ГИСТОЛОГО-БИОХИМИЧЕСКИЕ АСПЕКТЫ СОЧЕТАННОГО ВЛИЯНИЯ НЕКОТОРЫХ ЕСТЕСТВЕННЫХ МЕТАБОЛИТОВ НА ОБЩУЮ

Histone proteins are promising carriers for delivery of recombinant nucleic acids in various cell cultures. Conjugates of proteins with fluorescent dyes are modern tools in the study of cellular transport and intracellular distribution of important biopolymers. The aim of this work was to optimize the method of recombinant histone H1.3 conjugation with photoactivatable fluorescent dye. Also we studied intracellular penetration and subcellular localization of the resulting conjugate. Resulting conjugate was capable of intensive fluorescence in the red region of the spectrum after irradiation with violet light. The degree of conjugation was determined under different reaction conditions. A comparison of the cytotoxicity of histone H1.3 and its conjugate with photoactivated fluorescent dye in HeLa cells and its intracellular localization was characterized. We found that conjugates mainly localize in recycling endosomes and to a lesser extent in peroxisomes. Thus, the resulting conjugate of recombinant histone H1.3 with photoactivatable fluorescent dye can be used for further studies its anticancer activity and as carrier for drugs and nucleic acids delivery into human and animal cells.

Профилактика оксидативного стресса, оптимизирует интенсивность процессов липопероксидации, обуславливая высокую жизнеспособность эмбрионов перепелов, препятствуя замедлению развития особей даже в критические периоды эмбриогенеза, что приводит к естественной их акселерации.

Двукратная обработка яиц комплексом растворов препаратов коламина, янтарной кислоты и серина препятствовала развитию процессов липопероксидации у сельскохозяйственной птицы, обусловив положительные изменения в метаболизме и строении костной ткани суточных цыплят, что выразилось в снижении показателей перекисного окисления липидов, активизации ключевых ферментов, отвечающих за антиоксидантную защиту организма, а также в позитивном изменении динамики центральных маркеров костного матрикса.

Показано, что двукратная обработка инкубационных яиц комплексным раствором коламина, янтарной кислоты и серина способствовует снижению действия технологических стрессов и приводит к увеличению количества выведенного здорового молодняка и снижению затрат на производство продукции.

A novel photoactivated fluorescent dye is suitable for staining mitochondria and lysosomes in living human epidermoid carcinoma cells A431. These data were proved using laser scanning confocal microscopy with mitochondrial and lysosomal trackers as reference probes.

Novel method in diagnostics of illnesses and an estimation of the physiology-biochemical status of an organism is the measurement of a dynamic surface tension (DST) of serum of blood. DST has a number of features for horses of a different sex, age and breed. The stallions of different breeds have changes in different ages, that have features in 7-8 years. The most specifi c indicator of breed and age is the initial and fi nal slope of the tensiogram, that can be used in practice for express-control of age and breed of horses for blood samples. The highest values of surface tension for short times the existence of the surface for some groups of animals that may be associated with a particular ratio of components (proteins, lipids, salts, etc.) in the serum.