There is a lack of knowledge about longitudinal lung function trajectories in population-based COPD cohorts. The aim was to identify FEV1 trajectories and their determinants based on annual clinical examinations of individuals with airway obstruction sampled from the general population. In 2002-04, four population-based Swedish cohorts were invited to re-examinations including a structured interview and spirometry. All individuals with airway obstruction were identified (FEV1/VC<0.70, n=993), and thereafter invited to annual examinations for ≥10 years. Latent Class Mixed Models were used to derive FEV1 trajectories and their associations with time-varying covariates. Three trajectories were identified; highest FEV1 without decline (n=769, 51% men) intermediate FEV1 with rapid decline (n=162, 76% men) and lowest FEV1 without decline (n=62, 45% men) (Figure 1). The mean age was 64, 65 and 71 years, and the proportion never-smokers 28%, 3% and 24%, respectively, while mean BMI was similar in all groups. Sex, age, pack-years, and FEV1% of predicted at recruitment determined trajectory belonging, while pack-years, FEV1% of predicted at recruitment, and obesity during follow-up associated specifically to a more rapid FEV1 decline. Conclusion: There are different clinically important FEV1 trajectories in obstructive individuals that relate to modifiable factors including smoking and obesity.

The study presents investigation potentialities and risks of a liver biopsy method with a very fine needle (0.7 mm outer diameter). In 2 611 biopsies a serious complication was seen in one case, an intrahepatic haematoma, which had to be evacuated surgically. Otherwise even minor inconveniences to the patients proved exceptional. Specimens for cytological examination were obtained in 99% of the biopsies, and in 34 of 41 biopsies fragments of coherent liver tissue were obtained with a protein content varying from 32 to 900 μg . The specimens proved satisfactory for electron microscopy and under certain conditions also for chemical analysis of liver tissue.

Carboxypeptidase N (EC 3.4.17.3, arginine carboxypeptidase, kininase, anaphylatoxin inactivator) activity was determined in human sera with the method of Erdös by differential spectrometry at 254 nm and 37 degrees C, using hippuryl-L-argininic acid (HLAa) as a substrate. Day-to-day imprecision (CV) of the assay was 6.5%. The reference value, as determined in 15 healthy blood donors, was 3,082 +/- 432 IU/1. The mean value in a pool of sera from 12 healthy pregnant women, 4,500 +/- 294 IU/1, was higher in accordance with earlier findings by Erdös et al. Sera from 53 patients with chronic asthma or chronic urticaria, without known acetylsalicylic acid (ASA) intolerance, gave the mean value of 3,583 +/- 768 IU/1, and those from 17 patients with chronic asthma or chronic urticaria and ASA intolerance 3,379 +/- 575 IU/1. Thus, the carboxypeptidase-N activity in the blood of ASA-intolerant asthmatics and urticaria patients was unimpaired. A 16-year-old boy with asthma and without ASA intolerance showed an unusually low value of 2,056 IU/1.

Abstract. Liver biopsy specimens from 51 alcoholics obtained with a 0.7 mm needle have been analysed for lipid content and these values have been compared with the evaluation of visible fat in smears from the same biopsy specimens. Large variations in liver lipid concentrations have been found, ranging from one group, 24% of total, with fat within normal limits to a few individuals with a 100‐fold increase in liver lipid concentration. Partly this variation is explained by varying abstinence time before biopsy. A classification of liver lipid levels into three morphological categories is suggested and will give an adequate measure of this parameter. Serum transaminases and bilirubin were the only laboratory data found to co‐vary with liver fat.

. Fine needle biopsy specimens from patients with diseases accompanied by fatty liver have been analysed for triglycerides, phospholipids, free and esterified cholesterol. In selected cases the fatty acid composition of triglycerides was determined. The concentration of the lipids was not found to vary with the fundamental disease. A positive correlation between esterified cholesterol and triglycerides was verified in the presence of a moderately, but not of a severely, fatty liver. An association between esterified cholesterol and the surface area of fat droplets in the liver is suggested.

At a special hospital for severe oligophrenia all patients, 442 men and 155 women, and all members of the personnel, 688 persons, were examined for Au-antigen and antibodies against Au-antigen. 36 of the male patients proved to be Au-positive, and 33 of them were followed up for 6 months. None of the female patients or members of the personnel were Au-positive. One female patient and one female member of the personnel had antibodies. Liver biopsy specimens were obtained from 22 Au-positive and 12 Au-negative patients and necropsy specimens from 10 patients (1 Au-positive and 9 Au-negative) who had been tested for Au-antigen before death. In none of the specimens from the 23 Au-positive patients was the histological picture of the liver normal: in 15 the diagnosis was chronic persistent hepatitis, in 6 chronic aggressive hepatitis, and in 1 each cirrhosis and steatosis. Of the 21 Au-negative patients, the liver was found to be normal in 16 and to show mild or moderate signs of steatosis in 5.

Acta PaediatricaVolume 59, Issue 3 p. 293-296 FINE-NEEDLE ASPIRATION BIOPSY OF HUMAN LIVER FOR ENZYMATIC DIAGNOSIS OF GLYCOGEN STORAGE DISEASE AND GARGOYLISM A. LUNDQUIST, A. LUNDQUIST Departments of Internal Medicine and Clinical Chemistry, University Hospital, Lund, SwedenSearch for more papers by this authorP. A. ÖCKERMAN, P. A. ÖCKERMAN Departments of Internal Medicine and Clinical Chemistry, University Hospital, Lund, SwedenSearch for more papers by this author A. LUNDQUIST, A. LUNDQUIST Departments of Internal Medicine and Clinical Chemistry, University Hospital, Lund, SwedenSearch for more papers by this authorP. A. ÖCKERMAN, P. A. ÖCKERMAN Departments of Internal Medicine and Clinical Chemistry, University Hospital, Lund, SwedenSearch for more papers by this author First published: May 1970 https://doi.org/10.1111/j.1651-2227.1970.tb09006.xCitations: 8AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Citing Literature Volume59, Issue3May 1970Pages 293-296 RelatedInformation

Journal Article LIVER BIOPSY IN PSORIASIS Get access G. BERGE, G. BERGE Department of Dermatology, Department of Medicine and Department of Pathology, University of Lund, Lund, Sweden Search for other works by this author on: Oxford Academic Google Scholar A. LUNDQUIST, A. LUNDQUIST Department of Dermatology, Department of Medicine and Department of Pathology, University of Lund, Lund, Sweden Search for other works by this author on: Oxford Academic Google Scholar H. RORSMAN, H. RORSMAN Department of Dermatology, Department of Medicine and Department of Pathology, University of Lund, Lund, Sweden Search for other works by this author on: Oxford Academic Google Scholar M. ÅKERMAN M. ÅKERMAN Department of Dermatology, Department of Medicine and Department of Pathology, University of Lund, Lund, Sweden Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 82, Issue 3, 1 March 1970, Pages 250–253, https://doi.org/10.1111/j.1365-2133.1970.tb12432.x Published: 01 March 1970 Article history Accepted: 20 August 1969 Published: 01 March 1970

. The accuracy of fine-needle aspiration biopsy and cytodiagnosis in the detection of intrahepatic malignant tumours is evaluated. In a material of 1 748 biopsies, performed on varying and very wide indications, intrahepatic malignancy was diagnosed in 57 patients. Later, in 39 of these, cancer was histologically proved. There was no false positive cytodiagnosis of cancer. Fine-needle puncture detected about the same percentage of tumours as did angiography and scintigraphy. The method proved safe, simple and reliable for diagnosing intrahepatic cancer. It can be used either as an independent screening method for the detection of liver tumours, or for cytological analysis of localized foci detected by palpation, angiography, or scintigraphy. It is not invalidated by the hazards reported for conventional punch-needle biopsy when used in tumour-suspected cases.

Triglycerides and phospholipids 0.5-2mg (wet weight) of human liver material obtained by the fine-needle aspiration biopsy technique can be estimated after chloroform:methanol extraction by the determination of lipid phosphorus and glyceride glycerol. The methods are based on the colorimetric determination of inorganic phosphate after oxidation of the phospholipids and the enzymatic fluorometric determination of glycerol after complete hydrolysis of the triglycerides, catalyzed by purified pancreatic lipase. The accumulation of lipids is then expressed as the increase in the molar ratio of triglycerides over phospholipids.

Summary. The activity of five lysosomal acid hydrolases, β-galactosidase, β-acetylglucosaminidase, β-glucuronidase, acid phosphatase and α-mannosidase, was measured in liver biopsy specimens, obtained with the fine-needle aspiration technique from 24 carefully selected, strictly normal adults. Higher activities were noted than earlier described in less strictly normal controls.

Summary. By using fine-needle aspiration biopsy, liver tissue could be obtained from adults considered to be strictly healthy. The activity was measured of phosphorylase, amylo-1,6-glucosidase, acid α-glucosidase and glucose-6-phosphatase. The results obtained represent the first really normal values in the human liver of these enzyme activities. The activity of α-glucosidase was higher and that of the other enzymes similar to the values found earlier on less strictly normal controls. The possibility now available to define the normal range of enzyme values in human liver may facilitate the evaluation of enzyme analyses on liver tissue in disease.