The efficacy of a novel anti-tumor agent binaric catalitic system “teraphtal + ascorbic acid” [BCS (T+A)], generating reactive oxygen species, may depend on the activity of enzymes of the cellular antioxidant defense system including catalase (CAT). To evaluate the role of CAT in cancer cell defense from oxidative stress induced by BCS (T+A), we studied the following biomarkers: the expression level and basal activity of CAT in cells, and its sensitivity to specific inhibitor, aminotriazole (3-AT). We found that functionally active CAT was expressed constitutively in cultures of human tumor cells of different hystogenesis grown in vitro; at that the basal levels of CAT-protein expression and CAT-enzyme activity depend on cell types. The efficacy of CAT inhibiting by 3-AT (the parameter IC 50 3-АТ) is the same for cells of all tested cultures, it ranges from 20 to 25 mM and does not depend on the level of CAT protein expression in cells. No direct correlation was found between the biological CAT characteristics and their sensitivity to BCS (T+A) for cells of different hystogenesis. In case of pharmacological CAT inhibition, the cytotoxic activity of BCS (T+A) is doubled, whereas the human tumor cell sensitization degree (increased sensitivity) to BCS (T+A) depends on their type.

Purpose of this study is testing manganese and gadolinium sulpho-phthalocyanines aqueous water solutions as contrast agents for MRI. In the fields of 0,5 and 7 Tesla T1 relaxation times were measured and acceleration factors of relaxation for water aqueous solutions of proposed substances were calculated. MRI studies were having done in the field 7 Tesla in rats with C6 glioma before and after injection of proposed substances there were obtained images and T1-cards. The dynamics of tissue contrast was monitored. The measured acceleration factors of relaxation of proposed substances are comparable to the corresponding values for Magnevist TM. Reliable reduction of T1 for 1-2 hours is marked, which leads to a significant increase in signal from the tumor on T1-weighted images. In contrast to known agents drugs based on gadolinium chelates these solutions retain stay longer in the body, which allowing for more detailed MRI studies.

The authors propose a new heterogeneous photo sensitizer containing aluminum tetrakis [bis(cholinyl)phenylthio)] phthalocyanine grafted onto silica as an active phase. A poliovirus type 1 LSc2ab and RNA-containing phage MS2 model was used to show that the sensitizer had photo decontaminating activity against viruses and may be used to purify water from viral contamination. The mechanism for removal of viruses from water is two-step and involves the adsorption of the virus on the heterogeneous sensitizer particles and the photodynamic inactivation of the adsorbed virus.

The subject of the paper is study of optical absorption of sensitizers in biological tissue. The study shows that absorbance can be used as a tool that allows studying biodistribution of sensitizers and their interaction with tissue in vivo. The article presents a simple technique of determining biological tissue absorption in vivo, and discusses the results of experimental animal studies of some sensitizers.

The literature is becoming saturated with reports on the increase of the cytotocxicity of different anticancer or other chemical agents in combination with local ultrasound radiation of middle intensity (US). We used sonodinamics therapy to treat the transplanted intramuscular animal tumors. It was shown, that US alone increased the double time of tumor volume (t) more than 1,3-1,8 times. Sarcolysin, carboplatin, doxolem, 5-fluorouracil, methotrexat, and the new catalityc system of teraphtal+ascorbic acid were chosen to combine with sonodinamic agents. The additive or potential effect by the combined therapy was evaluated up to т >2,2-3,3.