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1Опыт И Перспективы Использования Мобильных Лабораторий При Проведении Эпидемиологического Надзора За Чумой, Другими Особо Опасными, Природно-Очаговыми, Зоонозными Инфекционными Болезнями

И. Н. Шарова,Т. Ю. Красовская,Е. В. Казорина, Алексей Витальевич Казанцев, М. В. Проскурякова,В. Е. Куклев, Светлана Щербакова, Владимир Кутырев,Р. И. Адилов, Елизавета Владимировна Булычева, А. А. Троицкая,Б. Л. Агапов,

The review presents the materials on the use of mobile laboratories, developed in the Russian Federation, in the framework of four main areas: monitoring of territories to identify the circulation of pathogens of natural-focal infectious diseases; monitoring of territories during the exacerbation of the epizootic situation; participation in the elimination of outbreaks of infectious diseases; monitoring of territories to control and predict the epidemiological and epizootiologic situation in preparation for mass events. The tactical and technical characteristics and the procedure for organizing the operation of the mobile laboratory for monitoring and diagnostics mounted on the platform of the KamAZ chassis are considered. A distinctive feature of the laboratory from the existing Russian and foreign counterparts is the availability of the necessary conditions for conducting research using bacteriological analysis, rapid and accelerated diagnostic methods, and performing a full cycle of works – from the preparatory stage to the destruction of infected material. Hightech equipment allows the realization of two research schemes: the first – bacteriological analysis alongside the PCR, which ensures high reliability of the results; the second – conducting the PCR at the first stage, and when identifying genetic markers of the pathogen – performing bacteriological analysis of positive samples in order to isolate the culture of the pathogen and identify it subsequently. The second scheme will reduce the volume of bacteriological studies; make changes in the tactics of epizootiological survey. Tests of the mobile laboratory have demonstrated the effectiveness of its use in the epizootiological examination of natural plague foci, including cross-border ones. Owing to the use of a mobile laboratory in the Gorno-Altaisk high-mountain natural focus of plague, a new epizootic site was identified, located in a remote area, used by the population as a summer pasture for grazing domestic animals. The use of mobile laboratories will strengthen the laboratory base of institutions that monitor particularly dangerous, natural-focal and other dangerous infectious diseases; will help to bring the advanced diagnostic technologies directly to the natural focus, reduce the risk of epidemic complications due to plague and other particularly dangerous infections in cross-border natural foci.

2021

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2XXII Olympic Winter Games and XI Paralympic Winter Games 2014 in Sochi Sanitary and epidemiological welfare provision

Г.Г. Онищенко,А.Ю. Попова,Б.П. Кузькин,И.В. Брагина,,Е.Б. Ежлова,Ю.В. Демина,А.А. Горский,А.С. Гуськов,О.И. Аксенова,А.А. Мельникова,Н.Д. Пакскина,Г.Е. Иванов,

2015引用：2

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3Организация Диагностических Исследований В Мобильном Комплексе Специализированной Противоэпидемической Бригады В Период Проведения Массовых Мероприятий

Е. С. Казакова,С. А. Портенко, И. Н. Шарова, Игорь Карнаухов,Т. Ю. Красовская,В. Е. Куклев, Елизавета В. Найденова,Е. А. Билько,И. А. Касьян, Светлана Щербакова, А. В. Топорков

Laboratory support of epidemiological surveillance plays a significant role in the provision of sanitary-epidemiological welfare of the population at the time of preparations to and carrying out public events. Taking into consideration the increment of load upon the laboratory facilities of the Rospotrebnadzor institutions and general medical-and-prophylactic establishments, there emerges a need to deploy specialized anti-epidemic teams. By the example of management of the laboratory investigations in SAET mobile complex during XXVII World-wide Summer Universiade in Kazan, 2013 and G-20 Summit in Saint-Petersburg, 2013 formulated have been the basic principles of organization and algorithms of diagnostic work at the premises of laboratory facilities of SAET mobile unit deployed in order to provide for sanitary-epidemiological welfare of the population at the time of running public events.

2014

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4[Formation of Focus of West Nile Fever on the Territory of Saratov Region].

T Iu Krasovskaia,I N Sharova,E V Naĭdenova,V N Chekashov,S A Shcherbakova,E A Bil'ko,V E Kuklev,A N Matrosov,S A Iakovlev,A M Porshakov, M, A,

AIM:Detection of circulation of West Nile virus (WNV) on the territory of Saratov Region and prerequisites for formation of natural focus of West Nile fever (WNF), determination of the role of WNV in infectious pathology on the territory of the region.MATERIALS AND METHODS:of organs of small mammals, birds, blood-sucking arthropods for the presence of WNV markers (antigens and/or RNA) were studied. Clinical material from patients with symptoms not excluding WNF was studied. Donor blood sera samples were analyzed with the aim of detection of immune layer against WNV in population of Saratov Region.RESULTS:In 2010 WNV antigens were detected by EIA in 12 samples (7.1%) of mammal organ suspensions. In 2012 by using RT-PCR and EIA, markers of WNV were detected in 6 samples of bird brain suspensions (6.3%) and 1 sample of mammal organ suspension. Immune layer of population against WNV was 4% in 2011, 2.8% in 2012. In 2012 in 11 of 27 examined patients IgM against WNV in diagnostic titers and/or serconversion of IgG in paired sera were detected. In addition in 5 individuals virus RNA was detected in blood. Based on clinical, laboratory data and epidemiologic anamnesis 11 patients were diagnosed with WNF.CONCLUSION:The results obtained give evidence on the circulation of WNV on the territory of Saratov Region in 2010 - 2012. With the development of complications of WNF epidemiologic situation in 2012 an expansion of WNV areal onto the territory of the region took place and the process of formation of WNF natural foci is ongoing.

2013Zhurnal mikrobiologii, epidemiologii i immunobiologii（2013）引用：24

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5Первые случаи лихорадки Западного Нила на территории Саратовской области

Т. Ю. Красовская,Е. В. Найденова,Н. И. Миронова,Е. А. Талаева,В. Е. Куклев,Светлана Щербакова,Владимир Кутырев

2013Саратовский научно-медицинский журнал（2013）引用：23

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6Автоматизация Диагностических Исследований — Одно Из Направлений Стандартизации Лабораторной Диагностики Особо Опасных Инфекционных Болезней

С.А. Портенко,Е.С. Казакова,С.А. Щербакова,И.Н. Шарова,Д.В. Уткин,В.Е. Куклев,Е.А. Билько,Т.Ю. Красовская,И.А. Касьян

2012Russian Journal of Infection and Immunity（2012）

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7[Organization of Laboratory Mobile Complex of Specialized Antiepidemic Team of Institute for Plague Control].

E S Kazakova,I G Karnaukhov,I N Sharova,I A Kas'ian,N A Osina,S A Portenko, L N Ivashentseva, G A Kaplun,E V Kuklev,T Iu Krasovskaia,S A Shcherbakova,A V Toporkov,

2010Zhurnal mikrobiologii epidemiologii i immunobiologii（2010）引用：23

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8Полевые Испытания Мобильной Лаборатории Эпидразведки И Индикации. Сообщение 2. Полевые Испытания Мобильной Лаборатории Эпидразведки И Индикации На Территории Астраханской И Саратовской Областей, Республики Калмыкия

И Н Шарова,И В Кутырев,Т Ю Красовская,В Н Чекашов,А Н Матросов, М,А И Удовиков, В, А П Жуковская, Б М Бачаев, В Д Тюнникова, Санджиев В.Б-Х.,

2009Проблемы особо опасных инфекций（2009）

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9[Detection of Arbovirus Antigens in the Mosquitoes and Ticks Inhabiting the Saratov Region].

S A Shcherbakova,E A Bil'ko,E V Naĭdenova,I V Kutyrev,T Iu Krasovskaia,A a Sludskiĭ,T V Kniazeva,A N Matrosov,V N Chekashov,I N Sharova,L V Samoĭlova

The paper gives the results of a study dealing with the detection of the antigens of arboviruses of West Nile, Sindbis, Batai, Crimean-Congo hemorrhagic fever, a serocomplex of Californian encephalitis in the field material gathered in the Saratov Region in 2000-2006. The bloodsucking arthropods inhabiting the region were shown to be actively involved in the circulation of arboviruses in natural biotopes. The conclusion that it is expedient to organize an annual monitoring of arbovirus-induced infections in the areas where positive findings have been notified is justified.

2009Meditsinskaia parazitologiia i parazitarnye bolezni（2009）引用：23

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10Новые Данные О Распространении Иксодовых Клещей И Переносимых Ими Возбудителей Природно-Очаговых Инфекций В Саратовской Области