In this paper, a method for the rapid detection of diethylstilbestrol (DES) using quartz crystal microbalance( QCM) with gold nanoparticles (AuNPs) amplification was developed and this method was used on the arrayed langasite crystal microbalance (LCM) to realize the high-throughput detection of DES. A sensitive membrane which can recognize DES specifically was prepared on the surface of gold electrode through the molecular self-assembly technique. Gold nanoparticles (AuNPs) were coupled with anti-DES antibodies to amplify the signal of QCM. DES was detected through competitive immunoassay. At the same time, five gold electrodes were plated on a single langasite crystal in order to prepare an arrayed LCM sensor. The reliability and efficiency of the arrayed sensor were studied. The QCM sensor exhibits a linear detection range from 16 to 500 ng/mL and a detection limit of 13 ng/mL. The recovery rate of DES in the milk samples was 98.0-104.8%. By using structural analogs, the QCM sensor platform was demonstrated to be specific for the detection of DES. The arrayed LCM sensor was developed successfully and it could detect DES with arrayed method. (c) 2017 Elsevier B.V. All rights reserved.

To develop an up-converting phosphor technology based immunostrip assay for quantitative detection of estradiol.A composition contained up-converting phosphor (UCP) as a novel lable and estradiol antibody (E2-mAb) with glutaraldehyde method.The estradiol was detected by the fluorescent immunostrip assay based on indirect competitive principle which had this composition as a probe.Detection performance including stability,specificity and sensitivity was evaluated.The method showed well stability,specificity and sensitivity with the detection limit of 2.75 ng/mL,the linear range of 5 ng/mL to 2 000 ng/mL,the recovery of 88.88 ％ to 103.5 ％,and the coefficient of variation (CV) less than 10 ％.The established assay for E2 detection can be successfully used to fast-field analysis and has good economic value and broad application prospect.

The novel MIPs-coated UCNPs fluorescent sensor could be used for on-field detection of diethylstilbestrol in real samples.

The upconversion nanoparticles (UCNPs) NaYF4:Yb, Er were synthesized by the solvent thermal method. Fluorescence probe was prepared by coupling the anti-diethylstilbestrol (DES) monoclonal antibody with UCNPs. The DES-labeled bovine serum albumin (BSA) conjugates and goat anti-mouse antibodies were sprayed onto the nitrocellulose membrane as the test line and the control line, respectively. The resultant fluorescence probes were introduced into the immunochromatographic strip for rapid determination of DES. The UCNPs-based immunochromatographic strips for determination of DES exhibited a good linear range from 25 ng/mL to 10000 ng/mL (y = 0. 43927x-0. 57647, R-2 = 0. 996), with a limit of detection of 20. 84 ng/mL. The detection time of the proposed UCNPs based immunochromatographic strips for each sample was only 15 min. The RSDs of the intra and inter assay were less than 10%. The strip showed excellent specificity to structural and functional analogues, and the average RSD of detection value after storage at 37 degrees C for 7 days was about 15%. The recovery experiment showed that the average recoveries of DES at 5 spiked concentration levels were 90. 1% -115. 2%, and the RSDs were below 5%. These results were consistent with that of HPLC.

食品安全是关系到亿万人民健康生活的头等大事,保障食品安全的快速检测技术在不断进步.传统的理化检测方法存在样品前处理复杂、耗时费力、成本居高不下等问题,只能作为疑似样品的确证方法,不能满足质量安全监督现场筛查的需求.上转换发光免疫层析技术是建立在功能化上转换发光纳米材料与抗原抗体特异结合的层析技术基础上的一种新兴技术,具有灵敏度高、特异性强、使用便捷、检测速度快等特点,被广泛应用于快速检测过程中.本文对上转换发光技术及免疫层析技术进行了简要介绍,主要对上转换免疫层析技术以及其在食品安全检测领域的应用进展进行了综述.通过分析目前技术的优点与不足,根据目前的应用领域,提出了发展方向,为该技术在食品安全领域的进一步应用提供了参考.