贮性与品种有直接关系.本研究以5个甘蔗品种(系)为供试材料,观测砍收后7 d内,其甘蔗蔗糖分、重力纯度、简纯度、还原糖分、纤维分、单茎重等指标变化情况.采用相关分析、因子分析法进行分析,对测定指标间相关性及5个甘蔗品种(系)品质变化进行评价.结果表明,随着堆放时间延长,甘蔗蔗糖分、重力纯度、简纯度均呈下降趋势,甘蔗纤维分、还原糖分和单茎重损失均呈上升趋势,重力纯度和简纯度指标间极显著正相关,还原糖分与简纯度指标间显著负相关;6个指标可简化为2个因子,其达到所有指标91.297％的信息量;利用因子得分对供试甘蔗品种(系)进行聚类分析,可分为3个类群.在5个品种(系)中,热甘11-1559耐贮性最好,ROC22和热甘14-291次之,热甘11-122和热甘12-60最差.

In order to investigate the performance of ratoon sugarcane harvested by mechanization and to screen out the varieties suitable for mechanized harvesting, we tested varieties from the national sugarcane research system, and analyzed the relationship between soil compaction and ratoon germination. The results indicated that the soil compaction of mechanical harvesting was significantly higher than that of manual harvesting; the relationship between soil compaction and ratoon germination was in significant negative correlation. Among the tested varieties, several varieties in the condition of higher soil compaction after mechanized harvest had relatively higher ratoon germination, including GuiTang31, FuNong38, LiuCheng05-136, LiuCheng03-1137 and YueGan24, showing the performance to adapt to the mechanical harvesting.

Sugarcane is a important crop in Zhanjiang. Sugarcane planting is the basic of sugarcane industry, and sugarcane growers play a key role in sugarcane production. In order to understand the status and influencing factors of sugarcane growers, 100 farmers was investigated through questionnaire survey. The practical condition was analyzed and some suggestions about the sugarcane industry were given. <br> Sugarcane is a important crop in Zhanjiang. Sugarcane planting is the basic of sugarcane industry, and sugarcane growers play a key role in sugarcane production. In order to understand the status and influencing factors of sugarcane growers, 100 farmers was investigated through questionnaire survey. The practical condition was analyzed and some suggestions about the sugarcane industry were given.

探讨切段式联合收割机收获的甘蔗茎段糖分变化情况,为今后糖厂合理安排机械砍收顺序和入榨时间提供参考.分别对两个甘蔗品种粤糖94-128和粤糖00-236采取人工收获和机械化收获,分析其放置过程中(5d内,自然条件下)蔗糖分、还原糖、重力纯度变化情况.结果表明:与人工收获方式相对比,两个品种在机械化切段式收获方式下的蔗糖分、还原糖、重力纯度在收获当天转化不显著,而从收获后第二天开始,糖分开始变化,具体为蔗糖分、重力纯度下降,还原糖分升高,粤糖00-236的糖分转化损失速度大于粤糖94-128.因此建议切段式收获的甘蔗茎段应该安排当天入榨,而在同一天砍收这两个品种的情况下应该优先安排粤糖00-236入榨.

【Objective】Cloning the 14-3-3 gene of sugarcane,analyzing and predicting its coding protein structure were condueted to provide references for gene function and metabolic regulatory mechanism studies of sugarcane.【Method】In the present study,a cDNA encoding 14-3-3 protein from Saccharum spp.hybrids was cloned and sequenced by RT-PCR technique based on comparing EST sequences of 14-3-3 protein found in sugarcane.The secondary structure,advanced structure and functional activity sites of 14-3-3 protein were predicted by bioinformatics.【Result】The cDNA length was 784 bp,28.88 kDa of molecular weight and 4.79 of isoelectric point.It contained 771 bp of open reading frame encoding a polypeptide of 256 amino acids.Cluster analysis revealed that the sugarcane polypeptide shared more than 90% identity with rice,broomcorn and maize.The binding motif for the target protein consisted of a basic pocket composed of Lys-50,Arg-57 and Arg-131,as well as Tyr-132,within the third and fifth helices.The activity sites for the phosphoserine were also shown in the 60th,65th,188thand 218thserines.Sugarcane 14-3-3 gene transcripts were detected by real time-PCR in different tissues.The results revealed that 14-3-3 gene could express in different tissues of sugarcane.It expressed highly in stalk and meristem,which may be related with glucose metabolism and cell division.In roots,sugarcane 14-3-3 maybe participate into absorption and metabolic of mineral elements.【Conclusion】Sugarcane 14-3-3 gene could act as one of the candidate genes for signal transduction regulating protein.